{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:01:26Z","timestamp":1787162486344,"version":"build-2736575974"},"reference-count":63,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,4,30]],"date-time":"2023-04-30T00:00:00Z","timestamp":1682812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Horizon 2020 Research and Innovation Program","award":["737116"],"award-info":[{"award-number":["737116"]}]},{"name":"European Union\u2019s Horizon 2020 Research and Innovation Program","award":["PID2020-117024GB-C43"],"award-info":[{"award-number":["PID2020-117024GB-C43"]}]},{"name":"European Union\u2019s Horizon 2020 Research and Innovation Program","award":["NANOMAGCOST-CM P2018\/NMT-4321"],"award-info":[{"award-number":["NANOMAGCOST-CM P2018\/NMT-4321"]}]},{"name":"Spanish MCIN\/AEI\/10.13039\/501100011033","award":["737116"],"award-info":[{"award-number":["737116"]}]},{"name":"Spanish MCIN\/AEI\/10.13039\/501100011033","award":["PID2020-117024GB-C43"],"award-info":[{"award-number":["PID2020-117024GB-C43"]}]},{"name":"Spanish MCIN\/AEI\/10.13039\/501100011033","award":["NANOMAGCOST-CM P2018\/NMT-4321"],"award-info":[{"award-number":["NANOMAGCOST-CM P2018\/NMT-4321"]}]},{"DOI":"10.13039\/100012818","name":"Comunidad de Madrid","doi-asserted-by":"publisher","award":["737116"],"award-info":[{"award-number":["737116"]}],"id":[{"id":"10.13039\/100012818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012818","name":"Comunidad de Madrid","doi-asserted-by":"publisher","award":["PID2020-117024GB-C43"],"award-info":[{"award-number":["PID2020-117024GB-C43"]}],"id":[{"id":"10.13039\/100012818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012818","name":"Comunidad de Madrid","doi-asserted-by":"publisher","award":["NANOMAGCOST-CM P2018\/NMT-4321"],"award-info":[{"award-number":["NANOMAGCOST-CM P2018\/NMT-4321"]}],"id":[{"id":"10.13039\/100012818","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Nanomaterials design, synthesis, and characterization are ever-expanding approaches toward developing biodevices or neural interfaces to treat neurological diseases. The ability of nanomaterials features to tune neuronal networks\u2019 morphology or functionality is still under study. In this work, we unveil how interfacing mammalian brain cultured neurons and iron oxide nanowires\u2019 (NWs) orientation affect neuronal and glial densities and network activity. Iron oxide NWs were synthesized by electrodeposition, fixing the diameter to 100 nm and the length to 1 \u00b5m. Scanning electron microscopy, Raman, and contact angle measurements were performed to characterize the NWs\u2019 morphology, chemical composition, and hydrophilicity. Hippocampal cultures were seeded on NWs devices, and after 14 days, the cell morphology was studied by immunocytochemistry and confocal microscopy. Live calcium imaging was performed to study neuronal activity. Using random nanowires (R-NWs), higher neuronal and glial cell densities were obtained compared with the control and vertical nanowires (V-NWs), while using V-NWs, more stellate glial cells were found. R-NWs produced a reduction in neuronal activity, while V-NWs increased the neuronal network activity, possibly due to a higher neuronal maturity and a lower number of GABAergic neurons, respectively. These results highlight the potential of NWs manipulations to design ad hoc regenerative interfaces.<\/jats:p>","DOI":"10.3390\/biom13050783","type":"journal-article","created":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T13:54:46Z","timestamp":1682949286000},"page":"783","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Impact of Magnetite Nanowires on In Vitro Hippocampal Neural Networks"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8069-4908","authenticated-orcid":false,"given":"Bel\u00e9n","family":"Cort\u00e9s-Llanos","sequence":"first","affiliation":[{"name":"Departamento de F\u00edsica de Materiales, Universidad Complutense de Madrid, 28040 Madrid, Spain"},{"name":"Fundaci\u00f3n IMDEA Nanociencia, C\/Faraday 9, 28049 Madrid, Spain"},{"name":"Department of Medicine, Duke University, Durham, NC 27705, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8569-0810","authenticated-orcid":false,"given":"Rossana","family":"Rauti","sequence":"additional","affiliation":[{"name":"International School for Advanced Studies (ISAS-SISSA), 34136 Trieste, Italy"},{"name":"Deparment of Biomolecular Sciences, Universit\u00e0 degli Studi di Urbino Carlo Bo, 61029 Urbino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3919-5880","authenticated-orcid":false,"given":"\u00c1ngel","family":"Ayuso-Sacido","sequence":"additional","affiliation":[{"name":"Brain Tumor Laboratory, Fundaci\u00f3n Vithas, Grupo Hospitales Vithas, 28043 Madrid, Spain"},{"name":"Faculty of Experimental Science and Faculty of Medicine, University of Francisco de Vitoria, 28223 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9470-7987","authenticated-orcid":false,"given":"Lucas","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Departamento de F\u00edsica de Materiales, Universidad Complutense de Madrid, 28040 Madrid, Spain"},{"name":"Fundaci\u00f3n IMDEA Nanociencia, C\/Faraday 9, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8420-0787","authenticated-orcid":false,"given":"Laura","family":"Ballerini","sequence":"additional","affiliation":[{"name":"International School for Advanced Studies (ISAS-SISSA), 34136 Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"115","DOI":"10.5455\/bcp.20120621044747","article-title":"Nanotechnology Applications in Neuroscience: Advances, Opportunities and Challenges","volume":"22","author":"Cetin","year":"2012","journal-title":"Klin. Psikofarmakol. B\u00fclteni-Bull. Clin. Psychopharmacol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.pneurobio.2014.09.004","article-title":"Nanotechnologies for the study of the central nervous system","volume":"123","author":"Ajetunmobi","year":"2014","journal-title":"Prog. Neurobiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"953","DOI":"10.3389\/fnins.2018.00953","article-title":"Advances in Nano Neuroscience: From Nanomaterials to Nanotools","volume":"12","author":"Pampaloni","year":"2019","journal-title":"Front. Neurosci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"16093","DOI":"10.1038\/natrevmats.2016.93","article-title":"Neural recording and modulation technologies","volume":"2","author":"Chen","year":"2017","journal-title":"Nat. Rev. Mater."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jneumeth.2005.08.015","article-title":"Response of brain tissue to chronically implanted neural electrodes","volume":"148","author":"Polikov","year":"2005","journal-title":"J. Neurosci. Methods"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2108378","DOI":"10.1002\/adfm.202108378","article-title":"Ultra-Sharp Nanowire Arrays Natively Permeate, Record, and Stimulate Intracellular Activity in Neuronal and Cardiac Networks","volume":"32","author":"Liu","year":"2022","journal-title":"Adv. Funct. Mater."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1038\/nmat3404","article-title":"Macroporous nanowire nanoelectronic scaffolds for synthetic tissues","volume":"11","author":"Tian","year":"2012","journal-title":"Nat. Mater."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"29640","DOI":"10.1038\/srep29640","article-title":"The role of dimensionality in neuronal network dynamics","volume":"6","author":"Ban","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"91","DOI":"10.3389\/fnsys.2014.00091","article-title":"Nanostructures: A platform for brain repair and augmentation","volume":"8","author":"Vidu","year":"2014","journal-title":"Front. Syst. Neurosci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"345101","DOI":"10.1088\/0957-4484\/19\/34\/345101","article-title":"Axonal guidance on patterned free-standing nanowire surfaces","volume":"19","author":"Prinz","year":"2008","journal-title":"Nanotechnology"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"10966","DOI":"10.1073\/pnas.1411802111","article-title":"Molecular extraction in single live cells by sneaking in and out magnetic nanomaterials","volume":"111","author":"Yang","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"4020","DOI":"10.1021\/nl101950x","article-title":"Noninvasive Neuron Pinning with Nanopillar Arrays","volume":"10","author":"Xie","year":"2010","journal-title":"Nano Lett."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1148","DOI":"10.1002\/smll.201501896","article-title":"Control over Neurite Directionality and Neurite Elongation on Anisotropic Micropillar Arrays","volume":"12","author":"Park","year":"2016","journal-title":"Small"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"18944","DOI":"10.1021\/acsami.5b03798","article-title":"Support of Neuronal Growth Over Glial Growth and Guidance of Optic Nerve Axons by Vertical Nanowire Arrays","volume":"7","author":"Piret","year":"2015","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3542","DOI":"10.1021\/am300602e","article-title":"Highly Ordered Large-Scale Neuronal Networks of Individual Cells\u2013Toward Single Cell to 3D Nanowire Intracellular Interfaces","volume":"4","author":"Kwiat","year":"2012","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1038\/nnano.2013.273","article-title":"Free-standing kinked nanowire transistor probes for targeted intracellular recording in three dimensions","volume":"9","author":"Qing","year":"2014","journal-title":"Nat. Nanotech."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1038\/nnano.2011.249","article-title":"Vertical nanowire electrode arrays as a scalable platform for intracellular interfacing to neuronal circuits","volume":"7","author":"Robinson","year":"2012","journal-title":"Nat. Nanotech."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1038\/nnano.2012.8","article-title":"Intracellular recording of action potentials by nanopillar electroporation","volume":"7","author":"Xie","year":"2012","journal-title":"Nat. Nanotech."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5599","DOI":"10.1002\/adfm.201601817","article-title":"Biomechanical Cell Regulation by High Aspect Ratio Nanoimprinted Pillars","volume":"26","author":"Viela","year":"2016","journal-title":"Adv. Funct. Mater."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2757","DOI":"10.1021\/acs.nanolett.6b04752","article-title":"High Density Individually Addressable Nanowire Arrays Record Intracellular Activity from Primary Rodent and Human Stem Cell Derived Neurons","volume":"17","author":"Liu","year":"2017","journal-title":"Nano Lett."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"23158","DOI":"10.1021\/acs.jpcc.7b02625","article-title":"Thermal Route for the Synthesis of Maghemite\/Hematite Core\/Shell Nanowires","volume":"121","author":"Serrano","year":"2017","journal-title":"J. Phys. Chem. C"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1016\/j.yexcr.2009.12.016","article-title":"Cell guidance by magnetic nanowires","volume":"316","author":"Johansson","year":"2010","journal-title":"Exp. Cell Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2892","DOI":"10.1016\/j.actbio.2011.04.009","article-title":"Electroconductive polymeric nanowire templates facilitates in vitro C17.2 neural stem cell line adhesion, proliferation and differentiation","volume":"7","author":"Bechara","year":"2011","journal-title":"Acta Biomater."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2960","DOI":"10.1021\/nl070728e","article-title":"Gallium Phosphide Nanowires as a Substrate for Cultured Neurons","volume":"7","author":"Prinz","year":"2007","journal-title":"Nano Lett."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1007\/s11671-009-9498-0","article-title":"Coupling of Semiconductor Nanowires with Neurons and Their Interfacial Structure","volume":"5","author":"Lee","year":"2009","journal-title":"Nanoscale Res. Lett."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1021\/acs.nanolett.5b04458","article-title":"Axon-First Neuritogenesis on Vertical Nanowires","volume":"16","author":"Kang","year":"2016","journal-title":"Nano Lett."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"19892","DOI":"10.1021\/acsnano.2c08543","article-title":"Photoactive Nanomaterials for Wireless Neural Biomimetics, Stimulation, and Regeneration","volume":"16","author":"Zhao","year":"2022","journal-title":"ACS Nano"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"27888","DOI":"10.1039\/C4RA04121F","article-title":"Substrate porosity induces phenotypic alterations in retinal cells cultured on silicon nanowires","volume":"4","author":"Piret","year":"2014","journal-title":"RSC Adv."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3369","DOI":"10.1021\/acs.nanolett.6b05288","article-title":"Engineering Highly Interconnected Neuronal Networks on Nanowire Scaffolds","volume":"17","author":"Gautam","year":"2017","journal-title":"Nano Lett."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1700550","DOI":"10.1002\/adfm.201700550","article-title":"Nanostructures to Engineer 3D Neural-Interfaces: Directing Axonal Navigation toward Successful Bridging of Spinal Segments","volume":"28","author":"Aurand","year":"2018","journal-title":"Adv. Funct. Mater."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1800286","DOI":"10.1002\/adbi.201800286","article-title":"Carbon Nanotubes, Directly Grown on Supporting Surfaces, Improve Neuronal Activity in Hippocampal Neuronal Networks","volume":"3","author":"Rago","year":"2019","journal-title":"Adv. Biosyst."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"8879","DOI":"10.1021\/acsnano.9b02429","article-title":"Chemically Cross-Linked Carbon Nanotube Films Engineered to Control Neuronal Signaling","volume":"13","author":"Rauti","year":"2019","journal-title":"ACS Nano"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e61","DOI":"10.37175\/stemedicine.v1i4.61","article-title":"3D Carbon-based scaffolds for brain models and tissue engineering","volume":"1","author":"Severino","year":"2020","journal-title":"STEMedicine"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2002121","DOI":"10.1002\/admi.202002121","article-title":"Polystyrene Nanopillars with Inbuilt Carbon Nanotubes Enable Synaptic Modulation and Stimulation in Interfaced Neuronal Networks","volume":"8","author":"Calaresu","year":"2021","journal-title":"Adv. Mater. Interfaces"},{"key":"ref_35","first-page":"e2758347","article-title":"Biocompatible Carbon-Based Coating as Potential Endovascular Material for Stent Surface","volume":"2018","author":"Arkowski","year":"2018","journal-title":"BioMed Res. Int."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"24189","DOI":"10.1038\/srep24189","article-title":"Tunable magnetic nanowires for biomedical and harsh environment applications","volume":"6","author":"Ivanov","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Cort\u00e9s-Llanos, B., Ocampo, S.M., de la Cueva, L., Calvo, G.F., Belmonte-Beitia, J., P\u00e9rez, L., Salas, G., and Ayuso-Sacido, \u00c1. (2021). Influence of Coating and Size of Magnetic Nanoparticles on Cellular Uptake for In Vitro MRI. Nanomaterials, 11.","DOI":"10.3390\/nano11112888"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s12951-016-0190-0","article-title":"Iron oxide nanoparticles for neuronal cell applications: Uptake study and magnetic manipulations","volume":"14","author":"Marcus","year":"2016","journal-title":"J. Nanobiotechnol."},{"key":"ref_39","first-page":"47","article-title":"Upconversion Nanoparticles for Bioimaging and Regenerative Medicine","volume":"4","year":"2016","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"87","DOI":"10.7150\/ntno.21942","article-title":"Construction of Multifunctional Fe3O4-MTX@HBc Nanoparticles for MR Imaging and Photothermal Therapy\/Chemotherapy","volume":"2","author":"Zhang","year":"2018","journal-title":"Nanotheranostics"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"011310","DOI":"10.1063\/1.5121702","article-title":"Magnetic nanostructures for emerging biomedical applications","volume":"7","author":"Peixoto","year":"2020","journal-title":"Appl. Phys. Rev."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"233103","DOI":"10.1088\/0953-8984\/27\/23\/233103","article-title":"Interactions between semiconductor nanowires and living cells","volume":"27","author":"Prinz","year":"2015","journal-title":"J. Phys. Condens. Matter"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1021\/acs.nanolett.6b00020","article-title":"Spontaneous Internalization of Cell Penetrating Peptide-Modified Nanowires into Primary Neurons","volume":"16","author":"Lee","year":"2016","journal-title":"Nano Lett."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"121186","DOI":"10.1016\/j.biomaterials.2021.121186","article-title":"Nanostructured gold electrodes promote neural maturation and network connectivity","volume":"279","author":"Rosa","year":"2021","journal-title":"Biomaterials"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2000117","DOI":"10.1002\/adbi.202000117","article-title":"Interfacing Neurons with Nanostructured Electrodes Modulates Synaptic Circuit Features","volume":"4","author":"Rodilla","year":"2020","journal-title":"Adv. Biosyst."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"4459","DOI":"10.1021\/acsnano.6b00130","article-title":"Graphene Oxide Nanosheets Reshape Synaptic Function in Cultured Brain Networks","volume":"10","author":"Rauti","year":"2016","journal-title":"ACS Nano"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/S0006-8993(00)02922-X","article-title":"cAMP-induced stellation in primary astrocyte cultures with regional heterogeneity","volume":"887","author":"Won","year":"2000","journal-title":"Brain Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/glia.440110103","article-title":"Glutamate blocks astroglial stellation: Effect of glutamate uptake and volume changes","volume":"11","author":"Shao","year":"1994","journal-title":"Glia"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1007\/s00204-020-02659-9","article-title":"Bilirubin disrupts calcium homeostasis in neonatal hippocampal neurons: A new pathway of neurotoxicity","volume":"94","author":"Rauti","year":"2020","journal-title":"Arch. Toxicol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"9562","DOI":"10.1038\/srep09562","article-title":"From 2D to 3D: Novel nanostructured scaffolds to investigate signalling in reconstructed neuronal networks","volume":"5","author":"Bosi","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2408","DOI":"10.1016\/S0028-3908(00)00095-2","article-title":"Multi-neuronal recordings reveal a differential effect of thapsigargin on bicuculline- or gabazine-induced epileptiform excitability in rat hippocampal neuronal networks","volume":"39","author":"Sokal","year":"2000","journal-title":"Neuropharmacology"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"199","DOI":"10.3389\/fncir.2013.00199","article-title":"Identification of neuronal network properties from the spectral analysis of calcium imaging signals in neuronal cultures","volume":"7","author":"Tibau","year":"2013","journal-title":"Front. Neural Circuits"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Stetter, O., Battaglia, D., Soriano, J., and Geisel, T. (2012). Model-Free Reconstruction of Excitatory Neuronal Connectivity from Calcium Imaging Signals. PLoS Comput. Biol., 8.","DOI":"10.1371\/journal.pcbi.1002653"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.ceca.2006.07.006","article-title":"Activity-independent intracellular Ca2+ oscillations are spontaneously generated by ventral spinal neurons during development in vitro","volume":"41","author":"Fabbro","year":"2007","journal-title":"Cell Calcium"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"4184","DOI":"10.1021\/nl902413x","article-title":"Nanowire Biocompatibility in the Brain-Looking for a Needle in a 3D Stack","volume":"9","author":"Prinz","year":"2009","journal-title":"Nano Lett."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"43450","DOI":"10.1038\/srep43450","article-title":"Multifunctional Nano-engineered Polymer Surfaces with Enhanced Mechanical Resistance and Superhydrophobicity","volume":"7","author":"Viela","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"4021","DOI":"10.1038\/s41598-019-40548-z","article-title":"Cortical-like mini-columns of neuronal cells on zinc oxide nanowire surfaces","volume":"9","author":"Onesto","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1002\/smll.201001642","article-title":"Intact mammalian cell function on semiconductor nanowire arrays: New perspectives for cell-based biosensing","volume":"7","author":"Berthing","year":"2011","journal-title":"Small"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1038\/s41551-017-0154-1","article-title":"Glial responses to implanted electrodes in the brain","volume":"1","author":"Salatino","year":"2017","journal-title":"Nat. Biomed. Eng."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1038\/nature24638","article-title":"Astrocytic neuroligins control astrocyte morphogenesis and synaptogenesis","volume":"551","author":"Stogsdill","year":"2017","journal-title":"Nature"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1038\/nrn3725","article-title":"Astrocyte Ca2+ signalling: An unexpected complexity","volume":"15","author":"Volterra","year":"2014","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1152\/physrev.1998.78.1.247","article-title":"Functional significance of cell volume regulatory mechanisms","volume":"78","author":"Lang","year":"1998","journal-title":"Physiol. Rev."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1002\/glia.22626","article-title":"Dynamics of \u03b2-adrenergic\/cAMP signaling and morphological changes in cultured astrocytes","volume":"62","author":"Vardjan","year":"2014","journal-title":"Glia"}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/13\/5\/783\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:27:31Z","timestamp":1760124451000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/13\/5\/783"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,30]]},"references-count":63,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2023,5]]}},"alternative-id":["biom13050783"],"URL":"https:\/\/doi.org\/10.3390\/biom13050783","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,30]]}}}