{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T11:05:36Z","timestamp":1787828736583,"version":"build-2784847793"},"reference-count":201,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T00:00:00Z","timestamp":1685059200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"USDA National Institute of Food and Agriculture (NIFA) Award","award":["2018-67017-27632"],"award-info":[{"award-number":["2018-67017-27632"]}]},{"name":"NCSU College of Veterinary Medicine Department of Clinical Science Dissemination Fund","award":["2018-67017-27632"],"award-info":[{"award-number":["2018-67017-27632"]}]},{"name":"NCSU College of Veterinary Medicine Startup Funds (Sheats)","award":["2018-67017-27632"],"award-info":[{"award-number":["2018-67017-27632"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Neutrophils are important innate immune cells that respond during inflammation and infection. These migratory cells utilize \u03b22-integrin cell surface receptors to move out of the vasculature into inflamed tissues and to perform various anti-inflammatory responses. Although critical for fighting off infection, neutrophil responses can also become dysregulated and contribute to disease pathophysiology. In order to limit neutrophil-mediated damage, investigators have focused on \u03b22-integrins as potential therapeutic targets, but so far these strategies have failed in clinical trials. As the field continues to move forward, a better understanding of \u03b22-integrin function and signaling will aid the design of future therapeutics. Here, we provide a detailed review of resources, tools, experimental methods, and in vivo models that have been and will continue to be utilized to investigate the vitally important cell surface receptors, neutrophil \u03b22-integrins.<\/jats:p>","DOI":"10.3390\/biom13060892","type":"journal-article","created":{"date-parts":[[2023,5,27]],"date-time":"2023-05-27T16:19:37Z","timestamp":1685204377000},"page":"892","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Targeting Neutrophil \u03b22-Integrins: A Review of Relevant Resources, Tools, and Methods"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6370-6532","authenticated-orcid":false,"given":"Haleigh E.","family":"Conley","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA"},{"name":"Comparative Medicine Institute, North Carolina State University, Raleigh, NC 27607, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5270-4566","authenticated-orcid":false,"given":"M. Katie","family":"Sheats","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA"},{"name":"Comparative Medicine Institute, North Carolina State University, Raleigh, NC 27607, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1146\/annurev.immunol.021908.132554","article-title":"The Ins and Outs of Leukocyte Integrin Signaling","volume":"27","author":"Abram","year":"2009","journal-title":"Annu. Rev. Immunol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"619925","DOI":"10.3389\/fimmu.2020.619925","article-title":"\u03b22 Integrin Signaling Cascade in Neutrophils: More Than a Single Function","volume":"11","author":"Bouti","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1160\/TH07-02-0156","article-title":"Neutrophil activation via \u03b22 integrins (CD11\/CD18): Molecular mechanisms and clinical implications","volume":"98","author":"Schymeinsky","year":"2007","journal-title":"Thromb. Haemost."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"775447","DOI":"10.3389\/fimmu.2021.775447","article-title":"\u03b22 Integrin CD11d\/CD18: From Expression to an Emerging Role in Staged Leukocyte Migration","volume":"12","author":"Blythe","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"127","DOI":"10.3389\/fimmu.2012.00127","article-title":"Chemokines and the Signaling Modules Regulating Integrin Affinity","volume":"3","author":"Montresor","year":"2012","journal-title":"Front. Immunol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Grabbe, S., Wen, L., Lyu, Q., Ley, K., and Goult, B.T. (2022). Structural Basis of \u03b22 Integrin Inside\u2014Out Activation. Cells, 11.","DOI":"10.3390\/cells11193039"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1002\/jlb.65.6.725","article-title":"Integrin-mediated signaling in human neutrophil functioning","volume":"65","author":"Williams","year":"1999","journal-title":"J. Leukoc. Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/j.tcb.2007.09.001","article-title":"Immunoreceptor-like signaling by \u03b22 and \u03b23 integrins","volume":"17","author":"Jakus","year":"2007","journal-title":"Trends Cell Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.molimm.2012.11.006","article-title":"The molecular basis of leukocyte recruitment and its deficiencies","volume":"55","author":"Schmidt","year":"2013","journal-title":"Mol. Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.bj.2020.09.001","article-title":"Understanding the role of neutrophils in acute respiratory distress syndrome","volume":"44","author":"Yang","year":"2021","journal-title":"Biomed. J."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"8829674","DOI":"10.1155\/2020\/8829674","article-title":"COVID-19 and Neutrophils: The Relationship between Hyperinflammation and Neutrophil Extracellular Traps","volume":"2020","author":"Borges","year":"2020","journal-title":"Mediat. Inflamm."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1111\/imr.12373","article-title":"The many faces of Mac-1 in autoimmune disease","volume":"269","author":"Rosetti","year":"2016","journal-title":"Immunol. Rev."},{"key":"ref_13","first-page":"99","article-title":"The Leukocyte Cell Surface Receptor(s) for the iC3b Product of Complement","volume":"153","author":"Rosen","year":"1990","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"318","DOI":"10.4049\/jimmunol.1502381","article-title":"Integrin Cross-Talk Regulates the Human Neutrophil Response to Fungal \u03b2-Glucan in the Context of the Extracellular Matrix: A Prominent Role for VLA3 in the Antifungal Response","volume":"198","author":"Johnson","year":"2017","journal-title":"J. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1084\/jem.169.1.175","article-title":"CR3 (CD11b\/CD18) expresses one binding site for Arg-Gly-Asp-containing peptides and a second site for bacterial lipopolysaccharide","volume":"169","author":"Wright","year":"1989","journal-title":"J. Exp. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.pharmthera.2014.11.008","article-title":"Leukocyte integrins: Role in leukocyte recruitment and as therapeutic targets in inflammatory disease","volume":"147","author":"Mitroulis","year":"2015","journal-title":"Pharmacol. Ther."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2128","DOI":"10.2174\/138161208785740225","article-title":"Inhibitors Targeting the LFA-1\/ICAM-1 Cell-Adhesion Interaction: Design and Mechanism of Action","volume":"14","author":"Zimmerman","year":"2008","journal-title":"Curr. Pharm. Des."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Raab-Westphal, S., Marshall, J.F., and Goodman, S.L. (2017). Integrins as Therapeutic Targets: Successes and Cancers. Cancers, 9.","DOI":"10.3390\/cancers9090110"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1186\/s13075-021-02510-1","article-title":"Identification of neutrophil \u03b22-integrin LFA-1 as a potential mechanistic biomarker in ANCA-associated vasculitis via microarray and validation analyses","volume":"23","author":"Matsumoto","year":"2021","journal-title":"Arthritis Res. Ther."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"123","DOI":"10.3389\/fimmu.2019.00123","article-title":"CD11b Regulates Fungal Outgrowth but Not Neutrophil Recruitment in a Mouse Model of Invasive Pulmonary Aspergillosis","volume":"10","author":"Teschner","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1002\/JLB.4A0119-009RR","article-title":"Mac-1 triggers neutrophil DNA extracellular trap formation to Aspergillus fumigatus independently of PAD4 histone citrullination","volume":"107","author":"Silva","year":"2020","journal-title":"J. Leukoc. Biol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Friedrichs, K., Adam, M., Remane, L., Mollenhauer, M., Rudolph, V., Rudolph, T.K., Andri\u00e9, R.P., St\u00f6ckigt, F., Schrickel, J.W., and Ravekes, T. (2014). Induction of Atrial Fibrillation by Neutrophils Critically Depends on CD11b\/CD18 Integrins. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0089307"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"324","DOI":"10.4049\/jimmunol.1300858","article-title":"\u03b22 Integrin\u2013Mediated Crawling on Endothelial ICAM-1 and ICAM-2 Is a Prerequisite for Transcellular Neutrophil Diapedesis across the Inflamed Blood\u2013Brain Barrier","volume":"192","author":"Gorina","year":"2014","journal-title":"J. Immunol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"H1","DOI":"10.1530\/VB-19-0033","article-title":"Immune cell trafficking across the blood-brain barrier in the absence and presence of neuroinflammation","volume":"2","author":"Marchetti","year":"2020","journal-title":"Vasc. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1016\/j.chom.2011.10.009","article-title":"The \u03b2-Glucan Receptor Dectin-1 Activates the Integrin Mac-1 in Neutrophils via Vav Protein Signaling to Promote Candida albicans Clearance","volume":"10","author":"Li","year":"2011","journal-title":"Cell Host Microbe"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1183\/09031936.05.00086304","article-title":"Endothelial interactions of neutrophils under flow in chronic obstructive pulmonary disease","volume":"25","author":"Woolhouse","year":"2005","journal-title":"Eur. Respir. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1186\/1465-9921-14-47","article-title":"Adhesion molecules in subjects with COPD and healthy non-smokers: A cross sectional parallel group study","volume":"14","author":"Blidberg","year":"2013","journal-title":"Respir. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1186\/1465-9921-12-75","article-title":"Cigarette smoke induces \u03b22-integrin-dependent neutrophil migration across human endothelium","volume":"12","author":"Overbeek","year":"2011","journal-title":"Respir. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/s10067-015-3080-1","article-title":"Serum IL8 and mRNA level of CD11b in circulating neutrophils are increased in clinically amyopathic dermatomyositis with active interstitial lung disease","volume":"35","author":"Zou","year":"2016","journal-title":"Clin. Rheumatol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"540","DOI":"10.3389\/fnins.2019.00540","article-title":"The Inflammatory Response After Ischemic Stroke: Targeting \u03b22 and \u03b21 Integrins","volume":"13","author":"Edwards","year":"2019","journal-title":"Front. Neurosci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1084\/jem.20142358","article-title":"Blocking neutrophil integrin activation prevents ischemia\u2013reperfusion injury","volume":"212","author":"Yago","year":"2015","journal-title":"J. Exp. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"13899","DOI":"10.1038\/ncomms13899","article-title":"Prophylactic orthosteric inhibition of leukocyte integrin CD11b\/CD18 prevents long-term fibrotic kidney failure in cynomolgus monkeys","volume":"8","author":"Dehnadi","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.immuni.2015.11.011","article-title":"The Neutrophil Btk Signalosome Regulates Integrin Activation during Sterile Inflammation","volume":"44","author":"Volmering","year":"2016","journal-title":"Immunity"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1038\/nri2873","article-title":"Sterile inflammation: Sensing and reacting to damage","volume":"10","author":"Chen","year":"2010","journal-title":"Nat. Rev. Immunol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1725","DOI":"10.1172\/JCI119697","article-title":"Leukocyte adhesion deficiency type 1 (LAD-1)\/variant. A novel immunodeficiency syndrome characterized by dysfunctional beta2 integrins","volume":"100","author":"Kuijpers","year":"1997","journal-title":"J. Clin. Investig."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1182\/blood.2019003446","article-title":"Kindlin-3 recruitment to the plasma membrane precedes high-affinity \u03b22-integrin and neutrophil arrest from rolling","volume":"137","author":"Wen","year":"2021","journal-title":"Blood"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1038\/nm.1921","article-title":"Kindlin-3 is required for \u03b22 integrin\u2013mediated leukocyte adhesion to endothelial cells","volume":"15","author":"Moser","year":"2009","journal-title":"Nat. Med."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"109816","DOI":"10.1016\/j.cellsig.2020.109816","article-title":"Neutrophil signaling during myocardial infarction wound repair","volume":"77","author":"Daseke","year":"2021","journal-title":"Cell. Signal"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/S0735-1097(97)00424-5","article-title":"Increased Expression of Neutrophil and Monocyte Adhesion Molecules LFA-1 and Mac-1 and Their Ligand ICAM-1 and VLA-4 Throughout the Acute Phase of Myocardial Infarction: Possible Implications for Leukocyte Aggregation and Microvascular Plugging","volume":"31","author":"Meisel","year":"1998","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1038\/s41598-018-37852-5","article-title":"Autoimmune rheumatic disease IgG has differential effects upon neutrophil integrin activation that is modulated by the endothelium","volume":"9","author":"Khawaja","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1120398","DOI":"10.1038\/s41598-021-99893-7","article-title":"Integrin activation is an essential component of SARS-CoV-2 infection","volume":"11","author":"Simons","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"870","DOI":"10.3389\/fphar.2020.00870","article-title":"Neutrophilia and NETopathy as Key Pathologic Drivers of Progressive Lung Impairment in Patients With COVID-19","volume":"11","author":"Narasaraju","year":"2020","journal-title":"Front. Pharmacol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e00031-20","DOI":"10.1128\/IAI.00031-20","article-title":"Role of \u03b22 Integrins in Neutrophils and Sepsis","volume":"88","author":"Yuki","year":"2020","journal-title":"Infect. Immun."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1800786","DOI":"10.1183\/13993003.00786-2018","article-title":"Neutrophils disturb pulmonary microcirculation in sepsis-induced acute lung injury","volume":"53","author":"Park","year":"2019","journal-title":"Eur. Respir. J."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1038\/ni.1628","article-title":"Nonmuscle myosin light-chain kinase mediates neutrophil transmigration in sepsis-induced lung inflammation by activating \u03b22 integrins","volume":"9","author":"Xu","year":"2008","journal-title":"Nat. Immunol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1997","DOI":"10.1161\/CIRCULATIONAHA.107.724310","article-title":"Leukocyte Integrin Mac-1 Promotes Acute Cardiac Allograft Rejection","volume":"117","author":"Shimizu","year":"2008","journal-title":"Circulation"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1177\/0961203313491851","article-title":"The CD11b-integrin (ITGAM) and systemic lupus erythematosus","volume":"22","author":"Fagerholm","year":"2013","journal-title":"Lupus"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2907","DOI":"10.1002\/art.38117","article-title":"Multiple Lupus-AssociatedITGAMVariants Alter Mac-1 Functions on Neutrophils","volume":"65","author":"Zhou","year":"2013","journal-title":"Arthritis Rheum."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"15559","DOI":"10.1038\/ncomms15559","article-title":"Leukocyte integrin Mac-1 regulates thrombosis via interaction with platelet GPIb\u03b1","volume":"8","author":"Wang","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2939","DOI":"10.1111\/trf.13299","article-title":"Human neutrophil antigen-3a antibodies induce neutrophil stiffening and conformational activation of CD11b without shedding of L-selectin","volume":"55","author":"Berthold","year":"2015","journal-title":"Transfusion"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1097\/SHK.0b013e3182236eba","article-title":"Trauma-Activated Polymorphonucleated Leukocytes Damage Endothelial Progenitor Cells: Probable role of CD11b\/CD18-CD54 interaction and release of reactive oxygen specie","volume":"36","author":"Henrich","year":"2011","journal-title":"Shock"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"3789","DOI":"10.1182\/blood-2012-11-467985","article-title":"Extracellular protein disulfide isomerase regulates ligand-binding activity of \u03b1M\u03b22 integrin and neutrophil recruitment during vascular inflammation","volume":"121","author":"Hahm","year":"2013","journal-title":"Blood"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1097\/TA.0000000000003823","article-title":"Neutrophil heterogeneity and emergence of a distinct population of CD11b\/CD18-activated low-density neutrophils after trauma","volume":"94","author":"Joughin","year":"2023","journal-title":"J. Trauma Acute Care Surg."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.immuni.2006.06.014","article-title":"Impaired Integrin-Dependent Function in Wiskott-Aldrich Syndrome Protein-Deficient Murine and Human Neutrophils","volume":"25","author":"Zhang","year":"2006","journal-title":"Immunity"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/s10875-017-0453-z","article-title":"Clinical Manifestations and Pathophysiological Mechanisms of the Wiskott-Aldrich Syndrome","volume":"38","author":"Candotti","year":"2018","journal-title":"J. Clin. Immunol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1182\/blood-2010-01-259028","article-title":"In vivo labeling with 2H2O reveals a human neutrophil lifespan of 5.4 days","volume":"116","author":"Pillay","year":"2010","journal-title":"Blood"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1189\/jlb.1112571","article-title":"What\u2019s your age again? Determination of human neutrophil half-lives revisited","volume":"94","author":"Tak","year":"2013","journal-title":"J. Leukoc. Biol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1189\/jlb.5A0116-022R","article-title":"Low-density granulocytes: Functionally distinct, immature neutrophils in rheumatoid arthritis with altered properties and defective TNF signalling","volume":"101","author":"Wright","year":"2017","journal-title":"J. Leukoc. Biol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1254792","DOI":"10.1155\/2017\/1254792","article-title":"The Application of Dextran Sedimentation as an Initial Step in Neutrophil Purification Promotes Their Stimulation, due to the Presence of Monocytes","volume":"2017","author":"Quach","year":"2017","journal-title":"J. Immunol. Res."},{"key":"ref_60","first-page":"7.23.1","article-title":"Isolation and Functional Analysis of Human Neutrophils","volume":"111","author":"Kuhns","year":"2016","journal-title":"Curr. Protoc. Immunol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.vetimm.2017.04.011","article-title":"Effect of Mycoplasma bovis on bovine neutrophils","volume":"188","author":"Jimbo","year":"2017","journal-title":"Veter Immunol. Immunopathol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"538","DOI":"10.4049\/jimmunol.1100450","article-title":"Netting Neutrophils Induce Endothelial Damage, Infiltrate Tissues, and Expose Immunostimulatory Molecules in Systemic Lupus Erythematosus","volume":"187","author":"Villanueva","year":"2012","journal-title":"J. Immunol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"e1357","DOI":"10.1002\/cti2.1357","article-title":"Blunted sFasL signalling exacerbates TNF-driven neutrophil necroptosis in critically ill COVID-19 patients","volume":"10","author":"Schweizer","year":"2021","journal-title":"Clin. Transl. Immunol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/S0002-9440(10)61719-1","article-title":"A Novel Method for Isolation of Neutrophils from Murine Blood Using Negative Immunomagnetic Separation","volume":"159","author":"Cotter","year":"2001","journal-title":"Am. J. Pathol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.jim.2017.06.005","article-title":"Improved recovery of functionally active eosinophils and neutrophils using novel immunomagnetic technology","volume":"449","author":"Son","year":"2017","journal-title":"J. Immunol. Methods"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1189\/jlb.0102016","article-title":"A role for Syk-kinase in the control of the binding cycle of the \u03b22 integrins (CD11\/CD18) in human polymorphonuclear neutrophils","volume":"74","author":"Willeke","year":"2003","journal-title":"J. Leukoc. Biol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1182\/blood.V88.1.146.146","article-title":"Neutrophil Rolling, Arrest, and Transmigration Across Activated, Surface-Adherent Platelets Via Sequential Action of P-Selectin and the \u03b22-Integrin CDllb\/CDl8","volume":"88","author":"Diacovo","year":"1996","journal-title":"Blood"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1111\/j.1365-2141.1976.tb00179.x","article-title":"The cyropresevration of leukaemia cells: Morphological and functional changes","volume":"34","author":"Lowenthal","year":"1976","journal-title":"Br. J. Haematol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/0011-2240(81)90120-6","article-title":"Improved functional recovery of human granulocytes after cryopreservation","volume":"18","author":"Hill","year":"1981","journal-title":"Cryobiology"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1172\/JCI113939","article-title":"Cryopreservable neutrophil surrogates. Stored cytoplasts from human polymorphonuclear leukocytes retain chemotactic, phagocytic, and microbicidal function","volume":"83","author":"Malawista","year":"1989","journal-title":"J. Clin. Investig."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1182\/blood.V63.1.234.234","article-title":"Cryopreservation of enucleated human neutrophils (PMN cytoplasts)","volume":"63","author":"Voetman","year":"1984","journal-title":"Blood"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"e43","DOI":"10.4110\/in.2018.18.e43","article-title":"Extracellular Vesicles of Neutrophils","volume":"18","author":"Hong","year":"2018","journal-title":"Immune Netw."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Kolonics, F., Szeifert, V., Tim\u00e1r, C.I., Ligeti, E., and L\u0151rincz, \u00c1.M. (2020). The Functional Heterogeneity of Neutrophil-Derived Extracellular Vesicles Reflects the Status of the Parent Cell. Cells, 9.","DOI":"10.3390\/cells9122718"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Zhou, Y., and Br\u00e9chard, S. (2022). Neutrophil Extracellular Vesicles: A Delicate Balance between Pro-Inflammatory Responses and Anti-Inflammatory Therapies. Cells, 11.","DOI":"10.3390\/cells11203318"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"671995","DOI":"10.3389\/fimmu.2021.671995","article-title":"Mac-1 Receptor Clustering Initiates Production of Pro-Inflammatory, Antibacterial Extracellular Vesicles from Neutrophils","volume":"12","author":"Szeifert","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1182\/blood-2015-03-636720","article-title":"Interaction of kindlin-3 and \u03b22-integrins differentially regulates neutrophil recruitment and NET release in mice","volume":"126","author":"Xu","year":"2015","journal-title":"Blood"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1038\/nm.3887","article-title":"Diabetes primes neutrophils to undergo NETosis, which impairs wound healing","volume":"21","author":"Wong","year":"2015","journal-title":"Nat. Med."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1177\/1753425918817611","article-title":"Olfactomedin 4 marks a subset of neutrophils in mice","volume":"25","author":"Alder","year":"2018","journal-title":"Innate Immun."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Helou, D.G., Braham, S., De Chaisemartin, L., Granger, V., Damien, M.-H., Pallardy, M., Kerdine-R\u00f6mer, S., and Chollet-Martin, S. (2019). Nrf2 downregulates zymosan-induced neutrophil activation and modulates migration. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0216465"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.yjmcc.2018.08.029","article-title":"Role of neutrophils in CVB3 infection and viral myocarditis","volume":"125","author":"Rivadeneyra","year":"2018","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"2731","DOI":"10.4049\/jimmunol.172.5.2731","article-title":"Of mice and not men: Differences between mouse and human immunology","volume":"172","author":"Mestas","year":"2004","journal-title":"J. Immunol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1111\/imr.12350","article-title":"Mouse and human FcR effector functions","volume":"268","author":"Bruhns","year":"2015","journal-title":"Immunol. Rev."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"805","DOI":"10.3389\/fimmu.2019.00805","article-title":"IgG and Fc\u03b3 Receptors in Intestinal Immunity and Inflammation","volume":"10","author":"Clatworthy","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"4043","DOI":"10.4049\/jimmunol.1101651","article-title":"Protein Kinase C-\u03b8 Is Required for Murine Neutrophil Recruitment and Adhesion Strengthening under Flow","volume":"188","author":"Bertram","year":"2012","journal-title":"J. Immunol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1189\/jlb.0113040","article-title":"Technical Advance: Autofluorescence-based sorting: Rapid and nonperturbing isolation of ultrapure neutrophils to determine cytokine production","volume":"94","author":"Dorward","year":"2013","journal-title":"J. Leukoc. Biol."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"346","DOI":"10.3389\/fimmu.2019.00346","article-title":"Neutrophil Heterogeneity as Therapeutic Opportunity in Immune-Mediated Disease","volume":"10","author":"Nigrovic","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/j.it.2019.04.012","article-title":"Neutrophil Diversity in Health and Disease","volume":"40","author":"Fridlender","year":"2019","journal-title":"Trends Immunol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.trecan.2016.12.006","article-title":"Mouse versus Human Neutrophils in Cancer: A Major Knowledge Gap","volume":"3","author":"Eruslanov","year":"2017","journal-title":"Trends Cancer"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"2691","DOI":"10.1096\/fj.201900048R","article-title":"Neutrophil-endothelial interactions of murine cells is not a good predictor of their interactions in human cells","volume":"34","author":"Soroush","year":"2020","journal-title":"FASEB J."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/s13059-017-1156-8","article-title":"Genome-wide analysis of differential transcriptional and epigenetic variability across human immune cell types","volume":"18","author":"Ecker","year":"2017","journal-title":"Genome Biol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"100586","DOI":"10.1016\/j.blre.2019.06.003","article-title":"Benign ethnic neutropenia","volume":"37","author":"Ready","year":"2019","journal-title":"Blood Rev."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/j.tig.2019.04.003","article-title":"High-Diversity Mouse Populations for Complex Traits","volume":"35","author":"Saul","year":"2019","journal-title":"Trends Genet."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1002\/JLB.4A0816-354R","article-title":"Heat-shock protein-90 prolongs septic neutrophil survival by protecting c-Src kinase and caspase-8 from proteasomal degradation","volume":"103","author":"Gupta","year":"2018","journal-title":"J. Leukoc. Biol."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"5018","DOI":"10.1096\/fj.201802016RR","article-title":"Abrogation of cathepsin C by Helicobacter pylori impairs neutrophil activation to promote gastric infection","volume":"33","author":"Liu","year":"2019","journal-title":"FASEB J."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"513","DOI":"10.4049\/jimmunol.138.2.513","article-title":"Expression of leukocyte adherence-related glycoproteins during differentiation of HL-60 promyelocytic leukemia cells","volume":"138","author":"Hickstein","year":"1987","journal-title":"J. Immunol."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"22F.5.1","DOI":"10.1002\/0471142735.im22f05s104","article-title":"Differentiation and Characterization of Myeloid Cells","volume":"104","author":"Gupta","year":"2014","journal-title":"Curr. Protoc. Immunol."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"141","DOI":"10.2147\/JIR.S284941","article-title":"Studying Neutrophil Function in vitro: Cell Models and Environmental Factors","volume":"14","author":"Blanter","year":"2021","journal-title":"J. Inflamm. Res."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"6575","DOI":"10.4049\/jimmunol.0903910","article-title":"Vasodilator-Stimulated Phosphoprotein Regulates Inside-Out Signaling of \u03b22 Integrins in Neutrophils","volume":"184","author":"Deevi","year":"2010","journal-title":"J. Immunol."},{"key":"ref_99","doi-asserted-by":"crossref","unstructured":"Rinc\u00f3n, E., Rocha-Gregg, B.L., and Collins, S.R. (2018). A map of gene expression in neutrophil-like cell lines. BMC Genom., 19.","DOI":"10.1186\/s12864-018-4957-6"},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0145-2126(94)00063-G","article-title":"Functional properties of HL60 cells matured with all-trans-retinoic acid and DMSO: Differences in response to interleukin-8 and fMLP","volume":"19","author":"Sham","year":"1995","journal-title":"Leuk. Res."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1002\/cm.10101","article-title":"Expression of WASP and Scar1\/WAVE1 actin-associated proteins is differentially modulated during differentiation of HL-60 cells","volume":"54","author":"Launay","year":"2003","journal-title":"Cell Motil. Cytoskelet."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1016\/S1357-2725(02)00010-9","article-title":"Differentiated HL-60 cells are a valid model system for the analysis of human neutrophil migration and chemotaxis","volume":"34","author":"Hauert","year":"2002","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.yexcr.2004.07.015","article-title":"Differential inside-out activation of \u03b22-integrins by leukotriene B4 and fMLP in human neutrophils","volume":"300","author":"Patcha","year":"2004","journal-title":"Exp. Cell Res."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1182\/blood.V77.5.909.909","article-title":"Expression and regulation of myeloid-specific genes in normal and leukemic myeloid cells","volume":"77","author":"Lubbert","year":"1991","journal-title":"Blood"},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"3968","DOI":"10.4049\/jimmunol.159.8.3968","article-title":"Role of alpha L beta 2 integrin avidity in transendothelial chemotaxis of mononuclear cells","volume":"159","author":"Weber","year":"1997","journal-title":"J. Immunol. Methods"},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"10794","DOI":"10.1073\/pnas.1305121110","article-title":"Staphylococcus aureus LukAB cytotoxin kills human neutrophils by targeting the CD11b subunit of the integrin Mac-1","volume":"110","author":"DuMont","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_107","doi-asserted-by":"crossref","unstructured":"Xue, Z.-H., Feng, C., Liu, W.-L., and Tan, S.-M. (2013). A Role of Kindlin-3 in Integrin \u03b1M\u03b22 Outside-In Signaling and the Syk-Vav1-Rac1\/Cdc42 Signaling Axis. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0056911"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"6460","DOI":"10.4049\/jimmunol.0900983","article-title":"Outside-In Signal Transmission by Conformational Changes in Integrin Mac-1","volume":"183","author":"Lefort","year":"2009","journal-title":"J. Immunol."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"S214","DOI":"10.1097\/00003246-200205001-00007","article-title":"Leukocyte-endothelial interactions: Clinical trials of anti-adhesion therapy","volume":"30","author":"Harlan","year":"2002","journal-title":"Crit. Care Med."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1038\/78412","article-title":"CD18 trials disappoint again","volume":"18","author":"Dove","year":"2000","journal-title":"Nat. Biotechnol."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"3696","DOI":"10.1016\/j.bbagen.2013.02.018","article-title":"Small molecular agonists of integrin CD11b\/CD18 do not induce global conformational changes and are significantly better than activating antibodies in reducing vascular injury","volume":"1830","author":"Altintas","year":"2013","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"2517","DOI":"10.1016\/j.bpj.2013.10.020","article-title":"Agonist Leukadherin-1 Increases CD11b\/CD18-Dependent Adhesion Via Membrane Tethers","volume":"105","author":"Celik","year":"2013","journal-title":"Biophys. J."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1002\/JLB.1AB0618-232R","article-title":"HoxB8 neutrophils replicate Fc\u03b3 receptor and integrin-induced neutrophil signaling and functions","volume":"105","author":"Chu","year":"2019","journal-title":"J. Leukoc. Biol."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1002\/JLB.2A0818-314R","article-title":"Signaling and functional competency of neutrophils derived from bone-marrow cells expressing the ER-HOXB8 oncoprotein","volume":"106","author":"Saul","year":"2019","journal-title":"J. Leukoc. Biol."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"2142","DOI":"10.1038\/s41598-020-58882-y","article-title":"Context-Dependent Role of Vinculin in Neutrophil Adhesion, Motility and Trafficking","volume":"10","author":"Wilson","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"840894","DOI":"10.3389\/fcell.2022.840894","article-title":"Engraftment, Fate, and Function of HoxB8-Conditional Neutrophil Progenitors in the Unconditioned Murine Host","volume":"10","author":"Cohen","year":"2022","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"702345","DOI":"10.3389\/fimmu.2021.702345","article-title":"Binding of Rap1 and Riam to Talin1 Fine-Tune \u03b22 Integrin Activity During Leukocyte Trafficking","volume":"12","author":"Bromberger","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_118","doi-asserted-by":"crossref","unstructured":"Bromberger, T., Klapproth, S., Sperandio, M., and Moser, M. (2022). Humanized Beta2 Integrin-Expressing Hoxb8 Cells Serve as Model to Study Integrin Activation. Cells, 11.","DOI":"10.3390\/cells11091532"},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"C60","DOI":"10.1152\/ajpcell.1995.269.1.C60","article-title":"MCP-1-stimulated monocytes preferentially utilize \u03b22-integrins to migrate on laminin and fibronectin","volume":"269","author":"Penberthy","year":"1995","journal-title":"Am. J. Physiol."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1083\/jcb.120.6.1509","article-title":"Immune complex-stimulated neutrophil LTB4 production is dependent on \u03b22 integrins","volume":"120","author":"Graham","year":"1993","journal-title":"J. Cell Biol."},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1083\/jcb.126.4.1111","article-title":"\u03b22 Integrin-dependent Protein Tyrosine Phosphorylation and Activation of the FGR Protein Tyrosine Kinase in Human Neutrophils","volume":"126","author":"Berton","year":"1994","journal-title":"J. Cell Biol."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.4049\/jimmunol.173.3.2068","article-title":"Responses of Neutrophils to Anti-Integrin Antibodies Depends on Costimulation through Low Affinity Fc\u03b3Rs: Full Activation Requires Both Integrin and Nonintegrin Signals","volume":"173","author":"Jakus","year":"2004","journal-title":"J. Immunol."},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.vetimm.2014.04.009","article-title":"Myristoylated Alanine Rich C Kinase Substrate (MARCKS) is essential to \u03b22-integrin dependent responses of equine neutrophils","volume":"160","author":"Sheats","year":"2014","journal-title":"Veter Immunol. Immunopathol."},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/S0165-2427(01)00350-6","article-title":"Signaling mechanism for equine neutrophil activation by immune complexes","volume":"82","author":"Jones","year":"2001","journal-title":"Veter Immunol. Immunopathol."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1189\/jlb.1210708","article-title":"Endogenous PMN sialidase activity exposes activation epitope on CD11b\/CD18 which enhances its binding interaction with ICAM-1","volume":"90","author":"Feng","year":"2011","journal-title":"J. Leukoc. Biol."},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1002\/art.41057","article-title":"Increased Adhesive Potential of Antiphospholipid Syndrome Neutrophils Mediated by \u03b22 Integrin Mac-1","volume":"72","author":"Sule","year":"2020","journal-title":"Arthritis Rheumatol."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1165\/rcmb.2016-0215OC","article-title":"Activated \u03b22 Integrins Restrict Neutrophil Recruitment during Murine Acute Pseudomonal Pneumonia","volume":"56","author":"Wilson","year":"2017","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"678511","DOI":"10.3389\/fimmu.2021.678511","article-title":"Functional Homology for Antibody-Dependent Phagocytosis Across Humans and Rhesus Macaques","volume":"12","author":"Pollara","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"1855","DOI":"10.1096\/fasebj.13.13.1855","article-title":"A role for \u03b22 integrins (CD11\/CD18) in the regulation of cytokine gene expression of polymorphonuclear neutrophils during the inflammatory response","volume":"13","author":"Walzog","year":"1999","journal-title":"FASEB J."},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"2332","DOI":"10.1038\/s41598-017-02216-y","article-title":"Angiopoietin-1 enhances neutrophil chemotaxis in vitro and migration in vivo through interaction with CD18 and release of CCL4","volume":"7","author":"Burnett","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"3301","DOI":"10.4049\/jimmunol.164.6.3301","article-title":"Leukocyte Arrest During Cytokine-Dependent Inflammation In Vivo","volume":"164","author":"Kunkel","year":"2000","journal-title":"J. Immunol."},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"854","DOI":"10.4049\/jimmunol.155.2.854","article-title":"Activation of Lymphocyte Function-Associated Molecule4 (CDlla\/CD18) and Mac-1 (CD11 b\/CD18) Mimicked by an Antibody Directed Against CD18\u2019","volume":"155","author":"Petruzzelli","year":"1995","journal-title":"J. Immunol."},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"10556","DOI":"10.1074\/jbc.273.17.10556","article-title":"Two Signaling Mechanisms for Activation of \u03b1M\u03b22 Avidity in Polymorphonuclear Neutrophils","volume":"273","author":"Jones","year":"1998","journal-title":"J. Biol. Chem."},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1083\/jcb.120.2.545","article-title":"A Subpopulation of Mac-1 (CDllb\/CD18) Molecules Mediates Neutrophil Adhesion to ICAM-1 and Fibfinogen","volume":"120","author":"Diamond","year":"1993","journal-title":"J. Cell Biol."},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1016\/j.immuni.2006.07.016","article-title":"Activation of Leukocyte \u03b22 Integrins by Conversion from Bent to Extended Conformations","volume":"25","author":"Nishida","year":"2006","journal-title":"Immunity"},{"key":"ref_136","doi-asserted-by":"crossref","unstructured":"Fossati-Jimack, L., Ling, G.S., Cortini, A., Szajna, M., Malik, T.H., McDonald, J.U., Pickering, M.C., Cook, H.T., Taylor, P.R., and Botto, M. (2013). Phagocytosis Is the Main CR3-Mediated Function Affected by the Lupus-Associated Variant of CD11b in Human Myeloid Cells. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0057082"},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"1023865","DOI":"10.3389\/fimmu.2022.1023865","article-title":"Spatiotemporal characteristics of P-selectin-induced \u03b22 integrin activation of human neutrophils under flow","volume":"13","author":"Sun","year":"2022","journal-title":"Front. Immunol."},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1038\/s41385-020-0316-4","article-title":"Neutrophil expressed CD47 regulates CD11b\/CD18-dependent neutrophil transepithelial migration in the intestine in vivo","volume":"14","author":"Azcutia","year":"2021","journal-title":"Mucosal Immunol."},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"110876","DOI":"10.1016\/j.celrep.2022.110876","article-title":"A humanized \u03b22 integrin knockin mouse reveals localized intra- and extravascular neutrophil integrin activation in vivo","volume":"39","author":"Wen","year":"2022","journal-title":"Cell Rep."},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1114\/1.1511241","article-title":"Integrin activation by divalent ions affects neutrophil homotypic adhesion","volume":"30","author":"Spillmann","year":"2002","journal-title":"Ann. Biomed. Eng."},{"key":"ref_141","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1159\/000088560","article-title":"Intrapartum Magnesium Sulfate Exposure Attenuates Neutrophil Function in Preterm Neonates","volume":"89","author":"Mehta","year":"2006","journal-title":"Neonatology"},{"key":"ref_142","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1083\/jcb.133.4.895","article-title":"Deficiency of Src family kinases p59\/61hck and p58c-fgr results in defective adhesion-dependent neutrophil functions","volume":"133","author":"Lowell","year":"1996","journal-title":"J. Cell Biol."},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"4885","DOI":"10.1182\/blood-2010-05-287722","article-title":"Cleavage of the CD11b extracellular domain by the leukocyte serprocidins is critical for neutrophil detachment during chemotaxis","volume":"117","author":"Zen","year":"2011","journal-title":"Blood"},{"key":"ref_144","doi-asserted-by":"crossref","first-page":"4527","DOI":"10.1182\/blood-2008-12-195164","article-title":"The small Rho GTPase Cdc42 regulates neutrophil polarity via CD11b integrin signaling","volume":"114","author":"Szczur","year":"2009","journal-title":"Blood"},{"key":"ref_145","doi-asserted-by":"crossref","first-page":"2084","DOI":"10.1182\/blood-2012-08-449983","article-title":"Siglec-E is a negative regulator of acute pulmonary neutrophil inflammation and suppresses CD11b \u03b22-integrin\u2013dependent signaling","volume":"121","author":"McMillan","year":"2013","journal-title":"Blood"},{"key":"ref_146","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1002\/med.2610140203","article-title":"The pharmacology of the integrins","volume":"14","author":"Cox","year":"1994","journal-title":"Med. Res. Rev."},{"key":"ref_147","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1126\/science.2821619","article-title":"New Perspectives in Cell Adhesion: RGD and Integrins","volume":"238","author":"Ruoslahti","year":"1987","journal-title":"Science"},{"key":"ref_148","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/S1074-7613(02)00303-5","article-title":"Syk Is Required for Integrin Signaling in Neutrophils","volume":"16","author":"Zhou","year":"2002","journal-title":"Immunity"},{"key":"ref_149","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.1038\/ni1407","article-title":"Integrin signaling in neutrophils and macrophages uses adaptors containing immunoreceptor tyrosine-based activation motifs","volume":"7","author":"Abram","year":"2006","journal-title":"Nat. Immunol."},{"key":"ref_150","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/s12195-014-0322-2","article-title":"\u03b1V\u03b23 Integrin Regulation of Respiratory Burst in Fibrinogen Adherent Human Neutrophils","volume":"7","author":"Kim","year":"2014","journal-title":"Cell. Mol. Bioeng."},{"key":"ref_151","doi-asserted-by":"crossref","first-page":"2573","DOI":"10.1182\/blood-2013-07-516484","article-title":"Synchronized integrin engagement and chemokine activation is crucial in neutrophil extracellular trap-mediated sterile inflammation","volume":"123","author":"Rossaint","year":"2014","journal-title":"Blood"},{"key":"ref_152","doi-asserted-by":"crossref","first-page":"10680","DOI":"10.1073\/pnas.91.22.10680","article-title":"Identification of the complement iC3b binding site in the \u03b22 integrin CR3 (CD11b\/CD18)","volume":"91","author":"Ueda","year":"1994","journal-title":"Proc. Nati. Acad. Sci. USA"},{"key":"ref_153","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1073\/pnas.0405193102","article-title":"Myeloperoxidase mediates neutrophil activation by association with CD11b\/CD18 integrins","volume":"102","author":"Lau","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_154","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.4049\/jimmunol.160.3.1427","article-title":"The Human S100 Protein MRP-14 Is a Novel Activator of the \u03b22 Integrin Mac-1 on Neutrophils","volume":"160","author":"Newton","year":"1998","journal-title":"J. Immunol. Ref."},{"key":"ref_155","doi-asserted-by":"crossref","unstructured":"Monsel, A., L\u00e9cart, S., Roquilly, A., Broquet, A., Jacqueline, C., Mirault, T., Troude, T., Fontaine-Aupart, M.-P., and Asehnoune, K. (2014). Analysis of Autofluorescence in Polymorphonuclear Neutrophils: A New Tool for Early Infection Diagnosis. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0092564"},{"key":"ref_156","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.jneuroim.2011.11.009","article-title":"Neutrophils in multiple sclerosis are characterized by a primed phenotype","volume":"242","author":"Naegele","year":"2012","journal-title":"J. Neuroimmunol."},{"key":"ref_157","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1002\/cyto.a.22995","article-title":"Elimination of erroneous results in flow cytometry caused by antibody binding to Fc receptors on human monocytes and macrophages","volume":"89","author":"Andersen","year":"2016","journal-title":"Cytom. Part A"},{"key":"ref_158","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/978-1-0716-0154-9_10","article-title":"Using Imaging Flow Cytometry to Quantify Neutrophil Phagocytosis","volume":"2087","author":"Smirnov","year":"2020","journal-title":"Neutrophil. Methods Mol. Biol."},{"key":"ref_159","doi-asserted-by":"crossref","first-page":"4639","DOI":"10.1039\/C6LC01063F","article-title":"Review: Imaging technologies for flow cytometry","volume":"16","author":"Han","year":"2016","journal-title":"Lab Chip"},{"key":"ref_160","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1165\/rcmb.2008-0394OC","article-title":"Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) Protein Regulation of Human Neutrophil Migration","volume":"42","author":"Eckert","year":"2010","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"ref_161","first-page":"518","article-title":"Photodynamically induced endothelial cell injury and neutrophil-like HL-60 adhesion","volume":"9","author":"Takahashi","year":"2002","journal-title":"J. Photoscience"},{"key":"ref_162","doi-asserted-by":"crossref","first-page":"1867","DOI":"10.1083\/jcb.131.6.1867","article-title":"A role for phosphatidylinositol 3-kinase in the regulation of beta 1 integrin activity by the CD2 antigen","volume":"131","author":"Shimizu","year":"1995","journal-title":"J. Cell Biol."},{"key":"ref_163","doi-asserted-by":"crossref","first-page":"6718","DOI":"10.3390\/ijms16046718","article-title":"Understanding FRET as a Research Tool for Cellular Studies","volume":"16","author":"Shrestha","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"ref_164","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1002\/JLB.1A0720-471R","article-title":"Mitofusin-2 regulates leukocyte adhesion and \u03b22 integrin activation","volume":"111","author":"Liu","year":"2022","journal-title":"J. Leukoc. Biol."},{"key":"ref_165","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1016\/j.immuni.2007.04.011","article-title":"Syk signaling is necessary for E-selectin-induced LFA-1-ICAM-1 association and rolling but not arrest","volume":"26","author":"Zarbock","year":"2007","journal-title":"Immunity"},{"key":"ref_166","doi-asserted-by":"crossref","unstructured":"Morikis, V.A., Chen, S.J., Madigan, J., Jo, M.H., Werba, L.C., Ha, T., and Simon, S.I. (2022). \u03b22-Integrin Adhesive Bond Tension under Shear Stress Modulates Cytosolic Calcium Flux and Neutrophil Inflammatory Response. Cells, 11.","DOI":"10.3390\/cells11182822"},{"key":"ref_167","doi-asserted-by":"crossref","unstructured":"Posch, A. (2008). 2D PAGE: Sample Preparation and Fractionation, Humana Press.","DOI":"10.1007\/978-1-60327-210-0"},{"key":"ref_168","doi-asserted-by":"crossref","first-page":"3331","DOI":"10.1182\/blood-2010-06-289140","article-title":"The integrin LFA-1 signals through ZAP-70 to regulate expression of high-affinity LFA-1 on T lymphocytes","volume":"117","author":"Evans","year":"2011","journal-title":"Blood"},{"key":"ref_169","doi-asserted-by":"crossref","first-page":"3563","DOI":"10.1182\/blood-2012-04-426981","article-title":"Cdc42 regulates neutrophil migration via crosstalk between WASp, CD11b, and microtubules","volume":"120","author":"Kumar","year":"2012","journal-title":"Blood"},{"key":"ref_170","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.4049\/jimmunol.171.2.1042","article-title":"Modulation of CD11b\/CD18 Adhesive Activity by Its Extracellular, Membrane-Proximal Regions","volume":"171","author":"Xiong","year":"2003","journal-title":"J. Immunol."},{"key":"ref_171","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1182\/blood-2012-01-404046","article-title":"Ly6G ligation blocks recruitment of neutrophils via a \u03b22-integrin\u2013dependent mechanism","volume":"120","author":"Wang","year":"2012","journal-title":"Blood"},{"key":"ref_172","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1042\/bj20040151","article-title":"SRC-dependent outside-in signalling is a key step in the process of autoregulation of \u03b22 integrins in polymorphonuclear cells","volume":"380","author":"Piccardoni","year":"2004","journal-title":"Biochem. J."},{"key":"ref_173","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.imlet.2017.05.014","article-title":"The role of CR3 (CD11b\/CD18) and CR4 (CD11c\/CD18) in complement-mediated phagocytosis and podosome formation by human phagocytes","volume":"189","author":"Erdei","year":"2017","journal-title":"Immunol. Lett."},{"key":"ref_174","doi-asserted-by":"crossref","first-page":"3403","DOI":"10.1073\/pnas.1620881114","article-title":"Distinct recognition of complement iC3b by integrins \u03b1 X \u03b2 2 and \u03b1 M \u03b22","volume":"114","author":"Xu","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_175","doi-asserted-by":"crossref","first-page":"12658","DOI":"10.1038\/ncomms12658","article-title":"Neutrophil recruitment limited by high-affinity bent \u03b22 integrin binding ligand in cis","volume":"7","author":"Fan","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_176","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/s00281-014-0418-8","article-title":"The multiple faces of leukocyte interstitial migration","volume":"36","author":"Germain","year":"2014","journal-title":"Semin. Immunopathol."},{"key":"ref_177","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1242\/jcs.58.1.455","article-title":"Neutrophil granulocytes: Adhesion and locomotion on collagen substrata and in collagen matrices","volume":"58","author":"Brown","year":"1982","journal-title":"J. Cell Sci."},{"key":"ref_178","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1038\/ni.f.212","article-title":"Interstitial leukocyte migration and immune function","volume":"9","author":"Friedl","year":"2008","journal-title":"Nat. Immunol."},{"key":"ref_179","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1038\/nature06887","article-title":"Rapid leukocyte migration by integrin-independent flowing and squeezing","volume":"453","author":"Bader","year":"2008","journal-title":"Nature"},{"key":"ref_180","doi-asserted-by":"crossref","first-page":"3752","DOI":"10.1074\/jbc.M114.619643","article-title":"Matrix Confinement Plays a Pivotal Role in Regulating Neutrophil-generated Tractions, Speed, and Integrin Utilization","volume":"290","author":"Toyjanova","year":"2015","journal-title":"J. Biol. Chem."},{"key":"ref_181","doi-asserted-by":"crossref","first-page":"995","DOI":"10.4049\/jimmunol.163.2.995","article-title":"Effects of CD18 Deficiency on the Emigration of Murine Neutrophils During Pneumonia","volume":"163","author":"Mizgerd","year":"1999","journal-title":"J. Immunol."},{"key":"ref_182","doi-asserted-by":"crossref","first-page":"574","DOI":"10.3389\/fcimb.2022.894644","article-title":"Neutrophil Recruitment in Pneumococcal Pneumonia","volume":"12","author":"Palmer","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_183","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1165\/ajrcmb.23.2.f193","article-title":"CD11\/CD18-Dependent and -Independent Neutrophil Emigration in the Lungs: How Do Neutrophils Know Which Route to Take?","volume":"23","author":"Doerschuk","year":"2000","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"ref_184","first-page":"259","article-title":"Streptococcus pneumoniae-stimulated macrophages induce neutrophils to emigrate by a CD18-independent mechanism of adherence","volume":"31","author":"Mileski","year":"1990","journal-title":"Circ. Shock"},{"key":"ref_185","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1038\/nm.3913","article-title":"Neutrophils promote Alzheimer\u2019s disease\u2013like pathology and cognitive decline via LFA-1 integrin","volume":"21","author":"Zenaro","year":"2015","journal-title":"Nat. Med."},{"key":"ref_186","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1038\/sj.emboj.7601552","article-title":"A novel pathway of HMGB1-mediated inflammatory cell recruitment that requires Mac-1-integrin","volume":"26","author":"Orlova","year":"2007","journal-title":"EMBO J."},{"key":"ref_187","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1165\/rcmb.2017-0021OC","article-title":"Neutrophil-mediated Suppression of Influenza-induced Pathology Requires CD11b\/CD18 (MAC-1)","volume":"58","author":"Tak","year":"2018","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"ref_188","doi-asserted-by":"crossref","first-page":"5907","DOI":"10.4049\/jimmunol.1001533","article-title":"The Integrins Mac-1 and \u03b14\u03b21 Perform Crucial Roles in Neutrophil and T Cell Recruitment to Lungs during Streptococcus pneumoniae Infection","volume":"186","author":"Kadioglu","year":"2011","journal-title":"J. Immunol."},{"key":"ref_189","doi-asserted-by":"crossref","first-page":"823121","DOI":"10.3389\/fimmu.2022.823121","article-title":"Neutrophil-Specific Knockdown of \u03b22 Integrins Impairs Antifungal Effector Functions and Aggravates the Course of Invasive Pulmonal Aspergillosis","volume":"13","author":"Haist","year":"2022","journal-title":"Front. Immunol."},{"key":"ref_190","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/S0002-9440(10)63325-1","article-title":"Loss of LFA-1, but not Mac-1, Protects MRL\/MpJ-Faslpr Mice from Autoimmune Disease","volume":"165","author":"Kevil","year":"2004","journal-title":"Am. J. Pathol."},{"key":"ref_191","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1084\/jem.186.8.1357","article-title":"Neutrophil Emigration in the Skin, Lungs, and Peritoneum: Different Requirements for CD11\/CD18 Revealed by CD18-deficient Mice","volume":"186","author":"Mizgerd","year":"1997","journal-title":"J. Exp. Med."},{"key":"ref_192","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/S1074-7613(00)80278-2","article-title":"A Novel Role for the \u03b22 Integrin CD11b\/CD18 in Neutrophil Apoptosis: A Homeostatic Mechanism in Inflammation","volume":"5","author":"Coxon","year":"1996","journal-title":"Immunity"},{"key":"ref_193","first-page":"e60509","article-title":"Leukocyte Infiltration of Cremaster Muscle in Mice Assessed by Intravital Microscopy","volume":"2020","author":"Kranig","year":"2020","journal-title":"J. Vis. Exp."},{"key":"ref_194","doi-asserted-by":"crossref","first-page":"2569","DOI":"10.1084\/jem.20060925","article-title":"Intraluminal crawling of neutrophils to emigration sites: A molecularly distinct process from adhesion in the recruitment cascade","volume":"203","author":"Phillipson","year":"2006","journal-title":"J. Exp. Med."},{"key":"ref_195","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.jim.2015.08.012","article-title":"Visualization of integrin Mac-1 in vivo","volume":"426","author":"Lim","year":"2015","journal-title":"J. Immunol. Methods"},{"key":"ref_196","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1016\/j.it.2019.04.010","article-title":"Neutrophil Recruitment: From Model Systems to Tissue-Specific Patterns","volume":"40","author":"Margraf","year":"2019","journal-title":"Trends Immunol."},{"key":"ref_197","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.4049\/jimmunol.1400478","article-title":"Immobilized Immune Complexes Induce Neutrophil Extracellular Trap Release by Human Neutrophil Granulocytes via Fc\u03b3RIIIB and Mac-1","volume":"193","author":"Behnen","year":"2014","journal-title":"J. Immunol."},{"key":"ref_198","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1083\/jcb.144.5.1047","article-title":"Differential regulation of beta1 integrins by chemoattractants regulates neutrophil migration through fibrin","volume":"144","author":"Loike","year":"1999","journal-title":"J. Cell Biol."},{"key":"ref_199","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.bpj.2008.10.001","article-title":"Adhesion Between Human Neutrophils and Immobilized Endothelial Ligand Vascular Cell Adhesion Molecule 1: Divalent Ion Effects","volume":"96","author":"Lomakina","year":"2009","journal-title":"Biophys. J."},{"key":"ref_200","doi-asserted-by":"crossref","first-page":"2251","DOI":"10.1172\/JCI116111","article-title":"Entactin stimulates neutrophil adhesion and chemotaxis through interactions between its Arg-Gly-Asp (RGD) domain and the leukocyte response integrin","volume":"90","author":"Senior","year":"1992","journal-title":"J. Clin. Investig."},{"key":"ref_201","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1042\/bj3040537","article-title":"The vitronectin receptor (\u03b1v\u03b23) is implicated, in cooperation with P-selectin and platelet-activating factor, in the adhesion of monocytes to activated endothelial cells","volume":"304","author":"Murphy","year":"1994","journal-title":"Biochem. J."}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/13\/6\/892\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:43:00Z","timestamp":1760125380000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/13\/6\/892"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,26]]},"references-count":201,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["biom13060892"],"URL":"https:\/\/doi.org\/10.3390\/biom13060892","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,26]]}}}