{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T16:04:01Z","timestamp":1771949041581,"version":"3.50.1"},"reference-count":69,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,7,1]],"date-time":"2023-07-01T00:00:00Z","timestamp":1688169600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH National Eye Institute grants","award":["P30 EY031631"],"award-info":[{"award-number":["P30 EY031631"]}]},{"name":"NIH National Eye Institute grants","award":["R01EY021747"],"award-info":[{"award-number":["R01EY021747"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Delayed or prolonged corneal wound healing and non-healing corneas put patients at risk for ocular surface infections and subsequent stromal opacification, resulting in discomfort or visual loss. It is important to enhance corneal wound healing efficiency and quality. Vitamin D (Vit D) is both a hormone and a vitamin, and its insufficiency has been linked to immune disorders and diabetes. For this study, wound healing and recruitment of CD45+ cells into the wound area of normoglycemic and diabetic mice were examined following corneal epithelial debridement and treatment with 1,25-dihyroxyvitamin D (1,25 Vit D) or 24,25-dihydroxyvitamin D (24,25 Vit D). Treatment with topical 1,25-dihyroxyvitamin D (1,25 Vit D) resulted in significantly increased corneal wound healing rates of normoglycemic, diabetic and diabetic Vit D deficient mice. Furthermore, 24,25-dihydroxyvitamin D (24,25 Vit D) significantly increased corneal wound healing of diabetic Vit D deficient and Vit D receptor knockout (VDR KO) mice. In addition, CD45+ cell numbers were reduced in diabetic and VDR KO mouse corneas compared to normoglycemic mice, and 24,25 Vit D increased the recruitment of CD45+ cells to diabetic mouse corneas after epithelial debridement. CD45+ cells were found to infiltrate into the corneal basal epithelial layer after corneal epithelial debridement. Our data indicate that topical Vit D promotes corneal wound healing and further supports previous work that the Vit D corneal wound healing effect is not totally VDR-dependent.<\/jats:p>","DOI":"10.3390\/biom13071065","type":"journal-article","created":{"date-parts":[[2023,7,3]],"date-time":"2023-07-03T00:34:08Z","timestamp":1688344448000},"page":"1065","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Effects of Topical 1,25 and 24,25 Vitamin D on Diabetic, Vitamin D Deficient and Vitamin D Receptor Knockout Mouse Corneal Wound Healing"],"prefix":"10.3390","volume":"13","author":[{"given":"Xiaowen","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta University, Augusta, GA 30912, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta University, Augusta, GA 30912, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerry","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta University, Augusta, GA 30912, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2079-9887","authenticated-orcid":false,"given":"Mitchell","family":"Watsky","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta University, Augusta, GA 30912, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"107709","DOI":"10.1016\/j.exer.2019.107709","article-title":"Corneal injury: Clinical and molecular aspects","volume":"186","author":"Barrientez","year":"2019","journal-title":"Exp. Eye Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e000779","DOI":"10.1136\/bmjdrc-2019-000779","article-title":"Experimental modeling of cornea wound healing in diabetes: Clinical applications and beyond","volume":"7","author":"Bu","year":"2019","journal-title":"BMJ Open Diabetes Res. Care"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"981","DOI":"10.2147\/OPTH.S145266","article-title":"Diabetic corneal neuropathy: Clinical perspectives","volume":"12","author":"Bikbova","year":"2018","journal-title":"Clin. Ophthalmol."},{"key":"ref_4","first-page":"263","article-title":"Corneal nerves in health and disease","volume":"59","author":"Dhillon","year":"2019","journal-title":"Prog. Retin. Eye Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1016\/j.survophthal.2020.02.005","article-title":"Diabetic keratopathy: Insights and challenges","volume":"65","author":"Priyadarsini","year":"2020","journal-title":"Surv. Ophthalmol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4444","DOI":"10.1039\/C9BM00456D","article-title":"Biomaterials and controlled release strategy for epithelial wound healing","volume":"7","author":"Son","year":"2019","journal-title":"Biomater. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.addr.2018.01.006","article-title":"Wound healing in the eye: Therapeutic prospects","volume":"126","author":"Ziaei","year":"2018","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_8","first-page":"V","article-title":"Vitamin d deficiency\u2014An ignored epidemic","volume":"4","author":"Naeem","year":"2010","journal-title":"Int. J. Health Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"497","DOI":"10.17219\/acem\/37208","article-title":"The role of vitamin D3 in inflammatory bowel diseases","volume":"23","year":"2014","journal-title":"Adv. Clin. Exp. Med."},{"key":"ref_10","first-page":"244","article-title":"Vitamin D in inflammatory diseases","volume":"5","author":"Wobke","year":"2014","journal-title":"Front. Physiol."},{"key":"ref_11","first-page":"69","article-title":"Vitamin D and inflammatory diseases","volume":"7","author":"Yin","year":"2014","journal-title":"J. Inflamm. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"S11","DOI":"10.1111\/hdi.12347","article-title":"Study of the effect of vitamin D supplementation on glycemic control in type 2 diabetic prevalent hemodialysis patients","volume":"19","author":"Ibrahim","year":"2015","journal-title":"Hemodial. Int."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3264","DOI":"10.3390\/nu7053264","article-title":"Vitamin D as a Resilience Factor, Helpful for Survival of Potentially Fatal Conditions: A Hypothesis Emerging from Recent Findings of the ESTHER Cohort Study and the CHANCES Consortium","volume":"7","author":"Schottker","year":"2015","journal-title":"Nutrients"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1730","DOI":"10.1001\/archinte.167.16.1730","article-title":"Vitamin D supplementation and total mortality: A meta-analysis of randomized controlled trials","volume":"167","author":"Autier","year":"2007","journal-title":"Arch. Intern. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1001\/archinternmed.2008.604","article-title":"Demographic differences and trends of vitamin D insufficiency in the US population, 1988\u20132004","volume":"169","author":"Ginde","year":"2009","journal-title":"Arch. Intern. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.4103\/ijo.IJO_238_18","article-title":"Corneal endothelial changes in patients with vitamin D deficiency","volume":"66","author":"Cankaya","year":"2018","journal-title":"Indian J. Ophthalmol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4369750","DOI":"10.1155\/2016\/4369750","article-title":"Corneal Dendritic Cell Density Is Associated with Subbasal Nerve Plexus Features, Ocular Surface Disease Index, and Serum Vitamin D in Evaporative Dry Eye Disease","volume":"2016","author":"Shetty","year":"2016","journal-title":"Biomed. Res. Int."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1097\/ICO.0000000000000785","article-title":"Bowman Break and Subbasal Nerve Plexus Changes in a Patient With Dry Eye Presenting With Chronic Ocular Pain and Vitamin D Deficiency","volume":"35","author":"Shetty","year":"2016","journal-title":"Cornea"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"7359","DOI":"10.1167\/iovs.11-7605","article-title":"Vitamin D enhances corneal epithelial barrier function","volume":"52","author":"Yin","year":"2011","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4083","DOI":"10.1007\/s11033-021-06418-6","article-title":"Dose-dependent therapeutic effects of topical 1,25 OH-vitamin D3 on corneal wound healing","volume":"48","author":"Jabbehdari","year":"2021","journal-title":"Mol. Biol. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"5245","DOI":"10.1167\/iovs.13-13553","article-title":"Effect of vitamin D receptor knockout on cornea epithelium wound healing and tight junctions","volume":"55","author":"Elizondo","year":"2014","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.2337\/db19-1051","article-title":"Effects of Vitamin D Receptor Knockout and Vitamin D Deficiency on Corneal Epithelial Wound Healing and Nerve Density in Diabetic Mice","volume":"69","author":"Lu","year":"2020","journal-title":"Diabetes"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"107776","DOI":"10.1016\/j.exer.2019.107776","article-title":"Vitamin D receptor and metabolite effects on corneal epithelial cell gap junction proteins","volume":"187","author":"Lu","year":"2019","journal-title":"Exp. Eye Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"4074","DOI":"10.1167\/iovs.19-27796","article-title":"Influence of Vitamin D on Corneal Epithelial Cell Desmosomes and Hemidesmosomes","volume":"60","author":"Lu","year":"2019","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_25","first-page":"154","article-title":"Effects of 1alpha,25-dihydroxyvitamin D3 on Langerhans cell migration and corneal neovascularization in mice","volume":"41","author":"Suzuki","year":"2000","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1046\/j.1440-1711.80.4august.1.x","article-title":"1 alpha,25-Dihydroxyvitamin D3 inhibits pro-inflammatory cytokine and chemokine expression in human corneal epithelial cells colonized with Pseudomonas aeruginosa","volume":"80","author":"Xue","year":"2002","journal-title":"Immunol. Cell Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1167\/tvst.9.3.26","article-title":"Reproducible Derivation and Expansion of Corneal Mesenchymal Stromal Cells for Therapeutic Applications","volume":"9","author":"Jabbehdari","year":"2020","journal-title":"Transl. Vis. Sci. Technol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1947","DOI":"10.1167\/iovs.05-1193","article-title":"Two waves of neutrophil emigration in response to corneal epithelial abrasion: Distinct adhesion molecule requirements","volume":"47","author":"Li","year":"2006","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1007\/s00441-009-0908-5","article-title":"Neutrophils promote inflammatory angiogenesis via release of preformed VEGF in an in vivo corneal model","volume":"339","author":"Gong","year":"2010","journal-title":"Cell Tissue Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.exer.2016.11.006","article-title":"IL-20 promotes epithelial healing of the injured mouse cornea","volume":"154","author":"Zhang","year":"2017","journal-title":"Exp. Eye Res."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Reins, R.Y., Hanlon, S.D., Magadi, S., and McDermott, A.M. (2016). Effects of Topically Applied Vitamin D during Corneal Wound Healing. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0152889"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"E844","DOI":"10.1152\/ajpendo.00029.2011","article-title":"Inactivation of TNF-alpha ameliorates diabetic neuropathy in mice","volume":"301","author":"Yamakawa","year":"2011","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1097\/ICO.0000000000000089","article-title":"Effect of a Mediterranean dietary pattern and vitamin D levels on Dry Eye syndrome","volume":"33","author":"Galor","year":"2014","journal-title":"Cornea"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/1756-185X.12727","article-title":"Dry eye in vitamin D deficiency: More than an incidental association","volume":"19","author":"Yildirim","year":"2015","journal-title":"Int. J. Rheum. Dis."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1038\/eye.2015.96","article-title":"The association of vitamin D deficiency with tear break-up time and Schirmer testing in non-Sjogren dry eye","volume":"29","author":"Kurtul","year":"2015","journal-title":"Eye"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1016\/j.clae.2020.03.001","article-title":"Association between vitamin D and dry eye disease: A systematic review and meta-analysis of observational studies","volume":"43","author":"Askari","year":"2020","journal-title":"Contact Lens Anterior Eye"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1097\/ICO.0000000000001822","article-title":"Vitamin D Enhances the Efficacy of Topical Artificial Tears in Patients With Dry Eye Disease","volume":"38","author":"Hwang","year":"2019","journal-title":"Cornea"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2140","DOI":"10.1167\/iovs.13-13019","article-title":"Characterization of vitamin D production by human ocular barrier cells","volume":"55","author":"Alsalem","year":"2014","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"108668","DOI":"10.1016\/j.exer.2021.108668","article-title":"Topical calcitriol application promotes diabetic corneal wound healing and reinnervation through inhibiting NLRP3 inflammasome activation","volume":"209","author":"Wang","year":"2021","journal-title":"Exp. Eye Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3546","DOI":"10.1172\/JCI98093","article-title":"Optimal bone fracture repair requires 24R,25-dihydroxyvitamin D3 and its effector molecule FAM57B2","volume":"128","author":"Martineau","year":"2018","journal-title":"J. Clin. Investig."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1002\/jcb.20875","article-title":"Evidence that both 1alpha,25-dihydroxyvitamin D3 and 24-hydroxylated D3 enhance human osteoblast differentiation and mineralization","volume":"99","author":"Koedam","year":"2006","journal-title":"J. Cell Biochem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"871","DOI":"10.3109\/02713683.2012.688235","article-title":"Enhancement of vitamin D metabolites in the eye following vitamin D3 supplementation and UV-B irradiation","volume":"37","author":"Lin","year":"2012","journal-title":"Curr. Eye Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"16951","DOI":"10.1038\/s41598-017-16698-3","article-title":"Effects of 1,25 and 24,25 Vitamin D on Corneal Epithelial Proliferation, Migration and Vitamin D Metabolizing and Catabolizing Enzymes","volume":"7","author":"Lu","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2975","DOI":"10.1167\/iovs.13-13788","article-title":"Effects of vitamin D receptor knockout on cornea epithelium gap junctions","volume":"55","author":"Lu","year":"2014","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1080\/02713683.2021.1884726","article-title":"Effects of 1,25 and 24,25 Vitamin D on Corneal Fibroblast VDR and Vitamin D Metabolizing and Catabolizing Enzymes","volume":"46","author":"Lu","year":"2021","journal-title":"Curr. Eye Res."},{"key":"ref_46","first-page":"126","article-title":"Localization of Hla-a, Hla-B, Hla-C and Hla-Dr Antigens in the Human Cornea\u2014Practical Significance for Grafting Technique and Hla Typing","volume":"15","author":"Mayer","year":"1983","journal-title":"Transplant. Proc."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1097\/00007890-198503000-00011","article-title":"Histocompatibility antigen and passenger cell content of normal and diseased human cornea","volume":"39","author":"Williams","year":"1985","journal-title":"Transplantation"},{"key":"ref_48","first-page":"2264","article-title":"Identification of a novel macrophage population in the normal mouse corneal stroma","volume":"43","author":"Reynolds","year":"2002","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"7715","DOI":"10.1167\/iovs.15-17768","article-title":"Vitamin D Activation and Function in Human Corneal Epithelial Cells during TLR-Induced Inflammation","volume":"56","author":"Reins","year":"2015","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.exer.2014.01.004","article-title":"Integrin-dependent neutrophil migration in the injured mouse cornea","volume":"120","author":"Hanlon","year":"2014","journal-title":"Exp. Eye Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1034\/j.1600-065X.2000.17706.x","article-title":"The neutrophil as a cellular source of chemokines","volume":"177","author":"Scapini","year":"2000","journal-title":"Immunol. Rev."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1002\/emmm.201200221","article-title":"Identification of the HSPB4\/TLR2\/NF-kappaB axis in macrophage as a therapeutic target for sterile inflammation of the cornea","volume":"4","author":"Oh","year":"2012","journal-title":"EMBO Mol. Med."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1634\/stemcells.2004-0291","article-title":"CD34+ corneal stromal cells are bone marrow-derived and express hemopoietic stem cell markers","volume":"23","author":"Sosnova","year":"2005","journal-title":"Stem Cells"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"S71","DOI":"10.1097\/01.ico.0000178732.42921.05","article-title":"Bone marrow-derived cells in mouse and human cornea","volume":"24","author":"Yamagami","year":"2005","journal-title":"Cornea"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1568","DOI":"10.1167\/iovs.06-0746","article-title":"The chemokine receptor CX3CR1 mediates homing of MHC class II-positive cells to the normal mouse corneal epithelium","volume":"48","author":"Chinnery","year":"2007","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1089\/scd.2006.0084","article-title":"A novel population of repair cells identified in the stroma of the human cornea","volume":"16","author":"Thill","year":"2007","journal-title":"Stem Cells Dev."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"620284","DOI":"10.3389\/fimmu.2021.620284","article-title":"Resident Innate Immune Cells in the Cornea","volume":"12","author":"Liu","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"101105","DOI":"10.1016\/j.preteyeres.2022.101105","article-title":"Neuroimmune crosstalk in the cornea: The role of immune cells in corneal nerve maintenance during homeostasis and inflammation","volume":"91","author":"Wu","year":"2022","journal-title":"Prog. Retin. Eye Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1126\/science.6310748","article-title":"1,25-dihydroxyvitamin D3 receptors in human leukocytes","volume":"221","author":"Provvedini","year":"1983","journal-title":"Science"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1210\/jcem-57-6-1308","article-title":"Specific high-affinity receptors for 1,25-dihydroxyvitamin D3 in human peripheral blood mononuclear cells: Presence in monocytes and induction in T lymphocytes following activation","volume":"57","author":"Bhalla","year":"1983","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"106253","DOI":"10.1016\/j.jsbmb.2023.106253","article-title":"Two lineages of immune cells that differentially express the vitamin D receptor","volume":"228","author":"Cantorna","year":"2023","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2057","DOI":"10.1359\/jbmr.2001.16.11.2057","article-title":"In vitro and in vivo analysis of the immune system of vitamin D receptor knockout mice","volume":"16","author":"Mathieu","year":"2001","journal-title":"J. Bone Miner. Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1093\/intimm\/dxr045","article-title":"Converging pathways lead to overproduction of IL-17 in the absence of vitamin D signaling","volume":"23","author":"Bruce","year":"2011","journal-title":"Int. Immunol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"4983","DOI":"10.1038\/s41467-022-32636-y","article-title":"Diabetes downregulates the antimicrobial peptide psoriasin and increases E. coli burden in the urinary bladder","volume":"13","author":"Mohanty","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"633540","DOI":"10.3389\/fimmu.2021.633540","article-title":"Glucagon Reduces Neutrophil Migration and Increases Susceptibility to Sepsis in Diabetic Mice","volume":"12","author":"Insuela","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"271","DOI":"10.3389\/fendo.2017.00271","article-title":"Diabetes and Sepsis: Risk, Recurrence, and Ruination","volume":"8","author":"Frydrych","year":"2017","journal-title":"Front. Endocrinol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1063069","DOI":"10.3389\/fimmu.2023.1063069","article-title":"The association between altered intestinal microbiome, impaired systemic and ocular surface immunity, and impaired wound healing response after corneal alkaline-chemical injury in diabetic mice","volume":"14","author":"Bu","year":"2023","journal-title":"Front. Immunol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1007\/s11914-022-00732-z","article-title":"Vitamin D Regulation of Immune Function","volume":"20","author":"Bikle","year":"2022","journal-title":"Curr. Osteoporos. Rep."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"5880","DOI":"10.1167\/iovs.15-17177","article-title":"Vitamin D in Tear Fluid","volume":"56","author":"Lu","year":"2015","journal-title":"Investig. Ophthalmol. Vis. Sci."}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/13\/7\/1065\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:04:28Z","timestamp":1760126668000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/13\/7\/1065"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,1]]},"references-count":69,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2023,7]]}},"alternative-id":["biom13071065"],"URL":"https:\/\/doi.org\/10.3390\/biom13071065","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,1]]}}}