{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T17:31:31Z","timestamp":1769880691722,"version":"3.49.0"},"reference-count":26,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,8,10]],"date-time":"2023-08-10T00:00:00Z","timestamp":1691625600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Department of the Army, US Army Contracting Command, Aberdeen Proving Ground, Natick Contracting Division, Ft Detrick MD under a Deployed Warfighter Protection (DWFP) Program","award":["W911QY1910003"],"award-info":[{"award-number":["W911QY1910003"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Sand flies (Diptera: Psychodidae) serve as vectors for transmitting protozoan parasites, Leishmania spp., that cause the disease called leishmaniasis. The main approach to controlling sand flies is the use of chemical insecticides. The discovery of alternative methods for their control is needed because of potential health risks of chemical insecticides and development of sand fly resistance to these pesticides. The biomineral produced by diatoms (diatomaceous earth, DE; Celite) and a volcanic glass bio-mimic (Imergard) have been shown by our group to be efficacious against mosquitoes, filth flies, and ticks but never studied for the control of sand flies. In a modified World Health Organization cone test, 50% of adult Phlebotomus papatasi sand flies at 29 \u00b1 1 \u00b0C, 55 \u00b1 5% RH, and 12:12 LD, when exposed to Imergard and Celite, were dead in 13.08 and 7.57 h, respectively. Proof of concept was established for the use of these biominerals for sand fly and leishmaniasis disease control. Using a light source as an attractant to the minerals had no significant effect on the LT50, the time to 50% mortality. The LT50 at a higher relative humidity of 70 \u00b1 5% increased to 20.91 and 20.56 h for Imergard and Celite, respectively, suggesting their mode of action was dehydration. Scanning electron microscopy of dead sand flies showed high coating levels of Celite only on the sides of the thorax and on the tarsi, suggesting an alternative mode of action for mechanical insecticides.<\/jats:p>","DOI":"10.3390\/biom13081235","type":"journal-article","created":{"date-parts":[[2023,8,10]],"date-time":"2023-08-10T10:11:22Z","timestamp":1691662282000},"page":"1235","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Biomolecular Minerals and Volcanic Glass Bio-Mimics to Control Adult Sand Flies, the Vector of Human Leishmania Protozoan Parasites"],"prefix":"10.3390","volume":"13","author":[{"given":"Kaiying","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Entomology and Plant Pathology, North Carolina State University, 3230 Ligon Street, Raleigh, NC 27695, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean Marcel","family":"Deguenon","sequence":"additional","affiliation":[{"name":"Department of Entomology and Plant Pathology, North Carolina State University, 3230 Ligon Street, Raleigh, NC 27695, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger D.","family":"Lawrie","sequence":"additional","affiliation":[{"name":"Department of Entomology and Plant Pathology, North Carolina State University, 3230 Ligon Street, Raleigh, NC 27695, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6386-0994","authenticated-orcid":false,"given":"R. Michael","family":"Roe","sequence":"additional","affiliation":[{"name":"Department of Entomology and Plant Pathology, North Carolina State University, 3230 Ligon Street, Raleigh, NC 27695, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1146\/annurev-ento-120811-153557","article-title":"Biology of phlebotomine sand flies as vectors of disease agents","volume":"58","author":"Ready","year":"2013","journal-title":"Annu. Rev. Entomol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1016\/S0140-6736(05)67629-5","article-title":"Advances in leishmaniasis","volume":"366","author":"Murray","year":"2005","journal-title":"Lancet"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/S1473-3099(07)70209-8","article-title":"Cutaneous leishmaniasis","volume":"7","author":"Reithinger","year":"2007","journal-title":"Lancet Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"147","DOI":"10.2147\/CLEP.S44267","article-title":"Epidemiology of visceral leishmaniasis","volume":"6","author":"Ready","year":"2014","journal-title":"Clin. Epidemiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1586\/eri.10.19","article-title":"Current diagnosis and treatment of cutaneous and mucocutaneous leishmaniasis","volume":"8","author":"Goto","year":"2010","journal-title":"Expert Rev. Anti-Infect. Ther."},{"key":"ref_6","unstructured":"WHO (2023, July 28). Leishmaniasis. Available online: https:\/\/www.who.int\/health-topics\/leishmaniasis#tab=tab_1."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Wilson, A.L., Courtenay, O., Kelly-Hope, L.A., Scott, T.W., Takken, W., Torr, S.J., and Lindsay, S.W. (2020). The importance of vector control for the control and elimination of vector-borne diseases. PLoS Negl. Trop. Dis., 14.","DOI":"10.1371\/journal.pntd.0007831"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1046\/j.1365-2915.2003.00420.x","article-title":"Control of phlebotomine sandflies","volume":"17","author":"Alexander","year":"2003","journal-title":"Med. Vet. Entomol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.jspr.2005.03.001","article-title":"Toxic and behavioural effects of different modified diatomaceous earths on the German cockroach, Blattella germanica (L.) (Orthoptera: Blattellidae) under simulated field conditions","volume":"42","author":"Faulde","year":"2006","journal-title":"J. Stored Prod. Res."},{"key":"ref_10","unstructured":"Doggett, S.L., Geary, M.J., Lilly, D., and Russell, R.C. (2008). The Efficacy of Diatomaceous Earth against the Common Bed Bug, Cimex Lectularius, Department of Medical Entomology."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"101565","DOI":"10.1016\/j.jspr.2019.101565","article-title":"Evaluation of diatomaceous earth formulations enhanced with natural products against stored product insects","volume":"86","author":"Hamel","year":"2020","journal-title":"J. Stored Prod. Res."},{"key":"ref_12","first-page":"458","article-title":"New mosquitocide derived from volcanic rock","volume":"58","author":"Deguenon","year":"2020","journal-title":"J. Med. Entomol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Deguenon, J.M., Azondekon, R., Agossa, F.R., Padonou, G.G., Anagonou, R., Ahoga, J., N\u2019dombidje, B., Akinro, B., Stewart, D.A., and Wang, B. (2020). ImergardTM WP: A non-chemical alternative for an indoor residual spray, effective against pyrethroid-resistant Anopheles gambiae (sl) in Africa. Insects, 11.","DOI":"10.3390\/insects11050322"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1111\/mve.12521","article-title":"New thinking for filth fly control: Residual, non-chemical wall spray from volcanic glass","volume":"35","author":"Chen","year":"2021","journal-title":"Med. Vet. Entomol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Richardson, E.A., Ponnusamy, L., and Roe, R.M. (2022). Mechanical acaricides active against the blacklegged tick, Ixodes scapularis. Insects, 13.","DOI":"10.3390\/insects13080672"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"10","DOI":"10.18047\/poljo.22.1.2","article-title":"A review of natural insecticides based on diatomaceous earths","volume":"22","author":"Rozman","year":"2016","journal-title":"Poljoprivreda"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Luan, K., McCord, M.G., West, A.J., Cave, G., Travanty, N.V., Apperson, C.S., and Roe, R.M. (2023). Mosquito blood feeding prevention using an extra-low DC voltage charged cloth. Insects, 14.","DOI":"10.3390\/insects14050405"},{"key":"ref_18","unstructured":"WHO (1998). Test Procedures for Insecticide Resistance Monitoring in Malaria Vectors, Bio-Efficacy and Persistence of Insecticides on Treated Surfaces: Report of the WHO Informal Consultation, Geneva, 28\u201330 September 1998, World Health Organization."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1186\/s12936-022-04232-4","article-title":"A closer look at the WHO cone bioassay: Video analysis of the hidden effects of a human host on mosquito behaviour and insecticide contact","volume":"21","author":"Hughes","year":"2022","journal-title":"Malar. J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1603\/0022-2585-39.1.12","article-title":"Sand flies (Diptera: Phlebotominae) in Sanliurfa, Turkey: Relationship of Phlebotomus sergenti with the epidemic of anthroponotic cutaneous leishmaniasis","volume":"39","author":"Volf","year":"2002","journal-title":"J. Med. Entomol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1093\/jee\/18.2.265a","article-title":"A method of computing the effectiveness of an insecticide","volume":"18","author":"Abbott","year":"1925","journal-title":"J. Econ. Entom."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/S0022-474X(97)00039-8","article-title":"ReviewDiatomaceous earths, a group of natural insecticides","volume":"34","author":"Korunic","year":"1998","journal-title":"J. Stored Prod. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"301","DOI":"10.14411\/fp.2007.039","article-title":"Glycolytic and chitinolytic activities of Phlebotomus papatasi (Diptera: Psychodidae) from diverse ecological habitats","volume":"54","author":"Jacobson","year":"2007","journal-title":"Folia Parasitol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1006\/jare.1999.0499","article-title":"Studies on the role of climatological factors in the distribution of phlebotomine sandflies (Diptera: Psychodidae) in semi-arid areas of Rajasthan, India","volume":"42","author":"Singh","year":"1999","journal-title":"J. Arid Environ."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.actatropica.2005.05.002","article-title":"Seasonal fluctuations of phlebotomine sand fly populations (Diptera: Psychodidae) in the urban area of Marrakech, Morocco","volume":"95","author":"Boussaa","year":"2005","journal-title":"Acta Trop."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Cave, G.L., Richardson, E.A., Chen, K., Watson, D.W., and Roe, R.M. (2023). Acaricidal biominerals and mode-of-action studies against adult blacklegged ticks, Ixodes scapularis. Microorganisms, 11.","DOI":"10.3390\/microorganisms11081906"}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/13\/8\/1235\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:30:11Z","timestamp":1760128211000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/13\/8\/1235"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,10]]},"references-count":26,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["biom13081235"],"URL":"https:\/\/doi.org\/10.3390\/biom13081235","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,10]]}}}