{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T09:48:12Z","timestamp":1768038492454,"version":"3.49.0"},"reference-count":40,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T00:00:00Z","timestamp":1697414400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Background: Acute pancreatitis (AP) is a leading cause of gastrointestinal hospital admissions, with up to 40% mortality in patients with moderate\u2013severe AP. Glycoprotein acetylation (GlycA) is measured as a nuclear magnetic resonance signal (NMR) of the post-translational modification of glycosylated acute-phase proteins released during inflammation. We aimed to investigate the role of GlycA as an inflammatory biomarker of AP. Methods: We prospectively enrolled 20 AP patients and 22 healthy controls and collected EDTA plasma samples at admission and discharge. NMR spectra were acquired from these samples using a 400 MHz Vantera\u00ae Clinical Analyzer, and GlycA concentrations were calculated (normal = 400 \u03bcmol\/L). The GlycA NMR signal, at 2.00 \u00b1 0.01 ppm in the NMR spectrum, is derived from the N-acetyl methyl group protons within the carbohydrate side chains of circulating glycoproteins such as \u03b11-acid glycoprotein, haptoglobin, \u03b11-antitrypsin, \u03b11-antichymotrypsin, and transferrin. GlycA levels were then compared between AP patients and controls, as well as within the AP group, based on etiology and severity. Results: Demographic comparisons were similar, except for a higher BMI in AP patients compared to healthy controls (29.9 vs. 24.8 kg\/m2; p &lt; 0.001). AP was mild in 10 patients, moderate in 7, and severe in 3. GlycA levels were higher in AP patients than healthy controls on admission (578 vs. 376 \u03bcmol\/L, p &lt; 0.001) and at discharge (655 vs. 376 \u03bcmol\/L, p &lt; 0.001). GlycA levels were significantly higher in patients with moderate\u2013severe AP than in those with mild AP at discharge (533 vs. 757 \u03bcmol\/L, p = 0.023) but not at admission. After adjusting for BMI, multivariable regression indicated that patients with GlycA levels &gt; 400 \u03bcmol\/L had significantly higher odds of having AP of any severity (OR = 6.88; 95% CI, 2.07\u201332.2; p = 0.004) and mild AP (OR = 6.12; 95% CI, 1.48\u201342.0; p = 0.025) than controls. Conclusion: Our pilot study highlights the use of GlycA as a novel diagnostic biomarker of inflammation in patients with AP. Our study shows that GlycA levels were significantly higher in hospitalized AP patients compared to healthy controls. Patients with moderate-to-severe AP had higher GlycA levels compared to patients with mild AP at the time of their hospital discharge, suggesting persistent inflammation in patients with severe disease.<\/jats:p>","DOI":"10.3390\/biom13101530","type":"journal-article","created":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T05:43:49Z","timestamp":1697435029000},"page":"1530","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["GlycA: Evaluation of a New Biomarker of Acute Pancreatitis"],"prefix":"10.3390","volume":"13","author":[{"given":"Ishani","family":"Shah","sequence":"first","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Yakah","sequence":"additional","affiliation":[{"name":"Institute of Human Nutrition, Columbia University Medical Center, New York, NY 10032, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Awais","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven D.","family":"Freedman","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenghui G.","family":"Jiang","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunil G.","family":"Sheth","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1001\/jama.2020.20317","article-title":"Acute Pancreatitis: A Review","volume":"325","author":"Mederos","year":"2021","journal-title":"Jama"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1016\/S0140-6736(20)31310-6","article-title":"Acute pancreatitis","volume":"396","author":"Boxhoorn","year":"2020","journal-title":"Lancet"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1097\/MCG.0000000000001030","article-title":"Incidence, Admission Rates, and Predictors, and Economic Burden of Adult Emergency Visits for Acute Pancreatitis: Data from the National Emergency Department Sample, 2006 to 2012","volume":"53","author":"Garg","year":"2019","journal-title":"J. Clin. Gastroenterol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1097\/MPA.0000000000001275","article-title":"Trends and Outcomes of Hospitalizations Related to Acute Pancreatitis: Epidemiology from 2001 to 2014 in the United States","volume":"48","author":"Gapp","year":"2019","journal-title":"Pancreas"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1136\/gutjnl-2017-314657","article-title":"Impact of characteristics of organ failure and infected necrosis on mortality in necrotising pancreatitis","volume":"68","author":"Schepers","year":"2019","journal-title":"Gut"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1136\/gutjnl-2012-302779","article-title":"Classification of acute pancreatitis\u20142012: Revision of the Atlanta classification and definitions by international consensus","volume":"62","author":"Banks","year":"2013","journal-title":"Gut"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1476","DOI":"10.1053\/j.gastro.2012.03.005","article-title":"Comparison of existing clinical scoring systems to predict persistent organ failure in patients with acute pancreatitis","volume":"142","author":"Mounzer","year":"2012","journal-title":"Gastroenterology"},{"key":"ref_8","first-page":"443","article-title":"Objective early identification of severe acute pancreatitis","volume":"61","author":"Ranson","year":"1974","journal-title":"Am. J. Gastroenterol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0140-6736(89)90381-4","article-title":"APACHE-II score for assessment and monitoring of acute pancreatitis","volume":"2","author":"Larvin","year":"1989","journal-title":"Lancet"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e28121","DOI":"10.1097\/MD.0000000000028121","article-title":"The BISAP score, NLR, CRP, or BUN: Which marker best predicts the outcome of acute pancreatitis?","volume":"100","author":"Dancu","year":"2021","journal-title":"Medicine"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.clinre.2018.11.002","article-title":"The early prognostic value of inflammatory markers in patients with acute pancreatitis","volume":"43","author":"Liu","year":"2019","journal-title":"Clin. Res. Hepatol. Gastroenterol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"482","DOI":"10.7326\/M16-0650","article-title":"Prediction Models of Mortality in Acute Pancreatitis in Adults: A Systematic Review","volume":"165","author":"Di","year":"2016","journal-title":"Ann. Intern. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1016\/j.pan.2013.07.063","article-title":"IAP\/APA evidence-based guidelines for the management of acute pancreatitis","volume":"13","author":"Besselink","year":"2013","journal-title":"Pancreatology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1038\/ajg.2013.218","article-title":"American College of Gastroenterology guideline: Management of acute pancreatitis","volume":"108","author":"Tenner","year":"2013","journal-title":"Am. J. Gastroenterol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1056\/NEJM199902113400607","article-title":"Acute-phase proteins and other systemic responses to inflammation","volume":"340","author":"Gabay","year":"1999","journal-title":"N. Engl. J. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1373\/clinchem.2014.232918","article-title":"GlycA: A Composite Nuclear Magnetic Resonance Biomarker of Systemic Inflammation","volume":"61","author":"Otvos","year":"2015","journal-title":"Clin. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1093\/clinchem\/hvac160","article-title":"The Inflammation Biomarker GlycA Reflects Plasma N-Glycan Branching","volume":"69","author":"Noel","year":"2023","journal-title":"Clin. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"17","DOI":"10.21037\/jlpm.2020.03.03","article-title":"GlycA: A New Biomarker for Systemic Inflammation and Cardiovascular Disease (CVD) Risk Assessment","volume":"5","author":"Ballout","year":"2020","journal-title":"J. Lab. Precis. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"100120","DOI":"10.1016\/j.ajpc.2020.100120","article-title":"GlycA measured by NMR spectroscopy is associated with disease activity and cardiovascular disease risk in chronic inflammatory diseases","volume":"4","author":"Mehta","year":"2020","journal-title":"Am. J. Prev. Cardiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1093\/ecco-jcc\/jjy162","article-title":"GlycA, a Nuclear Magnetic Resonance Spectroscopy Measure for Protein Glycosylation, is a Viable Biomarker for Disease Activity in IBD","volume":"13","author":"Dierckx","year":"2019","journal-title":"J. Crohn\u2019s Colitis"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1177\/0961203315617842","article-title":"GlycA, a novel marker of inflammation, is elevated in systemic lupus erythematosus","volume":"25","author":"Chung","year":"2016","journal-title":"Lupus"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.clinbiochem.2014.07.015","article-title":"NMR measurement of LDL particle number using the Vantera Clinical Analyzer","volume":"47","author":"Matyus","year":"2014","journal-title":"Clin. Biochem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"107","DOI":"10.3748\/wjg.v25.i1.107","article-title":"Usefulness of urinary trypsinogen-2 and trypsinogen activation peptide in acute pancreatitis: A multicenter study in Japan","volume":"25","author":"Yasuda","year":"2019","journal-title":"World J. Gastroenterol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1186\/s12967-022-03338-2","article-title":"New challenges for microRNAs in acute pancreatitis: Progress and treatment","volume":"20","author":"Zhou","year":"2022","journal-title":"J. Transl. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1053\/j.gastro.2023.01.013","article-title":"Circulating Mitochondrial DNA as a Diagnostic Biomarker for Predicting Disease Severity in Patients With Acute Pancreatitis","volume":"164","author":"Yakah","year":"2023","journal-title":"Gastroenterology"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1159\/000105497","article-title":"Serum proteomic patterns as a predictor of severity in acute pancreatitis","volume":"7","author":"Papachristou","year":"2007","journal-title":"Pancreatology"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Silva-Vaz, P., Abrantes, A.M., Castelo-Branco, M., Gouveia, A., Botelho, M.F., and Tralh\u00e3o, J.G. (2020). Multifactorial Scores and Biomarkers of Prognosis of Acute Pancreatitis: Applications to Research and Practice. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21010338"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1002\/prca.200900150","article-title":"Glycosylation of liver acute-phase proteins in pancreatic cancer and chronic pancreatitis","volume":"4","author":"Sarrats","year":"2010","journal-title":"Proteom. Clin. Appl."},{"key":"ref_29","first-page":"337","article-title":"Prospective study of the prognostic value of C reactive protein, alpha 1-antitrypsin and alpha 1-acid glycoprotein in acute pancreatitis","volume":"79","author":"Casafont","year":"1991","journal-title":"Rev. Esp. Enfermedades Dig."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.aca.2015.02.008","article-title":"Quantitative analysis of N-glycans from human alfa-acid-glycoprotein using stable isotope labeling and zwitterionic hydrophilic interaction capillary liquid chromatography electrospray mass spectrometry as tool for pancreatic disease diagnosis","volume":"866","author":"Figueras","year":"2015","journal-title":"Anal. Chim. Acta"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"195","DOI":"10.5505\/tjtes.2012.99075","article-title":"Alpha-1 protease inhibitor and antichymotrypsin levels in acute pancreatitis","volume":"18","author":"Kemik","year":"2012","journal-title":"Turk. J. Trauma Emerg. Surg."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1777","DOI":"10.1111\/j.1572-0241.2001.03870.x","article-title":"Usefulness of carbohydrate-deficient transferrin and trypsin activity in the diagnosis of acute alcoholic pancreatitis","volume":"96","author":"Aparicio","year":"2001","journal-title":"Am. J. Gastroenterol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Mucha, A., Zaczek, M., Kralisz, M., Gruszewska, E., Cylwik, B., Panasiuk, A., and Chrostek, L. (2022). The Serum Profile of Transferrin Isoforms in Pancreatitis. J. Clin. Med., 11.","DOI":"10.3390\/jcm11061638"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1016\/j.clinbiochem.2008.01.026","article-title":"Change in transferrin sialylation is a potential prognostic marker for severity of acute pancreatitis","volume":"41","author":"Gornik","year":"2008","journal-title":"Clin. Biochem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2111","DOI":"10.1002\/ccr3.2641","article-title":"Branch Duct\u2014IPMN and PanIN, in IgG4-Autoimmune pancreatitis: A case report","volume":"8","author":"Lazzarin","year":"2020","journal-title":"Clin. Case Rep."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Fischer, K., Kettunen, J., W\u00fcrtz, P., Haller, T., Havulinna, A.S., Kangas, A.J., Soininen, P., Esko, T., Tammesoo, M.L., and M\u00e4gi, R. (2014). Biomarker profiling by nuclear magnetic resonance spectroscopy for the prediction of all-cause mortality: An observational study of 17,345 persons. PLoS Med., 11.","DOI":"10.1371\/journal.pmed.1001606"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1161\/CIRCGENETICS.114.000216","article-title":"Quantitative serum nuclear magnetic resonance metabolomics in cardiovascular epidemiology and genetics","volume":"8","author":"Soininen","year":"2015","journal-title":"Circ. Cardiovasc. Genet."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e003822","DOI":"10.1161\/JAHA.116.003822","article-title":"Circulating N-Linked Glycoprotein Side-Chain Biomarker, Rosuvastatin Therapy, and Incident Cardiovascular Disease: An Analysis from the JUPITER Trial","volume":"5","author":"Akinkuolie","year":"2016","journal-title":"J. Am. Heart Assoc."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Connelly, M.A., Shimizu, C., Winegar, D.A., Shalaurova, I., Pourfarzib, R., Otvos, J.D., Kanegaye, J.T., Tremoulet, A.H., and Burns, J.C. (2016). Differences in GlycA and lipoprotein particle parameters may help distinguish acute kawasaki disease from other febrile illnesses in children. BMC Pediatr., 16.","DOI":"10.1186\/s12887-016-0688-5"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1033660","DOI":"10.3389\/fcvm.2022.1033660","article-title":"Changes in HDL cholesterol, particles, and function associate with pediatric COVID-19 severity","volume":"9","author":"Suslovic","year":"2022","journal-title":"Front. Cardiovasc. Med."}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/13\/10\/1530\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:07:40Z","timestamp":1760130460000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/13\/10\/1530"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,16]]},"references-count":40,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2023,10]]}},"alternative-id":["biom13101530"],"URL":"https:\/\/doi.org\/10.3390\/biom13101530","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,16]]}}}