{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T03:48:56Z","timestamp":1776311336365,"version":"3.50.1"},"reference-count":63,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T00:00:00Z","timestamp":1698451200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Institutes of Health, NIH","award":["1R01GM112490-08"],"award-info":[{"award-number":["1R01GM112490-08"]}]},{"name":"the National Institutes of Health, NIH","award":["1R01GM130091-05"],"award-info":[{"award-number":["1R01GM130091-05"]}]},{"name":"the National Institutes of Health, NIH","award":["D-0005"],"award-info":[{"award-number":["D-0005"]}]},{"name":"the National Institutes of Health, NIH","award":["2779779"],"award-info":[{"award-number":["2779779"]}]},{"name":"Robert A. Welch Foundation","award":["1R01GM112490-08"],"award-info":[{"award-number":["1R01GM112490-08"]}]},{"name":"Robert A. Welch Foundation","award":["1R01GM130091-05"],"award-info":[{"award-number":["1R01GM130091-05"]}]},{"name":"Robert A. Welch Foundation","award":["D-0005"],"award-info":[{"award-number":["D-0005"]}]},{"name":"Robert A. Welch Foundation","award":["2779779"],"award-info":[{"award-number":["2779779"]}]},{"name":"Italian Ministry of Health Ricerca Corrente","award":["1R01GM112490-08"],"award-info":[{"award-number":["1R01GM112490-08"]}]},{"name":"Italian Ministry of Health Ricerca Corrente","award":["1R01GM130091-05"],"award-info":[{"award-number":["1R01GM130091-05"]}]},{"name":"Italian Ministry of Health Ricerca Corrente","award":["D-0005"],"award-info":[{"award-number":["D-0005"]}]},{"name":"Italian Ministry of Health Ricerca Corrente","award":["2779779"],"award-info":[{"award-number":["2779779"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Glycoproteomic analysis is always challenging because of low abundance and complex site-specific heterogeneity. Glycoproteins are involved in various biological processes such as cell signaling, adhesion, and cell\u2013cell communication and may serve as potential biomarkers when analyzing different diseases. Here, we investigate glycoproteins in narcolepsy type 1 (NT1) disease, a form of narcolepsy characterized by cataplexy\u2014the sudden onset of muscle paralysis that is typically triggered by intense emotions. In this study, 27 human blood serum samples were analyzed, 16 from NT1 patients and 11 from healthy individuals serving as controls. We quantified hydrophilic interaction liquid chromatography (HILIC)-enriched glycopeptides from low-abundance serum samples of controls and NT1 patients via LC-MS\/MS. Twenty-eight unique N-glycopeptides showed significant changes between the two studied groups. The sialylated N-glycopeptide structures LPTQNITFQTESSVAEQEAEFQSPK HexNAc6, Hex3, Neu5Ac2 (derived from the ITIH4 protein) and the structure IVLDPSGSMNIYLVLDGSDSIGASNFTGAK HexNAc5, Hex4, Fuc1 (derived from the CFB protein), with p values of 0.008 and 0.01, respectively, were elevated in NT1 samples compared with controls. In addition, the N-glycopeptide protein sources Ceruloplasmin, Complement factor B, and ITH4 were observed to play an important role in the complement activation and acute-phase response signaling pathways. This may explain the possible association between the biomarkers and pathophysiological effects.<\/jats:p>","DOI":"10.3390\/biom13111589","type":"journal-article","created":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T09:27:28Z","timestamp":1698658048000},"page":"1589","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["LC-MS\/MS Quantitation of HILIC-Enriched N-glycopeptides Derived from Low-Abundance Serum Glycoproteins in Patients with Narcolepsy Type 1"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-9518-3833","authenticated-orcid":false,"given":"Mojgan","family":"Atashi","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5229-4723","authenticated-orcid":false,"given":"Cristian D. Gutierrez","family":"Reyes","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vishal","family":"Sandilya","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Waziha","family":"Purba","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0184-4224","authenticated-orcid":false,"given":"Parisa","family":"Ahmadi","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md. Abdul","family":"Hakim","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5008-6944","authenticated-orcid":false,"given":"Firas","family":"Kobeissy","sequence":"additional","affiliation":[{"name":"Department of biochemistry and molecular genetics, Faculty of Biochemistry and Molecular Genetics, American University of Beirut, Beirut 11072020, Lebanon"},{"name":"Department of Neurobiology, Center for Neurotrauma, Multiomics & Biomarkers (CNMB), Neuroscience Institute, Morehouse School of Medicine, Atlanta, GE 30310, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Plazzi","sequence":"additional","affiliation":[{"name":"IRCCS, Instituto delle Scienze Neurologiche di Bologna, 40124 Bologna, Italy"},{"name":"Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, 41125 Modena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica","family":"Moresco","sequence":"additional","affiliation":[{"name":"IRCCS, Instituto delle Scienze Neurologiche di Bologna, 40124 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bartolo","family":"Lanuzza","sequence":"additional","affiliation":[{"name":"Sleep Research Center, Department of Neurology IC, Oasi Research Institute-IRCCS, 94018 Tronia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6937-3065","authenticated-orcid":false,"given":"Raffaele","family":"Ferri","sequence":"additional","affiliation":[{"name":"Sleep Research Center, Department of Neurology IC, Oasi Research Institute-IRCCS, 94018 Tronia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6661-6073","authenticated-orcid":false,"given":"Yehia","family":"Mechref","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1387","DOI":"10.1378\/chest.14-0970","article-title":"International classification of sleep disorders-third edition: Highlights and modifications","volume":"146","author":"Sateia","year":"2014","journal-title":"Chest"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1002\/ijc.2910330119","article-title":"Monoclonal antibody against a cryptic carbohydrate antigen of murine and human lymphocytes. 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