{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T14:14:19Z","timestamp":1777299259835,"version":"3.51.4"},"reference-count":15,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T00:00:00Z","timestamp":1700697600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Amyotrophic lateral sclerosis (ALS) is the most common neurodegenerative motor neuron disease and remains misunderstood with a difficult diagnosis and prognosis. The implication of the immune system is recognized in ALS pathophysiology, hence the interest in leucocyte count as lymphocytes and neutrophils. The neutrophil-to-lymphocyte ratio (NLR) has recently been used as a prognosis factor to assess the progression of ALS. Thus, the aim of this study was to analyze the evolution of the NLR during disease evolution in a French cohort of ALS patients and its relation with survival. In this monocentric retrospective study, clinical parameters and NLR were collected in ALS patients followed at the University Hospital of Tours (France). ALS patients were subdivided into three groups regarding their NLR value at inclusion: group 1 (NLR &lt; 2); group 2 (NLR: 2\u20133); group 3 (NLR &gt; 3). A comparison of qualitative and quantitative clinical and biological variables between NLR groups was performed. Then, Cox regressions were carried out to determine the association of NLR with survival. We observed a significant correlation of NLR with ALSFRS-r score (p &lt; 0.0001) and with vital forced capacity (p = 0.0004) at inclusion. We observed that increased NLR at diagnosis is associated with decreased ALS patients\u2019 survival.<\/jats:p>","DOI":"10.3390\/biom13121689","type":"journal-article","created":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T03:45:18Z","timestamp":1700711118000},"page":"1689","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Neutrophil to Lymphocyte Ratio as a Prognostic Marker in Amyotrophic Lateral Sclerosis"],"prefix":"10.3390","volume":"13","author":[{"given":"Camille","family":"Cotet","sequence":"first","affiliation":[{"name":"Laboratoire de Biochimie et Biologie Mol\u00e9culaire, CHRU Bretonneau, 2 Boulevard Tonnell\u00e9, 37000 Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hugo","family":"Alarcan","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie et Biologie Mol\u00e9culaire, CHRU Bretonneau, 2 Boulevard Tonnell\u00e9, 37000 Tours, France"},{"name":"UMR 1253 iBrain, Universit\u00e9 de Tours, Inserm, 10 Boulevard Tonnell\u00e9, 37000 Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7419-1124","authenticated-orcid":false,"given":"Olivier","family":"H\u00e9rault","sequence":"additional","affiliation":[{"name":"Service d\u2019H\u00e9matologie Biologique, CHRU Bretonneau, 2 Boulevard Tonnell\u00e9, 37000 Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Corcia","sequence":"additional","affiliation":[{"name":"UMR 1253 iBrain, Universit\u00e9 de Tours, Inserm, 10 Boulevard Tonnell\u00e9, 37000 Tours, France"},{"name":"Service de Neurologie, CHRU Bretonneau, 2 Boulevard Tonnell\u00e9, 37000 Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Vourc\u2019h","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie et Biologie Mol\u00e9culaire, CHRU Bretonneau, 2 Boulevard Tonnell\u00e9, 37000 Tours, France"},{"name":"UMR 1253 iBrain, Universit\u00e9 de Tours, Inserm, 10 Boulevard Tonnell\u00e9, 37000 Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian R.","family":"Andres","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie et Biologie Mol\u00e9culaire, CHRU Bretonneau, 2 Boulevard Tonnell\u00e9, 37000 Tours, France"},{"name":"UMR 1253 iBrain, Universit\u00e9 de Tours, Inserm, 10 Boulevard Tonnell\u00e9, 37000 Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9l\u00e8ne","family":"Blasco","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie et Biologie Mol\u00e9culaire, CHRU Bretonneau, 2 Boulevard Tonnell\u00e9, 37000 Tours, France"},{"name":"UMR 1253 iBrain, Universit\u00e9 de Tours, Inserm, 10 Boulevard Tonnell\u00e9, 37000 Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4512-2303","authenticated-orcid":false,"given":"Charlotte","family":"Veyrat-Durebex","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie et Biologie Mol\u00e9culaire, CHRU Bretonneau, 2 Boulevard Tonnell\u00e9, 37000 Tours, France"},{"name":"UMR 1253 iBrain, Universit\u00e9 de Tours, Inserm, 10 Boulevard Tonnell\u00e9, 37000 Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e1466","DOI":"10.1212\/WNL.0000000000012632","article-title":"Prediagnostic Neurofilament Light Chain Levels in Amyotrophic Lateral Sclerosis","volume":"97","author":"Bjornevik","year":"2021","journal-title":"Neurology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1136\/jnnp-2018-318704","article-title":"Neurofilament Light Chain in Serum for the Diagnosis of Amyotrophic Lateral Sclerosis","volume":"90","author":"Verde","year":"2019","journal-title":"J. Neurol. Neurosurg. Psychiatry"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"17071","DOI":"10.1038\/nrdp.2017.71","article-title":"Amyotrophic Lateral Sclerosis","volume":"3","author":"Hardiman","year":"2017","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.jneuroim.2004.10.009","article-title":"Evidence for Systemic Immune System Alterations in Sporadic Amyotrophic Lateral Sclerosis (sALS)","volume":"159","author":"Zhang","year":"2005","journal-title":"J. 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Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"474","DOI":"10.4149\/BLL_2021_078","article-title":"Neutrophil-to-Lymphocyte Ratio, Past, Present and Future Perspectives","volume":"122","author":"Zahorec","year":"2021","journal-title":"BLL"}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/13\/12\/1689\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:27:52Z","timestamp":1760131672000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/13\/12\/1689"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,23]]},"references-count":15,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["biom13121689"],"URL":"https:\/\/doi.org\/10.3390\/biom13121689","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,23]]}}}