{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T12:01:13Z","timestamp":1792324873440,"version":"4.4.0"},"reference-count":60,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,12,9]],"date-time":"2023-12-09T00:00:00Z","timestamp":1702080000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Sciences and Engineering Research Council"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Fibrotic cataracts, posterior capsular opacification (PCO), and anterior subcapsular cataracts (ASC) are mainly attributed to the transforming growth factor-\u03b2 (TGF\u03b2)-induced epithelial-to-mesenchymal transition (EMT) of lens epithelial cells (LECs). Previous investigations from our laboratory have shown the novel role of non-canonical TGF\u03b2 signaling in the progression of EMT in LECs. In this study, we have identified YAP as a critical signaling molecule involved in lens fibrosis. The observed increase in nuclear YAP in capsules of human ASC patients points toward the involvement of YAP in lens fibrosis. In addition, the immunohistochemical (IHC) analyses on ocular sections from mice that overexpress TGF\u03b2 in the lens (TGF\u03b2tg) showed a co-expression of YAP and \u03b1-SMA in the fibrotic plaques when compared to wild-type littermate lenses, which do not. The incubation of rat lens explants with verteporfin, a YAP inhibitor, prevented a TGF\u03b2-induced fiber-like phenotype, \u03b1-SMA, and fibronectin expression, as well as delocalization of E-cadherin and \u03b2-catenin. Finally, LECs co-incubated with TGF\u03b2 and YAP inhibitor did not exhibit an induction in matrix metalloproteinase 2 compared to those LECs treated with TGF\u03b2 alone. In conclusion, these data demonstrate that YAP is required for TGF\u03b2-mediated lens EMT and fibrosis.<\/jats:p>","DOI":"10.3390\/biom13121767","type":"journal-article","created":{"date-parts":[[2023,12,11]],"date-time":"2023-12-11T06:56:00Z","timestamp":1702277760000},"page":"1767","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Understanding the Role of Yes-Associated Protein (YAP) Signaling in the Transformation of Lens Epithelial Cells (EMT) and Fibrosis"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5546-9928","authenticated-orcid":false,"given":"Aftab","family":"Taiyab","sequence":"first","affiliation":[{"name":"Department of Pathology and Molecular Medicine, Faculty of Health Sciences, McMaster University, Hamilton, ON L8N 3Z5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasmine","family":"Belahlou","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine, Faculty of Health Sciences, McMaster University, Hamilton, ON L8N 3Z5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine, Faculty of Health Sciences, McMaster University, Hamilton, ON L8N 3Z5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saranya","family":"Pandi","sequence":"additional","affiliation":[{"name":"Department of Immunology and Stem Cell Biology, Aravind Medical Research Foundation, Madurai 625020, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8009-7399","authenticated-orcid":false,"given":"Madhu","family":"Shekhar","sequence":"additional","affiliation":[{"name":"Cataract and IOL Services, Aravind Eye Hospital and Post Graduate Institute of Ophthalmology, Madurai 625020, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5070-8547","authenticated-orcid":false,"given":"Gowri Priya","family":"Chidambaranathan","sequence":"additional","affiliation":[{"name":"Department of Immunology and Stem Cell Biology, Aravind Medical Research Foundation, Madurai 625020, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4293-9131","authenticated-orcid":false,"given":"Judith","family":"West-Mays","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine, Faculty of Health Sciences, McMaster University, Hamilton, ON L8N 3Z5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,9]]},"reference":[{"key":"ref_1","first-page":"2696","article-title":"Human age-related cataract and lens epithelial cell death","volume":"39","author":"Harocopos","year":"1998","journal-title":"Investig. 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