{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T19:57:22Z","timestamp":1773950242515,"version":"3.50.1"},"reference-count":47,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T00:00:00Z","timestamp":1705968000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"German Federal Ministries of Education and Research (BMBF)","award":["IP7\/01KI2007D"],"award-info":[{"award-number":["IP7\/01KI2007D"]}]},{"name":"German Federal Ministries of Education and Research (BMBF)","award":["ZIM"],"award-info":[{"award-number":["ZIM"]}]},{"name":"German Federal Ministries of Education and Research (BMBF)","award":["ZF4117908 AJ8"],"award-info":[{"award-number":["ZF4117908 AJ8"]}]},{"name":"Federal Ministry for Economic Affairs and Energy","award":["IP7\/01KI2007D"],"award-info":[{"award-number":["IP7\/01KI2007D"]}]},{"name":"Federal Ministry for Economic Affairs and Energy","award":["ZIM"],"award-info":[{"award-number":["ZIM"]}]},{"name":"Federal Ministry for Economic Affairs and Energy","award":["ZF4117908 AJ8"],"award-info":[{"award-number":["ZF4117908 AJ8"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>The incidence of human Campylobacter jejuni infections is increasing worldwide. It is highly desirable to prevent campylobacteriosis in individuals at risk for severe disease with antibiotics-independent non-toxic compounds. Activated charcoal (AC) has long been used as an anti-diarrheal remedy. Here, we tested the disease-mitigating effects of oral AC versus placebo in human gut microbiota-associated (hma) IL-10\u2212\/\u2212 mice starting a week prior to C. jejuni infection. On day 6 post-infection, the gastrointestinal C. jejuni loads were comparable in both infected cohorts, whereas campylobacteriosis symptoms such as wasting and bloody diarrhea were mitigated upon AC prophylaxis. Furthermore, AC application resulted in less pronounced C. jejuni-induced colonic epithelial cell apoptosis and in dampened innate and adaptive immune cell responses in the colon that were accompanied by basal concentrations of pro-inflammatory mediators including IL-6, TNF-\u03b1, IFN-\u03b3, and nitric oxide measured in colonic explants from AC treated mice on day 6 post-infection. Furthermore, C. jejuni infection resulted in distinct fecal microbiota shift towards higher enterobacterial numbers and lower loads of obligate anaerobic species in hma mice that were AC-independent. In conclusion, our pre-clinical placebo-controlled intervention study provides evidence that prophylactic oral AC application mitigates acute murine campylobacteriosis.<\/jats:p>","DOI":"10.3390\/biom14020141","type":"journal-article","created":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T04:17:40Z","timestamp":1705983460000},"page":"141","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Prophylactic Oral Application of Activated Charcoal Mitigates Acute Campylobacteriosis in Human Gut Microbiota-Associated IL-10\u2212\/\u2212 Mice"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6399-651X","authenticated-orcid":false,"given":"Markus M.","family":"Heimesaat","sequence":"first","affiliation":[{"name":"Gastrointestinal Microbiology Research Group, Institute of Microbiology, Infectious Diseases and Immunology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of Health, 12203 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niklas","family":"Schabbel","sequence":"additional","affiliation":[{"name":"Gastrointestinal Microbiology Research Group, Institute of Microbiology, Infectious Diseases and Immunology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of Health, 12203 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Q.","family":"Langfeld","sequence":"additional","affiliation":[{"name":"Gastrointestinal Microbiology Research Group, Institute of Microbiology, Infectious Diseases and Immunology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of Health, 12203 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nizar W.","family":"Shayya","sequence":"additional","affiliation":[{"name":"Gastrointestinal Microbiology Research Group, Institute of Microbiology, Infectious Diseases and Immunology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of Health, 12203 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soraya","family":"Mousavi","sequence":"additional","affiliation":[{"name":"Gastrointestinal Microbiology Research Group, Institute of Microbiology, Infectious Diseases and Immunology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of Health, 12203 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Bereswill","sequence":"additional","affiliation":[{"name":"Gastrointestinal Microbiology Research Group, Institute of Microbiology, Infectious Diseases and Immunology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of Health, 12203 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,23]]},"reference":[{"key":"ref_1","unstructured":"WHO (2024, January 09). 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