{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T23:10:33Z","timestamp":1791933033291,"version":"4.3.4"},"reference-count":46,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T00:00:00Z","timestamp":1715731200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32172154"],"award-info":[{"award-number":["32172154"]}]},{"name":"National Natural Science Foundation of China","award":["LYG06521202107"],"award-info":[{"award-number":["LYG06521202107"]}]},{"name":"521 Program of Lianyungang","award":["32172154"],"award-info":[{"award-number":["32172154"]}]},{"name":"521 Program of Lianyungang","award":["LYG06521202107"],"award-info":[{"award-number":["LYG06521202107"]}]},{"name":"The Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)","award":["32172154"],"award-info":[{"award-number":["32172154"]}]},{"name":"The Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)","award":["LYG06521202107"],"award-info":[{"award-number":["LYG06521202107"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Peptides possessing antihypertensive attributes via inhibiting the angiotensin-converting enzyme (ACE) were derived through the enzymatic degradation of Trichiurus lepturus (ribbonfish) using alkaline protease. The resulting mixture underwent filtration using centrifugation, ultrafiltration tubes, and Sephadex G-25 gels. Peptides exhibiting ACE-inhibitory properties and DPPH free-radical-scavenging abilities were isolated and subsequently purified via LC\/MS-MS, leading to the identification of over 100 peptide components. In silico screening yielded five ACE inhibitory peptides: FAGDDAPR, QGPIGPR, IFPRNPP, AGFAGDDAPR, and GPTGPAGPR. Among these, IFPRNPP and AGFAGDDAPR were found to be allergenic, while FAGDDAPRR, QGPIGPR, and GPTGPAGP showed good ACE-inhibitory effects. IC50 values for the latter peptides were obtained from HUVEC cells: FAGDDAPRR (IC50 = 262.98 \u03bcM), QGPIGPR (IC50 = 81.09 \u03bcM), and GPTGPAGP (IC50 = 168.11 \u03bcM). Peptide constituents derived from ribbonfish proteins effectively modulated ACE activity, thus underscoring their therapeutic potential. Molecular docking and modeling corroborated these findings, emphasizing the utility of functional foods as a promising avenue for the treatment and prevention of hypertension, with potential ancillary health benefits and applications as substitutes for synthetic drugs.<\/jats:p>","DOI":"10.3390\/biom14050581","type":"journal-article","created":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T03:35:55Z","timestamp":1715744155000},"page":"581","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Novel Angiotensin-Converting Enzyme-Inhibitory Peptides Obtained from Trichiurus lepturus: Preparation, Identification and Potential Antihypertensive Mechanism"],"prefix":"10.3390","volume":"14","author":[{"given":"Jiaming","family":"Cao","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Marine Bioresources and Environment\/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China"},{"name":"Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boyuan","family":"Xiang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Marine Bioresources and Environment\/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China"},{"name":"Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3006-6978","authenticated-orcid":false,"given":"Baojie","family":"Dou","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Marine Bioresources and Environment\/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China"},{"name":"Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5381-8509","authenticated-orcid":false,"given":"Jingfei","family":"Hu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Marine Bioresources and Environment\/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China"},{"name":"Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Marine Bioresources and Environment\/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China"},{"name":"Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxin","family":"Kang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Marine Bioresources and Environment\/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China"},{"name":"Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4409-3766","authenticated-orcid":false,"given":"Mingsheng","family":"Lyu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Marine Bioresources and Environment\/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China"},{"name":"Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujun","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Marine Bioresources and Environment\/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China"},{"name":"Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Li, X., Feng, C., Hong, H., Zhang, Y., Luo, Z., Wang, Q., Luo, Y., and Tan, Y. 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