{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T10:39:42Z","timestamp":1790505582627,"version":"4.1.0"},"reference-count":79,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T00:00:00Z","timestamp":1720483200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Russian Science Foundation","award":["19-74-10056"],"award-info":[{"award-number":["19-74-10056"]}]},{"name":"the Russian Science Foundation","award":["21-64-00017"],"award-info":[{"award-number":["21-64-00017"]}]},{"name":"the Russian Science Foundation","award":["121031300041-4"],"award-info":[{"award-number":["121031300041-4"]}]},{"name":"Ministry of Science and Higher Education of Russian Federation","award":["19-74-10056"],"award-info":[{"award-number":["19-74-10056"]}]},{"name":"Ministry of Science and Higher Education of Russian Federation","award":["21-64-00017"],"award-info":[{"award-number":["21-64-00017"]}]},{"name":"Ministry of Science and Higher Education of Russian Federation","award":["121031300041-4"],"award-info":[{"award-number":["121031300041-4"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Nucleotide excision repair (NER) is the most universal repair pathway, which removes a wide range of DNA helix-distorting lesions caused by chemical or physical agents. The final steps of this repair process are gap-filling repair synthesis and subsequent ligation. XPA is the central NER scaffolding protein factor and can be involved in post-incision NER stages. Replication machinery is loaded after the first incision of the damaged strand that is performed by the XPF\u2013ERCC1 nuclease forming a damaged 5\u2032-flap processed by the XPG endonuclease. Flap endonuclease I (FEN1) is a critical component of replication machinery and is absolutely indispensable for the maturation of newly synthesized strands. FEN1 also contributes to the long-patch pathway of base excision repair. Here, we use a set of DNA substrates containing a fluorescently labeled 5\u2032-flap and different size gap to analyze possible repair factor\u2013replication factor interactions. Ternary XPA\u2013FEN1\u2013DNA complexes with each tested DNA are detected. Furthermore, we demonstrate XPA\u2013FEN1 complex formation in the absence of DNA due to protein\u2013protein interaction. Functional assays reveal that XPA moderately inhibits FEN1 catalytic activity. Using fluorescently labeled XPA, formation of ternary RPA\u2013XPA\u2013FEN1 complex, where XPA accommodates FEN1 and RPA contacts simultaneously, can be proposed. We discuss possible functional roles of the XPA\u2013FEN1 interaction in NER related DNA resynthesis and\/or other DNA metabolic processes where XPA can be involved in the complex with FEN1.<\/jats:p>","DOI":"10.3390\/biom14070814","type":"journal-article","created":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T11:17:09Z","timestamp":1720523829000},"page":"814","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Does the XPA\u2013FEN1 Interaction Concern to Nucleotide Excision Repair or Beyond?"],"prefix":"10.3390","volume":"14","author":[{"given":"Yuliya S.","family":"Krasikova","sequence":"first","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6800-5989","authenticated-orcid":false,"given":"Ekaterina A.","family":"Maltseva","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana N.","family":"Khodyreva","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey N.","family":"Evdokimov","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7496-7527","authenticated-orcid":false,"given":"Nadejda I.","family":"Rechkunova","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5980-8889","authenticated-orcid":false,"given":"Olga I.","family":"Lavrik","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine, 630090 Novosibirsk, Russia"},{"name":"Department of Natural Sciences, Novosibirsk State University, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1038\/emboj.2009.49","article-title":"Coordination of dual incision and repair synthesis in human nucleotide excision repair","volume":"28","author":"Staresincic","year":"2009","journal-title":"EMBO J."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Sch\u00e4rer, O.D. 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