{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T01:35:12Z","timestamp":1790127312370,"version":"4.0.1"},"reference-count":141,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T00:00:00Z","timestamp":1722297600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Human papillomavirus (HPV)-associated head and neck squamous cell carcinoma (HNSCC), particularly oropharyngeal squamous cell carcinoma (OPSCC), is an increasingly prevalent pathology worldwide, especially in developed countries. For diagnosing HPV in HNSCC, the combination of p16 immunohistochemistry (IHC) and polymerase chain reaction (PCR) offers high sensitivity and specificity, with p16 IHC being a reliable initial screen and PCR confirming HPV presence. Advanced techniques like next-generation sequencing (NGS) and RNA-based assays provide detailed insights but are primarily used in research settings. Regardless of HPV status, standard oncological treatments currently include surgery, radiation, and\/or chemotherapy. This conventional approach does not account for the typically better prognosis of HPV-positive HNSCC patients, leading to increased chemo\/radiation-induced secondary morbidities and reduced quality of life. Therefore, it is crucial to identify and detect HPV positivity and other molecular characteristics of HNSCC to personalize treatment strategies. This comprehensive review aims to summarize current knowledge on various HPV detection techniques and evaluate their advantages and disadvantages, with a focus on developing methodologies to identify new biomarkers in HPV-positive HNSCC. The review discusses direct and indirect HPV examination in tumor tissue, DNA- and RNA-based detection techniques, protein-based markers, liquid biopsy potentials, immune-related markers, epigenetic markers, novel biomarkers, and emerging technologies, providing an overall insight into the current state of knowledge.<\/jats:p>","DOI":"10.3390\/biom14080925","type":"journal-article","created":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T12:38:20Z","timestamp":1722515900000},"page":"925","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["The Next Chapter in Cancer Diagnostics: Advances in HPV-Positive Head and Neck Cancer"],"prefix":"10.3390","volume":"14","author":[{"given":"Antea","family":"Krsek","sequence":"first","affiliation":[{"name":"Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2837-4157","authenticated-orcid":false,"given":"Lara","family":"Baticic","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Braut","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology and Head and Neck Surgery, Clinical Hospital Centre Rijeka, 51000 Rijeka, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2645-5364","authenticated-orcid":false,"given":"Vlatka","family":"Sotosek","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Reanimatology, Emergency and Intensive Care Medicine, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia"},{"name":"Department of Clinical Medical Sciences I, Faculty of Health Studies, University of Rijeka, 51000 Rijeka, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Khoo, A., Boyer, M., Jafri, Z., Makeham, T., Pham, T., Khachigian, L.M., Floros, P., Dowling, E., Fedder, K., and Shonka, D. (2024). Human Papilloma Virus Positive Oropharyngeal Squamous Cell Carcinoma and the Immune System: Pathogenesis, Immunotherapy and Future Perspectives. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25052798"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"51","DOI":"10.3322\/caac.21384","article-title":"The global incidence of lip, oral cavity, and pharyngeal cancers by subsite in 2012","volume":"67","author":"Shield","year":"2017","journal-title":"CA Cancer J. Clin."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"5489","DOI":"10.1007\/s00405-023-08115-5","article-title":"A comparison between p16-positive head and neck cancer of unknown primary (HPV-HNCUP) and oropharyngeal squamous cell carcinoma (HPV-OPSCC): Are they the same disease?","volume":"280","author":"Balk","year":"2023","journal-title":"Eur. Arch. Otorhinolaryngol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1158\/1940-6207.CAPR-19-0098","article-title":"Increasing incidence rates of oropharyngeal squamous cell carcinoma in Germany and significance of disease burden attributed to human papillomavirus","volume":"12","author":"Wittekindt","year":"2019","journal-title":"Cancer Prev. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.ejca.2020.04.027","article-title":"The current epidemic of HPV-associated oropharyngeal cancer: An 18-year Danish population-based study with 2169 patients","volume":"134","author":"Zamani","year":"2020","journal-title":"Eur. J. Cancer"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e180","DOI":"10.1016\/S2214-109X(19)30488-7","article-title":"Global burden of cancer attributable to infections in 2018: A worldwide incidence analysis","volume":"8","author":"Georges","year":"2020","journal-title":"Lancet Glob. Health"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/0007-117X(83)90060-4","article-title":"Immunohistochemical demonstration of Human papilloma virus (HPV) antigens in oral squamous cell lesions","volume":"21","author":"Lamberg","year":"1983","journal-title":"Br. J. Oral Surg."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e15568","DOI":"10.7717\/peerj.15568","article-title":"The relation between human papillomavirus (HPV) and oropharyngeal cancer: A review","volume":"11","author":"Ferreira","year":"2023","journal-title":"PeerJ"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Welters, M.J.P., Santegoets, S.J., and van der Burg, S.H. (2020). The Tumor Microenvironment and Immunotherapy of Oropharyngeal Squamous Cell Carcinoma. Front. Oncol., 10.","DOI":"10.3389\/fonc.2020.545385"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Yarbrough, W.G., Schrank, T.P., Burtness, B.A., and Issaeva, N. (2024). De-Escalated Therapy and Early Treatment of Recurrences in HPV-Associated Head and Neck Cancer: The Potential for Biomarkers to Revolutionize Personalized Therapy. Viruses, 16.","DOI":"10.3390\/v16040536"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1001\/jama.2012.101","article-title":"Prevalence of oral HPV infection in the United States, 2009\u20132010","volume":"307","author":"Gillison","year":"2012","journal-title":"JAMA"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1093\/annonc\/mdr006","article-title":"Survival and human papillomavirus in oropharynx cancer in TAX 324: A subset analysis from an international phase III trial","volume":"22","author":"Posner","year":"2011","journal-title":"Ann. Oncol."},{"key":"ref_13","first-page":"17","article-title":"Head and neck tumours","volume":"8","year":"2017","journal-title":"UICC TNM Classif. Malig. Tumours"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1200\/JCO.2010.31.8311","article-title":"Second primary cancers after an index head and neck cancer: Subsite-specific trends in the era of human papillomavirus-associated oropharyngeal cancer","volume":"29","author":"Morris","year":"2011","journal-title":"J. Clin. Oncol."},{"key":"ref_15","first-page":"281","article-title":"De-Escalation Treatment for Human Papillomavirus\u2013Related Oropharyngeal Cancer: Questions for Practical Consideration","volume":"37","author":"Chen","year":"2023","journal-title":"Oncology"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3668","DOI":"10.3390\/curroncol29050295","article-title":"De-Escalation Strategies for Human Papillomavirus-Associated Oropharyngeal Squamous Cell Carcinoma\u2014Where Are We Now?","volume":"29","author":"Silver","year":"2022","journal-title":"Curr. Oncol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Elhalawani, H., Mohamed, A.S.R., Elgohari, B., Lin, T.A., Sikora, A.G., Lai, S.Y., Abusaif, A., Phan, J., Morrison, W.H., and Gunn, G.B. (2020). Tobacco exposure as a major modifier of oncologic outcomes in human papillomavirus (HPV) associated oropharyngeal squamous cell carcinoma. BMC Cancer, 20.","DOI":"10.1186\/s12885-020-07427-7"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Lai, Y.H., Su, C.C., Wu, S.Y., Hsueh, W.T., Wu, Y.H., Chen, H.H.W., Hsiao, J.R., Liu, C.H., and Tsai, Y.S. (2022). Impact of Alcohol and Smoking on Outcomes of HPV-Related Oropharyngeal Cancer. J. Clin. Med., 11.","DOI":"10.3390\/jcm11216510"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"S63","DOI":"10.1007\/s12105-012-0372-5","article-title":"HPV detection methods in head and neck cancer","volume":"6","author":"Venuti","year":"2012","journal-title":"Head Neck Pathol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3292","DOI":"10.1128\/JCM.00539-06","article-title":"Highly effective detection of human papillomavirus 16 and 18 DNA by a testing algorithm combining broad-spectrum and type-specific PCR","volume":"44","author":"Molijn","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1099\/0022-1317-72-1-173","article-title":"Human papillomavirus DNA in biopsies of oral tissues","volume":"72","author":"Yeudall","year":"1991","journal-title":"J. Gen. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1007\/978-1-60761-947-5_4","article-title":"In situ detection of human papillomavirus DNA after PCR-amplification","volume":"688","author":"Nuovo","year":"2011","journal-title":"Methods Mol. Biol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.3389\/fonc.2020.01751","article-title":"HPV Detection in Head and Neck Squamous Cell Carcinomas: What Is the Issue?","volume":"10","author":"Augustin","year":"2020","journal-title":"Front. Oncol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"5463","DOI":"10.1073\/pnas.74.12.5463","article-title":"DNA sequencing with chain-terminating inhibitors","volume":"74","author":"Sanger","year":"1977","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1073\/pnas.74.2.560","article-title":"A new method for sequencing DNA","volume":"74","author":"Maxam","year":"1977","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Kim, S., Lee, J.W., and Park, Y.-S. (2020). The Application of Next-Generation Sequencing to Define Factors Related to Oral Cancer and Discover Novel Biomarkers. Life, 10.","DOI":"10.3390\/life10100228"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1200\/PO.18.00169","article-title":"Use of next-generation sequencing tests to guide cancer treatment: Results from a nationally representative survey of oncologists in the United States","volume":"2","author":"Freedman","year":"2018","journal-title":"JCO Precis. Oncol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"100487","DOI":"10.1016\/j.eclinm.2020.100487","article-title":"When should we order a next generation sequencing test in a patient with cancer?","volume":"25","author":"Colomer","year":"2020","journal-title":"eClinicalMedicine"},{"key":"ref_29","unstructured":"(2024, June 08). List of Cleared or Approved Companion Diagnostic Devices (In Vitro and Imaging Tools), Available online: https:\/\/www.fda.gov\/medical-devices\/in-vitro-diagnostics\/list-cleared-or-approved-companion-diagnostic-devices-in-vitro-and-imaging-tools."},{"key":"ref_30","unstructured":"Bi\u00e8che, I., Kamal, M., and Tourneau, C.L. (2017). Multigene Sequencing for Treatment Selection: Esmo Biomarker Factsheet, Oncology Pro. Available online: https:\/\/oncologypro.esmo.org\/education-library\/factsheets-on-biomarkers."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"288","DOI":"10.5455\/medarh.2017.71.288-292","article-title":"Overview of Next-generation Sequencing Platforms Used in Published Draft Plant Genomes in Light of Genotypization of Immortelle Plant (Helichrysium arenarium)","volume":"71","author":"Hodzic","year":"2017","journal-title":"Med. Arch."},{"key":"ref_32","first-page":"1","article-title":"Comparison of Next-Generation Sequencing Systems","volume":"2012","author":"Liu","year":"2012","journal-title":"J. Biomed. Biotechnol."},{"key":"ref_33","unstructured":"(2019). Oxford Nanopore bests PacBio. Nat. Biotechnol., 37."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1186\/s13007-020-00629-x","article-title":"Analysis and comprehensive comparison of PacBio and nanopore-based RNA sequencing of the Arabidopsis transcriptome","volume":"16","author":"Cui","year":"2020","journal-title":"Plant Methods"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Lechner, A., Kumbrink, J., Walz, C., Jung, A., Baumeister, P., and Flach, S. (2024). Molecular characterization of the evolution of premalignant lesions in the upper aerodigestive tract. Front. Oncol., 14.","DOI":"10.3389\/fonc.2024.1364958"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1093\/annonc\/mdv109","article-title":"Genomic alterations in head and neck squamous cell carcinoma determined by cancer gene-targeted sequencing","volume":"26","author":"Chung","year":"2015","journal-title":"Ann. Oncol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"032023","DOI":"10.1088\/1755-1315\/332\/3\/032023","article-title":"A comparative study of ddPCR and sanger sequencing for quantitative detection of low-frequency mutation rate","volume":"332","author":"Zhao","year":"2019","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1309\/AJCPMWGWGO34EGOD","article-title":"Clinical Validation of KRAS, BRAF, and EGFR Mutation Detection Using Next-Generation Sequencing","volume":"141","author":"Lin","year":"2014","journal-title":"Am. J. Clin. Pathol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1016\/j.joms.2018.09.014","article-title":"Intratumoral Heterogeneity in Recurrent Metastatic Squamous Cell Carcinoma of the Oral Cavity: New Perspectives Afforded by Multiregion DNA Sequencing and mtDNA Analysis","volume":"77","author":"Gabusi","year":"2020","journal-title":"J. Oral Maxillofac. Surg."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1007\/s00428-019-02656-z","article-title":"Prognostic impact of intra-field heterogeneity in oral squamous cell carcinoma","volume":"476","author":"Gabusi","year":"2019","journal-title":"Virchows Arch."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2939","DOI":"10.1158\/1078-0432.CCR-15-2314","article-title":"Incorporation of next-generation sequencing into routine clinical care to direct treatment of head and neck squamous cell carcinoma","volume":"22","author":"Chau","year":"2016","journal-title":"Clin. Cancer Res."},{"key":"ref_42","first-page":"491","article-title":"The Third Component of Complement: New Functions for an Old Friend","volume":"122","author":"Hostetter","year":"1993","journal-title":"J. Lab. Clin. Med."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Ludwig, S., Sharma, P., Wise, P., Sposto, R., Hollingshead, D., Lamb, J., Lang, S., Fabbri, M., and Whiteside, T.L. (2020). mRNA and miRNA Profiles of Exosomes from Cultured Tumor Cells Reveal Biomarkers Specific for HPV16-Positive and HPV16-Negative Head and Neck Cancer. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21228570"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1158\/1078-0432.CCR-18-0776","article-title":"A Five-MicroRNA Signature Predicts Survival and Disease Control of Patients with Head and Neck Cancer Negative for HPV Infection","volume":"25","author":"Hess","year":"2019","journal-title":"Clin. Cancer Res."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Hess, J., Unger, K., Maihoefer, C., Sch\u00fcttrumpf, L., Weber, P., Marschner, S., Wintergerst, L., Pflugradt, U., Baumeister, P., and Walch, A. (2022). Integration of P16\/HPV DNA Status with a 24-miRNA-Defined Molecular Phenotype Improves Clinically Relevant Stratification of Head and Neck Cancer Patients. Cancers, 14.","DOI":"10.3390\/cancers14153745"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.ejca.2017.02.018","article-title":"MiR-200b and miR-155 as Predictive Biomarkers for the Efficacy of Chemoradiation in Locally Advanced Head and Neck Squamous Cell Carcinoma","volume":"77","author":"Hess","year":"2017","journal-title":"Eur. J. Cancer"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"106417","DOI":"10.1016\/j.oraloncology.2023.106417","article-title":"Relationship of HPV16 E6 Seropositivity with Circulating Tumor Tissue Modified HPV16 DNA before Head and Neck Cancer Diagnosis","volume":"141","author":"Rettig","year":"2023","journal-title":"Oral Oncol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1093\/annonc\/mdz138","article-title":"Timing of HPV16-E6 Antibody Seroconversion before OPSCC: Findings from the HPVC3 Consortium","volume":"30","author":"Kreimer","year":"2019","journal-title":"Ann. Oncol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2748","DOI":"10.1002\/ijc.30697","article-title":"Sensitivity and Specificity of Antibodies against HPV16 E6 and Other Early Proteins for the Detection of HPV16-Driven Oropharyngeal Squamous Cell Carcinoma","volume":"140","author":"Holzinger","year":"2017","journal-title":"Int. J. Cancer"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"4382","DOI":"10.1002\/cncr.30966","article-title":"Human Papillomavirus 16 E6 Antibodies Are Sensitive for Human Papillomavirus-Driven Oropharyngeal Cancer and Are Associated with Recurrence","volume":"123","author":"Kreimer","year":"2017","journal-title":"Cancer"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Eberly, H.W., Sciscent, B.Y., Lorenz, F.J., Rettig, E.M., and Goyal, N. (2024). Current and Emerging Diagnostic, Prognostic, and Predictive Biomarkers in Head and Neck Cancer. Biomedicines, 12.","DOI":"10.3390\/biomedicines12020415"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1847","DOI":"10.1002\/ijc.34439","article-title":"Tumorigenic Activation around HPV Integrated Sites in Head and Neck Squamous Cell Carcinoma","volume":"152","author":"Mima","year":"2023","journal-title":"Int. J. Cancer"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"4550","DOI":"10.1200\/JCO.2013.50.3870","article-title":"Worldwide Trends in Incidence Rates for Oral Cavity and Oropharyngeal Cancers","volume":"31","author":"Chaturvedi","year":"2013","journal-title":"J. Clin. Oncol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S1470-2045(23)00013-X","article-title":"Prognostic Implications of P16 and HPV Discordance in Oropharyngeal Cancer (HNCIG-EPIC-OPC): A Multicentre, Multinational, Individual Patient Data Analysis","volume":"24","author":"Mehanna","year":"2023","journal-title":"Lancet Oncol."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Gameiro, S.F., Salnikov, M.Y., Zeng, P.Y.F., Barrett, J.W., Nichols, A.C., and Mymryk, J.S. (2023). HPV16 Intratypic Variants in Head and Neck Cancers: A North American Perspective. Viruses, 15.","DOI":"10.3390\/v15122411"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Huang, X., Duijf, P.H.G., Sriram, S., Perera, G., Vasani, S., Kenny, L., Leo, P., and Punyadeera, C. (2023). Circulating tumour DNA alterations: Emerging biomarker in head and neck squamous cell carcinoma. J. Biomed. Sci., 30.","DOI":"10.1186\/s12929-023-00953-z"},{"key":"ref_57","first-page":"775","article-title":"Cyclin D1 Oncoprotein Aberrantly Accumulates in Malignancies of Diverse Histogenesis","volume":"10","author":"Bartkova","year":"1995","journal-title":"Oncogene"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"105421","DOI":"10.1016\/j.oraloncology.2021.105421","article-title":"A Combination of Intra-Tumor Genetic Heterogeneity, Estrogen Receptor Alpha and Human Papillomavirus Status Predicts Outcomes in Head and Neck Squamous Cell Carcinoma Following Chemoradiotherapy","volume":"120","author":"Patel","year":"2021","journal-title":"Oral Oncol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"3707","DOI":"10.21873\/anticanres.15162","article-title":"Is There a Role for Sex Hormone Receptors in Head-and-Neck Cancer? Links with HPV Infection and Prognosis","volume":"41","author":"Brito","year":"2021","journal-title":"Anticancer Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1093\/jnci\/djy224","article-title":"Association of Estrogen Receptor Alpha Expression with Survival in Oropharyngeal Cancer Following Chemoradiation Therapy","volume":"111","author":"Koenigs","year":"2019","journal-title":"J. Natl. Cancer Inst."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1158\/1078-0432.CCR-21-3151","article-title":"Cell-Free HPV DNA Provides an Accurate and Rapid Diagnosis of HPV-Associated Head and Neck Cancer","volume":"28","author":"Siravegna","year":"2022","journal-title":"Clin. Cancer Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"3278","DOI":"10.1038\/s41598-024-53894-4","article-title":"Association of circulating tumor HPV16DNA levels and quantitative PET parameters in patients with HPV-positive head and neck squamous cell carcinoma","volume":"14","author":"Tatsumi","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1001\/jamaoto.2023.1937","article-title":"Performance of Liquid Biopsy for Diagnosis and Surveillance of Human Papillomavirus\u2013Associated Oropharyngeal Cancer","volume":"149","author":"Ferrandino","year":"2023","journal-title":"JAMA Otolaryngol. Head Neck Surg."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.1001\/jamaoto.2022.3282","article-title":"Association of Pretreatment Circulating Tumor Tissue-Modified Viral HPV DNA With Clinicopathologic Factors in HPV-Positive Oropharyngeal Cancer","volume":"148","author":"Rettig","year":"2022","journal-title":"JAMA Otolaryngol. Head Neck Surg."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1001\/jamaoto.2024.0350","article-title":"Preoperative Circulating Tumor HPV DNA and Oropharyngeal Squamous Cell Disease","volume":"150","author":"Lam","year":"2024","journal-title":"JAMA Otolaryngol. Head Neck Surg."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2193","DOI":"10.1002\/cncr.34109","article-title":"Cell-Free Human Papillomavirus DNA Kinetics after Surgery for Human Papillomavirus-Associated Oropharyngeal Cancer","volume":"128","author":"Siravegna","year":"2022","journal-title":"Cancer"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1016\/j.ijrobp.2022.02.012","article-title":"Detectable Postoperative Circulating Tumor Human Papillomavirus DNA and Association with Recurrence in Patients With HPV-Associated Oropharyngeal Squamous Cell Carcinoma","volume":"113","author":"Routman","year":"2022","journal-title":"Int. J. Radiat. Oncol. Biol. Phys."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1002\/ijc.33996","article-title":"Detection of Circulating Tumor Human Papillomavirus DNA before Diagnosis of HPV-Positive Head and Neck Cancer","volume":"151","author":"Rettig","year":"2022","journal-title":"Int. J. Cancer."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1001\/jamaoncol.2015.2524","article-title":"Prognostic Implication of Persistent Human Papillomavirus Type 16 DNA Detection in Oral Rinses for Human Papillomavirus-Related Oropharyngeal Carcinoma","volume":"1","author":"Rettig","year":"2015","journal-title":"JAMA Oncol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1001\/jamaoto.2014.1338","article-title":"Saliva and Plasma Quantitative Polymerase Chain Reaction-Based Detection and Surveillance of Human Papillomavirus-Related Head and Neck Cancer","volume":"140","author":"Ahn","year":"2014","journal-title":"JAMA Otolaryngol. Head Neck Surg."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"104958","DOI":"10.1016\/j.oraloncology.2020.104958","article-title":"cfDNA detection for HPV+ squamous cell carcinomas","volume":"115","author":"Roche","year":"2021","journal-title":"Oral Oncol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1038\/onc.2015.192","article-title":"The biology of circulating tumor cells","volume":"35","author":"Pantel","year":"2016","journal-title":"Oncogene"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.trsl.2018.07.003","article-title":"Circulating tumor cells as \u201cliquid biopsies\u201d to understand cancer metastasis","volume":"201","author":"Woo","year":"2018","journal-title":"Transl. Res."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.jconrel.2016.07.054","article-title":"Therapeutic and diagnostic applications of extracellular vesicles","volume":"244","author":"Stremersch","year":"2016","journal-title":"J. Control Release"},{"key":"ref_75","first-page":"241","article-title":"Les acides nucl\u00e9iques du plasma sanguin chez l\u2019Homme","volume":"142","author":"Mandel","year":"1948","journal-title":"C. R. Seances Soc. Biol. Fil."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"6083","DOI":"10.1038\/s41598-020-63180-8","article-title":"A comparative study of extracellular vesicle-associated and cell-free DNA and RNA for HPV detection in oropharyngeal squamous cell carcinoma","volume":"10","author":"Nguyen","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"3104","DOI":"10.1158\/1078-0432.CCR-19-3015","article-title":"Harmonizing Cell-Free DNA Collection and Processing Practices through Evidence-Based Guidance","volume":"26","author":"Greytak","year":"2020","journal-title":"Clin. Cancer Res."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1186\/s12967-020-02330-y","article-title":"Circulating HPV16 DNA may complement imaging assessment of early treatment efficacy in patients with HPV-positive oropharyngeal cancer","volume":"18","author":"Rutkowski","year":"2020","journal-title":"J. Transl. Med."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1002\/hed.25425","article-title":"Posttreatment surveillance PET\/CT for HPV-associated oropharyngeal cancer","volume":"41","author":"Corpman","year":"2019","journal-title":"Head Neck"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.oraloncology.2018.01.023","article-title":"Pitfalls of post-treatment PET after de-intensified chemoradiotherapy for HPV-associated oropharynx cancer: Secondary analysis of a phase 2 trial","volume":"78","author":"Wang","year":"2018","journal-title":"Oral Oncol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1200\/JCO.19.02444","article-title":"Plasma Circulating Tumor HPV DNA for the Surveillance of Cancer Recurrence in HPV-Associated Oropharyngeal Cancer","volume":"38","author":"Chera","year":"2020","journal-title":"J. Clin. Oncol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1038\/bjc.2017.258","article-title":"Predicting response to radical (chemo)radiotherapy with circulating HPV DNA in locally advanced head and neck squamous carcinoma","volume":"117","author":"Lee","year":"2017","journal-title":"Br. J. Cancer"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1146\/annurev.immunol.26.021607.090331","article-title":"PD-1 and Its Ligands in Tolerance and Immunity","volume":"26","author":"Keir","year":"2008","journal-title":"Annu. Rev. Immunol."},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Qiao, X., Jiang, J., Pang, X., Huang, M., Tang, Y., Liang, X., and Tang, Y. (2020). The Evolving Landscape of PD-1\/PD-L1 Pathway in Head and Neck Cancer. Front. Immunol., 11.","DOI":"10.3389\/fimmu.2020.01721"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.oraloncology.2019.03.012","article-title":"Significant association of PD-L1 expression with human papillomavirus positivity and its prognostic impact in oropharyngeal cancer","volume":"92","author":"Hong","year":"2019","journal-title":"Oral Oncol."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Cao, S., Wylie, K.M., Wyczalkowski, M.A., Karpova, A., Ley, J., Sun, S., Mashl, R.J., Liang, W.W., Wang, X., and Johnson, K. (2019). Dynamic host immune response in virus-associated cancers. Commun. Biol., 2.","DOI":"10.1038\/s42003-019-0352-3"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1016\/S1470-2045(16)30066-3","article-title":"Safety and Clinical Activity of Pembrolizumab for Treatment of Recurrent or Metastatic Squamous Cell Carcinoma of the Head and Neck (KEYNOTE-012): An Open-Label, Multicentre, Phase 1b Trial","volume":"17","author":"Seiwert","year":"2016","journal-title":"Lancet Oncol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1016\/S1368-8375(03)00048-4","article-title":"Prognostic Significance of Cyclin D1 Amplification and Overexpression in Oral Squamous Cell Carcinomas","volume":"39","author":"Miyamoto","year":"2003","journal-title":"Oral Oncol."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s12032-018-1241-1","article-title":"Characteristics and Impact of Programmed Death-Ligand 1 Expression, CD8+ Tumor-Infiltrating Lymphocytes, and P16 Status in Head and Neck Squamous Cell Carcinoma","volume":"36","author":"Ngamphaiboon","year":"2019","journal-title":"Med. Oncol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/j.pathol.2017.04.004","article-title":"PD-L1 Expression Predicts Longer Disease Free Survival in High Risk Head and Neck Cutaneous Squamous Cell Carcinoma","volume":"49","author":"Roper","year":"2017","journal-title":"Pathology"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"52889","DOI":"10.18632\/oncotarget.17547","article-title":"PD-L1: A Novel Prognostic Biomarker in Head and Neck Squamous Cell Carcinoma","volume":"8","author":"Braun","year":"2017","journal-title":"Oncotarget"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1038\/s41571-022-00603-7","article-title":"HPV-associated oropharyngeal cancer: Epidemiology, molecular biology and clinical management","volume":"19","author":"Lechner","year":"2022","journal-title":"Nat. Rev. Clin. Oncol."},{"key":"ref_93","doi-asserted-by":"crossref","unstructured":"Liouta, G., Adamaki, M., Tsintarakis, A., Zoumpourlis, P., Liouta, A., Agelaki, S., and Zoumpourlis, V. (2023). DNA Methylation as a Diagnostic, Prognostic, and Predictive Biomarker in Head and Neck Cancer. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24032996"},{"key":"ref_94","doi-asserted-by":"crossref","unstructured":"Wang, T.-H., Hsia, S.-M., Shih, Y.-H., and Shieh, T.-M. (2017). Association of Smoking, Alcohol Use, and Betel Quid Chewing with Epigenetic Aberrations in Cancers. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18061210"},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"Camuzi, D., de Almeida Sim\u00e3o, T., Dias, F., Ribeiro Pinto, L.F., and Soares-Lima, S.C. (2021). Head and Neck Cancers Are Not Alike When Tarred with the Same Brush: An Epigenetic Perspective from the Cancerization Field to Prognosis. Cancers, 13.","DOI":"10.3390\/cancers13225630"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"e003420","DOI":"10.1136\/jitc-2021-003420","article-title":"DNA Methylation Profiles Differ in Responders versus Non-Responders to Anti-PD-1 Immune Checkpoint Inhibitors in Patients with Advanced and Metastatic Head and Neck Squamous Cell Carcinoma","volume":"10","author":"Starzer","year":"2022","journal-title":"J. Immunother. Cancer"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"93","DOI":"10.3322\/caac.21388","article-title":"The eighth edition AJCC cancer staging manual: Continuing to build a bridge from a population-based to a more \u201cpersonalized\u201d approach to cancer staging","volume":"67","author":"Amin","year":"2017","journal-title":"CA Cancer J. Clin."},{"key":"ref_98","unstructured":"(2024, June 08). Available online: https:\/\/ecancer.org\/en\/news\/23659-researchers-develop-novel-dna-biosensor-for-early-diagnosis-of-cervical-cancer."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"300","DOI":"10.4143\/crt.2018.012","article-title":"Investigating the feasibility of targeted next-generation sequencing to guide the treatment of head and neck squamous cell carcinoma","volume":"51","author":"Lim","year":"2019","journal-title":"Cancer Res Treat."},{"key":"ref_100","unstructured":"Radiation Therapy with or without Cisplatin in Treating Patients with Stage III-IVA Squamous Cell Carcinoma of the Head and Neck Who Have Undergone Surgery (2024, June 08). ClinicalTrials.gov, Available online: https:\/\/www.clinicaltrials.gov\/ct2\/show\/NCT02734537."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1001\/jamaoto.2019.2427","article-title":"Prognostic value of tumor-infiltrating lymphocytes in head and neck squamous cell carcinoma","volume":"145","author":"Spector","year":"2019","journal-title":"JAMA Otolaryngol. Head Neck Surg."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1177\/01945998211013765","article-title":"Tumor-infiltrating lymphocytes in patients with advanced laryngeal cancer undergoing bioselection","volume":"166","author":"Smith","year":"2022","journal-title":"Otolaryngol. Head Neck Surg."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1038\/s41416-019-0409-6","article-title":"Tissue-infiltrating immune cells as prognostic markers in oral squamous cell carcinoma: A systematic review and meta-analysis","volume":"120","author":"Wirsing","year":"2019","journal-title":"Br. J. Cancer"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"7809","DOI":"10.1021\/am509096x","article-title":"Comparative study of potential applications of graphene, MoS2, and other two-dimensional materials in energy devices, sensors, and related areas","volume":"7","author":"Rao","year":"2015","journal-title":"ACS Appl. Mater. Inter."},{"key":"ref_105","doi-asserted-by":"crossref","unstructured":"Kim, Y., and Kang, E. (2023). A graphitic nano-onion\/molybdenum disulfide nanosheet composite as a platform for HPV-associated cancer-detecting DNA biosensors. J. Nanobiotechnol., 21.","DOI":"10.1186\/s12951-023-01948-6"},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.bios.2017.06.011","article-title":"Recent advances in transition-metal dichalcogenides based electrochemical biosensors: A review","volume":"97","author":"Wang","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.bios.2018.02.035","article-title":"A comprehensive review on nano-molybdenum disulfide\/DNA interfaces as emerging biosensing platforms","volume":"107","author":"Kukkar","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"45814","DOI":"10.1021\/acsami.0c13385","article-title":"Electrochemical DNA sensors based on MoS2-AuNPs for polynucleotide kinase activity and inhibition assay","volume":"12","author":"Lin","year":"2020","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"145839","DOI":"10.1016\/j.apsusc.2020.145839","article-title":"UV-induced modification of PEDOT:PSS-based nanocomposite films investigated by Raman microscopy mapping","volume":"513","author":"Toto","year":"2020","journal-title":"Appl. Surf. Sci."},{"key":"ref_110","doi-asserted-by":"crossref","unstructured":"Kumar, M., Wang, M., Kumara Swamy, B.E., Praveen, M., and Zhao, W. (2020). Poly (alanine)\/NaOH\/MoS2\/MWCNTs modified carbon paste electrode for simultaneous detection of dopamine, ascorbic acid, serotonin and guanine. Colloids Surf. B Biointerfaces, 196.","DOI":"10.1016\/j.colsurfb.2020.111299"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1021\/acsaelm.9b00632","article-title":"Transport properties of a molybdenum disulfide and carbon dot nanohybrid transistor and its applications as a Hg2+ aptasensor","volume":"2","author":"Majd","year":"2020","journal-title":"ACS Appl. Electron. Mater."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"136183","DOI":"10.1016\/j.cej.2022.136183","article-title":"Applications of carbon-based conductive nanomaterials in biosensors","volume":"442","author":"Noruzi","year":"2022","journal-title":"Chem. Eng. J."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.bios.2017.06.001","article-title":"Carbon nanostructures as immobilization platform for DNA: A review on current progress in electrochemical DNA sensors","volume":"97","author":"Rasheed","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"5449","DOI":"10.1021\/acsanm.0c00782","article-title":"Conducting polymer-reduced graphene oxide sponge electrode for electrochemical detection based on dna hybridization","volume":"3","author":"Kiransan","year":"2020","journal-title":"ACS Appl. Nano Mater."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1016\/j.snb.2018.12.164","article-title":"A new epirubicin biosensor based on amplifying DNA interactions with polypyrrole and nitrogen-doped reduced graphene: Experimental and docking theoretical investigations","volume":"284","author":"Khodadadi","year":"2019","journal-title":"Sens. Actuators B-Chem."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.1021\/acs.langmuir.1c02891","article-title":"Construction of a molybdenum disulfide-based colorimetric sensor for label-free infectious disease analysis coupled with a catalyzed hairpin assembly reaction","volume":"38","author":"Dong","year":"2022","journal-title":"Langmuir"},{"key":"ref_117","doi-asserted-by":"crossref","unstructured":"Ji, R., Niu, W., Chen, S., Xu, W., Ji, X., Yuan, L., Zhao, H., Geng, M., Qiu, J., and Li, C. (2019). Target-inspired Pb(2+)-dependent DNAzyme for ultrasensitive electrochemical sensor based on MoS2-AuPt nanocomposites and hemin\/G-quadruplex DNAzyme as signal amplifier. Biosens. Bioelectron., 144.","DOI":"10.1016\/j.bios.2019.111560"},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1016\/j.snb.2018.02.065","article-title":"Nucleic aptamer modified porous reduced graphene oxide\/MoS2 based electrodes for viral detection: Application to human papillomavirus (HPV)","volume":"262","author":"Chekin","year":"2018","journal-title":"Sens. Actuat B-Chem."},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.aca.2013.12.005","article-title":"Characterization of an electrochemical mercury sensor using alternating current, cyclic, square wave and differential pulse voltammetry","volume":"810","author":"Guerreiro","year":"2014","journal-title":"Anal. Chim. Acta"},{"key":"ref_120","doi-asserted-by":"crossref","unstructured":"Wei, Y., Zhao, Z., and Ma, X. (2022). Description of CRISPR-Cas9 development and its prospects in human papillomavirus-driven cancer treatment. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.1037124"},{"key":"ref_121","doi-asserted-by":"crossref","unstructured":"Lin, H., Li, G., Peng, X., Deng, A., Ye, L., Shi, L., Wang, T., and He, J. (2021). The Use of CRISPR\/Cas9 as a Tool to Study Human Infectious Viruses. Front. Cell Infect. Microbiol., 11.","DOI":"10.3389\/fcimb.2021.590989"},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1038\/s41417-021-00366-w","article-title":"The application of CRISPR\/Cas9 system in cervical carcinogenesis","volume":"29","author":"Gao","year":"2022","journal-title":"Cancer Gene Ther."},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1038\/s41551-021-00760-7","article-title":"CRISPR-based diagnostics","volume":"5","author":"Kaminski","year":"2021","journal-title":"Nat. Biomed. Eng."},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1021\/acssynbio.2c00496","article-title":"CRISPR-Based Diagnostics: Challenges and Potential Solutions toward Point-of-Care Applications","volume":"12","author":"Ghouneimy","year":"2023","journal-title":"ACS Synth. Biol."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"11301","DOI":"10.1007\/s11033-022-07752-z","article-title":"The CRISPR-Cas system as a tool for diagnosing and treating infectious diseases","volume":"49","author":"Lou","year":"2022","journal-title":"Mol. Biol. Rep."},{"key":"ref_126","doi-asserted-by":"crossref","unstructured":"Puig-Serra, P., Casado-Rosas, M.C., Martinez-Lage, M., Olalla-Sastre, B., Alonso-Yanez, A., Torres-Ruiz, R., and Rodriguez-Perales, S. (2022). CRISPR Approaches for the Diagnosis of Human Diseases. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23031757"},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/s12943-023-01925-5","article-title":"Comprehensive review of CRISPR-based gene editing: Mechanisms, challenges, and applications in cancer therapy","volume":"23","author":"Chehelgerdi","year":"2024","journal-title":"Mol. Cancer"},{"key":"ref_128","doi-asserted-by":"crossref","unstructured":"Stefanoudakis, D., Kathuria-Prakash, N., Sun, A.W., Abel, M., Drolen, C.E., Ashbaugh, C., Zhang, S., Hui, G., Tabatabaei, Y.A., and Zektser, Y. (2023). The Potential Revolution of Cancer Treatment with CRISPR Technology. Cancers, 15.","DOI":"10.3390\/cancers15061813"},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.3390\/curroncol30020152","article-title":"Application of CRISPR\/Cas9 Technology in Cancer Treatment: A Future Direction","volume":"30","author":"Rabaan","year":"2023","journal-title":"Curr. Oncol."},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"2483","DOI":"10.1007\/s00432-022-04072-4","article-title":"The Prognostic Role of PSMD14 in Head and Neck Squamous Cell Carcinoma","volume":"149","author":"Schnoell","year":"2023","journal-title":"J. Cancer Res. Clin. Oncol."},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"3882","DOI":"10.1002\/cncr.26718","article-title":"Exploring Biomarkers in Head and Neck Cancer","volume":"118","author":"Langer","year":"2012","journal-title":"Cancer"},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1093\/annonc\/mdp002","article-title":"Class III Beta-Tubulin, but Not ERCC1, Is a Strong Predictive and Prognostic Marker in Locally Advanced Head and Neck Squamous Cell Carcinoma","volume":"20","author":"Koh","year":"2009","journal-title":"Ann. Oncol."},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"2655","DOI":"10.7150\/thno.48375","article-title":"Blockade of Deubiquitinating Enzyme PSMD14 Overcomes Chemoresistance in Head and Neck Squamous Cell Carcinoma by Antagonizing E2F1\/Akt\/SOX2-Mediated Stemness","volume":"11","author":"Jing","year":"2021","journal-title":"Theranostics"},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1186\/s12967-023-04052-3","article-title":"Identification of the MMP Family as Therapeutic Targets and Prognostic Biomarkers in the Microenvironment of Head and Neck Squamous Cell Carcinoma","volume":"21","author":"Liu","year":"2023","journal-title":"J. Transl. Med."},{"key":"ref_135","doi-asserted-by":"crossref","unstructured":"Campo, F., Paolini, F., Manciocco, V., Moretto, S., Pichi, B., Moretti, C., Blandino, G., De Pascale, V., Benevolo, M., and Pimpinelli, F. Circulating tumor HPV DNA in the management of HPV+ oropharyngeal cancer and its correlation with MRI. Head Neck, 2024.","DOI":"10.1002\/hed.27866"},{"key":"ref_136","doi-asserted-by":"crossref","unstructured":"Kais, A., Santiago, S.P., Han, P.C., Clump, D.A., Stokes, W.A., Fancy, T., Cui, R., Martin, E., and Turner, M.T. (2024). Human papillomavirus circulating tumor DNA: A diagnostic tool in squamous cell carcinoma of unknown primary-a pilot study. Front. Oncol., 14.","DOI":"10.3389\/fonc.2024.1376595"},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1016\/j.jasc.2021.06.008","article-title":"p16 immunostaining in fine-needle aspirations of the head and neck: Determining the optimal positivity threshold in HPV-related squamous cell cancer","volume":"10","author":"Wang","year":"2021","journal-title":"J. Am. Soc. Cytopathol."},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1158\/1940-6207.CAPR-19-0185","article-title":"Evaluating the Utility and Prevalence of HPV Biomarkers in Oral Rinses and Serology for HPV-related Oropharyngeal Cancer","volume":"12","author":"Clemens","year":"2019","journal-title":"Cancer Prev. Res."},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1097\/PAS.0000000000001685","article-title":"Oropharyngeal Squamous Cell Carcinoma with Discordant p16 and HPV mRNA Results: Incidence and Characterization in a Large, Contemporary United States Cohort","volume":"45","author":"Shinn","year":"2021","journal-title":"Am. J. Surg. Pathol."},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"105805","DOI":"10.1016\/j.oraloncology.2022.105805","article-title":"Comparison of next generation sequencing, droplet digital PCR, and quantitative real-time PCR for the earlier detection and quantification of HPV in HPV-positive oropharyngeal cancer","volume":"128","author":"Mattox","year":"2022","journal-title":"Oral Oncol."},{"key":"ref_141","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.jmoldx.2023.11.007","article-title":"Comparing the Diagnostic Performance of Quantitative PCR, Digital Droplet PCR, and Next-Generation Sequencing Liquid Biopsies for Human Papillomavirus-Associated Cancers","volume":"26","author":"Naegele","year":"2024","journal-title":"J. Mol. Diagn. JMD"}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/14\/8\/925\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:26:41Z","timestamp":1760110001000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/14\/8\/925"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,30]]},"references-count":141,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["biom14080925"],"URL":"https:\/\/doi.org\/10.3390\/biom14080925","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,30]]}}}