{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:59:14Z","timestamp":1792803554209,"version":"4.5.0"},"reference-count":45,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T00:00:00Z","timestamp":1724371200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004836","name":"Danish Council for Independent Research","doi-asserted-by":"publisher","award":["6111-00298B"],"award-info":[{"award-number":["6111-00298B"]}],"id":[{"id":"10.13039\/501100004836","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004836","name":"Danish Council for Independent Research","doi-asserted-by":"publisher","award":["101002060"],"award-info":[{"award-number":["101002060"]}],"id":[{"id":"10.13039\/501100004836","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004836","name":"Danish Council for Independent Research","doi-asserted-by":"publisher","award":["R163-2013-15402"],"award-info":[{"award-number":["R163-2013-15402"]}],"id":[{"id":"10.13039\/501100004836","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Research Council under the European Union\u2019s Horizon 2020","award":["6111-00298B"],"award-info":[{"award-number":["6111-00298B"]}],"id":[{"id":"https:\/\/ror.org\/0472cxd90","id-type":"ROR","asserted-by":"crossref"}]},{"name":"European Research Council under the European Union\u2019s Horizon 2020","award":["101002060"],"award-info":[{"award-number":["101002060"]}],"id":[{"id":"https:\/\/ror.org\/0472cxd90","id-type":"ROR","asserted-by":"crossref"}]},{"name":"European Research Council under the European Union\u2019s Horizon 2020","award":["R163-2013-15402"],"award-info":[{"award-number":["R163-2013-15402"]}],"id":[{"id":"https:\/\/ror.org\/0472cxd90","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Lundbeck Foundatio","award":["6111-00298B"],"award-info":[{"award-number":["6111-00298B"]}],"id":[{"id":"https:\/\/ror.org\/03hz8wd80","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Lundbeck Foundatio","award":["101002060"],"award-info":[{"award-number":["101002060"]}],"id":[{"id":"https:\/\/ror.org\/03hz8wd80","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Lundbeck Foundatio","award":["R163-2013-15402"],"award-info":[{"award-number":["R163-2013-15402"]}],"id":[{"id":"https:\/\/ror.org\/03hz8wd80","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>The growing demand for effective alternatives to red blood cells (RBCs) has spurred significant research into hemoglobin (Hb)-based oxygen carriers (HBOCs). Accurate characterization of HBOCs\u2014including Hb content, encapsulation efficiency, and yield\u2014is crucial for ensuring effective oxygen delivery, economic viability, and the prevention of adverse effects caused by free Hb. However, the choice of quantification methods for HBOCs is often driven more by tradition than by a thorough assessment of available options. This study meticulously compares various UV-vis spectroscopy-based methods for Hb quantification, focusing on their efficacy in measuring Hb extracted from bovine RBCs across different concentration levels. The findings identify the sodium lauryl sulfate Hb method as the preferred choice due to its specificity, ease of use, cost-effectiveness, and safety, particularly when compared to cyanmethemoglobin-based methods. Additionally, the study discusses the suitability of these methods for HBOC characterization, emphasizing the importance of considering carrier components and potential interferences by analyzing the absorbance spectrum before selecting a method. Overall, this study provides valuable insights into the selection of accurate and reliable Hb quantification methods, which are essential for rigorous HBOC characterization and advancements in medical research.<\/jats:p>","DOI":"10.3390\/biom14091046","type":"journal-article","created":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T12:53:19Z","timestamp":1724417599000},"page":"1046","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Comparative Evaluation of UV-Vis Spectroscopy-Based Approaches for Hemoglobin Quantification: Method Selection and Practical Insights"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9810-4753","authenticated-orcid":false,"given":"Clara","family":"Coll-Satue","sequence":"first","affiliation":[{"name":"Center for Nanomedicine and Theranostics, Department of Health Technology, Technical University of Denmark, Nils Koppels All\u00e9, Building 423, 2800 Kongens Lyngby, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9269-4825","authenticated-orcid":false,"given":"Michelle Maria Theresia","family":"Jansman","sequence":"additional","affiliation":[{"name":"Center for Nanomedicine and Theranostics, Department of Health Technology, Technical University of Denmark, Nils Koppels All\u00e9, Building 423, 2800 Kongens Lyngby, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8177-4806","authenticated-orcid":false,"given":"Leticia","family":"Hosta-Rigau","sequence":"additional","affiliation":[{"name":"Center for Nanomedicine and Theranostics, Department of Health Technology, Technical University of Denmark, Nils Koppels All\u00e9, Building 423, 2800 Kongens Lyngby, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1039\/D0BM01767A","article-title":"Stepping Stones to the Future of Haemoglobin-Based Blood Products: Clinical, Preclinical and Innovative Examples","volume":"9","author":"Bishnoi","year":"2021","journal-title":"Biomater. 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