{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T22:35:39Z","timestamp":1783463739539,"version":"3.55.0"},"reference-count":45,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,8,25]],"date-time":"2024-08-25T00:00:00Z","timestamp":1724544000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["82370336"],"award-info":[{"award-number":["82370336"]}]},{"name":"National Natural Science Foundation of China","award":["81900302"],"award-info":[{"award-number":["81900302"]}]},{"name":"National Natural Science Foundation of China","award":["81300133"],"award-info":[{"award-number":["81300133"]}]},{"name":"National Natural Science Foundation of China","award":["2018M641869"],"award-info":[{"award-number":["2018M641869"]}]},{"name":"National Natural Science Foundation of China","award":["2022IIT206"],"award-info":[{"award-number":["2022IIT206"]}]},{"name":"National Natural Science Foundation of China","award":["HYD2020YQ0001"],"award-info":[{"award-number":["HYD2020YQ0001"]}]},{"name":"China Postdoctoral Science Foundation","award":["82370336"],"award-info":[{"award-number":["82370336"]}]},{"name":"China Postdoctoral Science Foundation","award":["81900302"],"award-info":[{"award-number":["81900302"]}]},{"name":"China Postdoctoral Science Foundation","award":["81300133"],"award-info":[{"award-number":["81300133"]}]},{"name":"China Postdoctoral Science Foundation","award":["2018M641869"],"award-info":[{"award-number":["2018M641869"]}]},{"name":"China Postdoctoral Science Foundation","award":["2022IIT206"],"award-info":[{"award-number":["2022IIT206"]}]},{"name":"China Postdoctoral Science Foundation","award":["HYD2020YQ0001"],"award-info":[{"award-number":["HYD2020YQ0001"]}]},{"name":"First Affiliated Hospital of Harbin Medical University","award":["82370336"],"award-info":[{"award-number":["82370336"]}]},{"name":"First Affiliated Hospital of Harbin Medical University","award":["81900302"],"award-info":[{"award-number":["81900302"]}]},{"name":"First Affiliated Hospital of Harbin Medical University","award":["81300133"],"award-info":[{"award-number":["81300133"]}]},{"name":"First Affiliated Hospital of Harbin Medical University","award":["2018M641869"],"award-info":[{"award-number":["2018M641869"]}]},{"name":"First Affiliated Hospital of Harbin Medical University","award":["2022IIT206"],"award-info":[{"award-number":["2022IIT206"]}]},{"name":"First Affiliated Hospital of Harbin Medical University","award":["HYD2020YQ0001"],"award-info":[{"award-number":["HYD2020YQ0001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Atrial fibrillation (AF) is the most common sustained arrhythmia to affect 1% of the global population and increases with age. Atrial fibrosis is a crucial substrate for promoting structural remodeling to cause atrial arrhythmogenesis. Bone morphogenic protein 2 (BMP2) has been reported to be involved in cardiac fibrogenesis. However, its role in modulating atrial fibrosis to affect AF development remains unknown. Our study aimed to investigate the expression of BMP2 under different AF conditions and the effect of BMP2 on the progression of atrial fibrosis using an angiotensin II (Ang II) rat model and an ex vivo cardiac fibroblast model. The qRT-PCR and Western blot assay showed increased BMP2 mRNA and protein levels in the atria of chronic AF patients and the right atria of a tachypacing rabbit model. In contrast, the levels of BMP2 receptor mRNA were comparable. The AF incidence of the Ang II rat was higher than that of a control rat, which was reduced by BMP2 treatment. Masson staining demonstrated an anti-fibrogenic impact on BMP2-subjected rat atria compared to only Ang II-treated rat atria. RNA-sequencing indicated the potential function of blocking NLRP3-associted inflammasome activation in BMP2-treated rat atrial tissues. In vitro, transfecting BMP2 shRNA into neonatal rat atrial fibroblasts upregulated the mRNA levels of NLRP3\/Caspase-1\/p20\/ASC and the secretion of IL-1\u03b2 and IL-6. In contrast, recombinant BMP2 protein attenuated the increased levels of the NLRP3 inflammasome pathway induced by Ang II. In summary, BMP2 opposes atrial fibrosis to alleviate AF susceptibility by inhibiting the activation of the inflammasome in atrial fibroblasts.<\/jats:p>","DOI":"10.3390\/biom14091053","type":"journal-article","created":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T01:48:49Z","timestamp":1724636929000},"page":"1053","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["BMP2 Diminishes Angiotensin II-Induced Atrial Fibrillation by Inhibiting NLRP3 Inflammasome Signaling in Atrial Fibroblasts"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3980-0630","authenticated-orcid":false,"given":"Yue","family":"Yuan","sequence":"first","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erwen","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of General Medicine, The First Affiliated Hospital, Harbin Medical University, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinbo","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyuan","family":"Mu","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingzuo","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanye","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuling","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Sheng","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1136\/heartjnl-2020-318480","article-title":"Atrial fibrillation: Global burdens and global opportunities","volume":"107","author":"Kornej","year":"2021","journal-title":"Heart"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1093\/ehjqcco\/qcaa061","article-title":"Global, regional, and national prevalence, incidence, mortality, and risk factors for atrial fibrillation, 1990\u20132017: Results from the Global Burden of Disease Study 2017","volume":"7","author":"Dai","year":"2021","journal-title":"Eur. 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