{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T10:17:03Z","timestamp":1773397023862,"version":"3.50.1"},"reference-count":22,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T00:00:00Z","timestamp":1725235200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Regional Development Fund and the Republic of Cyprus","award":["EXCELLENCE\/0421\/0152"],"award-info":[{"award-number":["EXCELLENCE\/0421\/0152"]}]},{"name":"European Regional Development Fund and the Republic of Cyprus","award":["CCRI_2020_FUN_001-103"],"award-info":[{"award-number":["CCRI_2020_FUN_001-103"]}]},{"name":"European Regional Development Fund and the Republic of Cyprus","award":["RP220330"],"award-info":[{"award-number":["RP220330"]}]},{"name":"Cyprus Cancer Research Institute\u2019s (C.C.R.I) Bridges in research excellence grant","award":["EXCELLENCE\/0421\/0152"],"award-info":[{"award-number":["EXCELLENCE\/0421\/0152"]}]},{"name":"Cyprus Cancer Research Institute\u2019s (C.C.R.I) Bridges in research excellence grant","award":["CCRI_2020_FUN_001-103"],"award-info":[{"award-number":["CCRI_2020_FUN_001-103"]}]},{"name":"Cyprus Cancer Research Institute\u2019s (C.C.R.I) Bridges in research excellence grant","award":["RP220330"],"award-info":[{"award-number":["RP220330"]}]},{"DOI":"10.13039\/100004917","name":"Cancer Prevention and Research Institute of Texas","doi-asserted-by":"publisher","award":["EXCELLENCE\/0421\/0152"],"award-info":[{"award-number":["EXCELLENCE\/0421\/0152"]}],"id":[{"id":"10.13039\/100004917","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004917","name":"Cancer Prevention and Research Institute of Texas","doi-asserted-by":"publisher","award":["CCRI_2020_FUN_001-103"],"award-info":[{"award-number":["CCRI_2020_FUN_001-103"]}],"id":[{"id":"10.13039\/100004917","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004917","name":"Cancer Prevention and Research Institute of Texas","doi-asserted-by":"publisher","award":["RP220330"],"award-info":[{"award-number":["RP220330"]}],"id":[{"id":"10.13039\/100004917","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>N-alpha-acetyltransferase 40 (NAA40) is an evolutionarily conserved N-terminal acetyltransferase (NAT) linked to oncogenesis and chemoresistance. A recent study reported the generation of a second, shorter NAA40 isoform (NAA40S) through alternative translation, which we proceeded to further characterise. Notably, recombinant NAA40S had a greater in vitro enzymatic activity and affinity towards its histone H2A\/H4 substrates compared to full-length NAA40 (NAA40L). Within cells, NAA40S was enzymatically active, based on its ability to suppress the H2A\/H4S1Ph antagonistic mark in CRISPR-generated NAA40 knockout cells. Finally, we show that in addition to alternative translation, the NAA40S isoform could be derived from a primate and testis-specific transcript, which may align with the \u201cout-of-testis\u201d origin of recently evolved genes and isoforms. To summarise, our data reveal an even greater functional divergence between the two NAA40 isoforms than had been previously recognised.<\/jats:p>","DOI":"10.3390\/biom14091100","type":"journal-article","created":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T06:51:17Z","timestamp":1725259877000},"page":"1100","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Biochemical Characterisation of the Short Isoform of Histone N-Terminal Acetyltransferase NAA40"],"prefix":"10.3390","volume":"14","author":[{"given":"Ariel","family":"Klavaris","sequence":"first","affiliation":[{"name":"Epigenetics and Gene Regulation Laboratory, Department of Biological Sciences, University of Cyprus, Nicosia 2109, Cyprus"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Kouma","sequence":"additional","affiliation":[{"name":"Epigenetics and Gene Regulation Laboratory, Department of Biological Sciences, University of Cyprus, Nicosia 2109, Cyprus"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9501-503X","authenticated-orcid":false,"given":"Cem","family":"Ozdemir","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1017-2677","authenticated-orcid":false,"given":"Vicky","family":"Nicolaidou","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Nicosia, Nicosia 2417, Cyprus"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4544-0013","authenticated-orcid":false,"given":"Kyle M.","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1222-5130","authenticated-orcid":false,"given":"Costas","family":"Koufaris","sequence":"additional","affiliation":[{"name":"Epigenetics and Gene Regulation Laboratory, Department of Biological Sciences, University of Cyprus, Nicosia 2109, Cyprus"},{"name":"Cyprus Cancer Research Institute, Nicosia 2109, Cyprus"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3748-8711","authenticated-orcid":false,"given":"Antonis","family":"Kirmizis","sequence":"additional","affiliation":[{"name":"Epigenetics and Gene Regulation Laboratory, Department of Biological Sciences, University of Cyprus, Nicosia 2109, Cyprus"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1016\/j.molcel.2019.02.007","article-title":"Co-translational, Post-translational, and Non-catalytic Roles of N-Terminal Acetyltransferases","volume":"73","author":"Aksnes","year":"2019","journal-title":"Mol. 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