{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:23:31Z","timestamp":1760145811244,"version":"build-2065373602"},"reference-count":70,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,9,3]],"date-time":"2024-09-03T00:00:00Z","timestamp":1725321600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Scientific and Technological Research Council of T\u00fcrkiye (T\u00dcB\u0130TAK)","award":["1059B192300559"],"award-info":[{"award-number":["1059B192300559"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Alzheimer\u2019s disease is a severe neurodegenerative disorder, and the discovery of biomarkers is crucial for early diagnosis. While the analysis of biomarkers in cerebrospinal fluid is well accepted, there are currently no blood biomarkers available. Our research focuses on identifying novel plasma biomarkers for Alzheimer\u2019s disease. To achieve this, we employed a technique that involves coupling human plasma to mouse organotypic brain slices via microcontact prints. After culturing for two weeks, we assessed Iba1-immunopositive microglia on these microcontact prints. We hypothesized that plasma from Alzheimer\u2019s patients contains factors that affect microglial migration. Our data indicated that plasma from Alzheimer\u2019s patients significantly inhibited the migration of round Iba1-immunoreactive microglia (13 \u00b1 3, n = 24, p = 0.01) compared to healthy controls (50 \u00b1 16, n = 23). Based on these findings, we selected the most promising plasma samples and conducted mass spectrometry using a differential approach, and we identified four potential biomarkers: mannose-binding protein C, macrophage receptor MARCO, complement factor H-related protein-3, and C-reactive protein. Our method represents a novel and innovative approach to translate research findings from mouse models to human applications.<\/jats:p>","DOI":"10.3390\/biom14091109","type":"journal-article","created":{"date-parts":[[2024,9,3]],"date-time":"2024-09-03T08:38:47Z","timestamp":1725352727000},"page":"1109","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["From Organotypic Mouse Brain Slices to Human Alzheimer Plasma Biomarkers: A Focus on Microglia"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-2449-4885","authenticated-orcid":false,"given":"Katharina","family":"Steiner","sequence":"first","affiliation":[{"name":"Laboratory of Psychiatry and Experimental Alzheimer\u2019s Research, Medical University of Innsbruck, 6020 Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1759-3052","authenticated-orcid":false,"given":"Sakir Necat","family":"Yilmaz","sequence":"additional","affiliation":[{"name":"Laboratory of Psychiatry and Experimental Alzheimer\u2019s Research, Medical University of Innsbruck, 6020 Innsbruck, Austria"},{"name":"Department of Histology and Embryology, Faculty of Medicine, Mersin University, Mersin 33110, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessa","family":"Gern","sequence":"additional","affiliation":[{"name":"Laboratory of Psychiatry and Experimental Alzheimer\u2019s Research, Medical University of Innsbruck, 6020 Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josef","family":"Marksteiner","sequence":"additional","affiliation":[{"name":"Department of Psychiatry and Psychotherapy A, Hall State Hospital, 6060 Hall in Tirol, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1646-3256","authenticated-orcid":false,"given":"Klaus","family":"Faserl","sequence":"additional","affiliation":[{"name":"Protein Core Facility, Institute of Medical Biochemistry, CCB-Biocenter, Medical University of Innsbruck, 6020 Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathias","family":"Villunger","sequence":"additional","affiliation":[{"name":"Protein Core Facility, Institute of Medical Biochemistry, CCB-Biocenter, Medical University of Innsbruck, 6020 Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bettina","family":"Sarg","sequence":"additional","affiliation":[{"name":"Protein Core Facility, Institute of Medical Biochemistry, CCB-Biocenter, Medical University of Innsbruck, 6020 Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7641-4240","authenticated-orcid":false,"given":"Christian","family":"Humpel","sequence":"additional","affiliation":[{"name":"Laboratory of Psychiatry and Experimental Alzheimer\u2019s Research, Medical University of Innsbruck, 6020 Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1373\/clinchem.2009.130518","article-title":"Amyloid-beta(1-42), total tau, and phosphorylated tau as cerebrospinal fluid biomarkers for the diagnosis of Alzheimer disease","volume":"56","author":"Mulder","year":"2010","journal-title":"Clin. 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