{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T07:12:23Z","timestamp":1782976343241,"version":"3.54.5"},"reference-count":44,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T00:00:00Z","timestamp":1727395200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MCIN\/AEI\/10.13039\/501100011033","award":["BFU2017-82805-C2-2-P"],"award-info":[{"award-number":["BFU2017-82805-C2-2-P"]}]},{"name":"MCIN\/AEI\/10.13039\/501100011033","award":["PID2020-112783GB-C22"],"award-info":[{"award-number":["PID2020-112783GB-C22"]}]},{"name":"MCIN\/AEI\/10.13039\/501100011033","award":["Proof of Concept-2020 H1-CBM"],"award-info":[{"award-number":["Proof of Concept-2020 H1-CBM"]}]},{"DOI":"10.13039\/501100011104","name":"Universitat Aut\u00f2noma de Barcelona","doi-asserted-by":"publisher","award":["BFU2017-82805-C2-2-P"],"award-info":[{"award-number":["BFU2017-82805-C2-2-P"]}],"id":[{"id":"10.13039\/501100011104","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011104","name":"Universitat Aut\u00f2noma de Barcelona","doi-asserted-by":"publisher","award":["PID2020-112783GB-C22"],"award-info":[{"award-number":["PID2020-112783GB-C22"]}],"id":[{"id":"10.13039\/501100011104","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011104","name":"Universitat Aut\u00f2noma de Barcelona","doi-asserted-by":"publisher","award":["Proof of Concept-2020 H1-CBM"],"award-info":[{"award-number":["Proof of Concept-2020 H1-CBM"]}],"id":[{"id":"10.13039\/501100011104","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Histone H1 is involved in the regulation of chromatin structure. Human somatic cells express up to seven subtypes. The variability in the proportions of somatic H1s (H1 complement) is one piece of evidence supporting their functional specificity. Alterations in the protein levels of different H1 subtypes have been observed in cancer, suggesting their potential as biomarkers and that they might play a role in disease development. We have developed a mass spectrometry-based (MS) parallel reaction monitoring (PRM) assay suitable for the quantification of H1 subtypes. Our PRM method is based on the quantification of unique peptides for each subtype, providing high specificity. Evaluation of the PRM performance on three human cell lines, HeLa, K562, and T47D, showed high reproducibility and sensitivity. Quantification values agreed with the electrophoretic and Western blot data, indicating the accuracy of the method. We used PRM to quantify the H1 complement in peripheral blood samples of healthy individuals and chronic myeloid leukemia (CML) patients. In CML, the first line of therapy is a tyrosine kinase inhibitor, imatinib. Our preliminary data revealed differences in the H1 complement in CML patients between imatinib responders and non-responders. These results support further research to determine if the H1 content or subtype composition could help predict imatinib response.<\/jats:p>","DOI":"10.3390\/biom14101221","type":"journal-article","created":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T06:10:27Z","timestamp":1727417427000},"page":"1221","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Quantification of Histone H1 Subtypes Using Targeted Proteomics"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8794-8440","authenticated-orcid":false,"given":"Jordi","family":"L\u00f3pez-G\u00f3mez","sequence":"first","affiliation":[{"name":"Biochemistry and Molecular Biology Department, Biosciences Faculty, Autonomous University of Barcelona, 08193 Bellaterra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4040-3280","authenticated-orcid":false,"given":"Laura","family":"Villarreal","sequence":"additional","affiliation":[{"name":"Institute for Research in Biomedicine (IRB Barcelona), 08028 Barcelona, Spain"},{"name":"The Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Andr\u00e9s","sequence":"additional","affiliation":[{"name":"Biochemistry and Molecular Biology Department, Biosciences Faculty, Autonomous University of Barcelona, 08193 Bellaterra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inma","family":"Ponte","sequence":"additional","affiliation":[{"name":"Biochemistry and Molecular Biology Department, Biosciences Faculty, Autonomous University of Barcelona, 08193 Bellaterra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0295-1307","authenticated-orcid":false,"given":"Blanca","family":"Xicoy","sequence":"additional","affiliation":[{"name":"Hematology Service, Catalan Institute of Oncology-Germans Trias i Pujol Hospital, Josep Carreras Leukaemia Research Institute, Autonomous University of Barcelona, 08025 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1713-7110","authenticated-orcid":false,"given":"Lurdes","family":"Zamora","sequence":"additional","affiliation":[{"name":"Hematology Service, Catalan Institute of Oncology-Germans Trias i Pujol Hospital, Josep Carreras Leukaemia Research Institute, Autonomous University of Barcelona, 08025 Barcelona, Spain"},{"name":"Josep Carreras Leukaemia Research Institute, 08916 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1666-1300","authenticated-orcid":false,"given":"Marta","family":"Vilaseca","sequence":"additional","affiliation":[{"name":"Institute for Research in Biomedicine (IRB Barcelona), 08028 Barcelona, Spain"},{"name":"The Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6206-6481","authenticated-orcid":false,"given":"Alicia","family":"Roque","sequence":"additional","affiliation":[{"name":"Biochemistry and Molecular Biology Department, Biosciences Faculty, Autonomous University of Barcelona, 08193 Bellaterra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1016\/j.bbagrm.2015.10.013","article-title":"Specificities and Genomic Distribution of Somatic Mammalian Histone H1 Subtypes","volume":"1859","author":"Jordan","year":"2016","journal-title":"Biochim. 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