{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:02:59Z","timestamp":1792803779110,"version":"4.5.0"},"reference-count":57,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T00:00:00Z","timestamp":1728000000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Korea Health Technology R&amp;D Project through the Korea Health Industry Development Institute (KHIDI)","award":["HR20C0026"],"award-info":[{"award-number":["HR20C0026"]}],"id":[{"id":"https:\/\/ror.org\/00fdzyk40","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Korea Health Technology R&amp;D Project through the Korea Health Industry Development Institute (KHIDI)","award":["NRF-2021R1I1A3061377"],"award-info":[{"award-number":["NRF-2021R1I1A3061377"]}],"id":[{"id":"https:\/\/ror.org\/00fdzyk40","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Research Foundation of Korea (NRF) grant funded by the Korea government","award":["HR20C0026"],"award-info":[{"award-number":["HR20C0026"]}],"id":[{"id":"https:\/\/ror.org\/013aysd81","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Research Foundation of Korea (NRF) grant funded by the Korea government","award":["NRF-2021R1I1A3061377"],"award-info":[{"award-number":["NRF-2021R1I1A3061377"]}],"id":[{"id":"https:\/\/ror.org\/013aysd81","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Platelets are essential blood components that maintain hemostasis, prevent excessive bleeding, and facilitate wound healing. Reduced platelet counts are implicated in various diseases, including leukemia, hepatitis, cancer, and Alzheimer\u2019s disease. Enhancing megakaryocytic differentiation is a promising strategy to increase platelet production. Compound K (CK), a major bioactive metabolite of ginsenosides from Panax ginseng, has demonstrated anti-cancer and neuroprotective properties. In this study, we investigated the effects of CK on megakaryocytic differentiation and apoptosis in chronic myeloid leukemia (CML) cell lines K562 and Meg-01. CK treatment significantly upregulated the mRNA expression of key megakaryocytic differentiation markers, including CD61, CD41, and CD42a, and promoted the formation of large, multinucleated cells in K562 cells. Additionally, flow cytometry analysis revealed that CK at 5 \u00b5M induced apoptosis, a critical process in thrombocytopoiesis, in both K562 and Meg-01 cells. RT2 Profiler PCR array analysis further identified a marked increase in the expression of genes associated with the activation of the NLRP3 inflammasome in CK-treated K562 and Meg-01 cells. This study is the first to demonstrate that CK promotes megakaryocytic differentiation and apoptosis through the activation of the ERK\/EGR1 and NLRP3 inflammasome pathways. These findings suggest that CK may enhance platelet production, indicating its potential as a therapeutic candidate for platelet-related disorders and other associated diseases.<\/jats:p>","DOI":"10.3390\/biom14101257","type":"journal-article","created":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T10:20:52Z","timestamp":1728037252000},"page":"1257","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Compound K Promotes Megakaryocytic Differentiation by NLRP3 Inflammasome Activation"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8943-1840","authenticated-orcid":false,"given":"Seonhwa","family":"Hwang","sequence":"first","affiliation":[{"name":"College of Pharmacy, Kyungsung University, 309 Suyeong-ro, Busan 48434, Republic of Korea"},{"name":"Brain Busan 21 Plus Research Project Group, Kyungsung University, Busan 48434, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-Seo","family":"Park","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Kyungsung University, 309 Suyeong-ro, Busan 48434, Republic of Korea"},{"name":"Brain Busan 21 Plus Research Project Group, Kyungsung University, Busan 48434, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-4252-3524","authenticated-orcid":false,"given":"Anthony Junhoe","family":"Koo","sequence":"additional","affiliation":[{"name":"College of Engineering, North Carolina A&T State University, Greensboro, NC 27411, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1085-8751","authenticated-orcid":false,"given":"Eunsoo","family":"Yoo","sequence":"additional","affiliation":[{"name":"Chemical, Biological, and Bioengineering Department, North Carolina A&T State University, Greensboro, NC 27411, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seh-Hyon","family":"Song","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Kyungsung University, 309 Suyeong-ro, Busan 48434, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye-Kyung","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Kyungsung University, 309 Suyeong-ro, Busan 48434, Republic of Korea"},{"name":"Brain Busan 21 Plus Research Project Group, Kyungsung University, Busan 48434, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-Hi","family":"Park","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Kyungsung University, 309 Suyeong-ro, Busan 48434, Republic of Korea"},{"name":"Brain Busan 21 Plus Research Project Group, Kyungsung University, Busan 48434, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae-Seon","family":"Kang","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Kyungsung University, 309 Suyeong-ro, Busan 48434, Republic of Korea"},{"name":"Brain Busan 21 Plus Research Project Group, Kyungsung University, Busan 48434, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1126\/science.328.5978.562","article-title":"Cell biology. 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