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Cell signalling, which is essential to cardiac development, also presents potential disease-modifying opportunities to recover and limit myocardial injury or maladaptive remodelling. Here, we hypothesized that Yap signalling could be sensitive to one or more molecular stress patterns associated with early acute ischemia. We found that Yap, and not Taz, expression patterns differed in a post-myocardial infarct compared to a peri-infarct region of rat hearts post-myocardial infarction, suggesting cell specificity that would be challenging to resolve for causation in vivo. Using H9c2 ventricular myotubes in vitro as a model, Yap levels were determined to be more sensitive to nutrient deprivation than other stress patterns typified by ischemia within the first hour of stress. Moreover, this is mediated by amino acid availability, predominantly L-isoleucine, and influences the expression of connective tissue growth factor (Ctgf)\u2014a major determinant of myocardial adaptation after injury. These findings present novel opportunities for future therapeutic development and risk assessment for myocardial injury and adaptation.<\/jats:p>","DOI":"10.3390\/biom14101299","type":"journal-article","created":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T04:02:58Z","timestamp":1728878578000},"page":"1299","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Yap Is a Nutrient Sensor Sensitive to the Amino Acid L-Isoleucine and Regulates the Expression of Ctgf in Cardiomyocytes"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-9781-8468","authenticated-orcid":false,"given":"Victoria L.","family":"Nelson","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Dalhousie University, Halifax, NS B3H 4R2, Canada"},{"name":"Dalhousie Medicine New Brunswick, Faculty of Medicine, Dalhousie University, Saint John, NB E2L 4L5, Canada"},{"name":"IMPART Investigator Team Canada, Saint John, NB E2L 4L5, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9576-6989","authenticated-orcid":false,"given":"Ashley L.","family":"Eadie","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Dalhousie University, Halifax, NS B3H 4R2, Canada"},{"name":"Dalhousie Medicine New Brunswick, Faculty of Medicine, Dalhousie University, Saint John, NB E2L 4L5, Canada"},{"name":"IMPART Investigator Team Canada, Saint John, NB E2L 4L5, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5717-5181","authenticated-orcid":false,"given":"Lester","family":"Perez","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Dalhousie University, Halifax, NS B3H 4R2, Canada"},{"name":"Dalhousie Medicine New Brunswick, Faculty of Medicine, Dalhousie University, Saint John, NB E2L 4L5, Canada"},{"name":"IMPART Investigator Team Canada, Saint John, NB E2L 4L5, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malav","family":"Madhu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Dalhousie University, Halifax, NS B3H 4R2, Canada"},{"name":"Dalhousie Medicine New Brunswick, Faculty of Medicine, Dalhousie University, Saint John, NB E2L 4L5, Canada"},{"name":"IMPART Investigator Team Canada, Saint John, NB E2L 4L5, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathew","family":"Platt","sequence":"additional","affiliation":[{"name":"IMPART Investigator Team Canada, Saint John, NB E2L 4L5, Canada"},{"name":"Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angella","family":"Mercer","sequence":"additional","affiliation":[{"name":"Dalhousie Medicine New Brunswick, Faculty of Medicine, Dalhousie University, Saint John, NB E2L 4L5, Canada"},{"name":"IMPART Investigator Team Canada, Saint John, NB E2L 4L5, Canada"},{"name":"Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS B3H 4R2, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1228-893X","authenticated-orcid":false,"given":"Thomas","family":"Pulinilkunnil","sequence":"additional","affiliation":[{"name":"Dalhousie Medicine New Brunswick, Faculty of Medicine, Dalhousie University, Saint John, NB E2L 4L5, Canada"},{"name":"IMPART Investigator Team Canada, Saint John, NB E2L 4L5, Canada"},{"name":"Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS B3H 4R2, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9643-8338","authenticated-orcid":false,"given":"Petra","family":"Kienesberger","sequence":"additional","affiliation":[{"name":"Dalhousie Medicine New Brunswick, Faculty of Medicine, Dalhousie University, Saint John, NB E2L 4L5, Canada"},{"name":"IMPART Investigator Team Canada, Saint John, NB E2L 4L5, Canada"},{"name":"Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS B3H 4R2, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy A.","family":"Simpson","sequence":"additional","affiliation":[{"name":"IMPART Investigator Team Canada, Saint John, NB E2L 4L5, Canada"},{"name":"Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5728-8135","authenticated-orcid":false,"given":"Keith R.","family":"Brunt","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Dalhousie University, Halifax, NS B3H 4R2, Canada"},{"name":"Dalhousie Medicine New Brunswick, Faculty of Medicine, Dalhousie University, Saint John, NB E2L 4L5, Canada"},{"name":"IMPART Investigator Team 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