{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T21:33:18Z","timestamp":1793568798151,"version":"4.5.0"},"reference-count":45,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,10,16]],"date-time":"2024-10-16T00:00:00Z","timestamp":1729036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Australian Commonwealth Research Training Program Stipend Scholarship","award":["GNT2018194"],"award-info":[{"award-number":["GNT2018194"]}]},{"name":"Australian Commonwealth Research Training Program Stipend Scholarship","award":["GNT2005791"],"award-info":[{"award-number":["GNT2005791"]}]},{"name":"Australian Commonwealth Research Training Program Stipend Scholarship","award":["H21\/174585"],"award-info":[{"award-number":["H21\/174585"]}]},{"name":"National Health and Medical Research Council Australia","award":["GNT2018194"],"award-info":[{"award-number":["GNT2018194"]}]},{"name":"National Health and Medical Research Council Australia","award":["GNT2005791"],"award-info":[{"award-number":["GNT2005791"]}]},{"name":"National Health and Medical Research Council Australia","award":["H21\/174585"],"award-info":[{"award-number":["H21\/174585"]}]},{"name":"NSW Office of Health and Medical Research","award":["GNT2018194"],"award-info":[{"award-number":["GNT2018194"]}]},{"name":"NSW Office of Health and Medical Research","award":["GNT2005791"],"award-info":[{"award-number":["GNT2005791"]}]},{"name":"NSW Office of Health and Medical Research","award":["H21\/174585"],"award-info":[{"award-number":["H21\/174585"]}]},{"name":"Ramsay Teaching and Research Foundation","award":["GNT2018194"],"award-info":[{"award-number":["GNT2018194"]}]},{"name":"Ramsay Teaching and Research Foundation","award":["GNT2005791"],"award-info":[{"award-number":["GNT2005791"]}]},{"name":"Ramsay Teaching and Research Foundation","award":["H21\/174585"],"award-info":[{"award-number":["H21\/174585"]}]},{"name":"BioPlatforms Australia","award":["GNT2018194"],"award-info":[{"award-number":["GNT2018194"]}]},{"name":"BioPlatforms Australia","award":["GNT2005791"],"award-info":[{"award-number":["GNT2005791"]}]},{"name":"BioPlatforms Australia","award":["H21\/174585"],"award-info":[{"award-number":["H21\/174585"]}]},{"name":"Vonwiller Foundation","award":["GNT2018194"],"award-info":[{"award-number":["GNT2018194"]}]},{"name":"Vonwiller Foundation","award":["GNT2005791"],"award-info":[{"award-number":["GNT2005791"]}]},{"name":"Vonwiller Foundation","award":["H21\/174585"],"award-info":[{"award-number":["H21\/174585"]}]},{"name":"Heart Research Australia","award":["GNT2018194"],"award-info":[{"award-number":["GNT2018194"]}]},{"name":"Heart Research Australia","award":["GNT2005791"],"award-info":[{"award-number":["GNT2005791"]}]},{"name":"Heart Research Australia","award":["H21\/174585"],"award-info":[{"award-number":["H21\/174585"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Background and Aims: Myeloperoxidase (MPO) plays a critical role in the innate immune response and has been suggested to be a surrogate marker of oxidative stress and inflammation, with elevated levels implicated in cardiovascular diseases, such as atherosclerosis and heart failure, as well as in conditions like rheumatoid arthritis and cancer. While MPO is well-known in leukocytes, its expression and function in human endothelial cells remain unclear. This study investigates MPO expression in patient-derived endothelial colony-forming cells (ECFCs) and its potential association with CAD and mitochondrial function. Methods: ECFCs were cultured from the peripheral blood of 93 BioHEART-CT patients. MPO expression and associated functions were examined using qRT-PCR, immunochemistry, flow cytometry, and MPO activity assays. CAD presence was defined using CT coronary angiography (CACS &gt; 0). Results: We report MPO presence in patient-derived ECFCs for the first time. MPO protein expression occurred in 70.7% of samples (n = 41) which had nuclear co-localisation, an atypical observation given its conventional localisation in the granules of neutrophils and monocytes. This suggests potential alternative roles for MPO in nuclear processes. MPO mRNA expression was detected in 66.23% of samples (n = 77). CAD patients had a lower proportion of MPO-positive ECFCs compared to non-CAD controls (57.45% vs. 80%, p = 0.04), a difference that persisted in the statin-na\u00efve sub-cohort (53.85% vs. 84.62%, p = 0.02). Non-CAD patients with MPO expression showed upregulated mitochondrial-antioxidant genes (AIFM2, TXNRD1, CAT, PRDX3, PRDX6). In contrast, CAD patients with MPO gene expression had heightened mROS production and mitochondrial mass and decreased mitochondrial function compared to that of CAD patients without MPO gene expression. Conclusions: MPO is present in the nucleus of ECFCs. In non-CAD ECFCs, MPO expression is linked to upregulated mitochondrial-antioxidant genes, whereas in CAD ECFCs, it is associated with greater mitochondrial dysfunction.<\/jats:p>","DOI":"10.3390\/biom14101308","type":"journal-article","created":{"date-parts":[[2024,10,18]],"date-time":"2024-10-18T09:14:48Z","timestamp":1729242888000},"page":"1308","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Expression of Myeloperoxidase in Patient-Derived Endothelial Colony-Forming Cells\u2014Associations with Coronary Artery Disease and Mitochondrial Function"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2232-1759","authenticated-orcid":false,"given":"Weiqian Eugene","family":"Lee","sequence":"first","affiliation":[{"name":"Kolling Institute, 10 Westbourne Street, St Leonards, Sydney, NSW 2064, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elijah","family":"Genetzakis","sequence":"additional","affiliation":[{"name":"Kolling Institute, 10 Westbourne Street, St Leonards, Sydney, NSW 2064, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giannie","family":"Barsha","sequence":"additional","affiliation":[{"name":"The Victorian Heart Institute and Biomedicine Discovery Institute, Monash University, Wellington Road, Clayton, Melbourne, VIC 3800, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0845-6204","authenticated-orcid":false,"given":"Joshua","family":"Vescovi","sequence":"additional","affiliation":[{"name":"Kolling Institute, 10 Westbourne Street, St Leonards, Sydney, NSW 2064, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7932-5400","authenticated-orcid":false,"given":"Carmen","family":"Mifsud","sequence":"additional","affiliation":[{"name":"Kolling Institute, 10 Westbourne Street, St Leonards, Sydney, NSW 2064, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen T.","family":"Vernon","sequence":"additional","affiliation":[{"name":"Kolling Institute, 10 Westbourne Street, St Leonards, Sydney, NSW 2064, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1294-7739","authenticated-orcid":false,"given":"Tung Viet","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Kolling Institute, 10 Westbourne Street, St Leonards, Sydney, NSW 2064, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1154-3011","authenticated-orcid":false,"given":"Michael P.","family":"Gray","sequence":"additional","affiliation":[{"name":"Kolling Institute, 10 Westbourne Street, St Leonards, Sydney, NSW 2064, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2712-157X","authenticated-orcid":false,"given":"Stuart M.","family":"Grieve","sequence":"additional","affiliation":[{"name":"Charles Perkins Centre, Johns Hopkins Drive, Camperdown, Sydney, NSW 2050, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gemma A.","family":"Figtree","sequence":"additional","affiliation":[{"name":"Kolling Institute, 10 Westbourne Street, St Leonards, Sydney, NSW 2064, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,16]]},"reference":[{"key":"ref_1","unstructured":"(2023, August 31). 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