{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T04:31:58Z","timestamp":1789187518928,"version":"build-2803163510"},"reference-count":71,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T00:00:00Z","timestamp":1730937600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Korea Basic Science Institute (National Research Facilities and Equipment Center)","award":["2021R1A6C101A416"],"award-info":[{"award-number":["2021R1A6C101A416"]}]},{"name":"Korea Basic Science Institute (National Research Facilities and Equipment Center)","award":["RS-2023-00245922"],"award-info":[{"award-number":["RS-2023-00245922"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["2021R1A6C101A416"],"award-info":[{"award-number":["2021R1A6C101A416"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["RS-2023-00245922"],"award-info":[{"award-number":["RS-2023-00245922"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Nitrogen fixation in legume nodules is crucial for plant growth and development. Therefore, this study aims to investigate the effects of nitric oxide [S-nitrosoglutathione (GSNO)] and silicon [sodium metasilicate (Si)], both individually and in combination, on soybean growth, nodule formation, leghaemoglobin (Lb) synthesis, and potential post-translational modifications. At the V1 stage, soybean plants were treated for 2 weeks with 150 \u00b5M GSNO, and Si at concentrations of 1 mM, 2 mM, and 4 mM. The results showed that NO and Si enhance the nodulation process by increasing phenylalanine ammonia-lyase activity and Nod factors (NIP2-1), attracting rhizobia and accelerating nodule formation. This leads to a greater number and larger diameter of nodules. Individually, NO and Si support the synthesis of Lb and leghaemoglobin protein (Lba) expression, ferric leghaemoglobin reductases (FLbRs), and S-nitrosoglutathione reductase (GSNOR). However, when used in combination, NO and Si inhibit these processes, leading to elevated levels of S-nitrosothiols in the roots and nodules. This combined inhibition may potentially induce post-translational modifications in FLbRs, pivotal for the reduction of Lb3+ to Lb2+. These findings underscore the critical role of NO and Si in the nodulation process and provide insight into their combined effects on this essential plant function.<\/jats:p>","DOI":"10.3390\/biom14111417","type":"journal-article","created":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T04:22:56Z","timestamp":1730953376000},"page":"1417","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Interaction Between Nitric Oxide and Silicon on Leghaemoglobin and S-Nitrosothiol Levels in Soybean Nodules"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-7045-4619","authenticated-orcid":false,"given":"Da-Sol","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 41566, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6012-4812","authenticated-orcid":false,"given":"Ashim Kumar","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 41566, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4108-3028","authenticated-orcid":false,"given":"Nusrat Jahan","family":"Methela","sequence":"additional","affiliation":[{"name":"Department of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 41566, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5451-2365","authenticated-orcid":false,"given":"Byung-Wook","family":"Yun","sequence":"additional","affiliation":[{"name":"Department of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 41566, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1007\/s13165-022-00403-4","article-title":"Up-scaling organic agriculture to enhance food and water security in South Asia","volume":"12","author":"Das","year":"2022","journal-title":"Org. 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