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It is characterized by cognitive impairment and dementia due to abnormal levels of amyloid beta peptide (A\u03b2) and axonal Tau protein in the brain. However, the complex underlying mechanisms affecting this disease are not yet known, and there is a lack of standardized biomarkers and therapeutic targets. Therefore, in this study, by means of bioinformatics analysis, AD-affected brain tissue was analyzed using the GSE138260 dataset, identifying 612 differentially expressed genes (DEGs). Functional analysis revealed 388 upregulated DEGs associated with sensory perception and 224 downregulated DEGs linked to the regulation and modulation of synaptic processes. Protein\u2013protein interaction network analysis identified 20 hub genes. Furthermore, miRNA target gene networks revealed 1767 miRNAs linked to hub genes, among which hsa-mir-106a-5p, hsa-mir-17-5p, hsa-mir-26a-5p, hsa-mir-27a-3p and hsa-mir-34a-5p were the most relevant. This study presents novel biomarkers and therapeutic targets for AD by analyzing the information obtained with a comprehensive literature review, providing new potential targets to study their role in AD.<\/jats:p>","DOI":"10.3390\/biom14121641","type":"journal-article","created":{"date-parts":[[2024,12,23]],"date-time":"2024-12-23T04:33:29Z","timestamp":1734928409000},"page":"1641","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Identifying Hub Genes and miRNAs Associated with Alzheimer\u2019s Disease: A Bioinformatics Pathway to Novel Therapeutic Strategies"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-3380-0465","authenticated-orcid":false,"given":"Elisa","family":"Gasc\u00f3n","sequence":"first","affiliation":[{"name":"Department of Anatomy, Embryology and Animal Genetics, University of Zaragoza, 50013 Zaragoza, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Neurodegenerativas (CIBERNED), Av. Monforte de Lemos 3-5, 28029 Madrid, Spain"},{"name":"Agroalimentary Institute of Aragon (IA2), University of Zaragoza, 50013 Zaragoza, Spain"},{"name":"Institute of Health Research of Aragon (IIS), Av. San Juan Bosco 13, 50009 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5193-7782","authenticated-orcid":false,"given":"Ana Cristina","family":"Calvo","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Embryology and Animal Genetics, University of Zaragoza, 50013 Zaragoza, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Neurodegenerativas (CIBERNED), Av. Monforte de Lemos 3-5, 28029 Madrid, Spain"},{"name":"Agroalimentary Institute of Aragon (IA2), University of Zaragoza, 50013 Zaragoza, Spain"},{"name":"Institute of Health Research of Aragon (IIS), Av. San Juan Bosco 13, 50009 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0615-3043","authenticated-orcid":false,"given":"Nora","family":"Molina","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Embryology and Animal Genetics, University of Zaragoza, 50013 Zaragoza, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Neurodegenerativas (CIBERNED), Av. Monforte de Lemos 3-5, 28029 Madrid, Spain"},{"name":"Agroalimentary Institute of Aragon (IA2), University of Zaragoza, 50013 Zaragoza, Spain"},{"name":"Institute of Health Research of Aragon (IIS), Av. 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