{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T08:38:18Z","timestamp":1787647098872,"version":"build-2736575974"},"reference-count":59,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T00:00:00Z","timestamp":1735516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Cancer Center Research Grant","award":["NCC-2210470"],"award-info":[{"award-number":["NCC-2210470"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Metastatic cancer accounts for most cancer-related deaths, and identifying specific molecular targets that contribute to metastatic progression is crucial for the development of effective treatments. Hypoxia, a feature of solid tumors, plays a role in cancer progression by inducing resistance to therapy and accelerating metastasis. Here, we report that CCAAT\/enhancer-binding protein beta (C\/EBP\u03b2) transcriptionally regulates hypoxia-inducible factor 1 subunit alpha (HIF1A) and thus promotes migration and invasion of non-small-cell lung cancer (NSCLC) cells under hypoxic conditions. Our results show that knockdown or forced expression of C\/EBP\u03b2 was correlated with HIF-1\u03b1 expression and that C\/EBP\u03b2 directly bound to the promoter region of HIF1A. Silencing HIF1A inhibited the enhanced migration and invasion induced by C\/EBP\u03b2 overexpression in NSCLC cells under hypoxia. Expression of the HIF-1\u03b1 target gene, SLC2A1, was also altered in a C\/EBP\u03b2-dependent manner, and knockdown of SLC2A1 reduced migration and invasion enhanced by C\/EBP\u03b2 overexpression. These results indicate that C\/EBP\u03b2 is a critical regulator for the invasion of NSCLC cells in the hypoxic tumor microenvironment. Collectively, the C\/EBP\u03b2-HIF-1\u03b1-GLUT1 axis represents a potential therapeutic target for preventing metastatic progression of NSCLC and improving patient outcomes.<\/jats:p>","DOI":"10.3390\/biom15010036","type":"journal-article","created":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T13:26:25Z","timestamp":1735651585000},"page":"36","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["C\/EBP\u03b2 Regulates HIF-1\u03b1-Driven Invasion of Non-Small-Cell Lung Cancer Cells"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7755-7363","authenticated-orcid":false,"given":"Seung Hee","family":"Seo","sequence":"first","affiliation":[{"name":"Cancer Metastasis Branch, Research Institute, National Cancer Center, Goyang 10408, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Hae","family":"Lee","sequence":"additional","affiliation":[{"name":"Cancer Metastasis Branch, Research Institute, National Cancer Center, Goyang 10408, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun Kyung","family":"Choi","sequence":"additional","affiliation":[{"name":"Cancer Metastasis Branch, Research Institute, National Cancer Center, Goyang 10408, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung Bae","family":"Rho","sequence":"additional","affiliation":[{"name":"Cancer Metastasis Branch, Research Institute, National Cancer Center, Goyang 10408, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyungsil","family":"Yoon","sequence":"additional","affiliation":[{"name":"Cancer Metastasis Branch, Research Institute, National Cancer Center, Goyang 10408, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"17","DOI":"10.3322\/caac.21763","article-title":"Cancer statistics, 2023","volume":"73","author":"Siegel","year":"2023","journal-title":"CA Cancer J. 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