{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T17:31:07Z","timestamp":1793035867786,"version":"4.5.0"},"reference-count":93,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T00:00:00Z","timestamp":1742169600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["20-74-10072"],"award-info":[{"award-number":["20-74-10072"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Human serum albumin (HSA) is a natural depot of amyloid-\u03b2 peptide (A\u03b2), a key player in Alzheimer\u2019s disease (AD). HSA and pro-inflammatory Ca2+-binding proteins S100A8 and S100A9 are involved in A\u03b2 metabolism and its deposition in the brain, serving as probable triggers and therapeutic targets in AD, but their interplay with regard to A\u03b2 binding\/fibrillation is unclear. To this end, here we explore the in vitro binding of Ca2+-bound S100A8 or S100A9 to monomeric A\u03b2 and the influence of the S100 proteins on A\u03b2 fibrillation. The equilibrium dissociation constants of the complexes of dimeric S100A8\/S100A9 with A\u03b240\/42 estimated by biolayer interferometry are 1\u20135 \u00b5M. S100A8 and S100A9 interfere with HSA binding to A\u03b2. Thioflavin T assay and electron microscopy data show that micromolar S100A8\/S100A9 inhibit A\u03b240 fibrillation, and the inhibitory effect of S100A8 exceeds that for HSA. The competition for A\u03b2 between HSA and S100A8\/S100A9 may contribute to the A\u03b2-HSA imbalance in the pro-inflammatory conditions in AD.<\/jats:p>","DOI":"10.3390\/biom15030431","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T15:24:56Z","timestamp":1742225096000},"page":"431","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Binding of Pro-Inflammatory Proteins S100A8 or S100A9 to Amyloid-\u03b2 Peptide Suppresses Its Fibrillation"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7459-6295","authenticated-orcid":false,"given":"Ekaterina A.","family":"Litus","sequence":"first","affiliation":[{"name":"Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Institutskaya Str., 7, Pushchino, 142290 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7638-5143","authenticated-orcid":false,"given":"Marina P.","family":"Shevelyova","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Institutskaya Str., 7, Pushchino, 142290 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5550-2129","authenticated-orcid":false,"given":"Alisa A.","family":"Vologzhannikova","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Institutskaya Str., 7, Pushchino, 142290 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgenia I.","family":"Deryusheva","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Institutskaya Str., 7, Pushchino, 142290 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4859-7219","authenticated-orcid":false,"given":"Andrey V.","family":"Machulin","sequence":"additional","affiliation":[{"name":"Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, pr. Nauki, 5, Pushchino, 142290 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina L.","family":"Nemashkalova","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Institutskaya Str., 7, Pushchino, 142290 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria E.","family":"Permyakova","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Institutskaya Str., 7, Pushchino, 142290 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey S.","family":"Sokolov","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Institutskaya Str., 7, Pushchino, 142290 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria D.","family":"Alikova","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Institutskaya Str., 7, Pushchino, 142290 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4037-5857","authenticated-orcid":false,"given":"Vladimir N.","family":"Uversky","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and USF Health Byrd Alzheimer\u2019s Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4086-3137","authenticated-orcid":false,"given":"Sergei E.","family":"Permyakov","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Institutskaya Str., 7, Pushchino, 142290 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1016\/j.bcp.2013.12.024","article-title":"Alzheimer disease: Epidemiology, diagnostic criteria, risk factors and biomarkers","volume":"88","author":"Reitz","year":"2014","journal-title":"Biochem. 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