{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:24:23Z","timestamp":1783297463691,"version":"3.54.6"},"reference-count":32,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T00:00:00Z","timestamp":1747008000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Science Foundation of Fujian Province, China","award":["2021J01892"],"award-info":[{"award-number":["2021J01892"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>For the first time, two poly-histidine-poly-glycine peptides (pHpG-H5 and pHpG-H7) were identified as promising candidates for matrix metalloproteinase inhibitors. cDNAs encoding pHpG-H5 and pHpG-H7 peptides were isolated from the Atheris squamigera cDNA library constructed using oligo(dT)-primed reverse transcription. Deduced sequences of pHpG peptides were systematically organised and utilised as templates for synthesising chemical replicates. All synthetic pHpG peptides exhibited inhibitory effects on human matrix metalloproteinase-1 (MMP-1). Spectroscopic analyses and molecular modelling demonstrated that pHpG peptides disrupt zinc ion coordination within the central catalytic domain of MMP-1, thereby inhibiting its enzymatic activity. As a novel peptide inhibitor of matrix metalloproteinase, pHpG-H7 modulates multiple biological processes, such as cell migration and angiogenesis, suggesting significant therapeutic potential.<\/jats:p>","DOI":"10.3390\/biom15050706","type":"journal-article","created":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T03:59:26Z","timestamp":1747108766000},"page":"706","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Discovery and Characterisation of Novel Poly-Histidine-Poly-Glycine Peptides as Matrix Metalloproteinase Inhibitors"],"prefix":"10.3390","volume":"15","author":[{"given":"He","family":"Wang","sequence":"first","affiliation":[{"name":"School of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenchao","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Bioengineering and Biopharmaceutics, School of Pharmacy, Fujian Medical University, Fuzhou 350000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyu","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Bioengineering and Biopharmaceutics, School of Pharmacy, Fujian Medical University, Fuzhou 350000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juanli","family":"Fu","sequence":"additional","affiliation":[{"name":"School of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1560-5948","authenticated-orcid":false,"given":"Enrico","family":"K\u00f6nig","sequence":"additional","affiliation":[{"name":"Laboratory of Synthetic and Structural Vaccinology, University of Trento, 38100 Trento, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6711-1560","authenticated-orcid":false,"given":"Nanwen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Bioengineering and Biopharmaceutics, School of Pharmacy, Fujian Medical University, Fuzhou 350000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9986-5044","authenticated-orcid":false,"given":"Xiaole","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Bioengineering and Biopharmaceutics, School of Pharmacy, Fujian Medical University, Fuzhou 350000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8011-9260","authenticated-orcid":false,"given":"Tianbao","family":"Chen","sequence":"additional","affiliation":[{"name":"Medicine Natural Peptide Discovery Group, School of Pharmacy, Queen\u2019s University Belfast, Belfast BT7 1NN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Shaw","sequence":"additional","affiliation":[{"name":"Medicine Natural Peptide Discovery Group, School of Pharmacy, Queen\u2019s University Belfast, Belfast BT7 1NN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2018","DOI":"10.1016\/j.toxrep.2022.11.003","article-title":"Envenomation by the Green Bush Viper Atheris squamigera","volume":"9","author":"Ontiveros","year":"2022","journal-title":"Toxicol. 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