{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T16:44:18Z","timestamp":1790441058776,"version":"4.1.0"},"reference-count":55,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T00:00:00Z","timestamp":1747008000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010889","name":"OmniActive Health Technologies","doi-asserted-by":"publisher","award":["2024-1"],"award-info":[{"award-number":["2024-1"]}],"id":[{"id":"10.13039\/100010889","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010889","name":"OmniActive Health Technologies","doi-asserted-by":"publisher","award":["2024-2"],"award-info":[{"award-number":["2024-2"]}],"id":[{"id":"10.13039\/100010889","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004412","name":"Turkish Academy of Sciences","doi-asserted-by":"publisher","award":["2024-1"],"award-info":[{"award-number":["2024-1"]}],"id":[{"id":"10.13039\/501100004412","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004412","name":"Turkish Academy of Sciences","doi-asserted-by":"publisher","award":["2024-2"],"award-info":[{"award-number":["2024-2"]}],"id":[{"id":"10.13039\/501100004412","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Ashwagandha (Withania somnifera) is a well-known adaptogenic herb traditionally used to enhance sleep quality and mitigate stress-induced cognitive decline. This study investigated the effects of different doses of ashwagandha root extract (AE) formulations on cognitive function, oxidative stress, and neuronal plasticity in a rat model of sleep deprivation (SD). Forty-nine rats were randomly assigned to seven groups: control, wide platform (WP), SD, SD + A1 (15 mg\/kg AE 1.5%), SD + A2 (30 mg\/kg AE 1.5%), SD + A3 (5.5 mg\/kg AE 8.0%), and SD + A4 (11 mg\/kg AE 8.0%). The extract was administered orally for four weeks. SD induced via a modified wide platform model significantly impaired spatial memory, increased oxidative stress, and suppressed GABA receptor activity. Treatment with all AE doses, except 15 mg\/kg AE 1.5%, considerably reduced serum corticosterone (12% for SD + A2, 15% for SD + A3, and 32% for SD + A4), CRH (11% for SD + A2, 14% for SD + A3, and 17% for SD + A4), ACTH (22% for SD + A2, 26% for SD + A3, and 38% for SD + A4), and MDA levels (31% for SD + A2, 34% for SD + A3, and 46% for SD + A4) (p &lt; 0.05). All doses improved antioxidant enzyme activity and memory performance, while AE 8.0% doses notably increased serotonin (19% for SD + A3 and 33% for SD + A4) and dopamine levels (40% for SD + A3 and 50% for SD + A4). Moreover, AE treatment enhanced markers of neuronal plasticity and partially improved GABAergic function. These findings suggest that AE formulations, particularly at higher concentrations, exert neuroprotective effects against SD-induced cognitive impairment by modulating oxidative stress, neurotransmitter balance, and neuroplasticity, indicating their potential application in managing stress-related neurological disorders.<\/jats:p>","DOI":"10.3390\/biom15050710","type":"journal-article","created":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T03:59:26Z","timestamp":1747108766000},"page":"710","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["A Novel Ashwagandha (Withania somnifera) Formulation Mitigates Sleep Deprivation-Induced Cognitive Impairment and Oxidative Stress in a Rat Model"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9583-2218","authenticated-orcid":false,"given":"Besir","family":"Er","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Science, Firat University, Elazig 23119, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0041-9421","authenticated-orcid":false,"given":"Busra","family":"Ozmen","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition, Faculty of Veterinary Medicine, Firat University, Elazig 23119, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7625-1883","authenticated-orcid":false,"given":"Emre","family":"Sahin","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition, Faculty of Veterinary Medicine, Bingol University, Bingol 12100, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4138-7689","authenticated-orcid":false,"given":"Cemal","family":"Orhan","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition, Faculty of Veterinary Medicine, Firat University, Elazig 23119, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurhan","family":"Sahin","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition, Faculty of Veterinary Medicine, Firat University, Elazig 23119, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1575-9071","authenticated-orcid":false,"given":"Abhijeet A.","family":"Morde","sequence":"additional","affiliation":[{"name":"Research and Development, OmniActive Health Technologies Co., Ltd., Mumbai 400013, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0926-6625","authenticated-orcid":false,"given":"Muralidhara","family":"Padigaru","sequence":"additional","affiliation":[{"name":"Research and Development, OmniActive Health Technologies Co., Ltd., Mumbai 400013, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9542-5244","authenticated-orcid":false,"given":"Kazim","family":"Sahin","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition, Faculty of Veterinary Medicine, Firat University, Elazig 23119, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.ccm.2022.02.001","article-title":"Sleep Deficiency: Epidemiology and Effects","volume":"43","author":"Gohari","year":"2022","journal-title":"Clin. 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