{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T13:34:40Z","timestamp":1787664880369,"version":"build-2736575974"},"reference-count":48,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,7,13]],"date-time":"2025-07-13T00:00:00Z","timestamp":1752364800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Program of Hebei University of Environmental Engineering","award":["XJXM-ZD-2024001"],"award-info":[{"award-number":["XJXM-ZD-2024001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>The content of glutathione in the human body is crucial to human health, so a convenient and efficient method is needed to detect it. Herein, porous coordination network-224 (PCN-224) was modified by polydopamine to prepare polydopamine-modified PCN-224 (PCN-224-PDA) to improve the water dispersibility of the PCN-224. Monodispersed platinum nanoparticles were loaded into the PCN-224-PDA to prepare PCN-224-PDA-Pt. The PCN-224-PDA-Pt showed high peroxidase-like catalytic activity, and its catalytic activity was affected by pH and temperature. The PCN-224-PDA-Pt almost had no hemolysis of red blood cells. In addition, the PCN-224-PDA-Pt showed high affinity for 3,3\u2032,5,5\u2032-tetramethylbenzidine and catalytic efficiency in kinetic studies, and the type of reactive oxygen species generated during the catalytic process was hydroxyl radicals. More importantly, a colorimetric method for glutathione detection was developed based on the peroxidase-like activity of the PCN-224-PDA-Pt. The linear detection range was 1\u2013600 \u03bcM and the detection limit reached 0.306 \u03bcM. This method shows good anti-interference capabilities and excellent recovery rates, indicating its strong potential for applications in biological detection.<\/jats:p>","DOI":"10.3390\/biom15071002","type":"journal-article","created":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T07:54:23Z","timestamp":1752479663000},"page":"1002","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Platinum Nanoparticles Loaded in Polydopamine-Modified Porous Coordination Network-224 with Peroxidase-Like Activity for Sensitive Glutathione Detection"],"prefix":"10.3390","volume":"15","author":[{"given":"Shoubei","family":"Gao","sequence":"first","affiliation":[{"name":"Hebei Key Laboratory of Agroecological Safety, Department of Environmental Engineering, Hebei University of Environmental Engineering, Qinhuangdao 066102, China"},{"name":"State Key Laboratory of Metastable Materials Science and Technology, Hebei Key Laboratory of Nano-Biotechnology, Hebei Key Laboratory of Applied Chemistry, Yanshan University, Qinhuangdao 066004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingyue","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Metastable Materials Science and Technology, Hebei Key Laboratory of Nano-Biotechnology, Hebei Key Laboratory of Applied Chemistry, Yanshan University, Qinhuangdao 066004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenran","family":"Zhen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Metastable Materials Science and Technology, Hebei Key Laboratory of Nano-Biotechnology, Hebei Key Laboratory of Applied Chemistry, Yanshan University, Qinhuangdao 066004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanshuai","family":"Cui","sequence":"additional","affiliation":[{"name":"Hebei Key Laboratory of Agroecological Safety, Department of Environmental Engineering, Hebei University of Environmental Engineering, Qinhuangdao 066102, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianbing","family":"Ji","sequence":"additional","affiliation":[{"name":"Hebei Key Laboratory of Agroecological Safety, Department of Environmental Engineering, Hebei University of Environmental Engineering, Qinhuangdao 066102, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruyu","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Metastable Materials Science and Technology, Hebei Key Laboratory of Nano-Biotechnology, Hebei Key Laboratory of Applied Chemistry, Yanshan University, Qinhuangdao 066004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longgang","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Metastable Materials Science and Technology, Hebei Key Laboratory of Nano-Biotechnology, Hebei Key Laboratory of Applied Chemistry, Yanshan University, Qinhuangdao 066004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.ccr.2018.03.021","article-title":"Recent progress on the development of glutathione (GSH) selective fluorescent and colorimetric probes","volume":"366","author":"Lee","year":"2018","journal-title":"Coord. 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