{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:30:34Z","timestamp":1760059834124,"version":"build-2065373602"},"reference-count":45,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T00:00:00Z","timestamp":1752537600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005357","name":"Slovak Research and Development Agency","doi-asserted-by":"publisher","award":["APVV-14-0348","APVV-18-0146","VEGA 1\/0160\/18","VEGA 1\/0011\/23"],"award-info":[{"award-number":["APVV-14-0348","APVV-18-0146","VEGA 1\/0160\/18","VEGA 1\/0011\/23"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Scientific Grant Agency of the Ministry of Education, Science, Research, and Sport of the Slovak Republic and Slovak Academy of Sciences","award":["APVV-14-0348","APVV-18-0146","VEGA 1\/0160\/18","VEGA 1\/0011\/23"],"award-info":[{"award-number":["APVV-14-0348","APVV-18-0146","VEGA 1\/0160\/18","VEGA 1\/0011\/23"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>The rabbit is a widely used experimental model for human translational research and stem cell therapy. Many studies have focused on rabbit mesenchymal stem cells from different biological sources for their possible application in regenerative medicine. However, a minimal number of studies have been published aimed at rabbit hematopoietic stem\/progenitor cells, mainly due to the lack of specific anti-rabbit CD34 antibodies. In general, CD34 antigen is commonly used to identify and isolate hematopoietic stem\/progenitor cells in humans and other animal species. The aim of this study was to develop novel monoclonal antibodies highly specific to rabbit CD34 antigen. We used hybridoma technology, two synthetic peptides derived from predicted rabbit CD34 protein, and a recombinant rabbit CD34 protein as immunogens to produce monoclonal antibodies (mAbs) specific to rabbit CD34. The produced antibodies were screened for their binding activity and specificity using ELISA, flow cytometry, and Western blot analysis. Finally, four mAbs (58\/47\/26, 58\/47\/34, 182\/7\/80, and 575\/36\/8) were selected for the final purification process. The purified mAbs recognized up to 2\u20133% of total rabbit bone marrow cells, while about 2% of those cells exhibited CD45 expression, which are likely rabbit primitive hematopoietic stem cells and their hematopoietic progenitors, respectively. The newly generated and purified mAbs specifically recognize CD34 antigen in rabbit bone marrow or peripheral blood and can be therefore used for further immunological applications, to study rabbit hematopoiesis or to establish a new animal model for hematopoietic stem cell transplantation studies.<\/jats:p>","DOI":"10.3390\/biom15071021","type":"journal-article","created":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T11:52:58Z","timestamp":1752580378000},"page":"1021","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Generation of Novel Monoclonal Antibodies Recognizing Rabbit CD34 Antigen"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4144-8584","authenticated-orcid":false,"given":"Jarom\u00edr","family":"Va\u0161\u00ed\u010dek","sequence":"first","affiliation":[{"name":"NPPC\u2014Research Institute for Animal Production Nitra, Hlohoveck\u00e1 2, 951 41 Lu\u017eianky, Slovakia"},{"name":"Institute of Biotechnology, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5497-2900","authenticated-orcid":false,"given":"Miroslav","family":"Bauer","sequence":"additional","affiliation":[{"name":"NPPC\u2014Research Institute for Animal Production Nitra, Hlohoveck\u00e1 2, 951 41 Lu\u017eianky, Slovakia"},{"name":"Department of Botany and Genetics, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, N\u00e1bre\u017eie Ml\u00e1de\u017ee 91, 949 74 Nitra, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Kontsekov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Neuroimmunology, Slovak Academy of Sciences, D\u00fabravsk\u00e1 cesta 9, 845 10 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0303-4794","authenticated-orcid":false,"given":"Andrej","family":"Bal\u00e1\u017ei","sequence":"additional","affiliation":[{"name":"NPPC\u2014Research Institute for Animal Production Nitra, Hlohoveck\u00e1 2, 951 41 Lu\u017eianky, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4626-4450","authenticated-orcid":false,"given":"Andrea","family":"Svoradov\u00e1","sequence":"additional","affiliation":[{"name":"NPPC\u2014Research Institute for Animal Production Nitra, Hlohoveck\u00e1 2, 951 41 Lu\u017eianky, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8634-8432","authenticated-orcid":false,"given":"Linda","family":"Duj\u00ed\u010dkov\u00e1","sequence":"additional","affiliation":[{"name":"NPPC\u2014Research Institute for Animal Production Nitra, Hlohoveck\u00e1 2, 951 41 Lu\u017eianky, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2895-1249","authenticated-orcid":false,"given":"Eva","family":"Tvrd\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6770-0077","authenticated-orcid":false,"given":"Jakub","family":"Vozaf","sequence":"additional","affiliation":[{"name":"NPPC\u2014Research Institute for Animal Production Nitra, Hlohoveck\u00e1 2, 951 41 Lu\u017eianky, Slovakia"},{"name":"Institute of Biotechnology, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Supuka","sequence":"additional","affiliation":[{"name":"VETSERVIS Ltd., Kalv\u00e1ria 3, 949 01 Nitra, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4188-1540","authenticated-orcid":false,"given":"Peter","family":"Chrenek","sequence":"additional","affiliation":[{"name":"NPPC\u2014Research Institute for Animal Production Nitra, Hlohoveck\u00e1 2, 951 41 Lu\u017eianky, Slovakia"},{"name":"Institute of Biotechnology, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Mitra, S., and Tomar, P. (2021). Hybridoma technology; advancements, clinical significance, and future aspects. J. Genet. Eng. Biotechnol., 19.","DOI":"10.1186\/s43141-021-00264-6"},{"key":"ref_2","unstructured":"Harlow, E., and Lane, D. (1988). Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"106639","DOI":"10.1016\/j.intimp.2020.106639","article-title":"Hybridoma technology a versatile method for isolation of monoclonal antibodies, its applicability across species, limitations, advancement and future perspectives","volume":"85","author":"Parray","year":"2020","journal-title":"Int. Immunopharmacol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1038\/276269a0","article-title":"Better cell line for making hybridomas secreting specific antibodies","volume":"276","author":"Shulman","year":"1978","journal-title":"Nature"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/256495a0","article-title":"Continuous cultures of fused cells secreting antibody of predefined specificity","volume":"256","author":"Kohler","year":"1975","journal-title":"Nature"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1007\/s12026-015-8663-z","article-title":"A repertoire of high-affinity monoclonal antibodies specific to S-typhi: As potential candidate for improved typhoid diagnostic","volume":"62","author":"Sharma","year":"2015","journal-title":"Immunol. Res."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Betsy, C.J., and Siva, C. (2023). Hybridoma Technology. Fisheries Biotechnology and Bioinformatics, Springer.","DOI":"10.1007\/978-981-99-6991-3_12"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Liu, H., White, J., Crawford, F., Jin, N., Ju, X., Liu, K., Jiang, C., Marrack, P., Zhang, G., and Kappler, J. (2015). A Rapid Method to Characterize Mouse IgG Antibodies and Isolate Native Antigen Binding IgG B Cell Hybridomas. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0136613"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.copbio.2019.01.012","article-title":"Why recombinant antibodies\u2014Benefits and applications","volume":"60","author":"Basu","year":"2019","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.exphem.2020.05.004","article-title":"A sticky wicket: Defining molecular functions for CD34 in hematopoietic cells","volume":"86","author":"Hughes","year":"2020","journal-title":"Exp. Hematol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1084\/jem.183.4.1797","article-title":"Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo","volume":"183","author":"Goodell","year":"1996","journal-title":"J. Exp. Med."},{"key":"ref_12","first-page":"498","article-title":"Bone-marrow culture and transduction of stem-cells in a miniature swine model","volume":"20","author":"Emery","year":"1994","journal-title":"Blood Cells"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1016\/S0093-691X(01)00503-9","article-title":"Evidence against humoral immune attack as the cause of sheep-goat interspecies and hybrid pregnancy failure in the doe","volume":"55","author":"Oppenheim","year":"2001","journal-title":"Theriogenology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"9583","DOI":"10.1073\/pnas.1016394108","article-title":"Platelet gene therapy improves hemostatic function for integrin \u03b1IIb\u03b23-deficient dogs","volume":"108","author":"Fang","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.1182\/blood.V91.6.1977","article-title":"Characterization of monoclonal antibodies that recognize canine CD34","volume":"91","author":"McSweeney","year":"1998","journal-title":"Blood"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.exphem.2006.08.019","article-title":"Development of an anti-porcine CD34 monoclonal antibody that identifies hematopoietic stem cells","volume":"35","author":"Layton","year":"2007","journal-title":"Exp. Hematol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1016\/j.exphem.2008.09.003","article-title":"Development and characterization of a novel CD34 monoclonal antibody that identifies sheep hematopoietic stem\/progenitor cells","volume":"36","author":"Porada","year":"2008","journal-title":"Exp. Hematol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1801","DOI":"10.1093\/molbev\/msr003","article-title":"The Genetic Structure of Domestic Rabbits","volume":"28","author":"Carneiro","year":"2011","journal-title":"Mol. Biol. Evol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Houdebine, L.-M., and Fan, J. (2009). Introduction. Rabbit Biotechnology, Springer.","DOI":"10.1007\/978-90-481-2227-1"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1590\/S0102-86502009000400014","article-title":"The rabbit as an animal model for experimental surgery","volume":"24","author":"Monteiro","year":"2009","journal-title":"Acta Cir. Bras."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"111","DOI":"10.4103\/1735-3327.92960","article-title":"Rabbit as an animal model for experimental research","volume":"9","author":"Mapara","year":"2012","journal-title":"Dent. Res. J."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Houdebine, L.-M., and Fan, J. (2009). Rabbit as a Model for the Study of Human Diseases. Rabbit Biotechnology, Springer.","DOI":"10.1007\/978-90-481-2227-1"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"732","DOI":"10.4103\/ijmr.IJMR_93_19","article-title":"Comparing the chondrogenic potential of rabbit mesenchymal stem cells derived from the infrapatellar fat pad, periosteum & bone marrow","volume":"154","author":"Rajagopal","year":"2021","journal-title":"Indian J. Med. Res."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Kamaruzaman, N.A., Kardia, E., Kamaldin, N.A., Latahir, A.Z., and Yahaya, B.H. (2013). The Rabbit as a Model for Studying Lung Disease and Stem Cell Therapy. BioMed Res. Int., 2013.","DOI":"10.1155\/2013\/691830"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"43","DOI":"10.2147\/SCCAA.S175749","article-title":"Distinct features of rabbit and human adipose-derived mesenchymal stem cells: Implications for biotechnology and translational research","volume":"11","author":"Zomer","year":"2018","journal-title":"Stem Cells Cloning-Adv. Appl."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1002\/btpr.2659","article-title":"Critical Assessment of the Efficiency of CD34 and CD133 Antibodies for Enrichment of Rabbit Hematopoietic Stem Cells","volume":"34","author":"Vasicek","year":"2018","journal-title":"Biotechnol. Prog."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Vasicek, J., Balazi, A., Bauer, M., Svoradova, A., Tirpakova, M., Tomka, M., and Chrenek, P. (2021). Molecular Profiling and Gene Banking of Rabbit EPCs Derived from Two Biological Sources. Genes, 12.","DOI":"10.3390\/genes12030366"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"D523","DOI":"10.1093\/nar\/gkac1052","article-title":"UniProt: The Universal Protein Knowledgebase in 2023","volume":"51","author":"Bateman","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3683","DOI":"10.1242\/jcs.037507","article-title":"Novel functions of the CD34 family","volume":"121","author":"Nielsen","year":"2008","journal-title":"J. Cell Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"W24","DOI":"10.1093\/nar\/gkx346","article-title":"BepiPred-2.0: Improving sequence-based B-cell epitope prediction using conformational epitopes","volume":"45","author":"Jespersen","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"ref_31","first-page":"18","article-title":"The effect of postfusion cell-density on establishment of hybridomas","volume":"34","author":"Kontsekova","year":"1988","journal-title":"Folia Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/0022-1759(91)90333-B","article-title":"One-step method for establishment 8-azaguanine-resistant hybridomas suitable for the preparation of triomas","volume":"145","author":"Kontsekova","year":"1991","journal-title":"J. Immunol. Methods"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.nbt.2019.08.001","article-title":"Combined approach for characterization and quality assessment of rabbit bone marrow-derived mesenchymal stem cells intended for gene banking","volume":"54","author":"Vasicek","year":"2020","journal-title":"New Biotechnol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1380","DOI":"10.1002\/stem.1661","article-title":"Concise Review: Evidence for CD34 as a Common Marker for Diverse Progenitors","volume":"32","author":"Sidney","year":"2014","journal-title":"Stem Cells"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Va\u0161\u00ed\u010dek, J., Bal\u00e1\u017ei, A., Bauer, M., Svoradov\u00e1, A., Tirp\u00e1kov\u00e1, M., Ondru\u0161ka, \u013d., Park\u00e1nyi, V., Makarevich, A.V., and Chrenek, P. (2021). Enrichment of Rabbit Primitive Hematopoietic Cells via MACS Depletion of CD45+ Bone Marrow Cells. Magnetochemistry, 7.","DOI":"10.3390\/magnetochemistry7010011"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Tirpakova, M., Vasicek, J., Svoradova, A., Balazi, A., Tomka, M., Bauer, M., Makarevich, A., and Chrenek, P. (2021). Phenotypical Characterization and Neurogenic Differentiation of Rabbit Adipose Tissue-Derived Mesenchymal Stem Cells. Genes, 12.","DOI":"10.3390\/genes12030431"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"308","DOI":"10.5582\/bst.2014.01084","article-title":"Differentiation ability of multipotent hematopoietic stem\/progenitor cells detected by a porcine specific anti-CD117 monoclonal antibody","volume":"8","author":"Ohshima","year":"2014","journal-title":"Biosci. Trends"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.vetimm.2007.03.010","article-title":"Expression of CD34 mRNA and protein in cattle","volume":"117","author":"Niku","year":"2007","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"157","DOI":"10.4049\/jimmunol.133.1.157","article-title":"Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1A cells","volume":"133","author":"Civin","year":"1984","journal-title":"J. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1182\/blood.V67.3.842.842","article-title":"Monoclonal-antibody 12-8 recognizes a 115-KD molecule present on both unipotent and multipotent hematopoietic colony-forming cells and their precursors","volume":"67","author":"Andrews","year":"1986","journal-title":"Blood"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3774","DOI":"10.1182\/blood.V88.10.3774.bloodjournal88103774","article-title":"Primitive hematopoietic cells in murine bone marrow express the CD34 antigen","volume":"88","author":"Morel","year":"1996","journal-title":"Blood"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2417","DOI":"10.1182\/blood.V75.12.2417.2417","article-title":"Expression of the CD34 gene in vascular endothelial cells","volume":"75","author":"Fina","year":"1990","journal-title":"Blood"},{"key":"ref_43","first-page":"793","article-title":"Structural and partial amino acid sequence analysis of the human hematopoietic progenitor cell antigen CD34","volume":"2","author":"Sutherland","year":"1988","journal-title":"Leukemia"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1016\/j.bbrc.2018.06.100","article-title":"Unfolding of IgG domains detected by non-reducing SDS-PAGE","volume":"503","author":"Kirley","year":"2018","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"112689","DOI":"10.1016\/j.jim.2019.112689","article-title":"A kinetic ELISA to determine the immunoreactive fraction of monoclonal antibodies","volume":"476","author":"Moreno","year":"2020","journal-title":"J. Immunol. 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