{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:41:06Z","timestamp":1760060466384,"version":"build-2065373602"},"reference-count":43,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T00:00:00Z","timestamp":1756339200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006013","name":"United Arab Emirates University UPAR","doi-asserted-by":"publisher","award":["12M159"],"award-info":[{"award-number":["12M159"]}],"id":[{"id":"10.13039\/501100006013","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Li-Kashing Foundation","award":["12M159"],"award-info":[{"award-number":["12M159"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>After spinal cord injury, pathological changes predominantly proceed caudal to the site of injury. To what extent these changes contribute to abnormalities during regeneration is poorly understood. Here, we addressed this question with a low-thoracic compression injury mouse model. The total numbers of immunohistochemically stained neuronal and glial cell types in the lumbar spinal cord were stereologically determined 6 weeks after injury. We also investigated injured mice deficient in close homolog of L1 (CHL1), which had been reported to recover better after injury than their wild-type littermates. We here report that there were no differences between genotypes in uninjured animals. In both injured CHL1-deficient and wild-type littermates, gray and white matter volumes were decreased as compared with uninjured mice. Numbers of motoneurons and parvalbumin-expressing interneurons were also reduced in both genotypes. Numbers of interneurons in injured mutant mice were lower than in wild-type littermates. Whereas injury did not affect numbers of astrocytes and oligodendrocytes in the gray matter, numbers of microglia\/macrophages were increased. In the mutant white matter, numbers of oligodendrocytes were reduced, with no changes in numbers of astrocytes and microglia. A loss of motoneurons and interneurons was observed in both genotypes, but loss of interneurons was more prominent in the absence of CHL1. We propose that, after injury, CHL1 deficiency causes deficits in structural outcome not seen after injury of wild-type mice.<\/jats:p>","DOI":"10.3390\/biom15091247","type":"journal-article","created":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T08:01:01Z","timestamp":1756454461000},"page":"1247","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Consequences of Adhesion Molecule Close Homolog of L1 Deficiency for Neurons and Glial Cells in the Mouse Spinal Cord After Injury"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0132-4985","authenticated-orcid":false,"given":"Igor","family":"Jakovcevski","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Anatomie und Klinische Morphologie, Universit\u00e4t Witten\/Herdecke, 58455 Witten, Germany"},{"name":"Department of Neuroanatomy and Molecular Brain Research, Institute of Anatomy, Ruhr-Universit\u00e4t Bochum, 44780 Bochum, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayse","family":"Acar","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Anatomie und Klinische Morphologie, Universit\u00e4t Witten\/Herdecke, 58455 Witten, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin","family":"Schwindenhammer","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Anatomie und Klinische Morphologie, Universit\u00e4t Witten\/Herdecke, 58455 Witten, Germany"},{"name":"Department of Neuroanatomy and Molecular Brain Research, Institute of Anatomy, Ruhr-Universit\u00e4t Bochum, 44780 Bochum, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7413-0305","authenticated-orcid":false,"given":"Mohammad I. K.","family":"Hamad","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain P.O. Box 15551, United Arab Emirates"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gebhard","family":"Reiss","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Anatomie und Klinische Morphologie, Universit\u00e4t Witten\/Herdecke, 58455 Witten, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2478-5610","authenticated-orcid":false,"given":"Eckart","family":"F\u00f6rster","sequence":"additional","affiliation":[{"name":"Department of Neuroanatomy and Molecular Brain Research, Institute of Anatomy, Ruhr-Universit\u00e4t Bochum, 44780 Bochum, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melitta","family":"Schachner","sequence":"additional","affiliation":[{"name":"W. M. Keck Center for Collaborative Neuroscience, Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ 08854, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e11505","DOI":"10.15252\/emmm.201911505","article-title":"Therapeutic repair for spinal cord injury: Combinatory approaches to address a multifaceted problem","volume":"12","author":"Griffin","year":"2020","journal-title":"EMBO Mol. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1152\/physrev.00017.2017","article-title":"The biology of regeneration failure and Success after spinal cord injury","volume":"98","author":"Tran","year":"2018","journal-title":"Physiol. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.semcdb.2022.07.001","article-title":"Moving through the crowd. 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