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Landesbibliothek Jena","award":["512648189"],"award-info":[{"award-number":["512648189"]}]},{"name":"Open Access Publication Fund of the Thueringer Universitaets\u2014und Landesbibliothek Jena","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior, Brasil (CAPES)","award":["2021\/14096-6"],"award-info":[{"award-number":["2021\/14096-6"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior, Brasil (CAPES)","award":["2023\/15593-9"],"award-info":[{"award-number":["2023\/15593-9"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior, Brasil (CAPES)","award":["2023\/00955\u20132"],"award-info":[{"award-number":["2023\/00955\u20132"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior, Brasil (CAPES)","award":["302928\/2021\u20139"],"award-info":[{"award-number":["302928\/2021\u20139"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior, Brasil (CAPES)","award":["512648189"],"award-info":[{"award-number":["512648189"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior, Brasil (CAPES)","award":["001"],"award-info":[{"award-number":["001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Bats are great models for studying repetitive DNAs due to their compact genomes and extensive chromosomal rearrangements. Here, we investigated the repetitive DNA content of two phyllostomid bat species, Artibeus lituratus (2nn = 30\u2640\/31\u2642) and Carollia perspicillata (2n = 20\u2640\/21\u2642), both harboring a multiple XY1Y2 sex chromosome system. Satellite DNA (satDNA) libraries were isolated and characterized, revealing four and ten satDNA families in A. lituratus and C. perspicillata, respectively. These sequences, along with selected microsatellites, were in situ mapped onto chromosomes in both species and phylogenetically related taxa. SatDNAs showed strong accumulation in centromeric and subtelomeric regions, especially pericentromeric areas. Cross-species mapping with C. perspicillata-derived probes indicated terminal localization patterns in other bat species, suggesting conserved distribution. Microsatellites co-localized with 45S rDNA clusters on the neo-sex chromosomes. Additionally, genomic hybridization revealed a male-specific signal on the Y1 chromosome, pointing to potential sex-linked repetitive regions. These findings confirm that bat genomes display relatively low amounts of repetitive DNA compared to other mammals and underscore the role of these elements in genome organization and sex chromosome evolution in phyllostomid bats.<\/jats:p>","DOI":"10.3390\/biom15091248","type":"journal-article","created":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T08:01:01Z","timestamp":1756454461000},"page":"1248","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Repetitive DNAs and Karyotype Evolution in Phyllostomid Bats (Chiroptera: Phyllostomidae)"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3374-2233","authenticated-orcid":false,"given":"Geize Aparecida","family":"Deon","sequence":"first","affiliation":[{"name":"Departamento de Gen\u00e9tica e Evolu\u00e7\u00e3o, Universidade Federal de S\u00e3o Carlos, S\u00e3o Carlos 13565-905, S\u00e3o Paulo, Brazil"},{"name":"Institute of Applied Ecology, Faculty of Science and Technology, University of Canberra, Australian Capital Territory, Canberra 2617, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4763-1347","authenticated-orcid":false,"given":"Tariq","family":"Ezaz","sequence":"additional","affiliation":[{"name":"Institute of Applied Ecology, Faculty of Science and Technology, University of Canberra, Australian Capital Territory, Canberra 2617, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7919-0262","authenticated-orcid":false,"given":"Jos\u00e9 Henrique Forte","family":"Stornioli","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias, Universidade Estadual Paulista, Bauru 17033-360, S\u00e3o Paulo, Brazil"},{"name":"Department of Genetics, Institute of Biological Sciences and Health, Rural Federal University of Rio de Janeiro, Serop\u00e9dica 23897-000, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Zeni","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias, Universidade Estadual Paulista, Bauru 17033-360, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3887-3283","authenticated-orcid":false,"given":"Anderson Jos\u00e9 Baia","family":"Gomes","sequence":"additional","affiliation":[{"name":"Instituto Federal de Educa\u00e7\u00e3o, Ci\u00eancia e Tecnologia, Abaetetuba 68440-000, Par\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6773-3856","authenticated-orcid":false,"given":"Pr\u00edncia Grejo","family":"Setti","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica e Evolu\u00e7\u00e3o, Universidade Federal de S\u00e3o Carlos, S\u00e3o Carlos 13565-905, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6315-3352","authenticated-orcid":false,"given":"Edivaldo Herculano Correa","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Citogen\u00f4mica e Mutag\u00eanese Ambiental, Instituto Evandro Chagas, Ananindeua 67030-000, Par\u00e1, Brazil"},{"name":"Instituto de Ci\u00eancias Exatas e Naturais, Universidade Federal do Par\u00e1, Bel\u00e9m 66075-110, Par\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8845-3845","authenticated-orcid":false,"given":"F\u00e1bio","family":"Porto-Foresti","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias, Universidade Estadual Paulista, Bauru 17033-360, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Utsunomia","sequence":"additional","affiliation":[{"name":"Faculdade de 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Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1007\/s10577-015-9499-z","article-title":"Repetitive DNA in Eukaryotic Genomes","volume":"23","author":"Biscotti","year":"2015","journal-title":"Chromosome Res."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Pa\u00e7o, A., Freitas, R., and Vieira-da-Silva, A. 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