{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:31:10Z","timestamp":1790692270394,"version":"4.1.0"},"reference-count":53,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:00:00Z","timestamp":1758672000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82301476"],"award-info":[{"award-number":["82301476"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2024A03J1058"],"award-info":[{"award-number":["2024A03J1058"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2025A04J4044"],"award-info":[{"award-number":["2025A04J4044"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Science and Technology Projects in Guangzhou","award":["82301476"],"award-info":[{"award-number":["82301476"]}]},{"name":"the Science and Technology Projects in Guangzhou","award":["2024A03J1058"],"award-info":[{"award-number":["2024A03J1058"]}]},{"name":"the Science and Technology Projects in Guangzhou","award":["2025A04J4044"],"award-info":[{"award-number":["2025A04J4044"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Cerebral ischemia\/reperfusion (I\/R) injury is a devastating neurological disorder with limited treatment options. Emerging evidence suggests that the N6-methyladenosine (m6A) modification and its regulatory factors play pivotal roles in the pathophysiology of I\/R. This study aimed to elucidate the function of METTL3-mediated m6A modification of the long non-coding RNA (lncRNA) AU020206 in ferroptosis during cerebral I\/R injury and to identify potential molecular targets for neuroprotection. A murine model of middle cerebral artery occlusion\/reperfusion (MCAO\/R) and N2a cells subjected to oxygen\u2013glucose deprivation\/reoxygenation (OGD\/R) were established to assess m6A levels and ferroptosis-related changes. Effects of METTL3 overexpression and lncRNA-AU020206 silencing on neuronal apoptosis, inflammation, and ferroptosis were investigated in vitro and in vivo. The interaction between lncRNA-AU020206 and YTHDC2 and the resulting regulation of SLC7A11 mRNA stability and GPX4 expression were evaluated using molecular and biochemical assays. Both MCAO\/R mice and OGD\/R-treated N2a cells exhibited decreased m6A levels and upregulation of lncRNA-AU020206 accompanied by enhanced ferroptosis. METTL3 overexpression increased the m6A modification of AU020206, promoting its degradation and attenuating neuronal injury, whereas silencing AU020206 or overexpressing YTHDC2 decreased SLC7A11 mRNA stability and enhanced ferroptosis. Restoring the expression of SLC7A11\/GPX4 can enhance cell viability, alleviate neuronal apoptosis, and reduce Fe2+ overload. Disruption of the METTL3\u2013AU020206\u2013YTHDC2 axis abolished these neuroprotective effects. METTL3-mediated m6A modification of lncRNA-AU020206 restrained ferroptosis and neuronal injury in cerebral I\/R by maintaining the stability of the SLC7A11\/GPX4 axis via interactions with YTHDC2. Targeting this epitranscriptomic signalling pathway may represent a promising therapeutic strategy for the treatment of ischemic stroke and related neurological disorders.<\/jats:p>","DOI":"10.3390\/biom15101353","type":"journal-article","created":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T08:19:45Z","timestamp":1758701985000},"page":"1353","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["METTL3-Driven m6A Epigenetic Remodeling of lncRNA-AU020206 Stabilizes SLC7A11 via YTHDC2 Attenuates Apoptosis and Ferroptosis in Cerebral Ischemia\/Reperfusion Injury"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3798-2979","authenticated-orcid":false,"given":"Hao","family":"Zhang","sequence":"first","affiliation":[{"name":"Institute of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China"},{"name":"Guangzhou Huateng Bioscience Corporation, Guangzhou 510700, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajin","family":"Guan","sequence":"additional","affiliation":[{"name":"Guangzhou Huateng Bioscience Corporation, Guangzhou 510700, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yilin","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0651-661X","authenticated-orcid":false,"given":"Xiaoou","family":"Sun","sequence":"additional","affiliation":[{"name":"Institute of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1016\/j.amjmed.2021.07.027","article-title":"Ischemic stroke","volume":"134","author":"Feske","year":"2021","journal-title":"Am. J. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1001\/jama.2020.26867","article-title":"Diagnosis and management of transient ischemic attack and acute ischemic stroke: A review","volume":"325","author":"Mendelson","year":"2021","journal-title":"JAMA"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1161\/STROKEAHA.120.031786","article-title":"Acute ischemic stroke and COVID-19: An analysis of 27 676 patients","volume":"52","author":"Qureshi","year":"2021","journal-title":"Stroke"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1056\/NEJMoa2214403","article-title":"Trial of endovascular thrombectomy for large ischemic strokes","volume":"388","author":"Sarraj","year":"2023","journal-title":"N. Engl. J. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1212\/CON.0000000000000840","article-title":"Update on treatment of acute ischemic stroke","volume":"26","author":"Rabinstein","year":"2020","journal-title":"Contin. Lifelong Learn. Neurol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Jurcau, A., and Simion, A. (2021). Neuroinflammation in cerebral ischemia and ischemia\/reperfusion injuries: From pathophysiology to therapeutic strategies. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23010014"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1016\/j.molcel.2023.01.006","article-title":"RNA m6A methylation across the transcriptome","volume":"83","author":"Sendinc","year":"2023","journal-title":"Mol. Cell"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4209","DOI":"10.1016\/j.molcel.2021.07.038","article-title":"Interaction of tau with HNRNPA2B1 and N(6)-methyladenosine RNA mediates the progression of tauopathy","volume":"81","author":"Jiang","year":"2021","journal-title":"Mol. Cell"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2040622320916024","DOI":"10.1177\/2040622320916024","article-title":"N(6)-methyladenosine demethylases Alkbh5\/Fto regulate cerebral ischemia-reperfusion injury","volume":"11","author":"Xu","year":"2020","journal-title":"Ther. Adv. Chronic Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2838","DOI":"10.1182\/blood.2021011707","article-title":"A critical role of nuclear m6A reader YTHDC1 in leukemogenesis by regulating MCM complex-mediated DNA replication","volume":"138","author":"Sheng","year":"2021","journal-title":"Blood"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1038\/s41418-024-01329-y","article-title":"The m(6)A methyltransferase METTL3 drives neuroinflammation and neurotoxicity through stabilizing BATF mRNA in microglia","volume":"32","author":"Wu","year":"2025","journal-title":"Cell Death Differ."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Bridges, M.C., Daulagala, A.C., and Kourtidis, A. (2021). LNCcation: lncRNA localization and function. J. Cell Biol., 220.","DOI":"10.1083\/jcb.202009045"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1038\/s41419-020-03021-8","article-title":"Oxygen glucose deprivation\/re-oxygenation-induced neuronal cell death is associated with Lnc-D63785 m6A methylation and miR-422a accumulation","volume":"11","author":"Xu","year":"2020","journal-title":"Cell Death Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"216420","DOI":"10.1016\/j.canlet.2023.216420","article-title":"LIMK1 m(6)A-RNA methylation recognized by YTHDC2 induces 5-FU chemoresistance in colorectal cancer via endoplasmic reticulum stress and stress granule formation","volume":"576","author":"Chen","year":"2023","journal-title":"Cancer Lett."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1101\/gad.349190.121","article-title":"YTHDC2 control of gametogenesis requires helicase activity but not m(6)A binding","volume":"36","author":"Saito","year":"2022","journal-title":"Genes Dev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1038\/s41580-020-00324-8","article-title":"Ferroptosis: Mechanisms, biology and role in disease","volume":"22","author":"Jiang","year":"2021","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2215","DOI":"10.1016\/j.molcel.2022.03.022","article-title":"Ferroptosis at the intersection of lipid metabolism and cellular signaling","volume":"82","author":"Liang","year":"2022","journal-title":"Mol. Cell"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Yuan, Y., Zhai, Y., Chen, J., Xu, X., and Wang, H. (2021). Kaempferol Ameliorates Oxygen-Glucose Deprivation\/Reoxygenation-Induced Neuronal Ferroptosis by Activating Nrf2\/SLC7A11\/GPX4 Axis. Biomolecules, 11.","DOI":"10.3390\/biom11070923"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1038\/s41418-019-0380-z","article-title":"ATF3 promotes erastin-induced ferroptosis by suppressing system Xc\u2212","volume":"27","author":"Wang","year":"2020","journal-title":"Cell Death Differ."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3918393","DOI":"10.1155\/2023\/3918393","article-title":"The Effect of N6-Methyladenosine Regulators and m6A Reader YTHDC1-Mediated N6-Methyladenosine Modification Is Involved in Oxidative Stress in Human Aortic Dissection","volume":"2023","author":"Yin","year":"2023","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/j.brainresbull.2018.05.015","article-title":"Neuroprotective Effects of Isosteviol Sodium through Increasing CYLD by the Downregulation miRNA-181b","volume":"140","author":"Zhang","year":"2018","journal-title":"Brain Res. Bull."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2603","DOI":"10.1016\/j.jstrokecerebrovasdis.2017.06.023","article-title":"Isosteviol Sodium Protects Against Permanent Cerebral Ischemia Injury in Mice via Inhibition of NF-\u03baB\u2013Mediated Inflammatory and Apoptotic Responses","volume":"26","author":"Zhang","year":"2017","journal-title":"J. Stroke Cerebrovasc. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1248\/bpb.b17-00921","article-title":"Isosteviol sodium inhibits astrogliosis after cerebral ischemia\/reperfusion injury in rats","volume":"41","author":"Zhang","year":"2018","journal-title":"Biol. Pharm. Bull."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1111\/cns.12589","article-title":"Lentivirus-Mediated Overexpression of MicroRNA-210 Improves Long-Term Outcomes after Focal Cerebral Ischemia in Mice","volume":"22","author":"Zeng","year":"2016","journal-title":"CNS Neurosci. Ther."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1161\/01.STR.32.4.1005","article-title":"Therapeutic benefit of intravenous administration of bone marrow stromal cells after cerebral ischemia in rats","volume":"32","author":"Chen","year":"2001","journal-title":"Stroke"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1379162","DOI":"10.1155\/2016\/1379162","article-title":"Neuroprotective Effects of Isosteviol Sodium Injection on Acute Focal Cerebral Ischemia in Rats","volume":"2016","author":"Hui","year":"2016","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"153849","DOI":"10.1016\/j.phymed.2021.153849","article-title":"Protocatechuic aldehyde prevents ischemic injury by attenuating brain microvascular endothelial cell pyroptosis via lncRNA Xist","volume":"94","author":"Guo","year":"2022","journal-title":"Phytomedicine"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.4155\/fmc-2023-0125","article-title":"Exploring ACSL4\/LPCAT3\/ALOX15 and SLC7A11\/GPX4\/NFE2L2 as potential targets in ferroptosis-based cancer therapy","volume":"15","author":"Gupta","year":"2023","journal-title":"Futur. Med. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e00921","DOI":"10.1002\/brb3.921","article-title":"Curcumin protects neural cells against ischemic injury in N2a cells and mouse brain with ischemic stroke","volume":"8","author":"Xie","year":"2018","journal-title":"Brain Behav."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"142","DOI":"10.14715\/cmb\/2024.70.3.21","article-title":"Knockdown of lncRNA AU020206 could inhibit microglia apoptosis in ischemic stroke","volume":"70","author":"Chen","year":"2024","journal-title":"Cell. Mol. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"621","DOI":"10.2174\/1574893614666190131161002","article-title":"PlncRNADB: A repository of plant lncRNAs and lncRNA-RBP protein interactions","volume":"14","author":"Bai","year":"2019","journal-title":"Curr. Bioinform."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1186\/s12967-024-05293-6","article-title":"The biological function of the N6-Methyladenosine reader YTHDC2 and its role in diseases","volume":"22","author":"Wu","year":"2024","journal-title":"J. Transl. Med."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Puvvula, P.K. (2019). LncRNAs Regulatory Networks in Cellular Senescence. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20112615"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"11515","DOI":"10.1007\/s11033-022-07819-x","article-title":"LncRNAs in neuropsychiatric disorders and computational insights for their prediction","volume":"49","author":"Baruah","year":"2022","journal-title":"Mol. Biol. Rep."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1550","DOI":"10.1016\/j.ymthe.2023.02.008","article-title":"Roles of lncRNAs in brain development and pathogenesis: Emerging therapeutic opportunities","volume":"31","author":"Srinivas","year":"2023","journal-title":"Mol. Ther."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Wang, L., Li, S., Stone, S.S., Liu, N., Gong, K., Ren, C., Sun, K., Zhang, C., and Shao, G. (2022). The Role of the lncRNA MALAT1 in Neuroprotection against Hypoxic\/Ischemic Injury. Biomolecules, 12.","DOI":"10.3390\/biom12010146"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"91","DOI":"10.3892\/ijmm.2022.5147","article-title":"An update on the functional roles of long non-coding RNAs in ischemic injury (Review)","volume":"50","author":"Cao","year":"2022","journal-title":"Int. J. Mol. Med."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1007\/978-981-13-1426-1_25","article-title":"The Role of Circular RNAs in Cerebral Ischemic Diseases: Ischemic Stroke and Cerebral Ischemia\/Reperfusion Injury","volume":"1087","author":"Yang","year":"2018","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1039\/D0MO00077A","article-title":"Role of the lncRNA-mRNA network in atherosclerosis using ox-low-density lipoprotein-induced macrophage-derived foam cells","volume":"16","author":"Zhang","year":"2020","journal-title":"Mol. Omics"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3224","DOI":"10.4081\/ejh.2021.3224","article-title":"Long noncoding RNA Meg3 mediates ferroptosis induced by oxygen and glucose deprivation combined with hyperglycemia in rat brain microvascular endothelial cells, through modulating the p53\/GPX4 axis","volume":"65","author":"Chen","year":"2021","journal-title":"Eur. J. Histochem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"118305","DOI":"10.1016\/j.lfs.2020.118305","article-title":"LncRNA PVT1 regulates ferroptosis through miR-214-mediated TFR1 and p53","volume":"260","author":"Lu","year":"2020","journal-title":"Life Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.gpb.2016.12.005","article-title":"Transcriptional and post-transcriptional gene regulation by long non-coding RNA","volume":"15","author":"Dykes","year":"2017","journal-title":"Genom. Proteom. Bioinform."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Wang, H., Yuan, J., Dang, X., Shi, Z., Ban, W., and Ma, D. (2021). Mettl14-mediated m6A modification modulates neuron apoptosis during the repair of spinal cord injury by regulating the transformation from pri-mir-375 to miR-375. Cell Biosci., 11.","DOI":"10.1186\/s13578-020-00526-9"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"123848","DOI":"10.1016\/j.envpol.2024.123848","article-title":"Increased m(6)A modification of BDNF mRNA via FTO promotes neuronal apoptosis following aluminum-induced oxidative stress","volume":"349","author":"Song","year":"2024","journal-title":"Environ. Pollut."},{"key":"ref_45","first-page":"1631","article-title":"METTL3 plays multiple functions in biological processes","volume":"10","author":"Liu","year":"2020","journal-title":"Am. J. Cancer Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e201800113","DOI":"10.26508\/lsa.201800113","article-title":"Dynamic m(6)A methylation facilitates mRNA triaging to stress granules","volume":"1","author":"Anders","year":"2018","journal-title":"Life Sci. Alliance"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1038\/cr.2017.99","article-title":"Ythdc2 is an N6-methyladenosine binding protein that regulates mammalian spermatogenesis","volume":"27","author":"Hsu","year":"2017","journal-title":"Cell Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1007\/s11010-022-04570-6","article-title":"METTL3-modified lncRNA-SNHG8 binds to PTBP1 to regulate ALAS2 expression to increase oxidative stress and promote myocardial infarction","volume":"478","author":"Tang","year":"2023","journal-title":"Mol. Cell. Biochem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/j.jhep.2023.03.016","article-title":"ATF4 suppresses hepatocarcinogenesis by inducing SLC7A11 (xCT) to block stress-related ferroptosis","volume":"79","author":"He","year":"2023","journal-title":"J. Hepatol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"115021","DOI":"10.1016\/j.jep.2022.115021","article-title":"Rehmannioside A improves cognitive impairment and alleviates ferroptosis via activating PI3K\/AKT\/Nrf2 and SLC7A11\/GPX4 signaling pathway after ischemia","volume":"289","author":"Fu","year":"2022","journal-title":"J. Ethnopharmacol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"114541","DOI":"10.1016\/j.expneurol.2023.114541","article-title":"Rhein attenuates cerebral ischemia-reperfusion injury via inhibition of ferroptosis through NRF2\/SLC7A11\/GPX4 pathway","volume":"369","author":"Liu","year":"2023","journal-title":"Exp. Neurol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Liu, H., Yue, Q., Zhang, W., Ding, Q., Yang, J., Lin, M., and Sun, J. (2024). Xinglou Chengqi Decoction Protects against Cerebral Ischemia\/Reperfusion Injury by Inhibiting Ferroptosis via SLC7A11\/GPX4 Signaling. Adv. Biol., 8.","DOI":"10.1002\/adbi.202400180"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"8693664","DOI":"10.1155\/2022\/8693664","article-title":"Treadmill Training Reduces Cerebral Ischemia-Reperfusion Injury by Inhibiting Ferroptosis through Activation of SLC7A11\/GPX4","volume":"2022","author":"Liu","year":"2022","journal-title":"Oxidative Med. Cell. Longev."}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/15\/10\/1353\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:48:37Z","timestamp":1760035717000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/15\/10\/1353"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,24]]},"references-count":53,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,10]]}},"alternative-id":["biom15101353"],"URL":"https:\/\/doi.org\/10.3390\/biom15101353","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,24]]}}}