{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T20:35:21Z","timestamp":1783456521119,"version":"3.55.0"},"reference-count":80,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005416","name":"Research Council of Norway","doi-asserted-by":"publisher","award":["328654"],"award-info":[{"award-number":["328654"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Marine diatoms are prolific producers of bioactive metabolites, but Arctic species remain underexplored as sources of antibacterial and antibiofilm agents. Here, seven species were grown in photobioreactors (PBRs) and systematically screened for antibacterial, antibiofilm, and cytotoxic activities. All strains inhibited Gram-positive bacteria, and four reduced Staphylococcus epidermidis biofilm formation. Porosira glacialis emerged as a lead species, combining potent antibiofilm activity with favourable traits for large-scale cultivation, and no detectable cytotoxicity. Bioactivity-guided fractionation of P. glacialis yielded two antibiofilm compounds: methyl 3-hydroxyoctadecanoate, the first time reported in diatoms and newly associated with antibiofilm bioactivity, and pheophorbide a, a chlorophyll degradation product. Both inhibited S. epidermidis biofilm formation without any observed cytotoxicity. Notably, Cylindrotheca closterium exhibited cultivation-dependent antibiofilm activity, underscoring the importance of growth conditions for metabolite production. These findings highlight the potential of Arctic diatoms as a sustainable source of antibiofilm agents and support further exploration of their metabolites for antimicrobial and industrial applications.<\/jats:p>","DOI":"10.3390\/biom15101482","type":"journal-article","created":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:17:35Z","timestamp":1761056255000},"page":"1482","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Arctic Diatoms as a Source of Antibiofilm Compounds: Identification of Methyl 3-Hydroxyoctadecanoate and Pheophorbide a"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1946-0600","authenticated-orcid":false,"given":"Marit","family":"Huizer","sequence":"first","affiliation":[{"name":"Natural Products and Medicinal Chemistry Research Group, Department of Pharmacy, UiT\u2014The Arctic University of Norway, NO-9037 Troms\u00f8, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renate","family":"Osvik","sequence":"additional","affiliation":[{"name":"Marbio, Norwegian College of Fishery Science, UiT\u2014The Arctic University of Norway, NO-9037 Troms\u00f8, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0354-986X","authenticated-orcid":false,"given":"Espen H.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Marbio, Norwegian College of Fishery Science, UiT\u2014The Arctic University of Norway, NO-9037 Troms\u00f8, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terje","family":"Vasskog","sequence":"additional","affiliation":[{"name":"Natural Products and Medicinal Chemistry Research Group, Department of Pharmacy, UiT\u2014The Arctic University of Norway, NO-9037 Troms\u00f8, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6059-060X","authenticated-orcid":false,"given":"Jeanette H.","family":"Andersen","sequence":"additional","affiliation":[{"name":"Marbio, Norwegian College of Fishery Science, UiT\u2014The Arctic University of Norway, NO-9037 Troms\u00f8, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0140-2066","authenticated-orcid":false,"given":"Kim","family":"van Wezel","sequence":"additional","affiliation":[{"name":"Marbio, Norwegian College of Fishery Science, UiT\u2014The Arctic University of Norway, NO-9037 Troms\u00f8, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans Christian","family":"Eilertsen","sequence":"additional","affiliation":[{"name":"Microalgae and Microbiomes, Norwegian College of Fishery Science, UiT\u2014The Arctic University of Norway, NO-9037 Troms\u00f8, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6287-7594","authenticated-orcid":false,"given":"Johan","family":"Isaksson","sequence":"additional","affiliation":[{"name":"Natural Products and Medicinal Chemistry Research Group, Department of Pharmacy, UiT\u2014The Arctic University of Norway, NO-9037 Troms\u00f8, Norway"},{"name":"Chemical Synthesis and Analysis Group, Department of Chemistry, UiT\u2014The Arctic University of Norway, NO-9037 Troms\u00f8, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9023-1958","authenticated-orcid":false,"given":"Kine \u00d8.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Natural Products and Medicinal Chemistry Research Group, Department of Pharmacy, UiT\u2014The Arctic University of Norway, NO-9037 Troms\u00f8, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2871-8888","authenticated-orcid":false,"given":"Richard A.","family":"Ingebrigtsen","sequence":"additional","affiliation":[{"name":"Microalgae and Microbiomes, Norwegian College of Fishery Science, UiT\u2014The Arctic University of Norway, NO-9037 Troms\u00f8, Norway"},{"name":"Cawthron Institute, Private Bag 2, Nelson 7042, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.lfs.2013.08.002","article-title":"Health applications of bioactive compounds from marine microalgae","volume":"93","year":"2013","journal-title":"Life Sci."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Hachicha, R., Elleuch, F., Hlima, H.B., Dubessay, P., de Baynast, H., Delattre, C., Pierre, G., Hachicha, R., Abdelkafi, S., and Michaud, P. (2022). Biomolecules from Microalgae and Cyanobacteria: Applications and Market Survey. Appl. Sci., 12.","DOI":"10.3390\/app12041924"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Patel, A.K., Albarico, F.P.J.B., Perumal, P.K., Vadrale, A.P., Ntan, C.T., Chau, H.T.B., Anwar, C., Wani, H.M.U.D., Pal, A., and Saini, R. (2022). Algae as an emerging source of bioactive pigments. Bioresour. Technol., 351.","DOI":"10.1016\/j.biortech.2022.126910"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Pereira, A.G., Otero, P., Echave, J., Carreira-Casais, A., Chamorro, F., Collazo, N., Jaboui, A., Louren\u00e7o-Lopes, C., Simal-Gandara, J., and Prieto, M.A. (2021). Xanthophylls from the Sea: Algae as Source of Bioactive Carotenoids. Mar. Drugs, 19.","DOI":"10.3390\/md19040188"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/j.rser.2018.04.034","article-title":"Exploring the potential of microalgae for new biotechnology applications and beyond: A review","volume":"92","author":"Rizwan","year":"2018","journal-title":"Renew. Sustain. Energy Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1263\/jbb.101.87","article-title":"Commercial applications of microalgae","volume":"101","author":"Spolaore","year":"2006","journal-title":"J. Biosci. Bioeng."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1038\/nature08057","article-title":"The life of diatoms in the world\u2019s oceans","volume":"459","author":"Armbrust","year":"2009","journal-title":"Nature"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1126\/science.281.5374.237","article-title":"Primary Production of the Biosphere: Integrating Terrestrial and Oceanic Components","volume":"281","author":"Field","year":"1998","journal-title":"Science"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"137960","DOI":"10.1016\/j.scitotenv.2020.137960","article-title":"Wealth from waste: Diatoms as tools for phycoremediation of wastewater and for obtaining value from the biomass","volume":"724","author":"Marella","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1029\/95GB01070","article-title":"Production and dissolution of biogenic silica in the ocean: Revised global estimates, comparison with regional data and relationship to biogenic sedimentation","volume":"9","author":"Nelson","year":"1995","journal-title":"Global Biogeochem. Cycles"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.biotechadv.2011.05.016","article-title":"Algal chemodiversity and bioactivity: Sources of natural variability and implications for commercial application","volume":"29","author":"Stengel","year":"2011","journal-title":"Biotechnol. Adv."},{"key":"ref_12","unstructured":"Aliva, C. (2021). Chemical war in marine animal forests: Natural products and chemical interactions. Perspectives on the Marine Animal Forests of the World, Springer."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4232","DOI":"10.3390\/md11114232","article-title":"Chemical Diversity as a Function of Temperature in Six Northern Diatom Species","volume":"11","author":"Huseby","year":"2013","journal-title":"Mar. Drugs"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1007\/s10811-008-9367-8","article-title":"Metabolite profiling of the benthic diatom Cocconeis scutellum by GC-MS","volume":"21","author":"Nappo","year":"2008","journal-title":"J. Appl. Phycol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1007\/s10811-015-0631-4","article-title":"Light and temperature effects on bioactivity in diatoms","volume":"28","author":"Ingebrigtsen","year":"2016","journal-title":"J. Appl. Phycol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Lauritano, C., Andersen, J.H., Hansen, E., Albrigtsen, M., Escalera, L., Esposito, F., Helland, K., Hanssen, K.\u00d8., Romano, G., and Ianora, A. (2016). Bioactivity Screening of Microalgae for Antioxidant, Anti-Inflammatory, Anticancer, Anti-Diabetes, and Antibacterial Activities. Front. Mar. Sci., 3.","DOI":"10.3389\/fmars.2016.00068"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Svenning, J.B., Vasskog, T., Campbell, K., B\u00e6verud, A.H., Myhre, T.N., Dalheim, L., Forgereau, Z.L., Osanen, J.E., Hansen, E.H., and Bernstein, H.C. (2024). Lipidome Plasticity Enables Unusual Photosynthetic Flexibility in Arctic vs. Temperate Diatoms. Mar. Drugs, 22.","DOI":"10.3390\/md22020067"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1146\/annurev.ecolsys.39.110707.173549","article-title":"Trait-Based Community Ecology of Phytoplankton","volume":"39","author":"Litchman","year":"2008","journal-title":"Annu. Rev. Ecol. Evol. Syst."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/j.algal.2017.07.030","article-title":"Bioprospecting North Atlantic microalgae with fast growth and high polyunsaturated fatty acid (PUFA) content for microalgae-based technologies","volume":"26","author":"Erga","year":"2017","journal-title":"Algal Res."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Steinr\u00fccken, P., Mj\u00f8s, S.A., Prestegard, S.K., and Erga, S.R. (2018). Enhancing EPA content in an Arctic diatom: A factorial design study to evaluate interactive effects of growth factors. Front. Plant Sci., 9.","DOI":"10.3389\/fpls.2018.00491"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1111\/j.2041-1014.2009.00553.x","article-title":"A novel bioactivity of omega-3 polyunsaturated fatty acids and their ester derivatives","volume":"25","author":"Huang","year":"2010","journal-title":"Mol. Oral Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3627","DOI":"10.1016\/j.procbio.2005.02.020","article-title":"Omega-3\/6 fatty acids: Alternative sources of production","volume":"40","author":"Ward","year":"2005","journal-title":"Process Biochem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3831","DOI":"10.1038\/ncomms4831","article-title":"Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology","volume":"5","author":"Daboussi","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Marella, T.K., and Tiwari, A. (2023). Cultivation of diatoms in photobioreactors. Current Developments in Biotechnology and Bioengineering, Elsevier.","DOI":"10.1016\/B978-0-323-99911-3.00009-9"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1016\/j.rser.2015.12.162","article-title":"Concepts and studies on lipid and pigments of microalgae: A review","volume":"58","author":"Filho","year":"2016","journal-title":"Renew. Sustain. Energy Rev."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1089\/ind.2020.0031","article-title":"Bioactivity of a Marine Diatom (Porosira glacialis [Grunow] J\u00f6rgensen 1905) Cultivated with and without Factory Smoke CO2","volume":"17","author":"Osvik","year":"2021","journal-title":"Ind. Biotechnol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Osvik, R.D., Ingebrigtsen, R.A., Norrbin, M.F., Andersen, J.H., Eilertsen, H.C., and Hansen, E.H. (2021). Adding Zooplankton to the OSMAC Toolkit: Effect of Grazing Stress on the Metabolic Profile and Bioactivity of a Diatom. Mar. Drugs, 19.","DOI":"10.3390\/md19020087"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Trentin, R., Moschin, E., Cust\u00f3dio, L., and Moro, I. (2024). Bioprospection of the Antarctic Diatoms Craspedostauros ineffabilis IMA082A and Craspedostauros zucchelli IMA088A. Mar. Drugs, 22.","DOI":"10.3390\/md22010035"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.nbt.2022.12.001","article-title":"Exploring Nordic microalgae as a potential novel source of antioxidant and bioactive compounds","volume":"73","author":"Vigara","year":"2023","journal-title":"N. Biotechnol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Lauritano, C., Mart\u00edn, J., De La Cruz, M., Reyes, F., Romano, G., and Ianora, A. (2018). First identification of marine diatoms with anti-tuberculosis activity. Sci. Rep., 8.","DOI":"10.1038\/s41598-018-20611-x"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Lauritano, C., Helland, K., Riccio, G., Andersen, J.H., Ianora, A., and Hansen, E.H. (2020). Lysophosphatidylcholines and Chlorophyll-Derived Molecules from the Diatom Cylindrotheca closterium with Anti-Inflammatory Activity. Mar. Drugs, 18.","DOI":"10.3390\/md18030166"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"102475","DOI":"10.1016\/j.algal.2021.102475","article-title":"Bioprospecting of fucoxanthin from diatoms\u2014Challenges and perspectives","volume":"60","author":"Seth","year":"2021","journal-title":"Algal Res."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Bhattacharjya, R., Marella, T.K., Tiwari, A., Saxena, A., Singh, P.K., and Mishra, B. (2020). Bioprospecting of marine diatoms Thalassiosira, Skeletonema and Chaetoceros for lipids and other value-added products. Bioresour. Technol.","DOI":"10.1016\/j.biortech.2020.124073"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Guieysse, B., and Plouviez, M. (2024). Microalgae cultivation: Closing the yield gap from laboratory to field scale. Front. Bioeng. Biotechnol., 12.","DOI":"10.3389\/fbioe.2024.1359755"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Eilertsen, H.C., Eriksen, G.K., Bergum, J.S., Str\u00f8mholt, J., Elvevoll, E., Eilertsen, K.E., Heimstad, E.S., Gi\u00e6ver, I.H., Israelsen, L., and Svenning, J.B. (2022). Mass Cultivation of Microalgae: I. Experiences with Vertical Column Airlift Photobioreactors, Diatoms and CO2 Sequestration. Appl. Sci., 12.","DOI":"10.3390\/app12063082"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Eilertsen, H.C., Str\u00f8mholt, J., Bergum, J.S., Eriksen, G.K., and Ingebrigtsen, R. (2023). Mass Cultivation of Microalgae: II. A Large Species Pulsing Blue Light Concept. BioTech, 12.","DOI":"10.3390\/biotech12020040"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1080\/09670262.2020.1758347","article-title":"Physiological and morphological response of marine diatom Cylindrotheca closterium (Bacillariophyceae) exposed to Cadmium","volume":"56","year":"2021","journal-title":"Eur. J. Phycol."},{"key":"ref_38","unstructured":"Vihtakari, M. (2025, October 15). ggOceanMaps: Plot Data on Oceanographic Maps Using \u2018ggplot2\u2019. [R Package Version 2.2.0]. Available online: https:\/\/mikkovihtakari.github.io\/ggOceanMaps."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1093\/plankt\/fbs004","article-title":"Functional diversity in cryptic species of Chaetoceros socialis Lauder (Bacillariophyceae)","volume":"34","author":"Degerlund","year":"2012","journal-title":"J. Plankton Res."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Eilertsen, H.C., Elvevoll, E., Gi\u00e6ver, I.H., Svenning, J.B., Dalheim, L., Svalheim, R.A., Vang, B., Siikavuopio, S., Drag\u00f8y, R., and Ingebrigtsen, R.A. (2021). Inclusion of photoautotrophic cultivated diatom biomass in salmon feed can deter lice. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0255370"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2412","DOI":"10.1073\/pnas.1108062109","article-title":"Daily bursts of biogenic cyanogen bromide (BrCN) control biofilm formation around a marine benthic diatom","volume":"109","author":"Vanelslander","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Mougin, J., Pavaux, A.S., Fanesi, A., Lopez, J., Pruvost, E., Guih\u00e9neuf, F., Sciandra, A., Briandet, R., and Lopes, F. (2024). Bacterial adhesion inhibition by microalgal EPSs from Cylindrotheca closterium and Tetraselmis suecica biofilms. Appl. Microbiol. Biotechnol., 108.","DOI":"10.1007\/s00253-023-12960-z"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Le Norcy, T., Niemann, H., Proksch, P., Linossier, I., Vall\u00e9e-R\u00e9hel, K., Hellio, C., and Fa\u00ff, F. (2017). Anti-Biofilm Effect of Biodegradable Coatings Based on Hemibastadin Derivative in Marine Environment. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18071520"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/S0147-6513(02)00077-5","article-title":"Sediment toxicity tests using benthic marine microalgae Cylindrotheca closterium (Ehremberg) Lewin and Reimann (Bacillariophyceae)","volume":"54","author":"Hampel","year":"2003","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3809","DOI":"10.1007\/s10811-020-02238-6","article-title":"Salinity tolerance in biogeographically different strains of the marine benthic diatom Cylindrotheca closterium (Bacillariophyceae)","volume":"32","author":"Glaser","year":"2020","journal-title":"J. Appl. Phycol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.protis.2006.10.001","article-title":"Reproductive Isolation among Sympatric Cryptic Species in Marine Diatoms","volume":"158","author":"Amato","year":"2007","journal-title":"Protist"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1080\/09670262.2012.741714","article-title":"Metabolic fingerprinting reveals differences between northern and southern strains of the cryptic diatom Chaetoceros socialis","volume":"47","author":"Huseby","year":"2012","journal-title":"Eur. J. Phycol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1080\/10412905.2009.9700209","article-title":"Volatiles of Minuartia recurva (all.) schinz et thell. subsp. recurva (caryophyllaceae) from Serbia","volume":"21","year":"2009","journal-title":"J. Essent. Oil Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"182","DOI":"10.25135\/rnp.94.18.07.324","article-title":"Triterpenoids from Acokanthera schimperi in Ethiopia","volume":"13","author":"Matebie","year":"2019","journal-title":"Rec. Nat. Prod."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/S0021-9258(19)45771-2","article-title":"The Structure of an Ornithine-containing Lipid from Thiobacillus thiooxidans","volume":"247","author":"Knoche","year":"1972","journal-title":"J. Biol. Chem."},{"key":"ref_51","first-page":"247","article-title":"Volatile Components of Geneps (Melicocca bijuga L.)","volume":"14","author":"Pino","year":"2002","journal-title":"Fruit"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1128\/br.18.2.147-163.1954","article-title":"Influence of trace amounts of fatty acids on the growth of microorganisms","volume":"18","author":"Nieman","year":"1954","journal-title":"Bacteriol. Rev."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Lang, I., Hodac, L., Friedl, T., and Feussner, I. (2011). Fatty acid profiles and their distribution patterns in microalgae: A comprehensive analysis of more than 2000 strains from the SAG culture collection. BMC Plant Biol., 11.","DOI":"10.1186\/1471-2229-11-124"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0031-9422(94)00913-E","article-title":"Fatty acid composition of 15 species of marine microalgae","volume":"39","author":"Zhukova","year":"1995","journal-title":"Phytochemistry"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Cora\u00e7a-Huber, D.C., Steixner, S., Wurm, A., and Nogler, M. (2021). Antibacterial and anti-biofilm activity of omega-3 polyunsaturated fatty acids against periprosthetic joint infections-isolated multi-drug resistant strains. Biomedicines, 9.","DOI":"10.3390\/biomedicines9040334"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.algal.2018.10.009","article-title":"Temperature dependent growth rate, lipid content and fatty acid composition of the marine cold-water diatom Porosira glacialis","volume":"37","author":"Svenning","year":"2019","journal-title":"Algal Res."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1629","DOI":"10.1007\/s00253-009-2355-3","article-title":"Antibacterial free fatty acids: Activities, mechanisms of action and biotechnological potential","volume":"85","author":"Desbois","year":"2009","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S0044-8486(98)00513-4","article-title":"Antibacterial activity of the marine diatom Skeletonema costatum against aquacultural pathogens","volume":"174","author":"Naviner","year":"1999","journal-title":"Aquaculture"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1139\/m83-056","article-title":"Production of antibacterial activities by two Bacillariophyceae grown in dialysis culture","volume":"29","author":"Cooper","year":"1983","journal-title":"Can. J. Microbiol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1139\/m66-120","article-title":"The antibacterial activity of marine planktonic algae","volume":"12","author":"Duff","year":"1966","journal-title":"Can. J. Microbiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1021\/np50035a010","article-title":"Antibacterial Constituents of the Diatom Navicula delognei","volume":"47","author":"Findlay","year":"1984","journal-title":"J. Nat. Prod."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1687","DOI":"10.2331\/suisan.58.1687","article-title":"Studies on the Autoinhibitor Produced by Skeletonema costatum-II. Isolation and Identification of an Autoinhibitor Produced by Skeletonema costatum","volume":"58","author":"Imada","year":"1992","journal-title":"Nippon Suisan Gakkaishi"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.jembe.2004.10.001","article-title":"Free fatty acids and sterols in the benthic spawn of aquatic molluscs, and their associated antimicrobial properties","volume":"316","author":"Benkendorff","year":"2005","journal-title":"J. Exp. Mar. Bio. Ecol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1007\/s10811-007-9257-5","article-title":"Algicidal activity of polyunsaturated fatty acids derived from Ulva fasciata and U. pertusa (Ulvaceae, Chlorophyta) on phytoplankton","volume":"20","author":"Alamsjah","year":"2007","journal-title":"J. Appl. Phycol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.aquatox.2006.09.011","article-title":"Cytotoxic effects of free fatty acids on phytoplankton algae and cyanobacteria","volume":"80","author":"Wu","year":"2006","journal-title":"Aquat. Toxicol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1128\/AAC.30.2.254","article-title":"Killing of Giardia lamblia by human milk is mediated by unsaturated fatty acids","volume":"30","author":"Rohrer","year":"1986","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1007\/s00705-005-0699-2","article-title":"Virucidal effect of lipids on visna virus, a lentivirus related to HIV","volume":"151","author":"Hilmarsson","year":"2006","journal-title":"Arch. Virol."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Cepas, V., Guti\u00e9rrez-Del-r\u00edo, I., L\u00f3pez, Y., Redondo-Blanco, S., Gabasa, Y., Iglesias, M.J., Soengas, R., Fern\u00e1ndez-Lorenzo, A., L\u00f3pez-Ib\u00e1\u00f1ez, S., and Villar, C.J. (2021). Microalgae and Cyanobacteria Strains as Producers of Lipids with Antibacterial and Antibiofilm Activity. Mar. Drugs, 19.","DOI":"10.3390\/md19120675"},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"L\u00f3pez, Y., and Soto, S.M. (2020). The Usefulness of Microalgae Compounds for of Microalgae Preventing Biofilm Infections Compounds for Preventing Biofilm Infections. Antibiotics, 9.","DOI":"10.3390\/antibiotics9010009"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1016\/j.tim.2020.03.014","article-title":"Fatty Acids as Antibiofilm and Antivirulence Agents","volume":"28","author":"Kumar","year":"2020","journal-title":"Trends Microbiol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/j.biotechadv.2007.02.001","article-title":"Biodiesel from microalgae","volume":"25","author":"Chisti","year":"2007","journal-title":"Biotechnol. Adv."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"120985","DOI":"10.1016\/j.fuel.2021.120985","article-title":"Scope for biodiesel and bioactive compounds production in the diatom Nitzschia punctata","volume":"300","author":"Saranya","year":"2021","journal-title":"Fuel"},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Sheehan, J., Dunahay, T., Benemann, J., and Roessler, P. (1998). Look Back at the U.S. Department of Energy\u2019s Aquatic Species Program: Biodiesel from Algae, National Renewable Energy Laboratory.","DOI":"10.2172\/15003040"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Vijay, K., Kiran, G.S., Divya, S., Thangavel, K., Thangavelu, S., Dhandapani, R., and Selvin, J. (2021). Fatty Acid Methyl Esters From the Coral-Associated Bacterium Pseudomonas aeruginosa Inhibit Virulence and Biofilm Phenotypes in Multidrug Resistant Staphylococcus aureus: An in vitro Approach. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.631853"},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Saide, A., Lauritano, C., and Ianora, A. (2020). Pheophorbide a: State of the Art. Mar. Drugs, 18.","DOI":"10.3390\/md18050257"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1111\/1348-0421.12133","article-title":"Antiviral activity of extracts from Morinda citrifolia leaves and chlorophyll catabolites, pheophorbide a and pyropheophorbide a, against hepatitis C virus","volume":"58","author":"Ratnoglik","year":"2014","journal-title":"Microbiol. Immunol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1016\/j.foodchem.2011.01.072","article-title":"Antioxidant properties of extract and fractions from Enteromorpha prolifera, a type of green seaweed","volume":"127","author":"Cho","year":"2011","journal-title":"Food Chem."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"5383","DOI":"10.1016\/j.bmc.2011.07.058","article-title":"Synthesis of pheophorbide-a conjugates with anticancer drugs as potential cancer diagnostic and therapeutic agents","volume":"19","author":"You","year":"2011","journal-title":"Bioorganic Med. Chem."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"103658","DOI":"10.1016\/j.micpath.2019.103658","article-title":"Lutein: A potential antibiofilm and antiquorum sensing molecule from green microalga Chlorella pyrenoidosa","volume":"135","author":"Sampathkumar","year":"2019","journal-title":"Microb. Pathog."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1080\/08927014.2019.1640214","article-title":"Biofouling in marine aquaculture: A review of recent research and developments","volume":"35","author":"Bannister","year":"2019","journal-title":"Biofouling"}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/15\/10\/1482\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:38:48Z","timestamp":1761057528000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/15\/10\/1482"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,21]]},"references-count":80,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,10]]}},"alternative-id":["biom15101482"],"URL":"https:\/\/doi.org\/10.3390\/biom15101482","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,21]]}}}