{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T22:33:41Z","timestamp":1785796421295,"version":"3.56.0"},"reference-count":58,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["AG 079189"],"award-info":[{"award-number":["AG 079189"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["AG 077700"],"award-info":[{"award-number":["AG 077700"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Parkinson\u2019s disease (PD) involves motor and cognitive impairment that nicotinic acetylcholine receptors (nAChRs) such as the \u03b17 subtype are responsible for regulating. The hippocampus, abundant in \u03b17 nAChRs, was quantitatively evaluated for [125I]\u03b1-bungarotoxin ([125I]\u03b1-Bgtx) binding to \u03b17 nAChRs in postmortem human PD (n = 26; 12 male, 14 female) and cognitively normal (CN) (n = 29; 14 male, 15 female) brain slices. Anti-ubiquitin and anti-\u03b1-synuclein immunostained adjacent slices were analyzed using QuPath. Autoradiographs of [125I]\u03b1-Bgtx radioligand binding were analyzed in OptiQuant. Ubiquitin and \u03b1-synuclein distribution generally aligned with the distribution of \u03b17 nAChRs detected by [125I]\u03b1-Bgtx. Binding of [125I]\u03b1-Bgtx in PD cases was significantly greater than CN with a 32% increase in gray matter binding. A weak positive correlation between age and [125I]\u03b1-Bgtx binding was found in both PD and CN. In comparison to Alzheimer\u2019s disease hippocampus, [125I]\u03b1-Bgtx binding in PD gray matter was higher by 41%. Differences in nAChR expression imply unique roles depending on the neurodegenerative pathology. PD may experience an increase in \u03b17 nAChRs as a compensatory mechanism to the loss in neurons, highlighting its neuroprotective capabilities. [125I]\u03b1-Bgtx shows potential as a radioligand for \u03b17 nAChRs to elucidate the complexities of PD pathology.<\/jats:p>","DOI":"10.3390\/biom15121686","type":"journal-article","created":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T15:31:17Z","timestamp":1764689477000},"page":"1686","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Assessment of [125I]a-Bungarotoxin Binding to a7 Nicotinic Acetylcholinergic Receptors in Hippocampus-Subiculum of Postmortem Human Parkinson\u2019s Disease Brain"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-3104-9523","authenticated-orcid":false,"given":"Fariha","family":"Karim","sequence":"first","affiliation":[{"name":"Preclinical Imaging, Department of Radiological Sciences, University of California-Irvine, Irvine, CA 92697, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6993-9131","authenticated-orcid":false,"given":"Allyson","family":"Ngo","sequence":"additional","affiliation":[{"name":"Preclinical Imaging, Department of Radiological Sciences, University of California-Irvine, Irvine, CA 92697, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6541-9842","authenticated-orcid":false,"given":"Titus E.","family":"Tucker","sequence":"additional","affiliation":[{"name":"Preclinical Imaging, Department of Radiological Sciences, University of California-Irvine, Irvine, CA 92697, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2810-766X","authenticated-orcid":false,"given":"Ashlee D. 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