{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T16:55:31Z","timestamp":1766076931647,"version":"3.48.0"},"reference-count":48,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T00:00:00Z","timestamp":1766016000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32273111"],"award-info":[{"award-number":["32273111"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32271329"],"award-info":[{"award-number":["32271329"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32071262"],"award-info":[{"award-number":["32071262"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Science and Technology Innovation Program of Hunan Province","award":["2020RC4023"],"award-info":[{"award-number":["2020RC4023"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Numerous modern scientific studies have demonstrated that animal venoms harbor a wealth of diverse anticancer active components, serving as a valuable resource for the development of natural antitumor drugs. AI-based computation and prediction models enable rapid screening of extensive active peptides. In this study, the anticancer activity of seven peptides was predicted using our previous deep learning model. Further verification experiments confirmed that Lpep3 can selectively and efficiently inhibit the growth of leukemia cells. Electron microscopy observations revealed cell shrinkage in morphology and honeycomb-like perforations on the cell membrane in the treated group. It is hypothesized that high-concentration peptides disrupt the cell membrane and increase cell permeability, which was confirmed by trypan blue staining and Calcein-AM\/PI double-staining assays. Lpep3 induces the release of lactate dehydrogenase (LDH) and ATP in a concentration-dependent manner, further suggesting that this peptide disrupts the cell membrane. In addition, although Lpep3 does not affect the cell cycle of MV-4-11, it can induce cell apoptosis. Western blotting and RT-qPCR results showed that compared with the control group, the expression levels of Bax were upregulated, while the expression level of Bcl-2 protein was downregulated in the Lpep3 group. In vivo experiments demonstrated that Lpep3 has good biological safety, and compared with the control group, the Lpep3 group could inhibit the growth of tumor cells in mice. Collectively, Lpep3 is characterized by high potency and specificity and may serve as a promising lead compound for the development of anti-leukemia drugs.<\/jats:p>","DOI":"10.3390\/biom15121751","type":"journal-article","created":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T16:42:05Z","timestamp":1766076125000},"page":"1751","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Scorpion Peptide Exerts Selective Anti-Leukemia Effects Through Disrupting Cell Membranes and Triggering Bax\/Bcl-2-Related Apoptosis Pathway"],"prefix":"10.3390","volume":"15","author":[{"given":"Xiaoping","family":"Dong","sequence":"first","affiliation":[{"name":"National & Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, China"},{"name":"Peptide and Small Molecule Drug R&D Platform, Furong Laboratory, Hunan Normal University, Changsha 410081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siwei","family":"Yi","sequence":"additional","affiliation":[{"name":"National & Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, China"},{"name":"Peptide and Small Molecule Drug R&D Platform, Furong Laboratory, Hunan Normal University, Changsha 410081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaqin","family":"Yang","sequence":"additional","affiliation":[{"name":"National & Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, China"},{"name":"Peptide and Small Molecule Drug R&D Platform, Furong Laboratory, Hunan Normal University, Changsha 410081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yushuo","family":"Wang","sequence":"additional","affiliation":[{"name":"National & Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, China"},{"name":"Peptide and Small Molecule Drug R&D Platform, Furong Laboratory, Hunan Normal University, Changsha 410081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingxiang","family":"Wang","sequence":"additional","affiliation":[{"name":"National & Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, China"},{"name":"Peptide and Small Molecule Drug R&D Platform, Furong Laboratory, Hunan Normal University, Changsha 410081, China"},{"name":"The State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha 410081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingjing","family":"Huang","sequence":"additional","affiliation":[{"name":"National & Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, China"},{"name":"Peptide and Small Molecule Drug R&D Platform, Furong Laboratory, Hunan Normal University, Changsha 410081, China"},{"name":"The State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha 410081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0119-2852","authenticated-orcid":false,"given":"Yong","family":"Zeng","sequence":"additional","affiliation":[{"name":"National & Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, China"},{"name":"Peptide and Small Molecule Drug R&D Platform, Furong Laboratory, Hunan Normal University, Changsha 410081, China"},{"name":"The State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha 410081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0000-4565","authenticated-orcid":false,"given":"Zhonghua","family":"Liu","sequence":"additional","affiliation":[{"name":"National & Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, China"},{"name":"Peptide and Small Molecule Drug R&D Platform, Furong Laboratory, Hunan Normal University, Changsha 410081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/j.suronc.2018.07.011","article-title":"Comparative effectiveness of preoperative, postoperative and perioperative treatments for resectable gastric cancer: A network meta-analysis of the literature from the past 20 years","volume":"27","author":"Cai","year":"2018","journal-title":"Surg. Oncol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.canlet.2019.02.048","article-title":"Mechanisms and therapeutic potentials of cancer immunotherapy in combination with radiotherapy and\/or chemotherapy","volume":"452","author":"Yu","year":"2019","journal-title":"Cancer Lett."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"170106","DOI":"10.1016\/j.peptides.2019.170106","article-title":"Structural analysis and mode of action of BMAP-27, a cathelicidin-derived antimicrobial peptide","volume":"118","author":"Yang","year":"2019","journal-title":"Peptides"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1034\/j.1399-3011.2001.00934.x","article-title":"Antibacterial, antitumor and hemolytic activities of alpha-helical antibiotic peptide, P18 and its analogs","volume":"58","author":"Shin","year":"2001","journal-title":"J. Pept. Res."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Wang, A., Zheng, Y., Zhu, W., Yang, L., Yang, Y., and Peng, J. (2022). Melittin-Based Nano-Delivery Systems for Cancer Therapy. Biomolecules, 12.","DOI":"10.3390\/biom12010118"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Varricchi, G., Ameri, P., Cadeddu, C., Ghigo, A., Madonna, R., Marone, G., Mercurio, V., Monte, I., Novo, G., and Parrella, P. (2018). Antineoplastic Drug-Induced Cardiotoxicity: A Redox Perspective. Front. Physiol., 9.","DOI":"10.3389\/fphys.2018.00167"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.peptides.2019.02.009","article-title":"Peptides with therapeutic potential in the venom of the scorpion Buthus martensii Karsch","volume":"115","author":"Li","year":"2019","journal-title":"Peptides"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4234273","DOI":"10.1155\/2020\/4234273","article-title":"The Pivotal Potentials of Scorpion Buthus Martensii Karsch-Analgesic-Antitumor Peptide in Pain Management and Cancer","volume":"2020","author":"Richard","year":"2020","journal-title":"Evid. Based Complement. Alternat Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e822","DOI":"10.1002\/ctm2.822","article-title":"The role of cell-penetrating peptides in potential anti-cancer therapy","volume":"12","author":"Zhou","year":"2022","journal-title":"Clin. Transl. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"7807","DOI":"10.7150\/thno.37194","article-title":"Peptide-based materials for cancer immunotherapy","volume":"9","author":"Zhang","year":"2019","journal-title":"Theranostics"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1038\/s41573-020-00135-8","article-title":"Trends in peptide drug discovery","volume":"20","author":"Muttenthaler","year":"2021","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e046588","DOI":"10.1136\/bmjopen-2020-046588","article-title":"A phase 3 randomised study of enzalutamide plus leuprolide and enzalutamide monotherapy in high-risk non-metastatic hormone-sensitive prostate cancer with rising PSA after local therapy: EMBARK study design","volume":"11","author":"Freedland","year":"2021","journal-title":"BMJ Open"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e939","DOI":"10.1016\/j.clbc.2018.04.005","article-title":"Efficacy of Different Leuprolide Administration Schedules in Premenopausal Breast Cancer: A Retrospective Review","volume":"18","author":"Kendzierski","year":"2018","journal-title":"Clin. Breast Cancer"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"189197","DOI":"10.1016\/j.bbcan.2024.189197","article-title":"Advancements in therapeutic peptides: Shaping the future of cancer treatment","volume":"1879","author":"Chen","year":"2024","journal-title":"Biochim. Biophys. Acta Rev. Cancer"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1039\/D0NP00019A","article-title":"Cytotoxic and antitumor peptides as novel chemotherapeutics","volume":"38","author":"Luan","year":"2021","journal-title":"Nat. Prod. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1464","DOI":"10.1007\/s00134-017-4878-x","article-title":"Antimicrobial resistance in the next 30 years, humankind, bugs and drugs: A visionary approach","volume":"43","author":"Bassetti","year":"2017","journal-title":"Intensive Care Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"106978","DOI":"10.1016\/j.phrs.2023.106978","article-title":"Scorpion venom peptides: Molecular diversity; structural characteristics, and therapeutic use from channelopathies to viral infections and cancers","volume":"197","author":"Xia","year":"2023","journal-title":"Pharmacol. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"bbab008","DOI":"10.1093\/bib\/bbab008","article-title":"Anticancer peptides prediction with deep representation learning features","volume":"22","author":"Lv","year":"2021","journal-title":"Brief. Bioinform."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4941","DOI":"10.1021\/acs.jcim.4c00295","article-title":"Advancing Peptide-Based Cancer Therapy with AI: In-Depth Analysis of State-of-the-Art AI Models","volume":"64","author":"Bhattarai","year":"2024","journal-title":"J. Chem. Inf. Model."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"6174","DOI":"10.1021\/acs.jcim.4c00501","article-title":"Identification of Anticancer Peptides from the Genome of Candida albicans: In Silico Screening, in Vitro and in Vivo Validations","volume":"64","author":"Cheong","year":"2024","journal-title":"J. Chem. Inf. Model."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Li, Y., Ma, D., Chen, D., and Chen, Y. (2023). ACP-GBDT: An improved anticancer peptide identification method with gradient boosting decision tree. Front. Genet., 14.","DOI":"10.3389\/fgene.2023.1165765"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"102349","DOI":"10.1016\/j.artmed.2022.102349","article-title":"cACP-DeepGram: Classification of anticancer peptides via deep neural network and skip-gram-based word embedding model","volume":"131","author":"Akbar","year":"2022","journal-title":"Artif. Intell. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"138880","DOI":"10.1016\/j.ijbiomac.2024.138880","article-title":"Discovery of anticancer peptides from natural and generated sequences using deep learning","volume":"290","author":"Yue","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/978-1-0716-3914-6_3","article-title":"Fmoc Solid-Phase Peptide Synthesis","volume":"2821","author":"Hansen","year":"2024","journal-title":"Methods Mol. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"669","DOI":"10.2217\/imt-2020-0312","article-title":"Antimicrobial\/anticancer peptides: Bioactive molecules and therapeutic agents","volume":"13","author":"Kardani","year":"2021","journal-title":"Immunotherapy"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1016\/j.biopha.2016.10.041","article-title":"The scorpion venom peptide BmKn2 induces apoptosis in cancerous but not in normal human oral cells","volume":"84","author":"Satitmanwiwat","year":"2016","journal-title":"Biomed. Pharmacother."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1784","DOI":"10.1016\/j.biochi.2013.06.003","article-title":"Two peptides; TsAP-1; TsAP-2, from the venom of the Brazilian yellow scorpion, Tityus serrulatus: Evaluation of their antimicrobial and anticancer activities","volume":"95","author":"Guo","year":"2013","journal-title":"Biochimie"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1126\/science.aay9189","article-title":"The human tumor microbiome is composed of tumor type-specific intracellular bacteria","volume":"368","author":"Nejman","year":"2020","journal-title":"Science"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"173317","DOI":"10.1016\/j.ejphar.2020.173317","article-title":"Cecropins in cancer therapies-where we have been?","volume":"882","author":"Ziaja","year":"2020","journal-title":"Eur. J. Pharmacol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"105231","DOI":"10.1016\/j.phrs.2020.105231","article-title":"Cell-penetrating peptides in oncologic pharmacotherapy: A review","volume":"162","author":"Vale","year":"2020","journal-title":"Pharmacol. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1038\/s41698-020-00129-0","article-title":"Honeybee venom and melittin suppress growth factor receptor activation in HER2-enriched and triple-negative breast cancer","volume":"4","author":"Duffy","year":"2020","journal-title":"NPJ Precis. Oncol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Casanova, Y.V., Guerra, J.A.R., Perez, Y.A.U., Castro, A.L.L., Reina, G.A., Castaneda, J.E.G., and Monroy, Z.J.R. (2017). Antibacterial Synthetic Peptides Derived from Bovine Lactoferricin Exhibit Cytotoxic Effect against MDA-MB-468 and MDA-MB-231 Breast Cancer Cell Lines. Molecules, 22.","DOI":"10.3390\/molecules22101641"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3843","DOI":"10.2147\/IJN.S76104","article-title":"Magnetic nanoparticles enhance the anticancer activity of cathelicidin LL-37 peptide against colon cancer cells","volume":"10","author":"Niemirowicz","year":"2015","journal-title":"Int. J. Nanomed."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1177\/0022034516679973","article-title":"Antimicrobial Peptides: Mechanisms of Action and Resistance","volume":"96","author":"Bechinger","year":"2017","journal-title":"J. Dent. Res."},{"key":"ref_35","first-page":"3052","article-title":"Anticancer efficacy of Magainin2 and analogue peptides","volume":"53","author":"Baker","year":"1993","journal-title":"Cancer Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"5330","DOI":"10.1046\/j.1432-1327.2000.01536.x","article-title":"The antibiotic and anticancer active aurein peptides from the Australian Bell Frogs Litoria aurea and Litoria raniformis the solution structure of aurein 1.2","volume":"267","author":"Rozek","year":"2000","journal-title":"Eur. J. Biochem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1046\/j.1432-1033.2003.03462.x","article-title":"nNOS inhibition, antimicrobial and anticancer activity of the amphibian skin peptide, citropin 1.1 and synthetic modifications. The solution structure of a modified citropin 1.1","volume":"270","author":"Doyle","year":"2003","journal-title":"Eur. J. Biochem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.toxicon.2019.06.016","article-title":"Buforin IIb induces androgen-independent prostate cancer cells apoptosis though p53 pathway in vitro","volume":"168","author":"Han","year":"2019","journal-title":"Toxicon"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Mader, J.S., Mookherjee , N., Hancock , R.E., and Bleackley , R.C. (2009). The human host defense peptide LL-37 induces apoptosis in a calpain- and apoptosis-inducing factor-dependent manner involving Bax activity. Mol. Cancer Res., 5.","DOI":"10.1158\/1541-7786.MCR-08-0274"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1080\/17460441.2023.2157402","article-title":"Tachyplesin I and its derivatives: A pharmaco-chemical perspective on their antimicrobial and antitumor potential","volume":"17","author":"Lu","year":"2022","journal-title":"Expert. Opin. Drug Discov."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2424","DOI":"10.1002\/jcb.23166","article-title":"Analgesic-antitumor peptide inhibits proliferation and migration of SHG-44 human malignant glioma cells","volume":"112","author":"Zhao","year":"2011","journal-title":"J. Cell Biochem."},{"key":"ref_42","first-page":"1305","article-title":"Novel walnut peptide-selenium hybrids with enhanced anticancer synergism: Facile synthesis and mechanistic investigation of anticancer activity","volume":"11","author":"Liao","year":"2016","journal-title":"Int. J. Nanomed."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"112676","DOI":"10.1016\/j.biopha.2022.112676","article-title":"Recent advances and limitations in the application of kahalalides for the control of cancer","volume":"148","author":"Wyer","year":"2022","journal-title":"Biomed. Pharmacother."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"9208","DOI":"10.1021\/bi0201466","article-title":"Targeting of nonkaryophilic cell-permeable peptides into the nuclei of intact cells by covalently attached nuclear localization signals","volume":"41","author":"Feder","year":"2002","journal-title":"Biochemistry"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Li, X.Q., Yamazaki, T., He, T., Alam, M.M., Liu, J., Trivett, A.L., Sveinbjornsson, B., Rekdal, O., Galluzzi, L., and Oppenheim, J.J. (2024). LTX-315 triggers anticancer immunity by inducing MyD88-dependent maturation of dendritic cells. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1332922"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"10151","DOI":"10.1016\/j.biomaterials.2013.09.041","article-title":"A cancer vaccine based on the marine antimicrobial peptide pardaxin (GE33) for control of bladder-associated tumors","volume":"34","author":"Huang","year":"2013","journal-title":"Biomaterials"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2505","DOI":"10.4155\/fmc-2019-0100","article-title":"A small cytotoxic peptide from frog elicits potent antitumor immunity to prevent local tumor growth and metastases","volume":"11","author":"Qiao","year":"2019","journal-title":"Future Med. Chem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"8568","DOI":"10.1039\/D3CC01867A","article-title":"An injectable nanocomposite alginate-Ca2+ hydrogel for melittin-assisted Ca2+-overload and photothermal cancer therapy","volume":"59","author":"Yang","year":"2023","journal-title":"Chem. Commun."}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/15\/12\/1751\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T16:51:04Z","timestamp":1766076664000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/15\/12\/1751"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,18]]},"references-count":48,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["biom15121751"],"URL":"https:\/\/doi.org\/10.3390\/biom15121751","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,18]]}}}