{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T22:33:37Z","timestamp":1775514817617,"version":"3.50.1"},"reference-count":55,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,25]],"date-time":"2025-12-25T00:00:00Z","timestamp":1766620800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100019731","name":"Department of Cytology, Histology and Reproductive Medicine of the Educational and Scientific Centre \u201cInstitute of Biology and Medicine\u201d of Taras Shevchenko National University of Kyiv","doi-asserted-by":"publisher","award":["24KF036-07"],"award-info":[{"award-number":["24KF036-07"]}],"id":[{"id":"10.13039\/501100019731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association (AHA) International Visiting Professorship Award","doi-asserted-by":"publisher","award":["24IVPHA1293598"],"award-info":[{"award-number":["24IVPHA1293598"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"name":"AHA Strategically Focused Network on CKMS"},{"name":"AHA Transformational Project Award"},{"DOI":"10.13039\/100000968","name":"AHA Career Development Award","doi-asserted-by":"publisher","award":["25CDA1449142"],"award-info":[{"award-number":["25CDA1449142"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"name":"AHA Predoctoral Fellowship","award":["25PRE1372769"],"award-info":[{"award-number":["25PRE1372769"]}]},{"name":"AU Physiology startup funds"},{"name":"Dialysis Clinic, Inc. Paul Teschan Research Fund"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Obesity is a major contributor to kidney injury, in part through circadian rhythms disruption and oxidative stress. Melatonin, a circadian clock regulator, has been proposed as a protective agent against metabolic and renal complications. We investigated the effects of chronic melatonin supplementation on kidney injury and circadian regulation in a rat obesity model. We hypothesized that melatonin administration ameliorates kidney injury induced by a high-calorie diet. Male Wistar rats were fed a normal or hypercaloric diet for six weeks, followed by seven weeks of vehicle or melatonin treatment (30 mg\/kg\/day in drinking water); biometric parameters and renal injury were assessed. Obese rats exhibited increased visceral adiposity, elevated resistin, renal hypertrophy, fibrosis, tubular degeneration, and glomerular injury, accompanied by higher KIM-1 levels. Melatonin attenuated renal fibrosis, reduced KIM-1, TGF\u03b2, and TNFR1 levels, improved proximal tubule and glomerular damage, and lowered adipose TNF-\u03b1 levels in the obese groups. In lean controls, melatonin increased nuclear BMAL1 levels, while in obese rats this effect was blunted; of note, BMAL1 accumulated in distal tubular cytoplasm in both melatonin-treated groups. These findings suggest that melatonin mitigates obesity-induced renal pathology through anti-fibrotic inflammation-related mechanisms, while also revealing a novel link between circadian disruption and kidney injury. Our results support melatonin as a therapeutic agent for obesity-related renal disease.<\/jats:p>","DOI":"10.3390\/biom16010036","type":"journal-article","created":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T02:07:58Z","timestamp":1766714878000},"page":"36","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Melatonin Administration Attenuates High-Fat-Diet-Induced Renal Damage in Wistar Rats"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5025-0938","authenticated-orcid":false,"given":"Olesia","family":"Kalmukova","sequence":"first","affiliation":[{"name":"Educational and Scientific Center \u201cInstitute of Biology and Medicine\u201d, Taras Shevchenko National University of Kyiv, 03022 Kyiv, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0377-972X","authenticated-orcid":false,"given":"Anastasiia","family":"Zavora","sequence":"additional","affiliation":[{"name":"Department of Physiology, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA"},{"name":"Academic and Research Medical Institute, Sumy State University, 40000 Sumy, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alena","family":"Cherezova","sequence":"additional","affiliation":[{"name":"Department of Physiology, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3621-6981","authenticated-orcid":false,"given":"Olexiy","family":"Savchuk","sequence":"additional","affiliation":[{"name":"Educational and Scientific Center \u201cInstitute of Biology and Medicine\u201d, Taras Shevchenko National University of Kyiv, 03022 Kyiv, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2161-2842","authenticated-orcid":false,"given":"Mariia","family":"Stefanenko","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mykhailo","family":"Fedoriuk","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam C.","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Physiology, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentyn","family":"Nepomnyashchy","sequence":"additional","affiliation":[{"name":"Department of Toxicology, State Institute of Pharmacology and Toxicology, The National Academy of Medical Sciences of Ukraine, 03057 Kyiv, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mykola","family":"Dzerzhynskyi","sequence":"additional","affiliation":[{"name":"Educational and Scientific Center \u201cInstitute of Biology and Medicine\u201d, Taras Shevchenko National University of Kyiv, 03022 Kyiv, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4590-3193","authenticated-orcid":false,"given":"Marharyta","family":"Semenikhina","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daria V.","family":"Ilatovskaya","sequence":"additional","affiliation":[{"name":"Department of Physiology, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3680-5527","authenticated-orcid":false,"given":"Oleg","family":"Palygin","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1038\/s41574-018-0130-1","article-title":"Melatonin in type 2 diabetes mellitus and obesity","volume":"15","author":"Karamitri","year":"2019","journal-title":"Nat. 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