{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T15:05:33Z","timestamp":1789571133051,"version":"build-2803163510"},"reference-count":61,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T00:00:00Z","timestamp":1767744000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R15GM152920"],"award-info":[{"award-number":["R15GM152920"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R15GM137291"],"award-info":[{"award-number":["R15GM137291"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>N6-methyladenosine (m6A) constitutes the most prevalent nucleotide modification within eukaryotic messenger RNA (mRNA). Variations in m6A levels are associated with numerous human diseases and health conditions, including various forms of cancer, diabetes, neurological disorders, male infertility, and obesity. Nevertheless, the molecular mechanisms underpinning the recognition of m6A by different \u2018reader\u2019 proteins remain incompletely elucidated. In this study, we used phage display to identify key sequence features that methyl readers recognize in m6A. This study shows that m6A modifications affect the mRNA interactome. A peptide motif recognizing m6A in DRACH sequences suggests a common recognition mechanism, though proteins may use different methods to detect m6A in less accessible areas. The sequence of the hnRNP A1 RRM domain that aligns with the newly discovered m6A-binding peptide, m1p1, is crucial for the binding of m6A-modified RNAs, indicating a strong link between the m1p1 sequence and m6A recognition, which is key for recognizing m6A-modified, unstructured RNAs. Gaining a comprehensive understanding of the evolutionary influence of m6A on its reader proteins may facilitate the identification of additional m6A readers. These signature peptides could enhance theranostic approaches across cancers, enabling more targeted therapies.<\/jats:p>","DOI":"10.3390\/biom16010097","type":"journal-article","created":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T09:34:12Z","timestamp":1767778452000},"page":"97","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Identification of Key Sequence Motifs Essential for the Recognition of m6A Modification in RNA"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-8601-5984","authenticated-orcid":false,"given":"Aftab","family":"Mollah","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, Kent State University, Kent, OH 44242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rushdhi","family":"Rauff","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Kent State University, Kent, OH 44242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudeshi","family":"Abedeera","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Kent State University, Kent, OH 44242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3053-9127","authenticated-orcid":false,"given":"Chathurani","family":"Ekanayake","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Kent State University, Kent, OH 44242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chamali Thalagaha","family":"Thalagaha Mudiyanselage","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Kent State University, Kent, OH 44242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minhchau","family":"To","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Kent State University, Kent, OH 44242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1844-864X","authenticated-orcid":false,"given":"Helen","family":"Piontkivska","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Kent State University, Kent, OH 44242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2428-1962","authenticated-orcid":false,"given":"Sanjaya","family":"Abeysirigunawardena","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Kent State University, Kent, OH 44242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,7]]},"reference":[{"key":"ref_1","first-page":"425","article-title":"Cell Differentiation and Aging Lead to Up-Regulation of FTO, While the ALKBH5 Protein Level Was Stable during Aging but up-Regulated during in Vitro-Induced Cardiomyogenesis","volume":"72","author":"Arcidiacono","year":"2023","journal-title":"Physiol. 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