{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T11:58:23Z","timestamp":1772107103412,"version":"3.50.1"},"reference-count":55,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T00:00:00Z","timestamp":1772064000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Medical University of Silesia","award":["BNW-2-051\/K\/4\/F"],"award-info":[{"award-number":["BNW-2-051\/K\/4\/F"]}]},{"name":"The Medical University of Silesia","award":["BNW-1-048\/K\/5\/F"],"award-info":[{"award-number":["BNW-1-048\/K\/5\/F"]}]},{"name":"The Medical University of Silesia","award":["BNW-1-105\/K\/4\/F"],"award-info":[{"award-number":["BNW-1-105\/K\/4\/F"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Epigenetic and stress-response pathways play central roles in cancer progression and represent attractive therapeutic targets. In this study, a series of dipyridothiazine\u20131,2,3-triazole hybrids bearing p-fluorophenyl and p-trifluoromethylphenyl substituents was synthesized via efficient dipolar cycloaddition reactions. Structural characterization was performed using 1H, 13C, and 19F NMR spectroscopy and high-resolution mass spectrometry. Anticancer activity was evaluated using WST-1 and MTT assays against human cancer cell lines SNB-19 (glioblastoma), C32 (amelanotic melanoma), A549 (lung carcinoma), and MDA-MB-231 and MCF-7 (breast cancer), as well as normal HFF-1 fibroblasts and HaCaT keratinocytes, with doxorubicin and cisplatin as reference drugs. The hybrids TDT2b and TDT3b containing a p-trifluoromethylphenyl moiety showed the highest cytotoxicity and cancer cell selectivity. RT-qPCR analysis of H3, TP53, CDKN1A, BCL-2, and BAX expression for the lead compound TDT2b revealed modulation of chromatin organization, p53-dependent stress responses, apoptosis, and cell cycle regulation. Molecular docking studies with human histone deacetylase 6 (HDAC6) demonstrated favorable binding of TDT2b and TDT3b, supporting their role as potential epigenetic anticancer agents.<\/jats:p>","DOI":"10.3390\/biom16030349","type":"journal-article","created":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T10:33:02Z","timestamp":1772101982000},"page":"349","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["New 1,2,3-Triazole and Dipyridothiazine Hybrids\u2014Synthesis, Analysis, Cytotoxicity and Molecular Docking"],"prefix":"10.3390","volume":"16","author":[{"given":"Emilia","family":"Martula","sequence":"first","affiliation":[{"name":"Department of Organic Chemistry, Faculty of Pharmaceutical Sciences, The Medical University of Silesia, Jagiello\u0144ska 4, 41-200 Sosnowiec, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1749-8514","authenticated-orcid":false,"given":"Weronika","family":"Bagrowska","sequence":"additional","affiliation":[{"name":"Tunneling Group, Biotechnology Centre, Silesian University of Technology, Krzywoustego 8, 44-100 Gliwice, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1830-2210","authenticated-orcid":false,"given":"Paulina","family":"Strzyga-\u0141ach","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Medical University of Warsaw, 02-097 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0181-3607","authenticated-orcid":false,"given":"Marta","family":"Struga","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Medical University of Warsaw, 02-097 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4109-2629","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Latocha","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Faculty of Pharmaceutical Sciences, The Medical University of Silesia, Jedno\u015bci 8, 41-200 Sosnowiec, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dariusz","family":"Ku\u015bmierz","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Faculty of Pharmaceutical Sciences, The Medical University of Silesia, Jedno\u015bci 8, 41-200 Sosnowiec, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4144-990X","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Jele\u0144","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry, Faculty of Pharmaceutical Sciences, The Medical University of Silesia, Jagiello\u0144ska 4, 41-200 Sosnowiec, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5389-1079","authenticated-orcid":false,"given":"Beata","family":"Morak-M\u0142odawska","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry, Faculty of Pharmaceutical Sciences, The Medical University of Silesia, Jagiello\u0144ska 4, 41-200 Sosnowiec, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1353\/pbm.2019.0046","article-title":"What Is Cancer?","volume":"62","author":"Hausman","year":"2019","journal-title":"Perspect. Biol. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1093\/clinchem\/hvad202","article-title":"Epidemiology of Cancer","volume":"70","author":"Schwartz","year":"2024","journal-title":"Clin. Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1016\/S2213-8587(24)00223-7","article-title":"Evolving Epidemiological Patterns of Thyroid Cancer and Estimates of Overdiagnosis in 2013\u201317 in 63 Countries Worldwide: A Population-Based Study","volume":"12","author":"Li","year":"2024","journal-title":"Lancet Diabetes Endocrinol."},{"key":"ref_4","unstructured":"(2026, January 13). Cancer Today. Available online: https:\/\/gco.iarc.who.int\/today\/."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Batool, M., Ahmad, B., and Choi, S. (2019). A Structure-Based Drug Discovery Paradigm. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20112783"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.ejmech.2015.04.051","article-title":"1,3,4-Oxadiazoles: An Emerging Scaffold to Target Growth Factors, Enzymes and Kinases as Anticancer Agents","volume":"97","author":"Bajaj","year":"2015","journal-title":"Eur. J. Med. Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3511","DOI":"10.1016\/j.bmc.2019.07.005","article-title":"1,2,3-Triazole-Containing Hybrids as Leads in Medicinal Chemistry: A Recent Overview","volume":"27","author":"Bozorov","year":"2019","journal-title":"Bioorganic Med. Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1016\/j.ejmech.2017.11.090","article-title":"Synthesis and in Vitro Evaluation of Novel Substituted Isatin-Propylene-1H-1,2,3-Triazole-4-Methylene-Moxifloxacin Hybrids for Their Anti-Mycobacterial Activities","volume":"143","author":"Yan","year":"2018","journal-title":"Eur. J. Med. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3282","DOI":"10.1039\/C7NJ04060A","article-title":"Synthesis, optical properties, and antioxidant and anticancer activity of benzoheterazole dendrimers with triazole bridging unit","volume":"42","author":"Rajavelu","year":"2018","journal-title":"New J. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"16997","DOI":"10.1039\/C8RA03197E","article-title":"1,2,3-Triazole-Fused Spirochromenes as Potential Anti-Tubercular Agents: Synthesis and Biological Evaluation","volume":"8","author":"Ashok","year":"2018","journal-title":"RSC Adv."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1080\/14756366.2022.2072308","article-title":"New 1,2,3-Triazole Linked Ciprofloxacin-Chalcones Induce DNA Damage by Inhibiting Human Topoisomerase I& II and Tubulin Polymerization","volume":"37","author":"Mohammed","year":"2022","journal-title":"J. Enzym. Inhib. Med. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2853","DOI":"10.1016\/j.tetlet.2015.03.136","article-title":"New Click-Chemistry Methods for 1,2,3-Triazoles Synthesis: Recent Advances and Applications","volume":"56","author":"Totobenazara","year":"2015","journal-title":"Tetrahedron Lett."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1039\/D0MD00414F","article-title":"Benzimidazole-1,2,3-Triazole Hybrid Molecules: Synthesis and Study of Their Interaction with G-Quadruplex DNA","volume":"12","author":"Singu","year":"2021","journal-title":"RSC Med. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Azab, I.H.E., El-Sheshtawy, H.S., Bakr, R.B., and Elkanzi, N.A.A. (2021). New 1,2,3-Triazole-Containing Hybrids as Antitumor Candidates: Design, Click Reaction Synthesis, DFT Calculations, and Molecular Docking Study. Molecules, 26.","DOI":"10.3390\/molecules26030708"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4905","DOI":"10.1021\/cr200409f","article-title":"Click Chemistry for Drug Development and Diverse Chemical-Biology Applications","volume":"113","author":"Thirumurugan","year":"2013","journal-title":"Chem. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1007\/s10593-019-02414-8","article-title":"Recent Methods for the Synthesis of NH-1,2,3-Triazoles (Microreview)","volume":"55","author":"Efimov","year":"2019","journal-title":"Chem. Heterocycl. Compd."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e2100158","DOI":"10.1002\/ardp.202100158","article-title":"1,2,3-Triazole Hybrids as Anticancer Agents: A Review","volume":"355","author":"Alam","year":"2022","journal-title":"Arch. Pharm."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1406","DOI":"10.2174\/1568026622666220415225334","article-title":"1,2,3-Triazole Derivatives with Anti-Breast Cancer Potential","volume":"22","author":"Wu","year":"2022","journal-title":"Curr. Top. Med. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e0090724","DOI":"10.1128\/aac.00907-24","article-title":"Clinical Outcomes and Emergence of Resistance of Pseudomonas Aeruginosa Infections Treated with Ceftolozane-Tazobactam versus Ceftazidime-Avibactam","volume":"68","author":"Hareza","year":"2024","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Posso, M.C., Domingues, F.C., Ferreira, S., and Silvestre, S. (2022). Development of Phenothiazine Hybrids with Potential Medicinal Interest: A Review. Molecules, 27.","DOI":"10.3390\/molecules27010276"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.2174\/138945006778226552","article-title":"Phenothiazine: The Parent Molecule","volume":"7","author":"Mitchell","year":"2006","journal-title":"Curr. Drug Targets"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3179","DOI":"10.1016\/j.ejmech.2011.05.013","article-title":"Recent Progress in Biological Activities of Synthesized Phenothiazines","volume":"46","author":"Pluta","year":"2011","journal-title":"Eur. J. Med. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1021\/acs.jmedchem.8b01090","article-title":"Synthesis and Biological Investigation of Phenothiazine-Based Benzhydroxamic Acids as Selective Histone Deacetylase 6 Inhibitors","volume":"62","author":"Ong","year":"2019","journal-title":"J. Med. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"110806","DOI":"10.1016\/j.dyepig.2022.110806","article-title":"Current achievements and perspectives in synthesis and applications of 3,7-disubstituted phenothiazines as Methylene Blue analogues","volume":"208","author":"Padnya","year":"2023","journal-title":"Dye. Pigment."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Martula, E., Morak-M\u0142odawska, B., Jele\u0144, M., Okechukwu, P.N., Balachandran, A., Tehirunavukarasu, P., Anamalay, K., and Ulaganathan, V. (2023). Synthesis and structural characterization of novel dimers of dipyridothiazine as promising antiprolif-erative agents. Molecules, 28.","DOI":"10.3390\/molecules28227662"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1007\/s10637-018-0682-x","article-title":"Antitumor Evaluation of Novel Phenothiazine Derivatives That Inhibit Migration and Tubulin Polymerization against Gastric Cancer MGC-803 Cells","volume":"37","author":"Liu","year":"2019","journal-title":"Investig. New Drugs"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Zhang, J.-X., Guo, J.-M., Zhang, T.-T., Lin, H.-J., Qi, N.-S., Li, Z.-G., Zhou, J.-C., and Zhang, Z.-Z. (2018). Antiproliferative Phenothiazine Hybrids as Novel Apoptosis Inducers against MCF-7 Breast Cancer. Molecules, 23.","DOI":"10.3390\/molecules23061288"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"696","DOI":"10.3184\/174751917X15122516000140","article-title":"Design, Synthesis and Antiproliferative Activity of Novel Phenothiazine-1,2,3-Triazole Analogues","volume":"41","author":"Ma","year":"2017","journal-title":"J. Chem. Res."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Morak-M\u0142odawska, B., Pluta, K., Latocha, M., Jele\u0144, M., and Ku\u015bmierz, D. (2019). Design, Synthesis, and Structural Characterization of Novel Diazaphenothiazines with 1,2,3-Triazole Substituents as Promising Antiproliferative Agents. Molecules, 24.","DOI":"10.3390\/molecules24234388"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.ejmech.2014.10.070","article-title":"6-Substituted 9-Fluoroquino [3,2-b]Benzo[1,4]Thiazines Display Strong Antiproliferative and Antitumor Properties","volume":"89","author":"Pluta","year":"2015","journal-title":"Eur. J. Med. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Giercuszkiewicz-Ha\u015bnik, K., Skonieczna, M., Morak-M\u0142odawska, B., and Jele\u0144, M. (2025). Design, Synthesis, and Testing of 1,2,3-Triazolo-Quinobenzothiazine Hybrids for Cytotoxic and Immunomodulatory Activity. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26146920"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.1007\/s00044-014-1220-9","article-title":"Synthesis and Selected Immunological Properties of 10-Substituted 1,8-Diazaphenothiazines","volume":"24","author":"Pluta","year":"2015","journal-title":"Med. Chem. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.3109\/14756366.2015.1101092","article-title":"Synthesis and Anticancer and Lipophilic Properties of 10-Dialkylaminobutynyl Derivatives of 1,8- and 2,7-Diazaphenothiazines","volume":"31","author":"Pluta","year":"2016","journal-title":"J. Enzym. Inhib. Med. Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2425","DOI":"10.1007\/s00044-016-1646-3","article-title":"Synthesis, Spectroscopic Characterization, and Anticancer Activity of New 10-Substituted 1,6-Diazaphenothiazines","volume":"25","author":"Pluta","year":"2016","journal-title":"Med. Chem. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1512","DOI":"10.3109\/14756366.2016.1151014","article-title":"3,6-Diazaphenothiazines as Potential Lead Molecules\u2014Synthesis, Characterization and Anticancer Activity","volume":"31","author":"Pluta","year":"2016","journal-title":"J. Enzym. Inhib. Med. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1093\/nar\/28.1.235","article-title":"The Protein Data Bank","volume":"28","author":"Berman","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"W537","DOI":"10.1093\/nar\/gks375","article-title":"H++ 3.0: Automating pK Prediction and the Preparation of Biomolecular Structures for Atomistic Molecular Modeling and Simulations","volume":"40","author":"Anandakrishnan","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1021\/acs.jcim.5b00674","article-title":"MCPB.Py: A Python Based Metal Center Parameter Builder","volume":"56","author":"Li","year":"2016","journal-title":"J. Chem. Inf. Model."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"6183","DOI":"10.1021\/acs.jcim.3c01153","article-title":"AmberTools","volume":"63","author":"Case","year":"2023","journal-title":"J. Chem. Inf. Model."},{"key":"ref_41","unstructured":"(2026, January 14). Gaussian 09 (Revision A02). Available online: https:\/\/www.researchgate.net\/publication\/260433987_Gaussian_09_Revision_A02."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"7835","DOI":"10.1021\/acs.jcim.5c01063","article-title":"Recent Developments in Amber Biomolecular Simulations","volume":"65","author":"Case","year":"2025","journal-title":"J. Chem. Inf. Model."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"054123","DOI":"10.1063\/5.0013849","article-title":"A Protocol for Preparing Explicitly Solvated Systems for Stable Molecular Dynamics Simulations","volume":"153","author":"Roe","year":"2020","journal-title":"J. Chem. Phys."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3891","DOI":"10.1021\/acs.jcim.1c00203","article-title":"AutoDock Vina 1.2.0: New Docking Methods, Expanded Force Field, and Python Bindings","volume":"61","author":"Eberhardt","year":"2021","journal-title":"J. Chem. Inf. Model."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2785","DOI":"10.1002\/jcc.21256","article-title":"AutoDock4 and AutoDockTools4: Automated Docking with Selective Receptor Flexibility","volume":"30","author":"Morris","year":"2009","journal-title":"J. Comput. Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"W463","DOI":"10.1093\/nar\/gkaf361","article-title":"PLIP 2025: Introducing Protein-Protein Interactions to the Protein-Ligand Interaction Profiler","volume":"53","author":"Schake","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"ref_47","unstructured":"SwissADME (2026, February 05). SwissDrugDesign. Available online: http:\/\/swissadme.ch."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1038\/s41418-018-0246-9","article-title":"Mutant P53 as a Guardian of the Cancer Cell","volume":"26","author":"Mantovani","year":"2019","journal-title":"Cell Death Differ."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1073\/pnas.0307708100","article-title":"Histone Deacetylase (HDAC) Inhibitor Activation of p21WAF1 Involves Changes in Promoter-Associated Proteins, Including HDAC1","volume":"101","author":"Gui","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1111\/febs.12079","article-title":"Histone Deacetylase 6 Plays a Role as a Distinct Regulator of Diverse Cellular Processes","volume":"280","author":"Li","year":"2013","journal-title":"FEBS J."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"42717","DOI":"10.1038\/srep42717","article-title":"SwissADME: A free web tool to evaluate pharmacokinetics, drug-likeness and medicinalchemistry friendliness of small molecules","volume":"7","author":"Daina","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e202100542","DOI":"10.1002\/cmdc.202100542","article-title":"Recent advances in the prediction of pharmacokinetics properties in drug design studies: A review","volume":"17","author":"Pantaleao","year":"2022","journal-title":"Chem. Med. Chem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0169-409X(96)00423-1","article-title":"Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings","volume":"23","author":"Lipinski","year":"1997","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2615","DOI":"10.1021\/jm020017n","article-title":"Molecular properties that influence the oral bioavailability of drug candidates","volume":"45","author":"Veber","year":"2002","journal-title":"J. Med. Chem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3867","DOI":"10.1021\/jm000292e","article-title":"Prediction of drug absorption using multivariate statistics","volume":"43","author":"Egan","year":"2000","journal-title":"J. Med. Chem."}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/16\/3\/349\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T11:01:56Z","timestamp":1772103716000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/16\/3\/349"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,26]]},"references-count":55,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,3]]}},"alternative-id":["biom16030349"],"URL":"https:\/\/doi.org\/10.3390\/biom16030349","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,26]]}}}