{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:18:13Z","timestamp":1791231493144,"version":"4.1.0"},"reference-count":174,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T00:00:00Z","timestamp":1772496000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001061","name":"Rebecca Cooper Medical Research Foundation","doi-asserted-by":"crossref","award":["F20241364"],"award-info":[{"award-number":["F20241364"]}],"id":[{"id":"10.13039\/501100001061","id-type":"DOI","asserted-by":"crossref"}]},{"name":"NHMRC Ideas","award":["2036837"],"award-info":[{"award-number":["2036837"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Three-dimensional (3D) organoid and co-culture models have emerged as transformative tools for studying human endometrial function, implantation, and placental development, overcoming key limitations of animal and two-dimensional in vitro systems. This review synthesises available information of recent advances in endometrial epithelial organoids (EEOs), trophoblast organoids (TBOs), and increasingly complex co-culture platforms incorporating stromal, vascular, and trophoblast compartments to model epithelial\u2013stromal crosstalk, decidualisation, angiogenesis, and embryo implantation. Emerging developments include assembloid systems, synthetic and semi-synthetic extracellular matrices, and microfluidic organ-on-a-chip technologies that enable long-term culture, hormonal responsiveness, and patient-specific modelling. These approaches have recapitulated key features of the mid-secretory endometrium, placental villous architecture, trophoblast differentiation, and early implantation events while revealing disease-associated dysfunctions in conditions such as endometriosis, adenomyosis, polycystic ovarian syndrome, and endometrial cancer. Despite significant progress, current models remain limited by incomplete cellular diversity, polarity constraints, and challenges in fully modelling immune and vascular interactions. Collectively, emerging 3D organoid and co-culture systems provide physiologically relevant platforms to interrogate human reproductive biology, elucidate mechanisms underlying implantation failure and placental disease, and support the development of personalised therapeutic strategies to improve reproductive outcomes.<\/jats:p>","DOI":"10.3390\/biom16030383","type":"journal-article","created":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T15:22:21Z","timestamp":1772551341000},"page":"383","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Engineering the Human Endometrial\u2013Embryo Interface: Breakthroughs in 3D Uterine Models"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-2783-8112","authenticated-orcid":false,"given":"Jenna A.","family":"Douglas","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Monash University, Clayton, VIC 3168, Australia"},{"name":"The Ritchie Centre, Hudson Institute of Medical Research, Clayton, VIC 3168, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1894-9632","authenticated-orcid":false,"given":"Jordan","family":"Higgins","sequence":"additional","affiliation":[{"name":"Monash Biomedicine Discovery Institute, Department of Anatomy and Developmental Biology, Development and Stem Cells Program, Monash University, Clayton, VIC 3800, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dinasha H.","family":"Wimalasiri","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Monash University, Clayton, VIC 3168, Australia"},{"name":"The Ritchie Centre, Hudson Institute of Medical Research, Clayton, VIC 3168, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7848-1447","authenticated-orcid":false,"given":"Amy L.","family":"Winship","sequence":"additional","affiliation":[{"name":"Monash Biomedicine Discovery Institute, Department of Anatomy and Developmental Biology, Development and Stem Cells Program, Monash University, Clayton, VIC 3800, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harriet C.","family":"Fitzgerald","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Monash University, Clayton, VIC 3168, Australia"},{"name":"The Ritchie Centre, Hudson Institute of Medical Research, Clayton, VIC 3168, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1038\/nrendo.2016.116","article-title":"Fertile ground: Human endometrial programming and lessons in health and disease","volume":"12","author":"Evans","year":"2016","journal-title":"Nat. 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