{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T02:50:11Z","timestamp":1791946211934,"version":"4.3.4"},"reference-count":51,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:00:00Z","timestamp":1778630400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Charles University, Czech Republic"},{"id":[{"id":"https:\/\/ror.org\/024d6js02","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003243","name":"Ministry of Health of the Czech Republic","doi-asserted-by":"crossref","award":["DRO\u2014UHHK 00179906"],"award-info":[{"award-number":["DRO\u2014UHHK 00179906"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Background: This pilot study examined whether oxidative\u2013nitrosative stress is associated with clinical status in amyotrophic lateral sclerosis (ALS). We analyzed associations between plasma markers of oxidative\u2013nitrosative imbalance and ALSFRS\u2013R, disease duration, survival, and routine biochemical parameters. Methods: Twenty-nine ALS patients fulfilling the Gold Coast diagnostic criteria were enrolled. Plasma levels of 3-nitrotyrosine (3\u2013NT), 8-oxo-2\u2032-deoxyguanosine (8\u2013oxodG), malondialdehyde (MDA), glutathione (GSH), non-protein thiols (NP\u2013SH), and non-protein disulfides (NP\u2013SS\u2013NP), as well as creatinine, urea, uric acid and BMI, were measured. Associations with ALSFRS\u2013R and disease duration were evaluated using non-parametric correlation analyses and second-order polynomial regression (adjusted R2), while survival was explored using Kaplan\u2013Meier analysis and multivariable Cox regression. Given the modest sample, we considered statistical power and applied Benjamini\u2013Hochberg false discovery rate (FDR) correction within marker families. Results: At the uncorrected significance level, 3\u2013NT showed a positive correlation with ALSFRS\u2013R and a negative correlation with disease duration, and NP\u2013SH correlated negatively with disease duration; however, these associations did not remain significant after FDR correction (FDR-adjusted p \u2265 0.099). Other oxidative\u2013nitrosative markers and biochemical parameters showed no robust relationships with clinical measures. In Cox models, 3\u2013NT was not significantly associated with survival (HR 3.44 per 1 nM, 95% CI 0.25\u201347.97, p = 0.358), whereas older age predicted higher mortality (HR 1.05 per year, 95% CI 1.00\u20131.10, p = 0.036). Conclusions: 3\u2013NT and NP\u2013SH exhibited the strongest trends among the investigated markers, but their clinical associations in this small cross-sectional cohort remain exploratory and require confirmation in larger longitudinal studies.<\/jats:p>","DOI":"10.3390\/biom16050721","type":"journal-article","created":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T16:46:18Z","timestamp":1779381978000},"page":"721","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Oxidative\u2013Nitrosative Stress and Routine Biochemical Parameters in Amyotrophic Lateral Sclerosis: Associations with Clinical Status and Disease Duration\u2014A Pilot Study"],"prefix":"10.3390","volume":"16","author":[{"given":"Pavl\u00edna","family":"Mal\u00e1","sequence":"first","affiliation":[{"name":"Department of Neurology, University Hospital Hradec Kr\u00e1lov\u00e9, 500 05 Hradec Kr\u00e1lov\u00e9, Czech Republic"},{"name":"Department of Neurology, Faculty of Medicine in Hradec Kr\u00e1lov\u00e9, Charles University, 500 03 Hradec Kr\u00e1lov\u00e9, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nela","family":"V\u00e1\u0148ov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy in Hradec Kr\u00e1lov\u00e9, Charles University, 500 05 Hradec Kr\u00e1lov\u00e9, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1287-0519","authenticated-orcid":false,"given":"Ond\u0159ej","family":"Mal\u00fd","sequence":"additional","affiliation":[{"name":"Military Faculty of Medicine, University of Defense, 500 01 Hradec Kr\u00e1lov\u00e9, Czech Republic"},{"name":"Department of Surgery, University Hospital Hradec Kr\u00e1lov\u00e9, 500 05 Hradec Kr\u00e1lov\u00e9, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Old\u0159ich","family":"Vy\u0161ata","sequence":"additional","affiliation":[{"name":"Department of Neurology, University Hospital Hradec Kr\u00e1lov\u00e9, 500 05 Hradec Kr\u00e1lov\u00e9, Czech Republic"},{"name":"Department of Neurology, Faculty of Medicine in Hradec Kr\u00e1lov\u00e9, Charles University, 500 03 Hradec Kr\u00e1lov\u00e9, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"17071","DOI":"10.1038\/nrdp.2017.71","article-title":"Amyotrophic lateral sclerosis","volume":"3","author":"Hardiman","year":"2017","journal-title":"Nat. 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