{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T11:08:19Z","timestamp":1779448099109,"version":"3.53.1"},"reference-count":84,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:00:00Z","timestamp":1779148800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Meningiomas are the most common intracranial tumours, yet the molecular programs underlying WHO grade 1 subtypes\u2014particularly transitional diploid tumours\u2014remain insufficiently defined, partly due to the scarcity of biologically faithful in vitro models. Here, we report the establishment of a long-term, genetically unmanipulated grade 1 meningioma cell line (H8T-MG) maintained under normoxic conditions in serum-containing, growth-factor-supplemented medium, together with a complementary long-term primary culture (H16T-MG), and provide an integrated descriptive and functional characterization of these models, combined with a subtype-restricted transcriptomic analysis of diploid transitional grade 1 tumours versus normal meninges. Both cultures preserved the dual meso-neuroectodermal identity characteristic of meningothelial cells, exhibiting stable adherent growth, preserved contact inhibition and a coherent immunocytochemical profile, expressing vimentin, \u03b1-SMA, nestin, connexin-43 and cannabinoid receptors\u2014reported here for the first time in grade 1 meningioma cultures\u2014highlighting cannabinoid-related pathways as potential targets for exploration. Transcriptomic analysis identified 51 differentially expressed genes, revealing a coherent inflammatory\u2013metabolic programme characterised by downregulation of IL-17 and TNF signalling, cytokines and chemokines (IL6, CCL2, SELE, S100A8), together with reduced extracellular-matrix and cytoskeletal activity. In parallel, the enrichment of arachidonic acid metabolism, cytochrome-P450\/xenobiotic pathways, retinol metabolism and oxidative\/epoxygenase activity indicated a lipid\/xenobiotic-oriented metabolic shift distinctive of this subtype. Protein\u2013protein interaction analysis identified four hub genes\u2014ASPN, SELE, ACKR1 and ABCB1\u2014integrating ECM remodelling, endothelial\u2013immune modulation and xenobiotic transport, reinforcing an immune-attenuated, metabolically adapted tumour landscape. Collectively, these findings provide the first integrated in vitro and transcriptomic characterisation of diploid transitional meningiomas, underscore the value of biologically stable models for early-stage meningioma research, and support the value of histological and ploidy stratification in grade 1 meningioma biology.<\/jats:p>","DOI":"10.3390\/biom16050744","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T10:07:47Z","timestamp":1779444467000},"page":"744","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishment of the H8T-MG Meningioma Cell Line and Integrated Transcriptomics Reveal a Metabolic\u2013Immune Signature in Diploid Transitional WHO Grade 1 Tumours"],"prefix":"10.3390","volume":"16","author":[{"given":"Esther","family":"Manche\u00f1o-Maci\u00e1","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, School of Health Sciences, Universidad Cardenal Herrera-CEU, CEU Universities, 03204 Elche, Alicante, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Leal-Clavel","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, School of Health Sciences, Universidad Cardenal Herrera-CEU, CEU Universities, 03204 Elche, Alicante, Spain"},{"name":"Department of Nursing and Physiotherapy, School of Health Sciences, Universidad Cardenal Herrera-CEU, CEU Universities, 03204 Elche, Alicante, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7261-2458","authenticated-orcid":false,"given":"Vanesa","family":"Escudero-Ortiz","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, School of Health Sciences, Universidad Cardenal Herrera-CEU, CEU Universities, 03204 Elche, Alicante, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"iv1","DOI":"10.1093\/neuonc\/noaa200","article-title":"CBTRUS Statistical Report: Primary brain and other central nervous system tumors diagnosed in the United States in 2013\u20132017","volume":"22","author":"Ostrom","year":"2020","journal-title":"Neuro Oncol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1007\/s11060-010-0342-2","article-title":"Pathological classification and molecular genetics of meningiomas","volume":"99","author":"Mawrin","year":"2010","journal-title":"J. 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