{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:35:18Z","timestamp":1785429318995,"version":"3.56.0"},"reference-count":55,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T00:00:00Z","timestamp":1783123200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Aging","doi-asserted-by":"publisher","award":["5R01AG056504-02"],"award-info":[{"award-number":["5R01AG056504-02"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Aging","doi-asserted-by":"publisher","award":["1R56AG049083-01"],"award-info":[{"award-number":["1R56AG049083-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Aging","doi-asserted-by":"publisher","award":["5P01AG039355-12"],"award-info":[{"award-number":["5P01AG039355-12"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Trauma Research and Combat Casualty Care Collaborative","award":["TRC4-2024-0000000031"],"award-info":[{"award-number":["TRC4-2024-0000000031"]}]},{"name":"George W. and Hazel M. Jay Research Endowments"},{"id":[{"id":"https:\/\/ror.org\/04h0zjx60","id-type":"ROR","asserted-by":"publisher"}]},{"name":"UTSW-NCATs","award":["#1UL1TR003163-04"],"award-info":[{"award-number":["#1UL1TR003163-04"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Sarcopenia is characterized by progressive loss of skeletal muscle mass and function and is a major contributor to frailty, disability, and mortality in older adults. Store-operated calcium entry (SOCE) is a crucial regulator of skeletal muscle calcium homeostasis, and impaired SOCE has been linked to age-related muscle weakness. Here, we identify the synaptophysin family member synaptophysin-like protein 2, also known as mitsugumin 29 (MG29; encoded by the human gene SYPL2 and the mouse ortholog Mg29), as a key organizer of triad membrane cholesterol and lipid signaling required for normal SOCE during aging. Using Mg29\u2212\/\u2212 mice as a model of accelerated sarcopenia, together with RNA interference against Mg29 in adult muscle and primary myotubes, we quantified changes in muscle morphology, contractile function, SOCE activity, and targeted lipidomic profiles. Reduced MG29 expression led to decreased muscle fiber cross-sectional area, reduced specific force, blunted SOCE, and marked alterations in membrane cholesterol content and fatty acid-derived lipid mediators. Cholesterol depletion by methyl-\u03b2-cyclodextrin in wild-type myotubes produced SOCE defects similar to those observed in aged wild-type and young Mg29\u2212\/\u2212 muscles, indicating that MG29-dependent maintenance of membrane cholesterol is required for normal SOCE. Acute Mg29 knockdown also altered myogenic differentiation, the expression of calcium-handling and stress-response genes, and the release and consumption of specific polyunsaturated fatty acid-derived lipid mediators. Together, these findings identify MG29 as a critical regulator of SOCE and lipid signaling in skeletal muscle and suggest that its age-related decline contributes to sarcopenia by disrupting triad membrane organization and excitation\u2013contraction coupling.<\/jats:p>","DOI":"10.3390\/biom16070988","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T14:56:42Z","timestamp":1783349802000},"page":"988","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Reduced Synaptophysin-like 2 (MG29\/SYPL2) Levels Mimic Age-Related Alterations in Skeletal Muscle Calcium Homeostasis and Lipid Signaling"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3621-1136","authenticated-orcid":false,"given":"Kamal","family":"Awad","sequence":"first","affiliation":[{"name":"Bone-Muscle Research Center, College of Nursing and Health Innovation, The University of Texas at Arlington, Arlington, TX 76019, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Huang","sequence":"additional","affiliation":[{"name":"Bone-Muscle Research Center, College of Nursing and Health Innovation, The University of Texas at Arlington, Arlington, TX 76019, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marian N.","family":"Aziz","sequence":"additional","affiliation":[{"name":"Molecular Imaging Program, Department of Radiology, School of Medicine, Stanford University, Stanford, CA 94305, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiying","family":"Wang","sequence":"additional","affiliation":[{"name":"Bristol Myers Squibb, Princeton, NJ 08540, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leticia","family":"Brotto","sequence":"additional","affiliation":[{"name":"Bone-Muscle Research Center, College of Nursing and Health Innovation, The University of Texas at Arlington, Arlington, TX 76019, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung Eun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Surgery, School of Medicine, University of Virginia, Charlottesville, VA 22903, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jongsoo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Surgery, School of Medicine, University of Virginia, Charlottesville, VA 22903, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajendiran","family":"Karthikraj","sequence":"additional","affiliation":[{"name":"Bone-Muscle Research Center, College of Nursing and Health Innovation, The University of Texas at Arlington, Arlington, TX 76019, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5639-442X","authenticated-orcid":false,"given":"Liubov V.","family":"Gushchina","sequence":"additional","affiliation":[{"name":"Jerry R. Mendell Center for Gene Therapy, Abigail Wexner Research Institute at Nationwide Children\u2019s Hospital, Columbus, OH 43205, USA"},{"name":"Department of Pediatrics, The Ohio State University, Columbus, OH 43210, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noah","family":"Weisleder","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky, Lexington, KY 40536, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Brotto","sequence":"additional","affiliation":[{"name":"Bone-Muscle Research Center, College of Nursing and Health Innovation, The University of Texas at Arlington, Arlington, TX 76019, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1016\/j.cell.2013.05.039","article-title":"The hallmarks of aging","volume":"153","author":"Blasco","year":"2013","journal-title":"Cell"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Schoufour, J.D., Tieland, M., Barazzoni, R., Ben Allouch, S., Bie, J.V.D., Boirie, Y., Cruz-Jentoft, A.J., Eglseer, D., Topinkov\u00e1, E., and Visser, B. (2021). The Relevance of Diet, Physical Activity, Exercise, and Persuasive Technology in the Prevention and Treatment of Sarcopenic Obesity in Older Adults. Front. Nutr., 8.","DOI":"10.3389\/fnut.2021.661449"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1007\/s12603-009-0201-z","article-title":"Epidemiology and consequences of sarcopenia","volume":"13","year":"2009","journal-title":"J. Nutr. Health Aging"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1038\/s43587-022-00243-7","article-title":"Longitudinal phenotypic aging metrics in the Baltimore Longitudinal Study of Aging","volume":"2","author":"Kuo","year":"2022","journal-title":"Nat. Aging"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1007\/s40520-025-02995-9","article-title":"Health outcomes of sarcopenia: A consensus report by the outcome working group of the Global Leadership Initiative in Sarcopenia (GLIS)","volume":"37","author":"Beaudart","year":"2025","journal-title":"Aging Clin. Exp. Res."},{"key":"ref_6","first-page":"11","article-title":"Human aging, muscle mass, and fiber type composition","volume":"50","author":"Lexell","year":"1995","journal-title":"J. Gerontol. A Biol. Sci. Med. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/0022-510X(88)90132-3","article-title":"What is the cause of the ageing atrophy? Total number, size and proportion of different fiber types studied in whole vastus lateralis muscle from 15- to 83-year-old men","volume":"84","author":"Lexell","year":"1988","journal-title":"J. Neurol. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1016\/j.exger.2013.02.012","article-title":"The decline in skeletal muscle mass with aging is mainly attributed to a reduction in type II muscle fiber size","volume":"48","author":"Nilwik","year":"2013","journal-title":"Exp. Gerontol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.arr.2018.07.005","article-title":"The age-related loss of skeletal muscle mass and function: Measurement and physiology of muscle fibre atrophy and muscle fibre loss in humans","volume":"47","author":"Wilkinson","year":"2018","journal-title":"Ageing Res. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"E151","DOI":"10.1152\/ajpendo.00278.2006","article-title":"Satellite cell content is specifically reduced in type II skeletal muscle fibers in the elderly","volume":"292","author":"Verdijk","year":"2007","journal-title":"Am. J. Physiol.-Endocrinol. Metab."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1038\/s41413-025-00455-8","article-title":"Research progress on sarcopenia in the musculoskeletal system","volume":"13","author":"Mao","year":"2025","journal-title":"Bone Res."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Huo, F., Liu, Q., and Liu, H. (2022). Contribution of muscle satellite cells to sarcopenia. Front. Physiol., 13.","DOI":"10.3389\/fphys.2022.892749"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1002\/jcsm.12363","article-title":"Cellular and molecular mechanisms of sarcopenia: The S100B perspective","volume":"9","author":"Riuzzi","year":"2018","journal-title":"J. Cachexia Sarcopenia Muscle"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1023\/A:1020189627325","article-title":"Molecular mechanisms and therapeutics of the deficit in specific force in ageing skeletal muscle","volume":"3","author":"Delbono","year":"2002","journal-title":"Biogerontology"},{"key":"ref_15","first-page":"87","article-title":"Store-Operated Ca2+ Entry in Muscle Physiology","volume":"1","author":"Brotto","year":"2007","journal-title":"Curr. Chem. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4815","DOI":"10.1113\/jphysiol.2008.160481","article-title":"Differential dependence of store-operated and excitation-coupled Ca2+ entry in skeletal muscle on STIM1 and Orai1","volume":"586","author":"Lyfenko","year":"2008","journal-title":"J. Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3071","DOI":"10.1113\/jphysiol.2009.171876","article-title":"Minor sarcoplasmic reticulum membrane components that modulate excitation-contraction coupling in striated muscles","volume":"587","author":"Treves","year":"2009","journal-title":"J. Physiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1111\/j.1748-1716.2010.02191.x","article-title":"Ageing, but not yet senescent, rats exhibit reduced muscle quality and sarcoplasmic reticulum function","volume":"201","author":"Russ","year":"2011","journal-title":"Acta Physiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1152\/japplphysiol.01186.2004","article-title":"In vivo physiological cross-sectional area and specific force are reduced in the gastrocnemius of elderly men","volume":"99","author":"Morse","year":"2005","journal-title":"J. Appl. Physiol."},{"key":"ref_20","first-page":"271","article-title":"Functional consequences of sarcopenia and dynapenia in the elderly","volume":"13","author":"Clark","year":"2010","journal-title":"Curr. Opin. Clin. Nutr. Metab. Care"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"69","DOI":"10.5483\/BMBRep.2014.47.2.015","article-title":"Store-operated Ca2+ entry in muscle physiology and diseases","volume":"47","author":"Pan","year":"2014","journal-title":"BMB Rep."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e202213115","DOI":"10.1085\/jgp.202213115","article-title":"Mutations in proteins involved in E-C coupling and SOCE and congenital myopathies","volume":"154","author":"Rossi","year":"2022","journal-title":"J. Gen. Physiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"C187","DOI":"10.1152\/ajpcell.00008.2002","article-title":"Force generation, but not myosin ATPase activity, declines with age in rat muscle fibers","volume":"283","author":"Lowe","year":"2002","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1006\/abbi.1999.1444","article-title":"Localization of mitsugumin 29 to transverse tubules in rabbit skeletal muscle","volume":"371","author":"Brandt","year":"1999","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1042\/bj3310317","article-title":"Mitsugumin29, a novel synaptophysin family member from the triad junction in skeletal muscle","volume":"331","author":"Takeshima","year":"1998","journal-title":"Biochem. J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/S0968-0004(02)02229-6","article-title":"MARVEL: A conserved domain involved in membrane apposition events","volume":"27","author":"Valencia","year":"2002","journal-title":"Trends Biochem. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1083\/jcb.147.7.1473","article-title":"Abnormal features in skeletal muscle from mice lacking mitsugumin29","volume":"147","author":"Nishi","year":"1999","journal-title":"J. Cell Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1152\/physrev.00061.2017","article-title":"Sarcopenia: Aging-Related Loss of Muscle Mass and Function","volume":"99","author":"Larsson","year":"2019","journal-title":"Physiol. Rev."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.ijcard.2017.03.152","article-title":"Models of sarcopenia: Short review","volume":"238","author":"Palus","year":"2017","journal-title":"Int. J. Cardiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"6278","DOI":"10.1093\/hmg\/ddv334","article-title":"Muscle weakness in TPM3-myopathy is due to reduced Ca2+-sensitivity and impaired acto-myosin cross-bridge cycling in slow fibres","volume":"24","author":"Yuen","year":"2015","journal-title":"Hum. Mol. Genet."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1111\/j.1474-9726.2008.00408.x","article-title":"Compromised store-operated Ca2+ entry in aged skeletal muscle","volume":"7","author":"Zhao","year":"2008","journal-title":"Aging Cell"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"621","DOI":"10.18632\/aging.100335","article-title":"Store-operated Ca2+ entry (SOCE) contributes to normal skeletal muscle contractility in young but not in aged skeletal muscle","volume":"3","author":"Thornton","year":"2011","journal-title":"Aging"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1083\/jcb.200604166","article-title":"Muscle aging is associated with compromised Ca2+ spark signaling and segregated intracellular Ca2+ release","volume":"174","author":"Weisleder","year":"2006","journal-title":"J. Cell Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.str.2007.04.011","article-title":"Structure of synaptophysin: A hexameric MARVEL-domain channel protein","volume":"15","author":"Arthur","year":"2007","journal-title":"Structure"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1006\/abbi.2000.2148","article-title":"The role of mitsugumin 29 in transverse tubules of rabbit skeletal muscle","volume":"385","author":"Brandt","year":"2001","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1152\/physiolgenomics.00020.2005","article-title":"Enhanced resistance to fatigue and altered calcium handling properties of sarcalumenin knockout mice","volume":"23","author":"Zhao","year":"2005","journal-title":"Physiol. Genom."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1113\/jphysiol.2007.135046","article-title":"Store-operated Ca2+ entry during intracellular Ca2+ release in mammalian skeletal muscle","volume":"583","author":"Launikonis","year":"2007","journal-title":"J. Physiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.bbrc.2011.10.133","article-title":"Store-operated calcium entry is present in HL-1 cardiomyocytes and contributes to resting calcium","volume":"416","author":"Touchberry","year":"2011","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Zhao, X., Moloughney, J.G., Zhang, S., Komazaki, S., and Weisleder, N. (2012). Orai1 mediates exacerbated Ca2+ entry in dystrophic skeletal muscle. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0049862"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2121","DOI":"10.1194\/jlr.M600182-JLR200","article-title":"Agrin elicits membrane lipid condensation at sites of acetylcholine receptor clusters in C2C12 myotubes","volume":"47","author":"Gaus","year":"2006","journal-title":"J. Lipid Res."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Loy, R.E., Lueck, J.D., Mostajo-Radji, M.A., Carrell, E.M., and Dirksen, R.T. (2012). Allele-Specific Gene Silencing in Two Mouse Models of Autosomal Dominant Skeletal Myopathy. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0049757"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1089\/ten.tec.2023.0012","article-title":"Advanced Methodology for Rapid Isolation of Single Myofibers from Flexor Digitorum Brevis Muscle","volume":"29","author":"Awad","year":"2023","journal-title":"Tissue Eng. Part C Methods"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3440","DOI":"10.1016\/S0021-9258(19)83641-4","article-title":"A new generation of Ca2+ indicators with greatly improved fluorescence properties","volume":"260","author":"Grynkiewicz","year":"1985","journal-title":"J. Biol. Chem."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Mo, C., Wang, Z., Bonewald, L., and Brotto, M. (2019). Multi-Staged Regulation of Lipid Signaling Mediators during Myogenesis by COX-1\/2 Pathways. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20184326"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.aca.2017.07.024","article-title":"Targeted quantification of lipid mediators in skeletal muscles using restricted access media-based trap-and-elute liquid chromatography-mass spectrometry","volume":"984","author":"Wang","year":"2017","journal-title":"Anal. Chim. Acta"},{"key":"ref_46","first-page":"e4198","article-title":"Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles","volume":"69","author":"Park","year":"2012","journal-title":"J. Vis. Exp."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"308","DOI":"10.18632\/aging.204390","article-title":"Body weight influences musculoskeletal adaptation to long-term voluntary wheel running during aging in female mice","volume":"15","author":"Kitase","year":"2022","journal-title":"Aging"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1152\/japplphysiol.00299.2006","article-title":"Aging, muscle fiber type, and contractile function in sprint-trained athletes","volume":"101","author":"Korhonen","year":"2006","journal-title":"J. Appl. Physiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"R296","DOI":"10.1152\/ajpregu.00073.2020","article-title":"Age-related changes in isolated mouse skeletal muscle function are dependent on sex, muscle, and contractility mode","volume":"319","author":"Hill","year":"2020","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Jannas-Vela, S., Espinosa, A., Candia, A.A., Flores-Opazo, M., Pe\u00f1ailillo, L., and Valenzuela, R. (2023). The Role of Omega-3 Polyunsaturated Fatty Acids and Their Lipid Mediators on Skeletal Muscle Regeneration: A Narrative Review. Nutrients, 15.","DOI":"10.3390\/nu15040871"},{"key":"ref_51","first-page":"158936","article-title":"Polyunsaturated fatty acids, specialized pro-resolving mediators, and targeting inflammation resolution in the age of precision nutrition","volume":"1866","author":"Buddenbaum","year":"2021","journal-title":"Biochim. Biophys. Acta (BBA) Mol. Cell Biol. Lipids"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/B978-0-12-386487-1.00017-1","article-title":"Analysis of cholesterol trafficking with fluorescent probes","volume":"108","author":"Maxfield","year":"2012","journal-title":"Methods Cell Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/j.ceca.2009.02.009","article-title":"Lipid rafts\/caveolae as microdomains of calcium signaling","volume":"45","author":"Pani","year":"2009","journal-title":"Cell Calcium"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Dantsker, A.R. (2023). Roles of Cholesterol and PtdIns(4,5)P2 in the Regulation of STIM1\u2013Orai1 Channel Function. Cholesterol and PI(4,5)P2 in Vital Biological Functions: From Coexistence to Crosstalk, Springer International Publishing.","DOI":"10.1007\/978-3-031-21547-6"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2805","DOI":"10.1038\/ncomms3805","article-title":"Orai1-dependent calcium entry promotes skeletal muscle growth and limits fatigue","volume":"4","author":"Carrell","year":"2013","journal-title":"Nat. Commun."}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/16\/7\/988\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T03:04:57Z","timestamp":1783998297000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/16\/7\/988"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,4]]},"references-count":55,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["biom16070988"],"URL":"https:\/\/doi.org\/10.3390\/biom16070988","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,4]]}}}