{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T05:04:47Z","timestamp":1784091887795,"version":"3.55.0"},"reference-count":50,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T00:00:00Z","timestamp":1783900800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Bio-based Industries Joint Undertaking","award":["101023225"],"award-info":[{"award-number":["101023225"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Polyphenols are natural compounds with antioxidant properties that help prevent chronic diseases. Red onion (Allium cepa) peels are an underutilized source of polyphenols, offering a sustainable opportunity for the valorization of agricultural by-products. Polyphenol-rich extracts were obtained from red onion peels using subcritical water extraction and adsorption resin chromatography. Their biological effects were evaluated in Caenorhabditis elegans under oxidative stress conditions. A multi-omics approach integrating transcriptomics, metabolomics, and lipidomics was applied to assess molecular responses. The extract exhibited a high total phenolic content (&gt;400 mg GAE\/g) and strong antioxidant capacity, supporting a highly enriched polyphenolic profile. Survival assays confirmed the absence of toxicity at 100 \u00b5g\/mL GAE. Transcriptomic analysis revealed 158 differentially expressed genes associated with stress response and metabolic regulation. Metabolomic profiling indicated a systematic reduction in amino acid levels alongside an increase in betaine in treated worms, suggesting adaptive metabolic reprogramming. Lipidomic analysis revealed significant lipid remodeling, characterized by decreased triglycerides, increased diacylglycerols, and changes in membrane phospholipids, indicating alterations in membrane composition and cellular responses associated with oxidative stress adaptation. Together, these results support a model in which red onion peel extracts induce coordinated transcriptional and metabolic adaptations associated with cellular resilience and stress adaptation. This study highlights the potential of agro-industrial by-products as functional bioactive ingredients and demonstrates the value of multi-omics approaches for uncovering system-level responses.<\/jats:p>","DOI":"10.3390\/biom16071024","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:55:21Z","timestamp":1783990521000},"page":"1024","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Red Onion Peel Extracts on Oxidative Stress in Caenorhabditis elegans"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8628-0765","authenticated-orcid":false,"given":"H\u00e9ctor","family":"Palacios","sequence":"first","affiliation":[{"name":"Eurecat, Centre Tecnol\u00f2gic de Catalunya, Center for Omic Sciences (COS), Joint Unit Universitat Rovira i Virgili-EURECAT, Unique Scientific and Technical Infrastructures (ICTS), 43204 Reus, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9568-9821","authenticated-orcid":false,"given":"Miguel \u00c1ngel","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Eurecat, Centre Tecnol\u00f2gic de Catalunya, Center for Omic Sciences (COS), Joint Unit Universitat Rovira i Virgili-EURECAT, Unique Scientific and Technical Infrastructures (ICTS), 43204 Reus, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7287-5437","authenticated-orcid":false,"given":"Nerea","family":"Abasolo","sequence":"additional","affiliation":[{"name":"Eurecat, Centre Tecnol\u00f2gic de Catalunya, Center for Omic Sciences (COS), Joint Unit Universitat Rovira i Virgili-EURECAT, Unique Scientific and Technical Infrastructures (ICTS), 43204 Reus, 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Singh, J., Acharya, P., Jayaprakasha, G.K., and Patil, B.S. (2020). Comparative Metabolomics Profiling of Polyphenols, Nutrients and Antioxidant Activities of Two Red Onion (Allium cepa L.) Cultivars. Plants, 9.","DOI":"10.3390\/plants9091077"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Teichenn\u00e9, J., Tobajas, Y., Leonard, K., Tchoumtchoua, J., and Escot\u00e9, X. (2025). Sustainable Polyphenol-Rich Extracts from Agricultural By-Products: Infectivity Inhibition Potential for Human Coronavirus 229E. Molecules, 30.","DOI":"10.3390\/molecules30081806"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.tifs.2019.11.018","article-title":"Recent Advances in the Extraction of Bioactive Compounds with Subcritical Water: A Review","volume":"95","author":"Zhang","year":"2020","journal-title":"Trends Food Sci. Technol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.foodres.2011.09.027","article-title":"Techniques to Extract Bioactive Compounds from Food By-Products of Plant Origin","volume":"46","author":"Wijngaard","year":"2012","journal-title":"Food Res. Int."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.1021\/acs.jafc.7b05700","article-title":"Use of Caenorhabditis elegans to Study the Potential Bioactivity of Natural Compounds","volume":"66","author":"Liao","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/S0076-6879(99)99017-1","article-title":"Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin\u2013Ciocalteu Reagent","volume":"299","author":"Singleton","year":"1999","journal-title":"Methods Enzymol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Ayuda-Dur\u00e1n, B., Gonz\u00e1lez-Manzano, S., Gil-S\u00e1nchez, I., Moreno-Arribas, M.V., Bartolom\u00e9, B., Sanz-Buenhombre, M., Guadarrama, A., Santos-Buelga, C., and Gonz\u00e1lez-Param\u00e1s, A.M. (2019). Antioxidant Characterization and Biological Effects of Grape Pomace Extracts Supplementation in Caenorhabditis elegans. Foods, 8.","DOI":"10.3390\/foods8020075"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated Estimation of Fold Change and Dispersion for RNA-Seq Data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Rohart, F., Gautier, B., Singh, A., and L\u00ea Cao, K.A. (2017). mixOmics: An R Package for \u2018Omics Feature Selection and Multiple Data Integration. PLoS Comput. Biol., 13.","DOI":"10.1101\/108597"},{"key":"ref_11","first-page":"e62001","article-title":"CRISPR\/Cas9 Editing of the C. elegans rbm-3.2 Gene Using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes","volume":"166","author":"Smith","year":"2020","journal-title":"J. Vis. Exp."},{"key":"ref_12","first-page":"C1533","article-title":"Cloning and Functional Characterization of a Folate Transporter from the Nematode Caenorhabditis elegans","volume":"292","author":"Balamurugan","year":"2007","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e48119","DOI":"10.7554\/eLife.48119","article-title":"Evolution of Yin and Yang Isoforms of a Chromatin Remodeling Subunit Precedes the Creation of Two Genes","volume":"8","author":"Long","year":"2019","journal-title":"eLife"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Crooks, B.A., McKenzie, D., Cadd, L.C., McCoy, C.J., McVeigh, P., Marks, N.J., Maule, A.G., Mousley, A., and Atkinson, L.E. (2022). Pan-Phylum In Silico Analyses of Nematode Endocannabinoid Signalling Systems Highlight Novel Opportunities for Parasite Drug Target Discovery. Front. Endocrinol., 13.","DOI":"10.3389\/fendo.2022.892758"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1534\/genetics.118.301518","article-title":"Multiple Histone Methyl-Lysine Readers Ensure Robust Development and Germline Immortality in Caenorhabditis elegans","volume":"210","author":"Saltzman","year":"2018","journal-title":"Genetics"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Liu, Y.J., Janssens, G.E., McIntyre, R.L., Molenaars, M., Kamble, R., Gao, A.W., Jongejan, A., van Weeghel, M., MacInnes, A.W., and Houtkooper, R.H. (2019). Glycine Promotes Longevity in Caenorhabditis elegans in a Methionine Cycle-Dependent Fashion. PLoS Genet., 15.","DOI":"10.1371\/journal.pgen.1007633"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.freeradbiomed.2018.09.035","article-title":"Lonicera japonica Extends Lifespan and Healthspan in Caenorhabditis elegans","volume":"129","author":"Yang","year":"2018","journal-title":"Free Radic. Biol. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"105869","DOI":"10.1016\/j.jff.2023.105869","article-title":"The Positive Effects of Camellia oleifera Oil on Lifespan in Caenorhabditis elegans","volume":"110","author":"Liu","year":"2023","journal-title":"J. Funct. Foods"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Hao, L., Johnsen, R., Lauter, G., Baillie, D., and B\u00fcrglin, T.R. (2006). Comprehensive Analysis of Gene Expression Patterns of Hedgehog-Related Genes. BMC Genom., 7.","DOI":"10.1186\/1471-2164-7-280"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"173","DOI":"10.3164\/jcbn.23-44","article-title":"The Role of Nutrition and Oxidative Stress as Aging Factors in Caenorhabditis elegans","volume":"73","author":"Yasuda","year":"2023","journal-title":"J. Clin. Biochem. Nutr."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.1534\/g3.118.200056","article-title":"Effects of Larval Density on Gene Regulation in Routine L1 Synchronization of C. elegans","volume":"8","author":"Chang","year":"2018","journal-title":"G3"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1007\/s13206-011-5112-5","article-title":"Transcriptomic Analysis of Caenorhabditis elegans Exposed to Nickel(II) Acetate Using Microarray","volume":"5","author":"Jeong","year":"2011","journal-title":"BioChip J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1101\/gr.184401","article-title":"Changes in Gene Expression Associated with Developmental Arrest and Longevity in Caenorhabditis elegans","volume":"11","author":"Jones","year":"2001","journal-title":"Genome Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1093\/genetics\/155.3.1139","article-title":"dpy-18 Encodes an \u03b1-Subunit of Prolyl-4-Hydroxylase in Caenorhabditis elegans","volume":"155","author":"Hill","year":"2000","journal-title":"Genetics"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.cell.2022.11.001","article-title":"Hallmarks of Aging: An Expanding Universe","volume":"186","author":"Blasco","year":"2023","journal-title":"Cell"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Edwards, C., Canfield, J., Copes, N., Brito, A., Rehan, M., Lipps, D., Brunquell, J., Westerheide, S.D., and Bradshaw, P.C. (2015). Mechanisms of Amino Acid-Mediated Lifespan Extension in Caenorhabditis elegans. BMC Genet., 16.","DOI":"10.1186\/s12863-015-0167-2"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.freeradbiomed.2017.01.009","article-title":"High Concentration of Branched-Chain Amino Acids Promotes Oxidative Stress, Inflammation and Migration of Human Peripheral Blood Mononuclear Cells via mTORC1 Activation","volume":"104","author":"Zhenyukh","year":"2017","journal-title":"Free Radic. Biol. Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1093\/gerona\/glae221","article-title":"Senolytics Enhance the Longevity of Caenorhabditis elegans by Altering Betaine Metabolism","volume":"79","author":"Lan","year":"2024","journal-title":"J. Gerontol. A Biol. Sci. Med. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Ho, N.S.H., and Taubert, S. (2012). Function and Regulation of Lipid Biology in Caenorhabditis elegans Aging. Front. Physiol., 3.","DOI":"10.3389\/fphys.2012.00143"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.abb.2015.06.003","article-title":"The Caenorhabditis elegans Lipidome: A Primer for Lipid Analysis in Caenorhabditis elegans","volume":"589","author":"Witting","year":"2016","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2077","DOI":"10.1021\/jf104217g","article-title":"Use of Saccharomyces cerevisiae and Caenorhabditis elegans as Model Organisms to Study the Effect of Cocoa Polyphenols in the Resistance to Oxidative Stress","volume":"59","author":"Martorell","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"463","DOI":"10.3945\/ajcn.113.073445","article-title":"Diets Naturally Rich in Polyphenols Improve Fasting and Postprandial Dyslipidemia and Reduce Oxidative Stress: A Randomized Controlled Trial","volume":"99","author":"Annuzzi","year":"2014","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Feldman, F., Koudoufio, M., Desjardins, Y., Spahis, S., Delvin, E., and Levy, E. (2021). Efficacy of Polyphenols in the Management of Dyslipidemia: A Focus on Clinical Studies. Nutrients, 13.","DOI":"10.3390\/nu13020672"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e14061","DOI":"10.1111\/jfbc.14061","article-title":"High-Intensity Interval Training along with Spirulina Algae Consumption and Caloric Restriction Ameliorated the Nrf1\/Tfam\/Mgmt and ATP5A1 Pathway in the Heart Tissue of Obese Rats","volume":"46","author":"Yousefian","year":"2022","journal-title":"J. Food Biochem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"5768953","DOI":"10.1155\/2019\/5768953","article-title":"The Effects of Age and Reproduction on the Lipidome of Caenorhabditis elegans","volume":"2019","author":"Wan","year":"2019","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1007\/s10522-013-9450-7","article-title":"Lipidomics in Longevity and Healthy Aging","volume":"14","year":"2013","journal-title":"Biogerontology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"650","DOI":"10.18632\/aging.204515","article-title":"The Lipidomes of C. elegans with Mutations in asm-3 and hyl-2 Show Distinct Lipid Profiles during Aging","volume":"15","author":"Staab","year":"2023","journal-title":"Aging"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Perez, M.A., and Watts, J.L. (2021). Worms, Fat, and Death: Caenorhabditis elegans Lipid Metabolites Regulate Cell Death. Metabolites, 11.","DOI":"10.3390\/metabo11020125"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.mad.2014.11.002","article-title":"Sphingolipid Metabolism Regulates Development and Lifespan in Caenorhabditis elegans","volume":"143\u2013144","author":"Cutler","year":"2014","journal-title":"Mech. Ageing Dev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"103734","DOI":"10.1016\/j.isci.2022.103734","article-title":"Multi-Omics Analysis Identifies Essential Regulators of Mitochondrial Stress Response in Two Wild-Type C. elegans Strains","volume":"25","author":"Gao","year":"2022","journal-title":"iScience"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Dai, Y., Tang, H., and Pang, S. (2021). The Crucial Roles of Phospholipids in Aging and Lifespan Regulation. Front. Physiol., 12.","DOI":"10.3389\/fphys.2021.775648"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1002\/biof.1755","article-title":"Antioxidant and Reducing Lipid Accumulation Effects of Rutin in Caenorhabditis elegans","volume":"47","author":"Qin","year":"2021","journal-title":"Biofactors"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3589","DOI":"10.1038\/s41467-026-71508-7","article-title":"Aging-Associated Decline of Phosphatidylcholine Synthesis Is a Malleable Trigger of Natural Mitochondrial Aging","volume":"17","author":"Poliezhaieva","year":"2026","journal-title":"Nat. Commun."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"111498","DOI":"10.1016\/j.mad.2021.111498","article-title":"Supplementation with Phosphatidylethanolamine Confers Antioxidant and Anti-Aging Effects via Hormesis and Reduced Insulin\/IGF-1-Like Signaling in C. elegans","volume":"197","author":"Park","year":"2021","journal-title":"Mech. Ageing Dev."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/s10522-020-09856-0","article-title":"Phosphatidylserine Modulates Response to Oxidative Stress through Hormesis and Increases Lifespan via DAF-16 in Caenorhabditis elegans","volume":"21","author":"Kim","year":"2020","journal-title":"Biogerontology"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"763","DOI":"10.14348\/molcells.2022.0097","article-title":"Recent Progress in Regulation of Aging by Insulin\/IGF-1 Signaling in Caenorhabditis elegans","volume":"45","author":"Lee","year":"2022","journal-title":"Mol. Cells"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2529","DOI":"10.1101\/gad.1218504","article-title":"Identification of C. elegans DAF-12 Binding Sites, Response Elements, and Target Genes","volume":"18","author":"Shostak","year":"2004","journal-title":"Genes Dev."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.mod.2004.02.001","article-title":"The C. elegans Eukaryotic Initiation Factor 5A Homologue, IFF-1, Is Required for Germ Cell Proliferation, Gametogenesis and Localization of the P-Granule Component PGL-1","volume":"121","author":"Hanazawa","year":"2004","journal-title":"Mech. Dev."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1093\/jb\/mvt074","article-title":"Galectin LEC-1 Plays a Defensive Role against Oxidative Stress in Caenorhabditis elegans","volume":"154","author":"Takeuchi","year":"2013","journal-title":"J. Biochem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"20150299","DOI":"10.1098\/rstb.2015.0299","article-title":"Antimicrobial Effectors in the Nematode Caenorhabditis elegans: An Outgroup to the Arthropoda","volume":"371","author":"Dierking","year":"2016","journal-title":"Philos. Trans. R. Soc. 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