{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T09:58:41Z","timestamp":1785232721275,"version":"3.55.0"},"reference-count":52,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Background: Childhood obesity is a growing health concern and added sugar intake has been associated with increased obesity risk. Emerging evidence suggests that miR-143-5p and miR-223-3p are involved in metabolic regulation. Therefore, this study evaluated their plasma expression according to added sugar intake and their association with cardiometabolic risk parameters. Methods: This cross-sectional study included 91 children aged 6\u201312 years, comprising 44 with normal weight and 47 with overweight\/obesity. Anthropometric measurements, clinical characteristics, and dietary added sugar intake were assessed. Plasma expression of miR-143-5p and miR-223-3p was quantified by RT-qPCR. Results: Compared with low added sugar intake, high added sugar intake was associated with lower plasma miR-143-5p expression and higher miR-223-3p expression. Both miR-143-5p and miR-223-3p expression levels were higher in children with overweight\/obesity and insulin resistance than in their respective counterparts. In adjusted analyses, neither miRNA was associated with cardiometabolic risk parameters. However, added sugar intake was positively associated with miR-223-3p expression (\u03b2 = 0.188, 95% CI: 0.022, 0.354) and glucose levels (\u03b2 = 1.944, 95% CI: 0.133, 3.755). Conclusions: These findings suggest that higher added sugar intake is associated with altered circulating miRNA expression and higher fasting glucose concentrations in school-aged children.<\/jats:p>","DOI":"10.3390\/biom16081100","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T08:52:01Z","timestamp":1785228721000},"page":"1100","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Added Sugar Intake Is Associated with Altered Plasma miR-143-5p and miR-223-3p Expression and Fasting Glucose Concentrations in Children from Mexico City"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-3859-3532","authenticated-orcid":false,"given":"Alejandro","family":"Casta\u00f1eda-L\u00f3pez","sequence":"first","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Bioqu\u00edmica, Hospital de Especialidades, Centro M\u00e9dico Nacional Siglo XXI, Instituto Mexicano del Seguro Social (IMSS), Mexico City 06720, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda F.","family":"Vel\u00e1zquez-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias de La Salud, Universidad Tecnol\u00f3gica de M\u00e9xico (UNITEC) Campus Los Reyes, Los Reyes 56400, State of Mexico, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5129-8108","authenticated-orcid":false,"given":"Leonor","family":"Jacobo-Albavera","sequence":"additional","affiliation":[{"name":"Laboratorio de Gen\u00f3mica de Enfermedades Cardiovasculares, Instituto Nacional de Medicina Gen\u00f3mica (INMEGEN), Mexico City 14610, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Fernanda","family":"P\u00e9rez-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Bioqu\u00edmica, Hospital de Especialidades, Centro M\u00e9dico Nacional Siglo XXI, Instituto Mexicano del Seguro Social (IMSS), Mexico City 06720, Mexico"},{"name":"Programa de Doctorado en Ciencias Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), Mexico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 de Jes\u00fas","family":"Peralta-Romero","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Bioqu\u00edmica, Hospital de Especialidades, Centro M\u00e9dico Nacional Siglo XXI, Instituto Mexicano del Seguro Social (IMSS), Mexico City 06720, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5771-8780","authenticated-orcid":false,"given":"Sof\u00eda","family":"Barrag\u00e1n-V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Nutritional Epidemiology Group, School of Food Science & Nutrition, University of Leeds, Woodhouse Ln, Woodhouse, Leeds LS2 9\u2009JT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5863-1169","authenticated-orcid":false,"given":"Claudia I.","family":"Ram\u00edrez-Silva","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Salud P\u00fablica, Cuernavaca 62100, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s","family":"Rocha-Aguado","sequence":"additional","affiliation":[{"name":"OOAD DF Norte del Instituto Mexicano del Seguro Social, Unidad de Medicina Familiar No. 23, Mexico City 07070, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6503-694X","authenticated-orcid":false,"given":"Fengyang","family":"Huang","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Obesidad y Asma, Hospital Infantil de M\u00e9xico Federico G\u00f3mez, Mexico City 06720, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fausto","family":"S\u00e1nchez-Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Mexico City 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Cruz","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Bioqu\u00edmica, Hospital de Especialidades, Centro M\u00e9dico Nacional Siglo XXI, Instituto Mexicano del Seguro Social (IMSS), Mexico City 06720, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6569-2851","authenticated-orcid":false,"given":"Adri\u00e1n","family":"Hern\u00e1ndez-D\u00edazcouder","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Bioqu\u00edmica, Hospital de Especialidades, Centro M\u00e9dico Nacional Siglo XXI, 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