{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T05:27:10Z","timestamp":1786512430311,"version":"build-2736575974"},"reference-count":27,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science Fund for Distinguished Young Scholars of Shaanxi Province","award":["2024JC-JCQN-30"],"award-info":[{"award-number":["2024JC-JCQN-30"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32372852"],"award-info":[{"award-number":["32372852"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"award":["32372852"],"award-info":[{"award-number":["32372852"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2022YFF1000100"],"award-info":[{"award-number":["2022YFF1000100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Shaanxi Provincial Innovation Leadership Program in Sciences and Technologies for Young and Middle-Aged Scientists","award":["2023SR205"],"award-info":[{"award-number":["2023SR205"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>The genetic and physiological variations among individuals of Holstein dairy cattle contribute to variations in milk yield. Selecting high-yield cows is critical for improving lactation performance. The present study aimed to identify functional genetic variants of the 3\u2032 untranslated region (3\u2032 UTR) in the ELF5 gene associated with milk production traits and to elucidate their underlying molecular mechanisms. A total of 1314 Chinese Holstein cows were genotyped, and a novel single nucleotide polymorphism (SNP), ELF5 g.32793 G&gt;T, was identified in the 3\u2032 UTR of ELF5. This SNP was significantly associated with milk production, such as milk yield, milk fat percentage, milk protein percentage, and somatic cell score. Bioinformatic prediction and dual-luciferase reporter assays demonstrated that the ELF5 g.32793 G&gt;T affects the interaction between bta-miR-487a and the ELF5 3\u2032 UTR. Functional analyses in bovine mammary epithelial (MAC-T) cells showed that bta-miR-487a suppressed cell proliferation by inducing G0\/G1 phase arrest and reduced the expression of proliferation-related proteins. Collectively, these findings suggested that bta-miR-487a participates in the regulation of MAC-T cell growth and that the ELF5 g.32793 G&gt;T polymorphism may influence lactation performance through an miRNA-associated mechanism. Therefore, the variant can be used as a functional marker for marker-assisted selection in dairy cattle breeding.<\/jats:p>","DOI":"10.3390\/biom16081163","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T08:09:36Z","timestamp":1786435776000},"page":"1163","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Functional SNP Variant of the 3\u2032 UTR Within the ELF5 Gene Affects Lactation Traits Through bta-miR-487a-Mediated Regulation in Holstein Dairy Cattle"],"prefix":"10.3390","volume":"16","author":[{"given":"Lingyuan","family":"Ma","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haotian","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanying","family":"Pan","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0323-254X","authenticated-orcid":false,"given":"Mingxun","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2254-5805","authenticated-orcid":false,"given":"Xianyong","family":"Lan","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2357-8094","authenticated-orcid":false,"given":"Yang","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Bai, Y., Han, K., Xiong, L., Li, Y., Liao, R., and Wang, F. 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