{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T11:28:59Z","timestamp":1787225339938,"version":"build-2736575974"},"reference-count":102,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Committee of Science of the Ministry of Science and Higher Education of the Republic of Kazakhstan","award":["BR24992841"],"award-info":[{"award-number":["BR24992841"]}]},{"name":"Committee of Science of the Ministry of Science and Higher Education of the Republic of Kazakhstan","award":["AP23485899"],"award-info":[{"award-number":["AP23485899"]}]},{"award":["BR24992841"],"award-info":[{"award-number":["BR24992841"]}],"id":[{"id":"https:\/\/ror.org\/05tyne317","id-type":"ROR","asserted-by":"publisher"}]},{"award":["AP23485899"],"award-info":[{"award-number":["AP23485899"]}],"id":[{"id":"https:\/\/ror.org\/05tyne317","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Nazarbayev University CRP","award":["111024CRP2009"],"award-info":[{"award-number":["111024CRP2009"]}]},{"award":["111024CRP2009"],"award-info":[{"award-number":["111024CRP2009"]}],"id":[{"id":"https:\/\/ror.org\/052bx8q98","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"French National Research Agency","doi-asserted-by":"publisher","award":["ANR-22-CE12-0034-01"],"award-info":[{"award-number":["ANR-22-CE12-0034-01"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"award":["ANR-22-CE12-0034-01"],"award-info":[{"award-number":["ANR-22-CE12-0034-01"]}],"id":[{"id":"https:\/\/ror.org\/00rbzpz17","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Russian Ministry of Higher Education and Science","award":["FSUS-2025-0012"],"award-info":[{"award-number":["FSUS-2025-0012"]}]},{"name":"National Cancer Institute of the National Institutes of Health","award":["CA067985"],"award-info":[{"award-number":["CA067985"]}]},{"name":"Franco-Kazakhstan fellowship Abai-Vern","award":["159826Y"],"award-info":[{"award-number":["159826Y"]}]},{"name":"Kazakhstan Bolashaq International Scholarship","award":["15-1-15\/4652"],"award-info":[{"award-number":["15-1-15\/4652"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>The Escherichia coli adenine-DNA glycosylase (MutY) and its human homologue, MUTYH, protect cells against oxygen-free radical-induced mutagenesis by excising regular adenine impaired with 8-oxo-7,8-dihydro-guanine (8oxoG) in the base excision repair (BER) pathway. However, removal of adenine by MutY and MUTYH from an A\u00b78oxoG pair generated via misincorporation of an oxidized nucleotide during DNA synthesis might induce A\u00b7T\u2192C\u00b7G transversions. Here, to examine MutY and MUTYH in vitro activities, we used short synthetic DNA duplexes in which the target adenine residue was positioned opposite a variety of DNA base modifications. MUTYH does not excise mismatched adenine in non-canonical DNA substrates, whereas MutY excises adenine mispaired with 1,3-d(GpNpG) cisplatin intra-strand crosslink. In addition, we characterized four MUTYH variants associated with cancer risk, which exhibit the following order of DNA glycosylase deficiency: WT \u2265 G169D &gt; G202E \u2248 Y165C &gt;&gt; D222N. Human adenine-DNA glycosylase MUTYH and its mutant variants, contrary to bacterial MutY, are not prone to aberrant removal of regular adenine residues opposite modified residues in DNA duplexes. We hypothesize that E. coli MutY is prone to aberrant repair under certain conditions and that this may prevent incorporation of adenine opposite blocking lesions in the template strand.<\/jats:p>","DOI":"10.3390\/biom16081214","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T10:55:18Z","timestamp":1787223318000},"page":"1214","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["DNA Damage Recognition by Bacterial and Human Adenine-DNA Glycosylases: Insights from Non-Canonical Substrates"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-9292-0153","authenticated-orcid":false,"given":"Ulan","family":"Sarsenbayeva","sequence":"first","affiliation":[{"name":"Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan"},{"name":"Department of Biochemistry, Asfendiyarov Kazakh National Medical University, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7984-6177","authenticated-orcid":false,"given":"Didier","family":"Gasparutto","sequence":"additional","affiliation":[{"name":"CEA, CNRS, Institut National Polytechnique de Grenoble, Institut de Recherche Interdisciplinaire de Grenoble, Syst\u00e8mes Mol\u00e9culaires et nanoMat\u00e9riaux pour l\u2019\u00c9nergie et la Sant\u00e9\u2014Unit\u00e9 Mixte de Recherche 5819, University Grenoble Alpes, 38000 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7961-2231","authenticated-orcid":false,"given":"Nicolas","family":"Geacintov","sequence":"additional","affiliation":[{"name":"Chemistry Department, New York University, 31 Washington Place, New York, NY 10003, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0488-8858","authenticated-orcid":false,"given":"Alexander A.","family":"Ishchenko","sequence":"additional","affiliation":[{"name":"CNRS-UMR9019 Genome Integrity and Cancers, Gustave Roussy Cancer Campus, Universit\u00e9 Paris-Saclay, 94805 Villejuif, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gulzhan","family":"Zhamanbayeva","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamalidin O.","family":"Sharipov","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Asfendiyarov Kazakh National Medical University, Almaty 050012, Kazakhstan"},{"name":"Structural and Functional Genomics Laboratory, M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty 050012, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos H.","family":"Trasvi\u00f1a-Arenas","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n sobre el Envejecimiento, Centro de Investigaci\u00f3n y de Estudios Avanzados (CINVESTAV), Unidad Sede Sur, Tlalpan, Ciudad de M\u00e9xico 14330, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5873-7935","authenticated-orcid":false,"given":"Sheila S.","family":"David","sequence":"additional","affiliation":[{"name":"Chemical Biology Graduate Program, Department of Chemistry and Chemistry, University of California, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry O.","family":"Zharkov","sequence":"additional","affiliation":[{"name":"SB RAS Institute of Chemical Biology and Fundamental Medicine, Lavrentiev Av. 8, Novosibirsk 630090, Russia"},{"name":"Department of Natural Sciences, Novosibirsk State University, 2 Pirogova St., Novosibirsk 630090, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0775-7324","authenticated-orcid":false,"given":"Bakhyt T.","family":"Matkarimov","sequence":"additional","affiliation":[{"name":"National Laboratory Astana, Nazarbayev University, Astana 010000, Kazakhstan"},{"name":"L.N. Gumilyov Eurasian National University, Astana 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4630-1074","authenticated-orcid":false,"given":"Murat","family":"Saparbaev","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan"},{"name":"CNRS-UMR9019 Genome Integrity and Cancers, Gustave Roussy Cancer Campus, Universit\u00e9 Paris-Saclay, 94805 Villejuif, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9499-1682","authenticated-orcid":false,"given":"Sabira","family":"Taipakova","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan"},{"name":"Scientific Research Institute of Biology and Biotechnology Problems, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"103051","DOI":"10.1016\/j.dnarep.2021.103051","article-title":"Cellular response to endogenous DNA damage: DNA base modifications in gene expression regulation","volume":"99","author":"Bordin","year":"2021","journal-title":"DNA Repair"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.freeradbiomed.2016.11.042","article-title":"Repair of 8-oxo-7,8-dihydroguanine in prokaryotic and eukaryotic cells: Properties and biological roles of the Fpg and OGG1 DNA N-glycosylases","volume":"107","author":"Boiteux","year":"2017","journal-title":"Free Radic. 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