{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T11:16:12Z","timestamp":1788434172356,"version":"build-2803163510"},"reference-count":88,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Greece"},{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/019w4f821","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010623","name":"University of Thessaly","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100010623","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/04v4g9h31","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>As aquafeed formulations include more plant-based materials, mycotoxin contamination is becoming relevant for fish farming. Deoxynivalenol (DON) and fumonisin B1 (FB1) frequently occur in fish feeds and may impair fish performance and health. This study evaluated the impact of DON and FB1 on growth and health parameters in gilthead seabream (Sparus aurata). Fish were fed ad libitum for seven weeks with six diets assessed in triplicate: DON A (300 ppb), DON B (2000 ppb), DON C (5000 ppb), FB1 A (5 ppb), FB1 B (10 ppb), FB1 C (40 ppb). The highest contamination levels significantly reduced feed intake, body weight, total length, and biomass, while body weight was also reduced in the lowest FB1 treatment. The feed conversion ratio increased in exposed fish, whereas the specific growth rate decreased in DON B, DON C, FB1 A, and FB1 C. Both mycotoxins altered hematological profiles, immune-related enzymatic activities, and liver morphology. DNA damage increased with exposure level and was greatest at the highest DON concentration. Overall, this study shows that short-term dietary exposure can impair seabream performance and health, supporting stricter mycotoxin monitoring in fish feeds and research on chronic exposure, recovery, and species-specific safety thresholds.<\/jats:p>","DOI":"10.3390\/biom16091258","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T01:05:26Z","timestamp":1788224726000},"page":"1258","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Deoxynivalenol and Fumonisin B1 in Gilthead Seabream Diets: Impact on Growth Performance, Hematology, Immunology, and Histopathology"],"prefix":"10.3390","volume":"16","author":[{"given":"Christina","family":"Papadouli","sequence":"first","affiliation":[{"name":"Department of Ichthyology and Aquatic Environment-Aquaculture Laboratory, School of Agricultural Sciences, University of Thessaly, Fytokou Str., 38446 Volos, 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Greece"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8911-6362","authenticated-orcid":false,"given":"Morgane","family":"Henry","sequence":"additional","affiliation":[{"name":"Institute of Marine Biology, Biotechnology, and Aquaculture, Hellenic Centre for Marine Research, 46.7 km Athens-Sounion, 19013 Attiki, 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Hellenic Centre for Marine Research, 46.7 km Athens-Sounion, 19013 Attiki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3296-9943","authenticated-orcid":false,"given":"Petros","family":"Chronopoulos","sequence":"additional","affiliation":[{"name":"Institute of Marine Biology, Biotechnology, and Aquaculture, Hellenic Centre for Marine Research, 46.7 km Athens-Sounion, 19013 Attiki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Eleni","family":"Golomazou","sequence":"additional","affiliation":[{"name":"Department of Ichthyology and Aquatic Environment-Aquaculture Laboratory, School of Agricultural Sciences, University of Thessaly, Fytokou Str., 38446 Volos, 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The State of World Fisheries and Aquaculture 2024\u2014Blue Transformation in Action, FAO."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.oneear.2019.10.018","article-title":"The future of aquatic protein: Implications for protein sources in aquaculture diets","volume":"1","author":"Hua","year":"2019","journal-title":"One Earth"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1016\/j.marpol.2010.01.027","article-title":"Where do fishmeal and fish oil products come from? An analysis of the conversion ratios in the global fishmeal industry","volume":"34","author":"Mittaine","year":"2010","journal-title":"Mar. Policy"},{"key":"ref_4","unstructured":"Index Mundi (2026, June 30). Fishmeal Monthly Price\u2014US Dollars per Metric Ton. Index Mundi Commodity Prices Database. Available online: https:\/\/www.indexmundi.com\/commodities\/?commodity=fish-meal."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1007\/s11367-020-01759-z","article-title":"Fishmeal partial substitution within aquafeed formulations: Life cycle assessment of four alternative protein sources","volume":"25","author":"Maiolo","year":"2020","journal-title":"Int. J. Life Cycle Assess."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.aquaculture.2018.06.003","article-title":"Aquaculture waste production associated with antinutrient presence in common fish feed plant ingredients","volume":"495","author":"Kokou","year":"2018","journal-title":"Aquaculture"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/j.aquaculture.2018.03.001","article-title":"Impact of deoxynivalenol on rainbow trout: Growth performance, digestibility, key gene expression regulation and metabolism","volume":"490","author":"Gilannejad","year":"2018","journal-title":"Aquaculture"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Kovalsky, P., Kos, G., N\u00e4hrer, K., Schwab, C., Jenkins, T., Schatzmayr, G., and Sulyok, M. (2016). Co-occurrence of regulated, masked and emerging mycotoxins and secondary metabolites in finished feed and maize\u2014An extensive survey. Toxins, 8.","DOI":"10.3390\/toxins8120363"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1186\/s40104-022-00714-2","article-title":"Nutritional impact of mycotoxins in food animal production and strategies for mitigation","volume":"13","author":"Xu","year":"2022","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Awuchi, C.G., Ondari, E.N., Nwozo, S., Odongo, G.A., Eseoghene, I.J., Twinomuhwezi, H., Ogbonna, C.U., Upadhyay, A.K., Adeleye, A.O., and Okpala, C.O.R. (2022). Mycotoxins\u2019 toxicological mechanisms involving humans, livestock and their associated health concerns: A review. Toxins, 14.","DOI":"10.3390\/toxins14030167"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"104095","DOI":"10.1016\/j.micpath.2020.104095","article-title":"Mycotoxin contamination and control strategy in human, domestic animal and poultry: A review","volume":"142","author":"Haque","year":"2020","journal-title":"Microb. Pathog."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Koletsi, P., Schrama, J.W., Graat, E.A.M., Wiegertjes, G.F., Lyons, P., and Pietsch, C. (2021). The occurrence of mycotoxins in raw materials and fish feeds in Europe and the potential effects of deoxynivalenol (DON) on the health and growth of farmed fish species\u2014A review. Toxins, 13.","DOI":"10.3390\/toxins13060403"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/s12550-019-00368-6","article-title":"Risk assessment for mycotoxin contamination in fish feeds in Europe","volume":"36","author":"Pietsch","year":"2020","journal-title":"Mycotoxin Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"107610","DOI":"10.1016\/j.foodcont.2020.107610","article-title":"Using metabolomics to guide strategies to tackle the issue of the contamination of food and feed with mycotoxins: A review of the literature with specific focus on Fusarium mycotoxins","volume":"121","author":"Atanasova","year":"2021","journal-title":"Food Control"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Li, Y., Gao, H., Wang, R., and Xu, Q. (2023). Deoxynivalenol in food and feed: Recent advances in decontamination strategies. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1141378"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.aquaculture.2019.02.019","article-title":"A comparative investigation of the effects of feed-borne deoxynivalenol (DON) on growth performance, nutrient utilization and metabolism of detoxification in rainbow trout (Oncorhynchus mykiss) and Nile tilapia (Oreochromis niloticus) fed diets containing different levels of digestible carbohydrates","volume":"505","author":"Hooft","year":"2019","journal-title":"Aquaculture"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.fct.2014.03.012","article-title":"In vivo effects of deoxynivalenol (DON) on innate immune responses of carp (Cyprinus carpio L.)","volume":"68","author":"Pietsch","year":"2014","journal-title":"Food Chem. Toxicol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Moldal, T., Bernhoft, A., Rosenlund, G., Kaldhusdal, M., and Koppang, E.O. (2018). Dietary deoxynivalenol (DON) may impair the epithelial barrier and modulate the cytokine signaling in the intestine of Atlantic salmon (Salmo salar). Toxins, 10.","DOI":"10.3390\/toxins10090376"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Lumsangkul, C., Chiang, H.-I., Lo, N.-W., Fan, Y.-K., and Ju, J.-C. (2019). Developmental toxicity of mycotoxin fumonisin B1 in animal embryogenesis: An overview. Toxins, 11.","DOI":"10.3390\/toxins11020114"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"EFSA Panel on Contaminants in the Food Chain (CONTAM) (2018). Risks for animal health related to the presence of fumonisins, their modified forms and hidden forms in feed. EFSA J., 16, e05242.","DOI":"10.2903\/j.efsa.2018.5242"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Oliveira, M., and Vasconcelos, V. (2020). Occurrence of mycotoxins in fish feed and its effects: A review. Toxins, 12.","DOI":"10.3390\/toxins12030160"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/S0044-8486(02)00268-5","article-title":"Responses of Nile tilapia (Oreochromis niloticus) fed diets containing different concentrations of moniliformin or fumonisin B1","volume":"217","author":"Tuan","year":"2003","journal-title":"Aquaculture"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/s12550-010-0059-2","article-title":"Growth performance, haematology and serum biochemistry of African catfish (Clarias gariepinus) fingerlings fed graded levels of dietary fumonisin B1","volume":"26","author":"Gbore","year":"2010","journal-title":"Mycotoxin Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.aninu.2024.11.023","article-title":"Mechanism of fumonisin B1 on growth performance and intestinal structural integrity of juvenile grass carp (Ctenopharyngodon idella)","volume":"21","author":"Chen","year":"2025","journal-title":"Anim. Nutr."},{"key":"ref_25","unstructured":"Federation of European Aquaculture Producers (FEAP) (2025). European Aquaculture Production Report 2017\u20132023 (V1.0), Federation of European Aquaculture Producers."},{"key":"ref_26","unstructured":"Hellenic Aquaculture Producers Organization (HAPO) (2024). Aquaculture Annual Report 2024, Hellenic Aquaculture Producers Organization. Available online: https:\/\/fishfromgreece.com\/wp-content\/uploads\/2024\/10\/HAPO_AR24_Spreads.pdf."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1111\/raq.12159","article-title":"Occurrence of mycotoxins in commercial aquafeeds in Asia and Europe: A real risk to aquaculture?","volume":"10","author":"Naehrer","year":"2018","journal-title":"Rev. Aquac."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.chemosphere.2015.02.021","article-title":"Occurrence and potential transfer of mycotoxins in gilthead sea bream and Atlantic salmon by use of novel alternative feed ingredients","volume":"128","author":"Serrano","year":"2015","journal-title":"Chemosphere"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"107537","DOI":"10.1016\/j.foodcont.2020.107537","article-title":"Natural occurrence of ochratoxin A (OTA) in edible and not edible tissue of farmed gilthead seabream (Sparus aurata) and European seabass (Dicentrarchus labrax) sold on the Italian market","volume":"120","author":"Meucci","year":"2021","journal-title":"Food Control"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1007\/s10499-010-9326-7","article-title":"Effects of additive iron on growth, tissue distribution, haematology and immunology of gilthead sea bream, Sparus aurata","volume":"18","author":"Rigos","year":"2010","journal-title":"Aquac. Int."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"101347","DOI":"10.1016\/j.aqrep.2022.101347","article-title":"Partial dietary fishmeal replacement with full-fat or defatted superworm (Zophobas morio) larvae meals modulates the innate immune system of gilthead seabream, Sparus aurata","volume":"27","author":"Henry","year":"2022","journal-title":"Aquac. Rep."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.aquaculture.2012.08.009","article-title":"Growth performance, feed utilization and non-specific immune response of gilthead sea bream (Sparus aurata L.) fed graded levels of a bioprocessed soybean meal","volume":"364\u2013365","author":"Kokou","year":"2012","journal-title":"Aquaculture"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.dci.2015.03.010","article-title":"Strong effect of long-term Sparicotyle chrysophrii infection on the cellular and innate immune responses of gilthead sea bream, Sparus aurata","volume":"51","author":"Henry","year":"2015","journal-title":"Dev. Comp. Immunol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.fsi.2014.02.005","article-title":"Optimal dietary protein\/lipid ratio for improved immune status of a newly cultivated Mediterranean fish species, the shi drum Umbrina cirrosa, L.","volume":"37","author":"Henry","year":"2014","journal-title":"Fish Shellfish Immunol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"742820","DOI":"10.1016\/j.aquaculture.2025.742820","article-title":"Nutritional enhancement of lupin meal (Lupinus albus) through fermentation with Saccharomyces cerevisiae as plant protein ingredient in aquafeeds for the European sea bass (Dicentrarchus labrax)","volume":"609","author":"Vasilaki","year":"2025","journal-title":"Aquaculture"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/0014-4827(88)90265-0","article-title":"A simple technique for quantitation of low levels of DNA damage in individual cells","volume":"175","author":"Singh","year":"1988","journal-title":"Exp. Cell Res."},{"key":"ref_37","unstructured":"Roberts, R.J. (1989). Laboratory methods. Fish Pathology, Bailli\u00e8re Tindall. [2nd ed.]."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Gruber-Dorninger, C., M\u00fcller, A., and Rosen, R. (2025). Multi-mycotoxin contamination of aquaculture feed: A global survey. Toxins, 17.","DOI":"10.3390\/toxins17030116"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Liu, Y., Luo, S., Zou, X., He, W., Tan, R., Jin, Y., Liu, G., Duan, Q., Yang, W., and Chen, Y. (2026). Low-dose deoxynivalenol induces subclinical multi-organ toxicity in weaned piglets. Toxins, 18.","DOI":"10.3390\/toxins18020075"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2759","DOI":"10.1039\/D5FO05222J","article-title":"Dietary \u03b2-sitosterol alleviates reproductive toxicity induced by combined exposure of low-dose deoxynivalenol and high-fat diet in male C57BL\/6J mice","volume":"17","author":"Zhang","year":"2026","journal-title":"Food Funct."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"133910","DOI":"10.1016\/j.chemosphere.2022.133910","article-title":"Fumonisin B1 induces hepatotoxicity in mice through the activation of oxidative stress, apoptosis and fibrosis","volume":"296","author":"Cao","year":"2022","journal-title":"Chemosphere"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"10603","DOI":"10.1021\/acs.jafc.5c01735","article-title":"Effect of fumonisin B1 and hydrolyzed FB1 exposure on intestinal and hepatic toxicity in BALB\/c mice","volume":"73","author":"Ye","year":"2025","journal-title":"J. Agric. Food Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.aquaculture.2019.02.032","article-title":"The effects of naturally occurring or purified deoxynivalenol (DON) on growth performance, nutrient utilization and histopathology of rainbow trout (Oncorhynchus mykiss)","volume":"505","author":"Hooft","year":"2019","journal-title":"Aquaculture"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"139148","DOI":"10.1016\/j.jhazmat.2025.139148","article-title":"Integrative analysis of microbiome and metabolome reveals the effect of deoxynivalenol on growth performance, liver and intestinal health of largemouth bass (Micropterus salmoides)","volume":"495","author":"Yin","year":"2025","journal-title":"J. Hazard. Mater."},{"key":"ref_45","unstructured":"D\u00f6ll, S., Valenta, H., Baardsen, G., M\u00f6ller, P., Koppe, W., Stubhaug, I., and D\u00e4nicke, S. (June, January 30). Effects of increasing concentrations of deoxynivalenol, zearalenone and ochratoxin A in diets for Atlantic salmon (Salmo salar) on performance, health and toxin residues. Proceedings of the 33rd Mycotoxin Workshop, Freising, Germany."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1016\/j.fct.2018.08.079","article-title":"Effects of dietary deoxynivalenol or ochratoxin A on performance and selected health indices in Atlantic salmon (Salmo salar)","volume":"121","author":"Bernhoft","year":"2018","journal-title":"Food Chem. Toxicol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1111\/jwas.12730","article-title":"Effect of low levels of fumonisin contamination on gilthead seabream (Sparus aurata)","volume":"51","author":"Dias","year":"2020","journal-title":"J. World Aquac. Soc."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Mello, F.V., Pereira, C., \u00d6zkan, B., Maulvault, A.L., Soares, F., Pous\u00e3o-Ferreira, P., Fernandes, J.O., Cunha, S.C., Marques, A., and Anacleto, P. (2025). Toxicological responses of juvenile gilthead seabream to enniatin B and fumonisin B1. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26125676"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.aquaculture.2019.04.027","article-title":"Fumonisin affects performance and modulates the gene expression of IGF-1 and GHR in Nile tilapia fingerlings and juveniles","volume":"507","author":"Lala","year":"2019","journal-title":"Aquaculture"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"14486","DOI":"10.1016\/S0021-9258(18)98712-0","article-title":"Inhibition of sphingolipid biosynthesis by fumonisins. Implications for diseases associated with Fusarium moniliforme","volume":"266","author":"Wang","year":"1991","journal-title":"J. Biol. Chem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1006\/taap.1996.0119","article-title":"Elevated sphingoid bases and complex sphingolipid depletion as contributing factors in fumonisin-induced cytotoxicity","volume":"138","author":"Yoo","year":"1996","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/0166-445X(94)00050-6","article-title":"Sphingolipid levels in catfish consuming Fusarium moniliforme corn culture material containing fumonisins","volume":"30","author":"Goel","year":"1994","journal-title":"Aquat. Toxicol."},{"key":"ref_53","unstructured":"European Commission (2006). Commission Recommendation 2006\/576\/EC of 17 August 2006 on the presence of deoxynivalenol, zearalenone, ochratoxin A, T-2 and HT-2 and fumonisins in products intended for animal feeding. Off. J. Eur. Union, L229, 7\u20139."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Koletsi, P., Wiegertjes, G.F., Graat, E.A.M., Lyons, P., and Schrama, J. (2022). Time- and dose-dependent effects of dietary deoxynivalenol (DON) in rainbow trout (Oncorhynchus mykiss) at organism and tissue level. Toxins, 14.","DOI":"10.3390\/toxins14110810"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1007\/s12550-023-00496-0","article-title":"Individual and combined effects of deoxynivalenol (DON) with other Fusarium mycotoxins on rainbow trout (Oncorhynchus mykiss) growth performance and health","volume":"39","author":"Koletsi","year":"2023","journal-title":"Mycotoxin Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"756","DOI":"10.3390\/toxins6020756","article-title":"Organ damage and hepatic lipid accumulation in carp (Cyprinus carpio L.) after feed-borne exposure to the mycotoxin deoxynivalenol (DON)","volume":"6","author":"Pietsch","year":"2014","journal-title":"Toxins"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"100326","DOI":"10.1016\/j.aqrep.2020.100326","article-title":"Evaluation of growth performance, hematological, biochemical and histopathological parameters of Rhamdia quelen fed with a feed artificially contaminated with aflatoxin B1","volume":"17","author":"Anater","year":"2020","journal-title":"Aquac. Rep."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Lin, Y., Wang, R., Liu, S., Zhao, H., Wen, B., Chen, S., Guo, R., Wang, L., Xia, X., and Xu, Y. (2025). Deoxynivalenol and its metabolites: Contamination, metabolism, and toxicity. Toxins, 17.","DOI":"10.3390\/toxins17110555"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Witeska, M., Kondera, E., and Bojarski, B. (2023). Hematological and hematopoietic analysis in fish toxicology\u2014A review. Animals, 13.","DOI":"10.3390\/ani13162625"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"33933","DOI":"10.18632\/oncotarget.15422","article-title":"T-2 mycotoxin: Toxicological effects and decontamination strategies","volume":"8","author":"Adhikari","year":"2017","journal-title":"Oncotarget"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"R646","DOI":"10.1152\/ajpregu.00247.2017","article-title":"The differential role of reactive oxygen species in early and late stages of cancer","volume":"313","author":"Assi","year":"2017","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"145","DOI":"10.3329\/jsr.v12i1.41716","article-title":"Oxidative stress in fish: A review","volume":"12","author":"Chowdhury","year":"2020","journal-title":"J. Sci. Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1016\/j.scitotenv.2008.10.004","article-title":"Gill and head kidney antioxidant processes and innate immune system responses of yellow perch (Perca flavescens) exposed to different contaminants in the St. Lawrence River, Canada","volume":"407","author":"Dautremepuits","year":"2009","journal-title":"Sci. Total Environ."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1006\/eesa.1994.1026","article-title":"Effects of lindane exposure on rainbow trout (Oncorhynchus mykiss) immunity. III. Effect on nonspecific immunity and B lymphocyte functions","volume":"27","author":"Dunier","year":"1994","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_65","first-page":"105","article-title":"Effects of subchronic exposure to Spartakus (prochloraz) on common carp Cyprinus carpio","volume":"31","author":"Haluzova","year":"2010","journal-title":"Neuro Endocrinol. Lett."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Balabanova, L., Bondarev, G., Seitkalieva, A., Son, O., and Tekutyeva, L. (2024). Insights into alkaline phosphatase anti-inflammatory mechanisms. Biomedicines, 12.","DOI":"10.3390\/biomedicines12112502"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"60","DOI":"10.5633\/amm.2022.0208","article-title":"Blue moonlighting in the immune response: Roles of copper and ceruloplasmin in the pathogenesis of inflammation and immune-mediated diseases","volume":"61","author":"Petrovski","year":"2022","journal-title":"Acta Med. Median."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Wentzel, A.S., Janssen, J.J.E., de Boer, V.C.J., van Veen, W.G., Forlenza, M., and Wiegertjes, G.F. (2020). Fish macrophages show distinct metabolic signatures upon polarization. Front. Immunol., 11.","DOI":"10.3389\/fimmu.2020.00152"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.tox.2005.05.010","article-title":"Comparative study of cytotoxicity and oxidative stress induced by deoxynivalenol, zearalenone or fumonisin B1 in human intestinal cell line Caco-2","volume":"213","author":"Kouadio","year":"2005","journal-title":"Toxicology"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s00204-015-1604-8","article-title":"Fumonisins: Oxidative stress-mediated toxicity and metabolism in vivo and in vitro","volume":"90","author":"Wang","year":"2016","journal-title":"Arch. Toxicol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.1080\/02652030802056626","article-title":"Mechanisms of deoxynivalenol-induced gene expression and apoptosis","volume":"25","author":"Pestka","year":"2008","journal-title":"Food Addit. Contam. Part A Chem. Anal. Control Expo. Risk Assess."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"112397","DOI":"10.1016\/j.fct.2021.112397","article-title":"Hypothesis: Long non-coding RNA is a potential target of mycotoxins","volume":"155","author":"You","year":"2021","journal-title":"Food Chem. Toxicol."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Domijan, A.M., Hercog, K., \u0160tampar, M., Gajski, G., Geri\u0107, M., Sokolovi\u0107, M., and \u017degura, B. (2023). Impact of deoxynivalenol and zearalenone as single and combined treatment on DNA, cell cycle and cell proliferation in HepG2 cells. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24044082"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"120625","DOI":"10.1016\/j.envpol.2022.120625","article-title":"A novel toxic effect of foodborne trichothecenes: The exacerbation of genotoxicity","volume":"317","author":"Garofalo","year":"2023","journal-title":"Environ. Pollut."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"e00007-17","DOI":"10.1128\/mBio.00007-17","article-title":"The food contaminant deoxynivalenol exacerbates the genotoxicity of gut microbiota","volume":"8","author":"Payros","year":"2017","journal-title":"mBio"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"3548","DOI":"10.1016\/j.fct.2011.12.022","article-title":"DNA damage in organs of mice treated acutely with patulin, a known mycotoxin","volume":"50","author":"Greggio","year":"2012","journal-title":"Food Chem. Toxicol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"113","DOI":"10.3920\/WMJ2017.2267","article-title":"Mycotoxins and oxidative stress: Where are we?","volume":"11","author":"Bracarense","year":"2018","journal-title":"World Mycotoxin J."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.marpolbul.2011.11.026","article-title":"Oxidative stress, genotoxicity and histopathology biomarker responses in mullet (Mugil cephalus) and sea bass (Dicentrarchus labrax) liver from Bizerte Lagoon (Tunisia)","volume":"64","author":"Barhoumi","year":"2012","journal-title":"Mar. Pollut. Bull."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.aquatox.2010.10.006","article-title":"Environmentally induced oxidative stress in aquatic animals","volume":"101","author":"Lushchak","year":"2011","journal-title":"Aquat. Toxicol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"112616","DOI":"10.1016\/j.fct.2021.112616","article-title":"Deoxynivalenol: Mechanisms of action and its effects on various terrestrial and aquatic species","volume":"157","author":"Hooft","year":"2021","journal-title":"Food Chem. Toxicol."},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Ma, W., Zeng, W., Zhang, D., Zhou, Y., Huang, Y., and Hong, Y. (2025). Oxidative stress in aquaculture: Pathogenic mechanisms and preventive strategies in farmed aquatic animals. Curr. Issues Mol. Biol., 47.","DOI":"10.3390\/cimb47110873"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"104154","DOI":"10.1016\/j.etap.2023.104154","article-title":"Role of epigenetics in mycotoxin toxicity: A review","volume":"100","author":"Li","year":"2023","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1111\/jwas.12542","article-title":"Effects of deoxynivalenol exposure time and contamination levels on rainbow trout","volume":"50","author":"Eder","year":"2019","journal-title":"J. World Aquac. Soc."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"310680","DOI":"10.1155\/2014\/310680","article-title":"The effect of mycotoxin deoxynivalenol on haematological and biochemical indicators and histopathological changes in rainbow trout (Oncorhynchus mykiss)","volume":"2014","author":"Matejova","year":"2014","journal-title":"BioMed Res. Int."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/0044-8486(94)00219-E","article-title":"Growth, hematology, and histopathology of channel catfish, Ictalurus punctatus, fed toxins from Fusarium moniliforme","volume":"130","author":"Lumlertdacha","year":"1995","journal-title":"Aquaculture"},{"key":"ref_86","first-page":"52","article-title":"Histopathological outcomes designating the toxicological aspects of fumonisin B1 on cultured Nile tilapia, Oreochromis niloticus","volume":"4","author":"Khalil","year":"2016","journal-title":"Int. J. Fish. Aquat. Stud."},{"key":"ref_87","first-page":"358","article-title":"Fumonisin B1 causes multiple lesions in common carp (Cyprinus carpio)","volume":"111","author":"Petrinec","year":"2004","journal-title":"Dtsch. Tierarztl. Wochenschr."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Fornari, D.C., Peixoto, S., Ksepka, S.P., Bullard, S.A., Rossi, W., Nuzback, D.E., and Davis, D.A. (2023). Effects of dietary mycotoxins and mycotoxin adsorbent additives on production performance, hematological parameters, and liver histology in juvenile Nile tilapia (Oreochromis niloticus). Front. Anim. Sci., 4.","DOI":"10.3389\/fanim.2023.1281722"}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/16\/9\/1258\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T10:32:45Z","timestamp":1788431565000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/16\/9\/1258"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,31]]},"references-count":88,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["biom16091258"],"URL":"https:\/\/doi.org\/10.3390\/biom16091258","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,31]]}}}