{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T09:07:56Z","timestamp":1788944876059,"version":"build-2803163510"},"reference-count":133,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"Korean government","doi-asserted-by":"publisher","award":["RS-2026-25481309"],"award-info":[{"award-number":["RS-2026-25481309"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>The clustered regularly interspaced short palindromic repeats (CRISPR)\/CRISPR-associated protein (Cas) system began as a tool for programmable genome editing. CRISPR\/Cas technologies have evolved into a versatile platform for functional genomic screening, epigenome editing, therapeutic target discovery, and highly sensitive molecular diagnostics. New effectors and engineered variants continue to push these applications into personalized medicine and point-of-care testing. Here, this review highlights recent advances in therapeutic target discovery and therapeutic and molecular diagnostic development utilizing CRISPR\/Cas technologies. We discuss how CRISPR interference (CRISPRi), CRISPR activation (CRISPRa), base editing, and prime editing have improved our understanding of disease mechanisms, while creating new opportunities for therapeutic intervention. Current applications in cancer immunotherapy, infectious disease, and neurological disorders are also discussed. In diagnostics, CRISPR-based platforms enable sensitive detection of infectious pathogens, cancer biomarkers, and genetic disorders using programmable nuclease activity in both laboratory and point-of-care settings. Collectively, these advances continue to expand the role of CRISPR\/Cas technology across biological discovery, disease diagnostics, and therapeutic development, driving progress in precision medicine.<\/jats:p>","DOI":"10.3390\/biom16091268","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T15:39:47Z","timestamp":1788363587000},"page":"1268","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Recent Advances in CRISPR\/Cas Technologies for Biological Discovery, Therapeutics, and Diagnostics"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6778-9881","authenticated-orcid":false,"given":"Moon-Soo","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Innovative Drug Discovery and Development, College of Pharmacy, Keimyung University, 10, Daegu 42601, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hae Sol","family":"Do","sequence":"additional","affiliation":[{"name":"Department of Innovative Drug Discovery and Development, College of Pharmacy, Keimyung University, 10, Daegu 42601, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye Yeon","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Innovative Drug Discovery and Development, College of Pharmacy, Keimyung University, 10, Daegu 42601, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang Eun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Innovative Drug Discovery and Development, College of Pharmacy, Keimyung University, 10, Daegu 42601, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sun Ju","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Innovative Drug Discovery and Development, College of Pharmacy, Keimyung University, 10, Daegu 42601, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5429","DOI":"10.1128\/jb.169.12.5429-5433.1987","article-title":"Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product","volume":"169","author":"Ishino","year":"1987","journal-title":"J. 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