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Bats are the only mammals capable of sustained powered flight, accompanied by extreme metabolic demands and a nocturnal lifestyle. However, the regulatory mechanisms underlying hepatic circadian rhythms in bats remain poorly understood. Herein, we employed data-independent acquisition (DIA)-based quantitative proteomics to characterize the diurnal dynamics of hepatic proteins in Vespertilio sinensis across four representative physiological states, and further conducted integrative multi-omics analysis combined with transcriptomic data. The results revealed that approximately 5% of hepatic proteins exhibited circadian rhythmicity in V. sinensis, a proportion markedly higher than that of rhythmic transcripts and metabolites. Prominent transcription-protein rhythm decoupling was observed, implying that post-transcriptional regulatory processes may play critical roles in shaping circadian rhythms. Core metabolic pathways displayed temporally dynamic enrichment. The diurnal functional switching of the liver was achieved via stable expression of core proteins and temporal turnover of specific proteins, which efficiently coordinated energy supply, oxidative defense and detoxification metabolism. Trend clustering and functional enrichment analyses suggested that the liver may undergo lysosome-mediated immune repair during the daytime, while exhibiting enriched energy metabolic profiles that potentially support the elevated energy expenditure required for flight at night. This study systematically elucidates the protein regulatory patterns underlying hepatic diurnal rhythms in V. sinensis, and provides molecular clues for exploring extreme energy adaptation and circadian evolutionary scenarios in nocturnal flying mammals.<\/jats:p>","DOI":"10.3390\/biom16091289","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T08:07:06Z","timestamp":1788768426000},"page":"1289","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dynamic Atlas and Energy Metabolism Adaptation of the Liver Proteome During a 24 h Cycle in Vespertilio sinensis"],"prefix":"10.3390","volume":"16","author":[{"given":"Tianhui","family":"Wang","sequence":"first","affiliation":[{"name":"College of Life Science, Jilin Agricultural University, Changchun 130118, China"},{"name":"Jilin Provincial International Cooperation Key Laboratory for Biological Control of Agricultural Pests, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Yujia","family":"Chu","sequence":"additional","affiliation":[{"name":"College of Life Science, Jilin Agricultural University, Changchun 130118, China"},{"name":"Jilin Provincial International Cooperation Key Laboratory for Biological Control of Agricultural Pests, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Xin","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Science, Jilin Agricultural University, Changchun 130118, China"},{"name":"Jilin Provincial International Cooperation Key Laboratory for Biological Control of Agricultural Pests, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Haokun","family":"Zheng","sequence":"additional","affiliation":[{"name":"College of Life Science, Jilin Agricultural University, Changchun 130118, China"},{"name":"Jilin Provincial International Cooperation Key Laboratory for Biological Control of Agricultural Pests, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Jiang","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Life Science, Jilin Agricultural University, Changchun 130118, China"},{"name":"Jilin Provincial International Cooperation Key Laboratory for Biological Control of Agricultural Pests, Jilin Agricultural University, Changchun 130118, China"},{"name":"Jilin Provincial Key Laboratory of Animal Resource and Ecological Security, Northeast Normal University, Changchun 130117, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"resources","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8753-9372","authenticated-orcid":false,"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Science, Jilin Agricultural University, Changchun 130118, China"},{"name":"Jilin Provincial International Cooperation Key Laboratory for Biological Control of Agricultural Pests, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"supervision","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1126\/science.aah4967","article-title":"Circadian physiology of metabolism","volume":"354","author":"Panda","year":"2016","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Bautista, J., Ojeda-Mosquera, S., Ordonez-Lozada, D., and Lopez-Cortes, A. (2025). Peripheral clocks and systemic zeitgeber interactions: From molecular mechanisms to circadian precision medicine. Front. Endocrinol., 16.","DOI":"10.3389\/fendo.2025.1606242"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1002\/1873-3468.70150","article-title":"Biochemical mechanism of the mammalian circadian clock","volume":"600","author":"Liu","year":"2026","journal-title":"FEBS Lett."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1038\/s41580-019-0179-2","article-title":"Molecular mechanisms and physiological importance of circadian rhythms","volume":"21","author":"Patke","year":"2020","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1038\/s42003-025-07932-0","article-title":"The metabolic significance of peripheral tissue clocks","volume":"8","author":"Hunter","year":"2025","journal-title":"Commun. Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1038\/s41580-018-0096-9","article-title":"Crosstalk between metabolism and circadian clocks","volume":"20","author":"Reinke","year":"2019","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2667","DOI":"10.1007\/s00018-014-1574-7","article-title":"Circadian clocks and energy metabolism","volume":"71","author":"Sancar","year":"2014","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"C1158","DOI":"10.1152\/ajpcell.00551.2022","article-title":"Circadian regulation of liver metabolism: Experimental approaches in human, rodent, and cellular models","volume":"325","author":"Daniels","year":"2023","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0092-8674(02)00722-5","article-title":"Coordinated transcription of key pathways in the mouse by the circadian clock","volume":"109","author":"Panda","year":"2002","journal-title":"Cell"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Robles, M.S., Cox, J., and Mann, M. (2014). In-vivo quantitative proteomics reveals a key contribution of post-transcriptional mechanisms to the circadian regulation of liver metabolism. PLoS Genet., 10.","DOI":"10.1371\/journal.pgen.1004047"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.molcel.2025.12.020","article-title":"A mouse circadian proteome atlas","volume":"86","author":"Otobe","year":"2026","journal-title":"Mol. Cell"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1073\/pnas.1314066111","article-title":"Circadian clock-dependent and -independent rhythmic proteomes implement distinct diurnal functions in mouse liver","volume":"111","author":"Mauvoisin","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"O111.016717","DOI":"10.1074\/mcp.O111.016717","article-title":"Targeted Data Extraction of the MS\/MS Spectra Generated by Data-independent Acquisition: A New Concept for Consistent and Accurate Proteome Analysis","volume":"11","author":"Gillet","year":"2012","journal-title":"Mol. Cell. Proteom."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1126\/science.1230835","article-title":"Comparative analysis of bat genomes provides insight into the evolution of flight and immunity","volume":"339","author":"Zhang","year":"2013","journal-title":"Science"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1146\/annurev-animal-022516-022811","article-title":"Bat1K Consortium. Bat Biology, Genomes, and the Bat1K Project: To Generate Chromosome-Level Genomes for All Living Bat Species","volume":"6","author":"Teeling","year":"2018","journal-title":"Annu. Rev. Anim. Biosci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1146\/annurev-biochem-061516-045037","article-title":"Oxidative Stress","volume":"86","author":"Sies","year":"2017","journal-title":"Annu. Rev. Biochem."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Chu, Y.J., Li, J.J., Feng, L., Zhang, G.T., Wu, H., Jiang, T.L., Wang, H., and Feng, J. (2022). Comparative analysis of the daily liver transcriptomes in wild nocturnal bats. BMC Genom., 23.","DOI":"10.1186\/s12864-022-08823-y"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1038\/nmeth.1322","article-title":"Universal sample preparation method for proteome analysis","volume":"6","author":"Wisniewski","year":"2009","journal-title":"Nat. Methods"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1016\/1044-0305(94)80016-2","article-title":"An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database","volume":"5","author":"Eng","year":"1994","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1074\/mcp.M114.044305","article-title":"Extending the Limits of Quantitative Proteome Profiling with Data-Independent Acquisition and Application to Acetaminophen-Treated Three-Dimensional Liver Microtissues","volume":"14","author":"Bruderer","year":"2015","journal-title":"Mol. Cell. Proteom."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"W585","DOI":"10.1093\/nar\/gkm259","article-title":"WoLF PSORT: Protein localization predictor","volume":"35","author":"Horton","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.jprot.2015.09.013","article-title":"MapDIA: Preprocessing and statistical analysis of quantitative proteomics data from data independent acquisition mass spectrometry","volume":"129","author":"Teo","year":"2015","journal-title":"J. Proteom."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Jiang, Y., Liu, C., and Zhong, Y. (2025). Temporal Orchestration of Immunity: How Circadian Clocks Coordinate Immune Responses. Front. Biosci. Landmark, 30.","DOI":"10.31083\/FBL39948"},{"key":"ref_24","unstructured":"Berg, J.M., Tymoczko, J.L., Gatto, G.J., and Lubert, L. (2019). Biochemistry, W. H. Freeman. [9th ed.]."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1080\/03602532.2018.1555587","article-title":"Aldehyde toxicity and metabolism: The role of aldehyde dehydrogenases in detoxification, drug resistance and carcinogenesis","volume":"51","author":"Laskar","year":"2019","journal-title":"Drug Metab. Rev."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"101363","DOI":"10.1016\/j.redox.2019.101363","article-title":"Impaired inflammasome activation and bacterial clearance in G6PD deficiency due to defective NOX\/p38 MAPK\/AP-1 redox signaling","volume":"28","author":"Yen","year":"2019","journal-title":"Redox Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1093\/cz\/zoy039","article-title":"Migratory flight imposes oxidative stress in bats","volume":"65","author":"Costantini","year":"2019","journal-title":"Curr. Zool."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1038\/s42255-023-00863-2","article-title":"The pentose phosphate pathway in health and disease","volume":"5","author":"TeSlaa","year":"2023","journal-title":"Nat. Metab."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.livres.2023.07.002","article-title":"Retinoic acid signaling in fatty liver disease","volume":"7","author":"Bawa","year":"2023","journal-title":"Liver Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"250092","DOI":"10.1098\/rsob.250092","article-title":"Brain amino acid sensing for organismal amino acid homeostasis","volume":"15","author":"Tsang","year":"2025","journal-title":"Open Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1016\/S0140-6736(02)11203-7","article-title":"Clinical importance of the cytochromes P450","volume":"360","author":"Nebert","year":"2002","journal-title":"Lancet"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15616\/BSL.2026.32.1.1","article-title":"The Bat Blueprint: Translating Mitochondrial Adaptations into Next-generation Therapeutics","volume":"32","author":"An","year":"2026","journal-title":"Biomed. Sci. Lett."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Pereira, F.D., Mena Canata, D.A., Salomon, T.B., Hackenhaar, F.S., Pereira, M.J.R., Benfato, M.S., and Rampelotto, P.H. (2023). Oxidative Stress and Antioxidant Defense in the Heart, Liver, and Kidney of Bat Species with Different Feeding Habits. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms242216369"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Onken, B., Kalinava, N., and Driscoll, M. (2020). Gluconeogenesis and PEPCK are critical components of healthy aging and dietary restriction life extension. PLoS Genet., 16.","DOI":"10.1371\/journal.pgen.1008982"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.trsl.2023.12.003","article-title":"Aldehyde dehydrogenase 2 serves as a key cardiometabolic adaptation regulator in response to plateau hypoxia in mice","volume":"267","author":"Gao","year":"2024","journal-title":"Transl. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.freeradbiomed.2020.01.183","article-title":"Aldehyde dehydrogenase 3A1 confers oxidative stress resistance accompanied by altered DNA damage response in human corneal epithelial cells","volume":"150","author":"Voulgaridou","year":"2020","journal-title":"Free Radic. Biol. Med."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1038\/s41586-020-03128-0","article-title":"Lessons from the host defences of bats, a unique viral reservoir","volume":"589","author":"Irving","year":"2021","journal-title":"Nature"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"20210211","DOI":"10.1098\/rsif.2021.0211","article-title":"Role of body temperature variations in bat immune response to viral infections","volume":"18","author":"Fumagalli","year":"2021","journal-title":"J. R. Soc. Interface"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1038\/s41564-019-0371-3","article-title":"Dampened NLRP3-mediated inflammation in bats and implications for a special viral reservoir host","volume":"4","author":"Ahn","year":"2019","journal-title":"Nat. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Pei, G., Balkema-Buschmann, A., and Dorhoi, A. (2024). Disease tolerance as immune defense strategy in bats: One size fits all?. PLoS Pathog., 20.","DOI":"10.1371\/journal.ppat.1012471"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"4468","DOI":"10.4049\/jimmunol.1502062","article-title":"Characterization of the Antigen Processing Machinery and Endogenous Peptide Presentation of a Bat MHC Class I Molecule","volume":"196","author":"Wynne","year":"2016","journal-title":"J. Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"102780","DOI":"10.1016\/j.ceca.2023.102780","article-title":"Calcium signalling in hepatic metabolism: Health and diseases","volume":"114","author":"Humbert","year":"2023","journal-title":"Cell Calcium"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e03204-23","DOI":"10.1128\/mbio.03204-23","article-title":"Bat adaptations in inflammation and cell death regulation contribute to viral tolerance","volume":"16","author":"Das","year":"2025","journal-title":"mBio"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"7998","DOI":"10.18632\/aging.202852","article-title":"Evolution of mammalian longevity: Age-related increase in autophagy in bats compared to other mammals","volume":"13","author":"Kacprzyk","year":"2021","journal-title":"Aging"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Wu, B., Lu, D., and Dong, D. (2020). Circadian Clock and CYP Metabolism. Circadian Pharmacokinetics, Springer.","DOI":"10.1007\/978-981-15-8807-5"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1080\/17425255.2024.2356167","article-title":"The role of the circadian timing system on drug metabolism and detoxification: An update","volume":"20","author":"Okyar","year":"2024","journal-title":"Expert Opin. Drug Metab. Toxicol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e71666","DOI":"10.1002\/ece3.71666","article-title":"Comparative Analysis of the Daily Liver Metabolomics of Asian Particolored Bat","volume":"15","author":"Chu","year":"2025","journal-title":"Ecol. Evol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.cmet.2016.10.003","article-title":"Nuclear Proteomics Uncovers Diurnal Regulatory Landscapes in Mouse Liver","volume":"25","author":"Wang","year":"2017","journal-title":"Cell Metab."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1038\/s41467-018-03898-2","article-title":"A proteomics landscape of circadian clock in mouse liver","volume":"9","author":"Wang","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Jouffe, C., Cretenet, G., Symul, L., Martin, E., Atger, F., Naef, F., and Gachon, F. (2013). The circadian clock coordinates ribosome biogenesis. PLoS Biol., 11.","DOI":"10.1371\/journal.pbio.1001455"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"D1211","DOI":"10.1093\/nar\/gky869","article-title":"iProX: An integrated proteome resource","volume":"47","author":"Ma","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"D1522","DOI":"10.1093\/nar\/gkab1081","article-title":"iProX in 2021: Connecting proteomics data sharing with big data","volume":"50","author":"Chen","year":"2022","journal-title":"Nucleic Acids Res."}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/16\/9\/1289\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T12:35:59Z","timestamp":1789130159000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/16\/9\/1289"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,6]]},"references-count":52,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["biom16091289"],"URL":"https:\/\/doi.org\/10.3390\/biom16091289","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,6]]}}}