{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T17:04:05Z","timestamp":1789578245524,"version":"build-2803163510"},"reference-count":94,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science Committee of the Ministry of Science and Higher Education, Republic of Kazakhstan","award":["AP23488044"],"award-info":[{"award-number":["AP23488044"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Accessions of Russian wildrye, Psathyrostachys juncea, an important pasture fodder crop, were arranged in group A with high silicon content in leaves and group B with low silicon content. Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) analyses revealed low Si accumulation in roots but higher deposition in leaves of plants from group A, whereas, in contrast, plants from group B showed significantly higher Si content in roots but lower in leaves. Three identified PjLsi genes encoding influx and efflux of silicon were differentially expressed, with higher levels in plants from group A, including PjLsi1 and PjLsi3 in leaves, and PjLsi2 in roots, compared to those from group B. A working model was proposed based on differences in microscopy and gene expression analyses. Sanger sequencing revealed one SNP in the conservative gene PjLsi1, and 14 and 29 SNPs in the more polymorphic DNA sequences of PjLsi2 and PjLsi3, respectively. Allele-specific qPCR (ASQ) markers were developed based on optimally identified SNPs and successfully used for genotyping of all three PjLsi genes in a germplasm collection comprising 72 P. juncea accessions. Highly significant differences were found between groups of genotypes in all three PjLsi genes using ASQ markers with corresponding Si content in the studied accessions, including those from groups A and B. The developed ASQ markers can be tested as candidates in marker-assisted selection for genotyping of hybrid breeding lines and for identification of the best P. juncea genotypes with minimal Si content in leaves of pasture fodder crop.<\/jats:p>","DOI":"10.3390\/biom16091351","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T16:07:29Z","timestamp":1789574849000},"page":"1351","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Low Silicon Content in Leaves and Higher Deposition in Roots Is Associated with Lsi Genes in Psathyrostachys juncea: Microscopy, Gene Expression and Genotyping Study"],"prefix":"10.3390","volume":"16","author":[{"given":"Svetlana","family":"Dashkevich","sequence":"first","affiliation":[{"name":"A.I.Barayev Research and Production Centre of Grain Farming, Shortandy 021601, Akmola Region, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0729-0592","authenticated-orcid":false,"given":"Maral","family":"Utebayev","sequence":"additional","affiliation":[{"name":"A.I.Barayev Research and Production Centre of Grain Farming, Shortandy 021601, Akmola Region, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadezhda","family":"Filippova","sequence":"additional","affiliation":[{"name":"A.I.Barayev Research and Production Centre of Grain Farming, Shortandy 021601, Akmola Region, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4904-2837","authenticated-orcid":false,"given":"Oksana","family":"Kradetskaya","sequence":"additional","affiliation":[{"name":"A.I.Barayev Research and Production Centre of Grain Farming, Shortandy 021601, Akmola Region, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Chilimova","sequence":"additional","affiliation":[{"name":"A.I.Barayev Research and Production Centre of Grain Farming, Shortandy 021601, Akmola Region, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2182-8977","authenticated-orcid":false,"given":"Botagoz","family":"Sharipova","sequence":"additional","affiliation":[{"name":"Biology Department, Sh.Ualikhanov University, Kokshetau 020000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gulmira","family":"Khassanova","sequence":"additional","affiliation":[{"name":"Institute of Agronomy and Forestry, Saken Seifullin Kazakh Agrotechnical University, Astana 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1049-4011","authenticated-orcid":false,"given":"Marzhan","family":"Kuzbakova","sequence":"additional","affiliation":[{"name":"Institute of Agronomy and Forestry, Saken Seifullin Kazakh Agrotechnical University, Astana 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satyvaldy","family":"Jatayev","sequence":"additional","affiliation":[{"name":"Institute of Agronomy and Forestry, Saken Seifullin Kazakh Agrotechnical University, Astana 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7748-5673","authenticated-orcid":false,"given":"Nurgul","family":"Daniyeva","sequence":"additional","affiliation":[{"name":"Core Facilities, Nazarbayev University, Astana 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2941-0340","authenticated-orcid":false,"given":"Yuri","family":"Shavrukov","sequence":"additional","affiliation":[{"name":"College of Science and Engineering, Biological Sciences, Flinders University, Adelaide, SA 5042, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Del-Claro, K., and Torezan-Silingardi, H.M. (2021). The genetic basis of plant-herbivore interactions. Plant-Animal Interactions: Source of Biodiversity, Springer.","DOI":"10.1007\/978-3-030-66877-8"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2439248","DOI":"10.1080\/15592324.2024.2439248","article-title":"The biochemical and molecular mechanisms of plants: A review on insect herbivory","volume":"20","author":"Adedayo","year":"2025","journal-title":"Plant Signal. Behav."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Chamani, M., Dadpour, M., Dehghanian, Z., Panahirad, S., Chenari Bouket, A., Oszako, T., and Kumar, S. (2025). From digestion to detoxification: Exploring plant metabolite impacts on insect enzyme systems for enhanced pest control. Insects, 16.","DOI":"10.3390\/insects16040392"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"100850","DOI":"10.1016\/j.stress.2025.100850","article-title":"Phytohormonal signaling network and immune priming pertinence in plants to defend against insect herbivory","volume":"16","author":"Kolanchi","year":"2025","journal-title":"Plant Stress"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1093\/mp\/ssu049","article-title":"Growth-defense tradeoffs in plants: A balancing act to optimize fitness","volume":"7","author":"Huot","year":"2014","journal-title":"Mol. Plant"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1007\/s13744-025-01300-7","article-title":"Host plant resistance: Is it time for a new model?","volume":"54","author":"Souza","year":"2025","journal-title":"Neotrop. Entomol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3474","DOI":"10.1111\/1365-2435.70206","article-title":"Grazing disrupts the trade-off between silica- and phenol-based plant defences along an aridity gradient in grasslands","volume":"39","author":"Yan","year":"2025","journal-title":"Funct. Ecol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1007\/s00267-002-2608-8","article-title":"Riparian livestock exclosure research in the Western United States: A critique and some recommendations","volume":"30","author":"Sarr","year":"2002","journal-title":"Environ. Manag."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"416","DOI":"10.2135\/cropsci2006.01.0061","article-title":"Interaction of plant species diversity on grazing behavior and performance of livestock grazing temperate region pastures","volume":"47","author":"Soder","year":"2007","journal-title":"Crop Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1071\/CP13284","article-title":"Perennial pasture grasses\u2014An historical review of their introduction, use and development for southern Australia","volume":"65","author":"Reed","year":"2014","journal-title":"Crop Pasture Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/S0065-2113(08)00206-X","article-title":"Genetic improvement of forage species to reduce the environmental impact of temperate livestock grazing systems","volume":"98","author":"Abberton","year":"2008","journal-title":"Adv. Agron."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Moser, L.E., Buxton, D.R., and Casler, M.D. (1996). Wildryes. Cool-Season Forage Grasses, American Society of Agronomy, Crop Science Society of America and Soil Science Society of America.","DOI":"10.2134\/agronmonogr34"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1890","DOI":"10.2135\/cropsci1999.3961890x","article-title":"Variation in agronomic and morphological traits among Russian wildrye accessions","volume":"39","author":"Berdahl","year":"1999","journal-title":"Crop Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.tplants.2020.10.005","article-title":"Silicon defence in plants: Does herbivore identity matter?","volume":"26","author":"Johnson","year":"2021","journal-title":"Trends Plant Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3912","DOI":"10.1093\/jxb\/eraf102","article-title":"The potential of silicon in crop protection against phloem feeding and chewing insect pests: A review","volume":"76","author":"Bathoova","year":"2025","journal-title":"J. Exp. Bot."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1007\/s11738-022-03365-y","article-title":"Silicon triggers the signalling molecules and stress-associated genes for alleviating the adverse effect of terminal heat stress in wheat with improved grain quality","volume":"44","author":"Kumar","year":"2022","journal-title":"Acta Physiol. Plant."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"112669","DOI":"10.1016\/j.plantsci.2025.112669","article-title":"A comparative study of silicon uptake, accumulation and understanding its role in salt stress mitigation in millets","volume":"359","author":"Mali","year":"2025","journal-title":"Plant Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1146\/annurev-phyto-080516-035312","article-title":"Silicon\u2019s role in abiotic and biotic plant stresses","volume":"55","author":"Debona","year":"2017","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Hassan, K.M., Ajaj, R., Abdelhamid, A.N., Ebrahim, M., Hassan, I.F., Hassan, F.A.S., Alam-Eldein, S.M., and Ali, M.A.A. (2024). Silicon: A powerful aid for medicinal and aromatic plants against abiotic and biotic stresses for sustainable agriculture. Horticulturae, 10.","DOI":"10.3390\/horticulturae10080806"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Kumar, S., Soukup, M., and Elbaum, R. (2017). Silicification in grasses: Variation between different cell types. Front. Plant Sci., 8.","DOI":"10.3389\/fpls.2017.00438"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1034\/j.1399-3054.2002.1150110.x","article-title":"Silicification in sorghum (Sorghum bicolor) cultivars with different drought tolerance","volume":"115","author":"Lux","year":"2002","journal-title":"Physiol. Plant."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1016\/j.tplants.2025.03.004","article-title":"Harnessing plant silicon defenses for biological control of herbivorous insects","volume":"30","author":"Islam","year":"2025","journal-title":"Trends Plant Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"5363","DOI":"10.1093\/jxb\/erad224","article-title":"Induction of silicon defences in wheat landraces is local, not systemic, and driven by mobilization of soluble silicon to damaged leaves","volume":"74","author":"Thorne","year":"2023","journal-title":"J. Exp. Bot."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Ryalls, J.M.W., Moore, B.D., and Johnson, S.N. (2018). Silicon uptake by a pasture grass experiencing simulated grazing is greatest under elevated precipitation. BMC Ecol., 18.","DOI":"10.1186\/s12898-018-0208-6"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Quigley, K.M., and Anderson, T.M. (2014). Leaf silica concentration in Serengeti grasses increases with watering but not clipping: Insights from a common garden study and literature review. Front. Plant Sci., 5.","DOI":"10.3389\/fpls.2014.00568"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e14519","DOI":"10.1111\/ele.14519","article-title":"Plant silicon defences suppress herbivore performance, but mode of feeding is key","volume":"27","author":"Johnson","year":"2024","journal-title":"Ecol. Lett."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"151447","DOI":"10.1016\/j.flora.2019.151447","article-title":"Anatomical traits, silica content and herbivory damage in leaves of invasive tree Ligustrum lucidum developed in novel and native ranges","volume":"258","author":"Montti","year":"2019","journal-title":"Flora"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/s10681-020-02663-1","article-title":"Prospects to select tall fescue with a low silica concentration","volume":"216","author":"Cougnon","year":"2020","journal-title":"Euphytica"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Hartley, S.E., Fitt, R.N., McLarnon, E.L., and Wade, R.N. (2015). Defending the leaf surface: Intra-and inter-specific differences in silicon deposition in grasses in response to damage and silicon supply. Front. Plant Sci., 6.","DOI":"10.3389\/fpls.2015.00035"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Hall, C.R., Dagg, V., Waterman, J.M., and Johnson, S.N. (2020). Silicon alters leaf surface morphology and suppresses insect herbivory in a model grass species. Plants, 9.","DOI":"10.3390\/plants9050643"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1111\/nph.14867","article-title":"Interplay between silica deposition and viability during the life span of sorghum silica cells","volume":"217","author":"Kumar","year":"2018","journal-title":"New Phytol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1002\/jpln.201800373","article-title":"Genotypic differences in shoot silicon concentration and the impact on grain arsenic concentration in rice","volume":"182","author":"Talukdar","year":"2019","journal-title":"J. Plant Nutr. Soil Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1111\/tpj.12904","article-title":"A precise spacing between the NPA domains of aquaporins is essential for silicon permeability in plants","volume":"83","author":"Deshmukh","year":"2015","journal-title":"Plant J."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"625","DOI":"10.4141\/cjps66-105","article-title":"Silica and protein content of mixed prairie and fescue grassland vegetation and its relationship to the incidence of silica urolithiasis","volume":"46","author":"Bezeau","year":"1966","journal-title":"Can. J. Plant Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1093\/aob\/mci255","article-title":"Phylogenetic variation in the silicon composition of plants","volume":"96","author":"Hodson","year":"2005","journal-title":"Ann. Bot."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Exley, C. (2015). A possible mechanism of biological silicification in plants. Front. Plant Sci., 6.","DOI":"10.3389\/fpls.2015.00853"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3284","DOI":"10.1104\/pp.104.047365","article-title":"Characterization of the silicon uptake system and molecular mapping of the silicon transporter gene in rice","volume":"136","author":"Ma","year":"2004","journal-title":"Plant Physiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1038\/nature04590","article-title":"A silicon transporter in rice","volume":"440","author":"Ma","year":"2006","journal-title":"Nature"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/j.tplants.2006.06.007","article-title":"Silicon uptake and accumulation in higher plants","volume":"11","author":"Ma","year":"2006","journal-title":"Trends Plant Sci."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Saitoh, Y., and Suga, M. (2022). Structure and function of a silicic acid channel Lsi1. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.982068"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Pandey, S., Tripathi, D.K., Singh, V.P., Sharma, S., and Chauhan, D.K. (2023). Silicon uptake, transport, and accumulation in plants. Beneficial Chemical Elements of Plants: Recent Developments and Future Prospects, John Wiley and Sons.","DOI":"10.1002\/9781119691419"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"11401","DOI":"10.1073\/pnas.1508987112","article-title":"Orchestration of three transporters and distinct vascular structures in node for intervascular transfer of silicon in rice","volume":"112","author":"Yamaji","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2133","DOI":"10.1105\/tpc.109.067884","article-title":"Identification and characterization of maize and barley Lsi2-like silicon efflux transporters reveals a distinct silicon uptake system from that in rice","volume":"21","author":"Mitani","year":"2009","journal-title":"Plant Cell"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"991","DOI":"10.4161\/psb.6.7.15462","article-title":"Silicon efflux transporters isolated from two pumpkin cultivars contrasting in Si uptake","volume":"6","author":"Yamaji","year":"2011","journal-title":"Plant Signal. Behav."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.jplph.2016.06.011","article-title":"Identification and characterization of silicon efflux transporters in horsetail (Equisetum arvense)","volume":"200","author":"Vivancos","year":"2016","journal-title":"J. Plant Phys."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1007\/s10534-017-9992-2","article-title":"Genome-wide exploration of silicon (Si) transporter genes, Lsi1 and Lsi2 in plants; insights into Si-accumulation status\/capacity of plants","volume":"30","author":"Vatansever","year":"2017","journal-title":"BioMetals"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"124907","DOI":"10.1016\/j.chemosphere.2019.124907","article-title":"Silicon regulates the expression of vacuolar H+-pyrophosphatase 1 and decreases cadmium accumulation in rice (Oryza sativa L.)","volume":"240","author":"Cao","year":"2020","journal-title":"Chemosphere"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1111\/nph.17959","article-title":"A pericycle-localized silicon transporter for efficient xylem loading in rice","volume":"234","author":"Huang","year":"2022","journal-title":"New Phytol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1705","DOI":"10.1016\/j.ijbiomac.2022.10.099","article-title":"Genome-wide identification and functional analysis of silicon transporter family genes in moso bamboo (Phyllostachys edulis)","volume":"223B","author":"Geng","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"\u00c7al\u0131\u015fkan, M. (2020). Single nucleotide polymorphisms (SNPs) in plant genetics and breeding. The Recent Topics in Genetic Polymorphisms, InTech Open.","DOI":"10.5772\/intechopen.77777"},{"key":"ref_51","unstructured":"Abdurakhmonov, I.Y. (2020). Single nucleotide polymorphisms: A modern tool to screen plants for desirable traits. Plant Breeding\u2014Current and Future Views, IntechOpen."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Li, Z., Yun, L., Ren, X., Shi, F., and Mi, F. (2022). Analysis of controlling genes for tiller growth of Psathyrostachys juncea based on transcriptome sequencing technology. BMC Plant Biol., 22.","DOI":"10.1186\/s12870-022-03837-w"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Li, Z., Wang, T., Yun, L., Ren, X., Wang, Y., and Shi, F. (2023). Association analysis of tiller-related traits with EST-SSR markers in Psathyrostachys juncea. Genes, 14.","DOI":"10.3390\/genes14101970"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"e39105","DOI":"10.1016\/j.heliyon.2024.e39105","article-title":"GGE biplot analysis for genotype \u00d7 environment interactions affecting the seed shattering of Psathyrostachys juncea","volume":"10","author":"Lv","year":"2024","journal-title":"Heliyon"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"e18409","DOI":"10.7717\/peerj.18409","article-title":"Construction of a high-density genetic map using specific-locus amplified fragment sequencing and quantitative trait loci analysis for tillering related traits in Psathyrostachys juncea perennial grass","volume":"12","author":"Ma","year":"2024","journal-title":"PeerJ"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"e19617","DOI":"10.7717\/peerj.19617","article-title":"A SNP-based genome-wide association study (GWAS) of seed-yield related traits in Psathyrostachys juncea using wheat as a reference genome","volume":"13","author":"Li","year":"2025","journal-title":"PeerJ"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Ordon, F., and Friedt, W. (2019). Development of Single nucleotide polymorphism (SNP) markers for cereal breeding and crop research: Current methods and future prospects. Advances in Breeding Techniques for Cereal Crops, BD Publishing.","DOI":"10.1201\/9780429275463"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"565","DOI":"10.3390\/applbiosci2040036","article-title":"Agrigenomic diversity unleashed: Current single nucleotide polymorphism genotyping methods for the agricultural sciences","volume":"2","author":"Lawrie","year":"2023","journal-title":"Appl. Biosci."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Kalendar, R., Baidyussen, A., Serikbay, D., Zotova, L., Khassanova, G., Kuzbakova, M., Jatayev, S., Hu, Y.G., Schramm, C., and Anderson, P.A. (2022). Modified \u2018Allele-specific qPCR\u2019 method for SNP genotyping based on FRET. Front. Plant Sci., 12.","DOI":"10.3389\/fpls.2021.747886"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Shavrukov, Y. (2023). Modified allele-specific qPCR (ASQ) genotyping. Plant Genotyping: Methods and Protocols. Methods in Molecular Biology, Vol. 2638, Springer-Nature.","DOI":"10.1007\/978-1-0716-3024-2"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Gandhi, A., Kariyat, R., Harikishore, A., Ayati, M., Bhunia, A., and Sahoo, N. (2021). Deciphering the role of ion channels in early defense signaling against herbivorous insects. Cells, 10.","DOI":"10.3390\/cells10092219"},{"key":"ref_62","unstructured":"Ali, H., Hou, Y., and Tahir, M.B. (2023). Plant receptor interaction with herbivore-associated elicitors: Mechanisms and implications for insect herbivory-induced defense. Climate Change and Insect Biodiversity: Challenges and Implications, CRC Press."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"e15","DOI":"10.1017\/qpb.2024.19","article-title":"Silicon transport and its \u201chomeostasis\u201d in rice","volume":"5","author":"Huang","year":"2025","journal-title":"Quant. Plant Biol."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Das, G., Patra, J.K., and Baek, K.H. (2017). Insight into MAS: A molecular tool for development of stress resistant and quality of rice through gene stacking. Front. Plant Sci., 8.","DOI":"10.3389\/fpls.2017.00985"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Boopathi, N.M. (2020). Genetic Mapping and Marker Assisted Selection. Basics, Practice and Benefits, Springer-Nature.","DOI":"10.1007\/978-981-15-2949-8"},{"key":"ref_66","unstructured":"Altman, A., and Hasegawa, P.M. (2012). Marker-assisted selection in plant breeding. Plant Biotechnology and Agriculture, Academic Press."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1007\/s11103-025-01616-3","article-title":"Enhancing drought resilience in crops: Mechanistic approaches in the face of climate challenge","volume":"115","author":"Parmar","year":"2025","journal-title":"Plant Mol. Biol."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Dashkevich, S., Utebayev, M., Filippova, N., Kradetskaya, O., Chilimova, I., Rukavitsina, I., Khassanova, G., Jatayev, S., and Shavrukov, Y. (2026). Low silicon and better pasture feeding quality: Uncovering genetic diversity in Russian wildrye (Psathyrostachys juncea). Life, 16.","DOI":"10.3390\/life16040562"},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Guerriero, G., Stokes, I., Valle, N., Hausman, J., and Exley, C. (2020). Visualising silicon in plants: Histochemistry, silica sculptures and elemental imaging. Cells, 9.","DOI":"10.3390\/cells9041066"},{"key":"ref_70","unstructured":"(2025, December 01). BLASTN. Available online: https:\/\/www.genome.jp\/tools-bin\/search_sequence?prog=blastp."},{"key":"ref_71","unstructured":"(2025, December 01). Gramene. Available online: https:\/\/www.gramene.org."},{"key":"ref_72","unstructured":"(2025, December 01). CLUSTALW. Available online: https:\/\/www.genome.jp\/tools-bin\/clustalw."},{"key":"ref_73","unstructured":"(2025, December 01). 2025. University of T\u00fcbingen (Germany). Available online: https:\/\/uni-tuebingen.de\/en\/fakultaeten\/mathematisch-naturwissenschaftliche-fakultaet\/fachbereiche\/informatik\/lehrstuehle\/algorithms-in-bioinformatics\/software\/splitstree."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Li, Z., Yun, L., Gao, Z., Wang, T., Ren, X., and Zhao, Y. (2022). EST-SSR primer development and genetic structure analysis of Psathyrostachys juncea Nevski. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.837787"},{"key":"ref_75","unstructured":"(2025, December 01). NCBI, Available online: https:\/\/www.ncbi.nlm.nih.gov."},{"key":"ref_76","unstructured":"(2025, December 01). Oligo-Calculator. Available online: https:\/\/oligocalc.eu."},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Sweetman, C., Khassanova, G., Miller, T.K., Booth, N.J., Kurishbayev, A., Jatayev, S., Gupta, N.K., Langridge, P., Jenkins, C.L.D., and Soole, K.L. (2020). Salt-induced expression of intracellular vesicle trafficking genes, CaRab-GTP, and their association with Na+ accumulation in leaves of chickpea (Cicer arietinum L.). BMC Plant Biol., 20.","DOI":"10.1186\/s12870-020-02331-5"},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Khassanova, G., Kurishbayev, A., Jatayev, S., Zhubatkanov, A., Zhumalin, A., Turbekova, A., Amantaev, B., Lopato, S., Schramm, C., and Jenkins, C. (2019). Intracellular vesicle trafficking genes, RabC-GTP, are highly expressed under salinity and rapid dehydration but down-regulated by drought in leaves of chickpea (Cicer arietinum L.). Front. Genet., 10.","DOI":"10.3389\/fgene.2019.00040"},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Kuzbakova, M., Khassanova, G., Jatayev, S., Daniyeva, N., Sweetman, C., Jenkins, C.L.D., Soole, K.L., and Shavrukov, Y. (2025). Longer internode with same cell length: LcSOC1-b2 gene involved in height to first pod but not flowering in lentil (Lens culinaris Medik.). Plants, 14.","DOI":"10.3390\/plants14081157"},{"key":"ref_80","unstructured":"(2025, December 01). ABI Guide. Available online: https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/cms_042380.pdf."},{"key":"ref_81","unstructured":"NCBI (2025, December 01). LOC123105253, Actin, Available online: https:\/\/www.ncbi.nlm.nih.gov\/gene\/123105253."},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Hewitt, G.M., Johnston, A.W.B., and Young, J.P.W. (1991). DNA protocols for plants. Molecular Techniques in Taxonomy. NATO ASI Series. Vol. 57, Springer.","DOI":"10.1007\/978-3-642-83962-7"},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Arinov, B., Khassanova, G., Zailasheva, A., Kuzbakova, M., Mussynov, K., Sereda, T., Kipshakbayeva, A., Jatayev, S., Tolenova, K., and Sweetman, C. (2025). Genotyping of Le and Af haplotypes in dry pea (Pisum sativam L.) with field trials: Short and semi-leafless plants are not always better in Kazakhstan. Plants, 14.","DOI":"10.3390\/plants14223479"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"575","DOI":"10.2135\/cropsci1985.0011183X002500030046x","article-title":"Registration of \u2018Bozoisky-Select\u2019 Russian wildrye","volume":"25","author":"Asay","year":"1985","journal-title":"Crop Sci."},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Wang, Q., Zhang, T., Cui, J., Wang, X., Zhou, H., Han, J., and Gislum, R. (2011). Path and ridge regression analysis of seed yield and seed yield components of Russian wildrye (Psathyrostachys juncea Nevski) under field conditions. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0018245"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1093\/aob\/mcad056","article-title":"Silica deposition in plants: Scaffolding the mineralization","volume":"131","author":"Zexer","year":"2023","journal-title":"Ann. Bot."},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Lux, A., Luxov\u00e1, Z., Vacul\u00edk, M., \u0160vubov\u00e1, R., Kohanov\u00e1, J., Soukup, M., Martinka, M., and Bokor, B. (2020). Silicification of root tissues. Plants, 9.","DOI":"10.3390\/plants9010111"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"6703","DOI":"10.1093\/jxb\/eraa301","article-title":"Significance of silicon uptake, transport, and deposition in plants","volume":"71","author":"Mandlik","year":"2020","journal-title":"J. Exp. Bot."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1007\/s11103-012-9892-3","article-title":"Cloning, functional characterization and heterologous expression of TaLsi1, a wheat silicon transporter gene","volume":"79","author":"Montpetit","year":"2012","journal-title":"Plant Mol. Biol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.plaphy.2021.04.013","article-title":"Physiological and molecular insights involved in silicon uptake and transport in ryegrass","volume":"163","author":"Pontigo","year":"2021","journal-title":"Plant Physiol. Biochem."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1038\/nature05964","article-title":"An efflux transporter of silicon in rice","volume":"448","author":"Ma","year":"2007","journal-title":"Nature"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1104\/pp.111.173088","article-title":"High-resolution secondary ion mass spectrometry reveals the contrasting subcellular distribution of arsenic and silicon in rice roots","volume":"156","author":"Moore","year":"2011","journal-title":"Plant Physiol."},{"key":"ref_93","doi-asserted-by":"crossref","unstructured":"Fleck, A.T., Schulze, S., Hinrichs, M., Specht, A., Wa\u00dfmann, F., Schreiber, L., and Schenk, M.K. (2015). Silicon promotes exodermal Casparian band formation in Si-accumulating and Si-excluding species by forming phenol complexes. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0138555"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/BF01403298","article-title":"Subcellular localization of mineral deposits in the roots of wheat (Triticum aestivum L.)","volume":"151","author":"Hodson","year":"1989","journal-title":"Protoplasma"}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/16\/9\/1351\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T16:31:29Z","timestamp":1789576289000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/16\/9\/1351"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,16]]},"references-count":94,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["biom16091351"],"URL":"https:\/\/doi.org\/10.3390\/biom16091351","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,16]]}}}