{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:54:05Z","timestamp":1791269645505,"version":"4.1.0"},"reference-count":62,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2015,7,16]],"date-time":"2015-07-16T00:00:00Z","timestamp":1437004800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["R01HG003827"],"award-info":[{"award-number":["R01HG003827"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["R01GM076360-04S1"],"award-info":[{"award-number":["R01GM076360-04S1"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["R01HD065122"],"award-info":[{"award-number":["R01HD065122"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>A key step leading to influenza viral infection is the highly specific binding of a viral spike protein, hemagglutinin (HA), with an extracellular glycan receptor of a host cell. Detailed and timely characterization of virus-receptor binding profiles may be used to evaluate and track the pandemic potential of an influenza virus strain. We demonstrate a label-free glycan microarray assay platform for acquiring influenza virus binding profiles against a wide variety of glycan receptors. By immobilizing biotinylated receptors on a streptavidin-functionalized solid surface, we measured binding curves of five influenza A virus strains with 24 glycans of diverse structures and used the apparent equilibrium dissociation constants (avidity constants, 10\u2013100 pM) as characterizing parameters of viral receptor profiles. Furthermore by measuring binding kinetic constants of solution-phase glycans to immobilized viruses, we confirmed that the glycan-HA affinity constant is in the range of 10 mM and the reaction is enthalpy-driven.<\/jats:p>","DOI":"10.3390\/biom5031480","type":"journal-article","created":{"date-parts":[[2015,7,16]],"date-time":"2015-07-16T10:11:44Z","timestamp":1437041504000},"page":"1480-1498","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Characterization of Receptor Binding Profiles of Influenza  A Viruses Using An Ellipsometry-Based Label-Free Glycan Microarray Assay Platform"],"prefix":"10.3390","volume":"5","author":[{"given":"Yiyan","family":"Fei","sequence":"first","affiliation":[{"name":"Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-Precision Optical Manufacturing, Fudan University, 220 Handan Road, Shanghai 200433, China"},{"name":"Department of Physics, University of California, Davis, CA 95616,   USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yung-Shin","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Physics, University of California, Davis, CA 95616,   USA"},{"name":"Department of Physics, Fu-Jen Catholic University, New Taipei City   24205, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of California, Davis, CA 95616,   USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of California, Davis, CA 95616,   USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kam","family":"Lau","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of California, Davis, CA 95616,   USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Landry","sequence":"additional","affiliation":[{"name":"Department of Physics, University of California, Davis, CA 95616,   USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeng","family":"Luo","sequence":"additional","affiliation":[{"name":"Center for Comparative Medicine, University of California, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Baumgarth","sequence":"additional","affiliation":[{"name":"Center for Comparative Medicine, University of California, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of California, Davis, CA 95616,   USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangdong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Physics, University of California, Davis, CA 95616,   USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2015,7,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1128\/CMR.14.1.129-149.2001","article-title":"Pandemic threat posed by avian influenza A viruses","volume":"14","author":"Horimoto","year":"2001","journal-title":"Clin. 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